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Aram Jeong,Beom-Chan Park,Hee-Yeon Kim,최준용,천진홍,Joung-Hyun Park,Bae-Jin Lee,김기봉 한국한의학연구원 2021 Integrative Medicine Research Vol.10 No.2
Background: Some experimental studies have established the effect of oysters on the promotion of body growth. Yet, there is a lack of human clinical studies. The objective of this study was to evaluate the effect of a fermented oyster (FO) extract on the increase in the height of children with stature in the 25th percentile by age. Methods: In total, 100 children (6–11 years old) were randomly divided into two (FO or control) groups. For 24 weeks, the subjects in the FO group took the FO extract once daily before sleeping, whereas the control group took placebo extracts, simultaneously. We evaluated the height gain, height velocity (HV), height standard deviation score (SDS), urine deoxypyridinoline (DPD), growth hormone (GH), insulin-like growth factor (IGF-1), and IGF binding protein 3 (IGFBP-3). Results: The height gain and height SDS were significantly higher in the FO group than in the placebo group after 24 weeks (height gain: p < 0.001, height SDS: p < 0.005). The HV was also significantly higher in the FO group than in the placebo group after the 6th and 24th week (p = 0.001, p = 0.004). After 24 weeks, we observed a decrease in GH, IGF, and IGFBP-3 in both groups. However, serum IGFBP-3 level in the FO group reduced less than placebo group. Conclusion: FO supplementation may help to increase the height of children, and the effect might be mediated via effects on the IGFBP-3 levels.
Birth seasonality in Korean Prader-Willi syndrome with chromosome 15 microdeletion
Aram Yang,Yeon Hee Lee,Soon Young Nam,Yu Ju Jeong,Ye-Chan Kyung,Rimm Huh,Jieun Lee,Younghee Kwun,Sung-Yoon Cho,진동규 대한소아내분비학회 2015 Annals of Pediatirc Endocrinology & Metabolism Vol.20 No.1
Purpose: Prader-Willi syndrome (PWS) is a well-known genetic disorder, and microdeletion on chromosome 15 is the most common causal mechanism. Several previous studies have suggested that various environmental factors might be related to the pathogenesis of microdeletion in PWS. In this study, we investigated birth seasonality in Korean PWS. Methods: A total of 211 PWS patients born from 1980 to 2014 were diagnosed by methylation polymerase chain reaction at Samsung Medical Center. Of the 211 patients, 138 were born from 2000–2013. Among them, the 74 patients of a deletion group and the 22 patients of a maternal uniparental disomy (UPD) group were compared with general populations born from 2000 using the Walter and Elwood method and cosinor analysis. Results: There was no statistical significance in seasonal variation in births of the total 211 patients with PWS (χ2=7.2522, P=0.2982). However, a significant difference was found in the monthly variation between PWS with the deletion group and the at-risk general population (P<0.05). In the cosinor model, the peak month of birth for PWS patients in the deletion group was January, while the nadir occurred in July, with statistical significance (amplitude=0.23, phase=1.2, low point=7.2). The UPD group showed the peak birth month in spring; however, this result was not statistically significant (χ2=3.39, P=0.1836). Conclusion: Correlation with birth seasonality was identified in a deletion group of Korean PWS patients. Further studies are required to identify the mechanism related to seasonal effects of environmental factors on microdeletion on chromosome 15.
( Aram Yoon ),( Jun Young Hong ),( Soon-mi Choi ),( Dong Woo Hahn ),( Ki Jeong Kim ),( Suk Ho Lee ) 한국운동생리학회 2015 운동과학 Vol.24 No.3
PURPOSE: The present study was designed to evaluate the effects of different resistance protocols on muscle mass and function in rats. METHODS: Thirty two Sprague-Dawley female rats, 8 weeks of age, were randomly divided into 4 groups (n=8 in each group): control (CON), resistance training 1 (RT1; 6 to 8 reps/1 set, every 3rd day), resistance training 2 (RT2; 5 reps/3 sets, every other day), resistance training 3 (RT3; 5 reps/3 sets, twice a day, every 3rd day). Ladder climbing was used as the resistance training method. RESULTS: Muscle mass and cross sectional area (CSA) of flexor hallucis longus (FHL) and extensor carpi radialis (ECR) were increased in all resistance training groups (p<.05). Grip strength was significantly increased in RT3 group (p<.05). Peak twitch tension (Pt) was increased in RT1 and RT3 group, while peak tetanic tension (Po) was increased only in RT3 group (p<.05). There was no change of total protein concentration after training, however, myofibrillar protein contents were significantly increased in FHL and ECR muscles in all training groups. CONCLUSIONS: It is concluded that different resistance training protocol effectively induced the muscle hypertrophy by increasing myofibrillar protein contents. RT1, a low volume progressive single set resistance training protocol, is as effective as traditional high volume multi sets training protocol (RT2 and RT3) for increasing muscle strength in animal model.
( Aram Kang ),( Woonsung Na ),( Minjoo Yeom ),( Hyekwon Kim ),( Sun-woo Yoon ),( Heejun Yook ),( Dae-gwin Jeong ),( Daesub Song ) 대한인수공통전염병학회 2017 창립총회 및 학술대회 초록집 Vol.2017 No.1
Introduction: Middle East respiratory syndrome coronavirus (MERS-CoV), a member of the family of betacoronavirus, was first identified in Saudi Arabia in 2012. MERS-CoV has ability to cross the host species from camel to human causing severe acute respiratory illnesses, and spread by contact in human population. For diagnosis of MERS-CoV in camels, the immunochromatographic assay (ICA) has been used due to its rapid decision and prompt triage of infected animal for the early quarantine. However, when the ICA is applied to an expectorated sputum in which antigens are present, the viscosity of sputum interferes with the migration of the antigens on the test strip. To overcome this limitation, it is necessary to use a mucolytic agent without affecting the antigens. In this study, we have developed a sputum pre-treatment method by testing specimens of the sputa spiked with alphacorona virus and MERS-CoV. Methods: Two mucolytic agents were used: Tris(2-carboxyethyl) Phosphine (TCEP) and N-acetyl-L-cysteine (NALC) and prepared at various concentration to treat with sputum. Bovine serum albumin (BSA) was used as a blocking agent and protease inhibitor cocktail (PI) was used to inhibit the cleavage of the antigens. After treating the compound to sputum, the mixture was applied to colloidal gold-based immunochromatographic test strip for rapid detection of MERS-CoV or alpha coronavirus (BIONOTE Inc., South Korea). The intensities of colloidal gold were measured by MEDISENSOR Gold reader (SD BIOSENSOR Ltd., South Korea). Results: Intensity of test line was higher when the sputa spiked with alpha coronavirus was processed with TCEP, BSA and PI than with TCEP alone. In the case of the sputa spiked with inactivated MERS-CoV, mixture of TCEP and BSA presented higher intensities, while it decreased in the addition of PI. This was reproduced when compound of NALC and BSA was treated to the specimens. Conclusion: In this study, we demonstrated that mixture of the mucolytics, blocking agent and protease inhibitor together effectively dissolve the viscosity of sputum minimizing the effect on antigens, which is more suitable for use in flow immunochromatographic test kit than when sputum or mucolytics alone were used.
이아람 ( Aram Lee ),최경호 ( Kyungho Choi ),김해중 ( Hai-Joong Kim ),이정재 ( Jeong Jae Lee ),최규연 ( Gyuyeon Choi ),김성주 ( Sungjoo Kim ),김수영 ( Su Young Kim ),조금준 ( Geumjoon Cho ),김영돈 ( Youg Don Kim ),서은숙 ( Eunsook 한국산업보건학회 2018 한국산업보건학회지 Vol.28 No.3
Objectives: This study aims to analyze manganese (Mn) concentrations in maternal and cord bloods at delivery and to estimate the Mn exposure risk for fetuses whose mothers were occupationally exposed to Mn. Materials and Methods: Forty-six pairs of maternal and cord blood samples were collected at delivery from mothers who were occupationally unexposed to Mn. Mn concentrations of blood were analyzed by graphite furnace atomic absorption spectrometer. Mn exposure levels for fetuses of female workers were estimated by simulating two working exposure scenarios. Results: The geometric mean concentration of Mn in maternal and cord blood were 27.0(1.34) ㎍/L, 46.6(1.25) ㎍/L, respectively. Transfer ratios of Mn from maternal to cord blood were 1.81±0.62, which indicated that the Mn concentrations in cord blood were higher than those in maternal blood. Mn concentrations in cord blood for the worse or general scenarios were estimated to 22.3-1,881 ㎍/L and 1.59-308 ㎍/L, respectively. The probabilities of exceeding 74 ㎍/L, which was adopted as a reference level reported in a previous study, were 95% and 44% for the two scenarios, respectively. Conclusions: Comparable levels of Mn exposure in maternal or cord blood to those in this study have shown various health effects in previous studies. This suggests that Mn exposure levels in mothers and fetuese in Korea need to be monitored and managed. In addition, female workers who are occupationally exposed to Mn should be protected from the exposure since their fetuses can be exposed to Mn at risky levels during their pregnancy.
Acceleration of Gastric Tumorigenesis Through MKRN1-Mediated Posttranslational Regulation of p14ARF
Ko, Aram,Shin, Ji-Young,Seo, Jinho,Lee, Kang-Duck,Lee, Eun-Woo,Lee, Min-Sik,Lee, Han-Woong,Choi, Il-Ju,Jeong, Jin Sook,Chun, Kyung-Hee,Song, Jaewhan U.S. Dept. of Health, Education, and Welfare, Publ 2012 Journal of the National Cancer Institute Vol.104 No.21
<P><B>Background</B></P><P>We investigated whether Makorin ring finger protein 1 (MKRN1), an E3 ligase, affects p14ARF-associated cellular senescence and tumorigenesis by posttranslational modification in gastric tumorigenesis.</P><P><B>Methods</B></P><P>A link between MKRN1 and ARF was examined in MKRN1 null mouse embryonic fibroblasts (MEFs) and in human fibroblasts and gastric cancer cells by silencing MKRN1 using small interfering RNA (siRNA) and short hairpin RNA (shRNA). Ubiquitination and proteasomal degradation assays were used to assess p14ARF degradation associated with MKRN1. MKRN1 and p14ARF expression levels were analyzed with immunohistochemistry in malignant and normal tissues from gastric cancer patients and with χ<SUP>2</SUP> tests. The tumor growth of gastric cancer cells stably expressing MKRN1 shRNA, p14ARF shRNA, or both was examined in mouse xenograft models (n = 4–6) and analyzed with unpaired <I>t</I> tests. All statistical tests were two-sided.</P><P><B>Results</B></P><P>MKRN1 knockout MEFs exhibited premature senescence and growth retardation with increased p19ARF protein expression. Similar results were obtained for human fibroblasts or gastric cancer cell lines by MKRN1 knockdown. Biochemical analyses confirmed that MKRN1 targets p14ARF for ubiquitination and subsequent proteasome-dependent degradation. A statistically significant association was shown between MKRN1 overexpression and p14ARF underexpression (<I>P</I> = .016). Xenograft analyses using p53-functional AGS or -dysfunctional SNU601 cells displayed statistically significant tumor growth retardation by silencing MKRN1, which was reversed under depletion of p14ARF (AGS cells, MKRN1 knockdown tumors vs MKRN1 and p14ARF knockdown tumors: 164.6 vs 464.8mm<SUP>3</SUP>, difference = 300.2mm<SUP>3</SUP>, 95% CI = 189.1 to 411.3mm<SUP>3</SUP>, <I>P</I> < .001).</P><P><B>Conclusions</B></P><P>We demonstrated that MKRN1 functions as a novel E3 ligase of p14ARF and that it potentially regulates cellular senescence and tumorigenesis in gastric cancer.</P>