Bacteroides fragilis의 독소 유전자 분석 및 발병기전에 관한 연구

오희복,이근영,정경태,성원근 국립보건연구원 2000 국립보건원보 Vol.37 No.-

Bacteroides fragilis의 독소유전자 분석 및 재조합독소 발현에 관한 연구

오희복,정경태,이기은,성원근,이용진,이근영 국립보건연구원 1999 국립보건원보 Vol.36 No.-

Morphological, ecological and biological variations in the mustard aphid, Lipaphis pseudobrassicae (Kaltenbach) (Hemiptera: Aphididae) from different host plants

B.K. Agarwala,Kalpana Das,Parichita Raychoudhury 한국응용곤충학회 2009 Journal of Asia-Pacific Entomology Vol.12 No.3

Genetic and morphological differentiation of insect populations in relation to the use of different host plants is an important phenomenon that leads to ecological specialization. In this study, we describe variations in morphology, and in ecological and biological parameters of Lipaphis pseudobrassicae (Kaltenbach) clones associated with three host species of Cruciferae, Brassica juncea (L.) var. rai sarson Czern and Cross (brown mustard), Brassica campestris L. var. sarson Prain (yellow mustard), and Rorippa indica (L.) Hiern (wild herb). This study was aimed at obtaining evidence regarding phenotypic differentiation induced by, or associated with, the use of distinct host species. Ten morphological characters, 4 growth parameters and 8 biological functions were investigated in wingless aphids collected from plants of the three host species. Aphids from B. campestris and B. juncea clones were bigger in size, heavier in weight and showed higher growth rates and fecundity than the clones from R. indica. Between the two crop plants, clones from B. juncea showed significantly higher growth rates than the clones from B. campestris. Transfer of L. pseudobrassicae populations from B. campestris to B. juncea and R. indica and vice versa resulted in poor performance. Results indicate that the average phenotype of L. pseudobrassicae individuals inhabiting different host plant species differs as a consequence of the contrasting feeding environments the host species provide. Genetic and morphological differentiation of insect populations in relation to the use of different host plants is an important phenomenon that leads to ecological specialization. In this study, we describe variations in morphology, and in ecological and biological parameters of Lipaphis pseudobrassicae (Kaltenbach) clones associated with three host species of Cruciferae, Brassica juncea (L.) var. rai sarson Czern and Cross (brown mustard), Brassica campestris L. var. sarson Prain (yellow mustard), and Rorippa indica (L.) Hiern (wild herb). This study was aimed at obtaining evidence regarding phenotypic differentiation induced by, or associated with, the use of distinct host species. Ten morphological characters, 4 growth parameters and 8 biological functions were investigated in wingless aphids collected from plants of the three host species. Aphids from B. campestris and B. juncea clones were bigger in size, heavier in weight and showed higher growth rates and fecundity than the clones from R. indica. Between the two crop plants, clones from B. juncea showed significantly higher growth rates than the clones from B. campestris. Transfer of L. pseudobrassicae populations from B. campestris to B. juncea and R. indica and vice versa resulted in poor performance. Results indicate that the average phenotype of L. pseudobrassicae individuals inhabiting different host plant species differs as a consequence of the contrasting feeding environments the host species provide.

Adenosine triphosphate-based chemotherapy response assay-guided chemotherapy in unresectable colorectal liver metastasis

Hur, H,Kim, N K,Kim, H G,Min, B S,Lee, K Y,Shin, S J,Cheon, J H,Choi, S H Nature Publishing Group 2012 The British journal of cancer Vol.106 No.1

<P><B>Background:</B></P><P>This study aims to evaluate the effectiveness of adenosine triphosphate-based chemotherapy response assay (ATP-CRA)-guided neoadjuvant chemotherapy for increasing resectability in patients with unresectable colorectal liver metastasis.</P><P><B>Patients and methods:</B></P><P>Patients were randomised into two groups: Group A was treated by conventional chemotherapy regimen and Group B was treated by chemotherapy regimen according to the ATP-CRA. Three chemotherapeutic agents (5-fluorouracil, oxaliplatin and irinotecan) were tested by ATP-CRA and more sensitive agents were selected. Either FOLFOX or FOLFIRI was administered. Between Group A and B, treatment response and resectability were compared.</P><P><B>Results:</B></P><P>Between November 2008 and October 2010, a total 63 patients were randomised to Group A (<I>N</I>=32) or Group B (<I>N</I>=31). FOLFOX was more preferred in Group A than in Group B (26 out of 32 (81.3%) <I>vs</I> 20 out of 31 (64.5%)). Group B showed better treatment response than Group A (48.4% <I>vs</I> 21.9%, <I>P</I>=0.027). The resectability of hepatic lesion was higher in Group B (35.5% <I>vs</I> 12.5%, <I>P</I>=0.032). Mean duration from chemotherapy onset to the time of liver resection was 11 cycles (range 4–12) in Group A and 8 cycles (range 8–16) in Group B.</P><P><B>Conclusion:</B></P><P>This study showed that tailored-chemotherapy based on ATP-CRA could improve the treatment response and resectability in initially unresectable colorectal liver metastasis.</P>

Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis

Yu, K‐,H.,Hong, K‐,S.,Lee, B‐,C.,Oh, M‐,S.,Cho, Y‐,J.,Koo, J‐,S.,Park, J‐,M.,Bae, H‐,J.,Han, M‐,K.,Ju, Y‐,S.,Kang, D‐,W.,Appelros, P. Blackwell Publishing Ltd 2011 Acta neurologica Scandinavica Vol.123 No.5

<P>Yu K‐H, Hong K‐S, Lee B‐C, Oh M‐S, Cho Y‐J, Koo J‐S, Park J‐M, Bae H‐J, Han M‐K, Ju Y‐S, Kang D‐W, Appelros P, Norrving B, Terent A. Comparison of 90‐day case‐fatality after ischemic stroke between two different stroke outcome registries using propensity score matching analysis. 
Acta Neurol Scand: 2011: 123: 325–331. 
© 2010 John Wiley & Sons A/S.</P><P><B>Background – </B> It has not been clarified whether the disparity in ischemic stroke outcome between populations is caused by ethnic and geographic differences or by variations in case mix. Propensity score matching (PSM) analysis can overcome some analytical problems but is rarely used in stroke outcome research. This study was to compare the ischemic stroke case‐fatality between two PSM cohorts of Sweden and Korea.</P><P><B>Methods – </B> Prognostic variables related to baseline characteristics and stroke care were included in our PSM model. Then, we selected 7675 Swedish and 1220 Korean patients with ischemic stroke from each stroke registers and performed one‐to‐one matching based on propensity scores of each patient.</P><P><B>Results – </B> After PSM, all measured variables were well balanced in 1163 matched subjects, and the 90‐day case‐fatality was identical 6.2% (HR 0.997, 95%CI 0.905–1.099) in Sweden and Korea.</P><P><B>Conclusions – </B> No difference is found in the 90‐day case‐fatality in propensity score‐matched Swedish and Korean patients with ischemic stroke.</P>

紅疫백신 製造에 관한 硏究

金慶浩,白承福,李英桂,鄭義範 國立保健硏究院 1967 국립보건연구원보 Vol.4 No.-

EFFECTS OF DIETARY VITAMIN B₆ LEVELS ON LIPID CONCENTRATION AND FATTY ACID COMPOSITION IN GROWING CHICKS

An, B.K.,Tanaka, K.,Ohtani, S. Asian Australasian Association of Animal Productio 1995 Animal Bioscience Vol.8 No.6

This experiment was designed to evaluate the effect of various dietary vitamin $B_6$ levels on conversion from linoleic acid to arachidonic acid in various tissues in growing chicks. Growing chicks were fed the purified diet containing 7% safflower oil with different levels of vitamin $B_6$ (0, 4, 8, 40, 80 mg per kg diet) for 14 days. Feed intake and weight gain in chicks fed the vitamin $B_6$-free diet were markedly depressed. Esterified and free cholesterol concentrations in serum were significantly higher, while the serum triglyceride concentration was significantly lover in chicks fed the vitamin $B_6$-free diet compared to that fed diets with vitamin $B_6$. The liver triglyceride content was also lower in chicks fed the vitamin $B_6$-free diet. The liver and serum cholesterol ester fractions in chicks fed the vitamin $B_6$-free diet showed higher rate of $C_{18:2n6}$ and lower rates of $C_{18:3n6}$, $C_{20:3n6}$ and $C_{20:4n6}$ as compared with vitamin $B_6$ fed groups. In serum phospholipid fraction of chicks fed the vitamin $B_6$-free diet, rates of $C_{20:3n6}$ and $C_{20:4n6}$ were markedly lower. As dietary vitamin $B_6$ level was increased, the rate of $C_{20:4n6}$ was slightly increased, although it was statistically not significant. The fatty acid compositions of adipose tissue showed almost the same pattern as those in liver and serum. This result suggests that the desaturation of $C_{18:2n6}$ to $C_{18:3n6}$, elongation to $C_{20:3n6}$ or both steps might be impaired by vitamin $B_6$ deficiency in growing chicks.

Effect of potassium addition on bimetallic PtSn supported θ-Al₂O₃ catalyst for n-butane dehydrogenation to olefins

Nagaraja, B.M.,Jung, H.,Yang, D.R.,Jung, K.D. Elsevier Science Publishers 2014 CATALYSIS TODAY - Vol.232 No.-

PtSn/θ-Al<SUB>2</SUB>O<SUB>3</SUB> catalysts with different amount of potassium (0.4, 0.7, 0.95, 1.2 and 1.45wt.%) were prepared by an impregnation method, and their catalytic activity in n-butane dehydrogenation was investigated at 823K, an atmospheric pressure and a GHSV of 18,000mL(g<SUB>cat</SUB>h)<SUP>-1</SUP>. The compositions listed in order of n-C<SUB>4</SUB><SUP>=</SUP> yields at 823K were as follows: K<SUB>0.95</SUB>(PtSn)<SUB>1.5</SUB>>(PtSn)<SUB>1.5</SUB>>K<SUB>0.4</SUB>(PtSn)<SUB>1.5</SUB>>K<SUB>0.7</SUB>(PtSn)<SUB>1.5</SUB>>K<SUB>1.2</SUB>(PtSn)<SUB>1.5</SUB>>K<SUB>1.45</SUB>(PtSn)<SUB>1.5</SUB>>K<SUB>0.9</SUB>(Pt)<SUB>1.5</SUB>. The K<SUB>0.9</SUB>(Pt)<SUB>1.5</SUB> and K<SUB>0.95</SUB>(Sn)<SUB>1.5</SUB> catalyst severely deactivated in n-butane dehydrogenation. The (PtSn)<SUB>1.5</SUB> (without K) catalyst showed the highest n-butane conversion, while K<SUB>0.95</SUB>(PtSn)<SUB>1.5</SUB> did the highest n-C<SUB>4</SUB><SUP>=</SUP> yield. The small amount of potassium on bimetallic PtSn/θ-Al<SUB>2</SUB>O<SUB>3</SUB> catalyst improved n-C<SUB>4</SUB><SUP>=</SUP> selectivity, but slightly decreased n-butane conversion, resulting in the increase of n-C<SUB>4</SUB><SUP>=</SUP> yield. The effect of potassium was caused by blocking the acid sites of Pt catalyst. The TPR and HAADF STEM-EDS study suggested the reduction procedure of the Pt, Sn and K species. However, the higher loaded potassium (1.2 and 1.45wt.%) doped (PtSn)<SUB>1.5</SUB> catalysts were rather highly deactivated because the sizes of Pt particles were increased by weakening the interaction between Pt and Sn. The n-C<SUB>4</SUB><SUP>=</SUP> selectivity of the (PtSn)<SUB>1.5</SUB> catalyst increased with respect to the reaction, while that of the potassium doped catalysts maintained the high n-C<SUB>4</SUB><SUP>=</SUP> selectivity from the beginning of the reaction. Also, different alkali metals (Ca, Na and Li) were tested for the comparison with K. The potassium doped catalyst showed the highest n-C<SUB>4</SUB><SUP>=</SUP> yield among the other alkali metals for n-butane dehydrogenation.

Urea와 K₂SO₄ 처리에 의한 복숭아 '미백도'에서 수확 시 과실의 무기성분 농도 및 과피색 변화

문병우,윤익구,문영지,남기웅,이영철,Moon, B.W.,Yoon, I.K.,Moon, Y.J.,Nam, K.W.,Lee, Y.C. 국립한국농수산대학교 교육개발센터 2013 현장농업연구지 = Journal of practical agricultural resear Vol.15 No.1

This study has been conducted to investigate the effect of Urea and K₂SO₄ treatment at stone hardening stage and 20 days before harvest on soil chemical properties, mineral nutrient concentration and quality of 'Mibaekdo' fruit peach. K concentration after Urea and K₂SO₄ treatment in soil was increased significantly by Urea 162g+K₂SO₄ 188g/tree(standard amount) treatment at stone hardening stage, K₂SO₄ 1.0% tree-spray, Urea 81g+K₂SO₄ 94g/tree(half amount), Urea 162g+K₂SO₄ 188g/tree and Urea 324g+K₂SO₄ 376g/tree(double amount) soil treatment before harvest 20 days compared to control. T-N, K and Ca concentration in leaf was increased significantly by all treatment. but Na concentration in leaf was increased by Urea 0.5% and K₂SO₄ 1.0% tree-spray treatment before harvest 20 days. T-N concentration in fruit skin was increased significantly by standard amount soil treatment, which decreased by K₂SO₄ 1.0% tree-spray and half amount soil treatment. T-N, K and Ca concentration in fruit flesh(1~10mm depth flesh from peel) were increased markedly by all treatment excepted Urea 0.5% tree-spray. The leaf weight at harvest was increased markedly by Urea 0.5% tree-spray, standard amount and double amount treatment before harvest 20 days. Fruit weight was increased significantly by standard amount compared to all treatment. Red fruit skin(Hunter a value) progress was effective by K₂SO₄ tree-spray, half amount and double amount treatment before harvest 20 days. Fruit SSC was increased significantly by Urea 0.5% and K₂SO₄ tree-spray before harvest 20 days, standard amount treatment at stone hardening stage compared to control.

반추위내 (反芻胃內) Cellulolytic bacteria 의 분리 동정 (同定) 및 Activity 에 관한 연구 (2) 반추위내 Cellulolytic bacteria 의 Activity 에 관한 연구

민병렬(B . R . Min),맹원재(W . J . Maeng),조남기(N . K . Cho) 한국영양사료학회 1991 韓國營養飼料學會誌 Vol.15 No.3