천연물이 간대사에 미치는 영향에 관한 연구(Ⅱ)

최기환,김순선,박윤주,안미령,서수경,신윤용,김동섭,장영섭 식품의약품안전청 1997 식품의약품안전청 연보 Vol.1 No.-

천연물이 간대사에 미치는 영향페 대한 연구의 일환으로 세계 전 지역에서 널리 사용되고 잇으며 우리나라에서도 사용빈도가 높으나 간염등 간질환 유발사레가 보고되고 있는 마황, 황금 및 샐제 임상에서 이들 생약과 복합해서 자주 사용죄고 있는 대황을 선정하여, 띠들 천연물의 투여가 간 대사에 미치는 영향을 살펴보았다. 대황,마황, 황금 수침액을 1.09/kg의 용량으로 럿드에 7일간 경구 투여하고,최종투여 24시간 후 랫드의 간을 적출하였다. 간 대사에 미치는 영향을 살펴보기 위한 모질약뿔인 7-etliokycournarin을 적출간에 과관류하면서 2시간동안 일정시간 간격으로 관류액을 채취하여 생성된 7-ethoxycoumarin의 대사체인 7-hydroxycoin, glucuronide 포합체, sulfate포합체를 대조군과 배교 관찰하였다. 또한 긴독성 지표로서 혈청 ALT, AST를 측정하였으며 적출관류간에 대해 lipid peronidation 정도를 살펴보고 ÷t직병리검사를 실시하였다. 대촹 투여군에서는 7-ethoxycoumarin의 gulcuronidation이 갛소하였고fP<0.01), 마황투여군에서는 7-ethoxycoumarin의 o-deethylation이 증가하였다(P<0.01). 이러한 7-ethoxycoumariu 대사의 변화가 대황, 마황에 의한 관련 효소의 생합성 증가/감소에 의한 것인지 여부를 살펴보고자 일착로 CYPIAI, Ct'P2Bl CDHA primer를 사용하여 적출관류간 소포체에서 mRNA level을 측정하였다.마황투옥군에서 CVPIAI mRNA level의 증가가 관찰되었지만 통계적으로 유의한 차이를 나타내지는 않았다. 또한 이들 천연물에 의한 간독성 유발여부를 비교 평가한 결과 혈청 ALT 및 AST는 대조군에 비하여 유의한 차이가 없었다. 그러나 만성 간염정도를 나타내는 지표인 ALT/AST ratio는 마황 투여군에ㅓ 대조군에 비하여 유으한 차이를 나타내었고 대황투여군에서 간소포체내 lipid Peroxidation(MDA production)이 대조군에 비해 증가하였다.반면 조직학적 관찰결과는 대조군에 비하여 유의한 차이를 나타내지 않았다. 이상의 결과를 요약하면 랫드에서 대황 투여에 의해 7-ethoxycoumarin gtucuronidation이 감소되었으며, 마황 투여에 의해서 7-ethoxycoumarin의 o-deethylation은 증가되었고 마창 투여군의 경우 이러한 효소의 활성증가는 CYPIA1 induction에 의한 것일 수도 있다는 가능성을 제시하고 있다. In recent 3rears, hepatotoxicity concerned with Ephedrae herba or Scutellariae radixadmiBistration was case reported and Rhei rhizoma is commonly used with them. Tn order to study theeffect of Rhei rhizoma, Ep]ledrae herba and Scutellariae radix on hepatic metabolism, we exalnined theeffect of those pretreatment on the metabolism of 7-ethoxycoumarin(EC). Water extracts(Ig/fg) ofRhei rhiaoma, Ephedrae tterba and Scutetlariae radix were admi3tistered orally to rats for T days,respectively. Livers were t:ten isolated and perfused with 100uM EC for 2 hours. The metabolites of EC,7-hydroxycoernarin, sulfate conjugate and glucuronide conjugate, were measured in the perfusates dur-ing perfusion. The amount of glucuronide conjvgates was decreased iB ahei rhizoma pretreated rats (p <0.01) and 7-bfdroxycournarin was increased in Ephedrae herba pretreated rats(p < 0.01). To examineTrhether the change of enByme activity is related to the induction ,or inhibition of enzymes concerned,we measured the change oif CYPIAt and CYPaBl mRNA level in the perfused rat liver, which are con-sidered to be EC specifie. However, CYPIAI and CYPEBI rnRNA ilevel were not found to be changedwith Rhei rhizoma nor I]3hedrae herba pretreatmen·t. We also assessed the hepatic toxicity of Rheirhizoma,.:phedrae herba and Scutellariae radix. The activity of ALT and AST was assayed at 34hrsafter 7 dfyt ndrninistratiofl and it was not found to be changed- Only the ratio of ALT over AST was in-creased in Epedrae herbs. pretreated rats(p < 0.05), which implies possible chronic hepatitis. Lipidperoxidation was increased in Rhei rhizoma treatment(p <0.05) , while histopathological examinationperformed after liver perfusion did not show any difference compared with vehicle treatmeut. Theseresutts suggest that Ephedrae herba pretreatment increases the o-deethylation of 7- ethoxrcoumariB inrats, which Inay be mediated by CYPIAI mRNA induction.

배지의 무기염류와 sucrose 농도가 감자의 기내 shoot 증식과 기내경 삽목묘의 생존율에 미치는 영향

김희곤,임순희,안장순,송창길,박미경,강봉균 全南大學校 農業科學技術硏究所 1999 農業科學技術硏究 Vol.34 No.-

감자의 virus-free 기내경 삽목묘의 효율적인 대량 생산 체계의 확립을 위하여 생장점 배양으로 얻어진 감자의 기내경의 증식과 기내경의 순화삽목을 실시한 결과는 다음과 같다. 초대배양에서는 염류농도와 sucrose 농도가 높을수록 양호하였다. 즉, MS나 2MS에 sucrose를 6% 첨가한 배지에서 튼튼한 기내경이 생산되었다. 계대배양에서는 sucrose 농도가 높을수록 줄기의 길이는 단축되고 굵기는 커졌으며 생체중은 염류농도와 관계없이 3%의 sucrose가 첨가된 배지에서 무거웠다. 이들 유식물체의 경절을 삽목한 결과 계대배양 배지의 sucrose농도가 6%인 배지에서 생존율이 높은 경향이었다. In order to establish a mass propagation system of potato planting material, in vitro multiplication of virus-free shoot originated from meristem culture and sucrose concentrations on survival of cutting of the in vitro stem tried with various media containing different levels of MS salts(0.5x, 1x and 2x) and sucrose(1.0, 3.0, 6.0%), and the effects of MS salts elements were evaluated. Higher level of sucrose in the initial culture media favored the shoot growth, that is, both of 1MS and 2MS media containing 6.0% sugar produced stouter shoots. Higher level of sucrose in the subculture media also favored the development of compact stout shoots, but biomass production in terms of fresh weight was high on the media containing 3.0%sucrose, regardless of MS salts concentration. The survival rate of cuttings made of the in vitro stem segments tended to be higher in those obtained from subculture media containg 6.0%sucrose.

소규모 농업용 저수지의 저류량-용수공급능력 결정에 관한 연구

안승섭,정순돌,이증석,윤경덕,장인수 한국환경과학회 2002 한국환경과학회지 Vol.11 No.12

This study aims at the effective estimation of water supply capacity of small scale reservoir and the proposal of the data which is necessary to establish the water resources management plan of down stream area of the reservoir in the future by comparison and examination about reservoir operation technique for the security of agricultural water in small scale reservoir. The result of flow calculation by Tank model is used for the input data as the inflow data which is needed for the analysis of water supply capacity. Stochastic method, simulation method, and optimization method are used to examine the water supply capacity, and water security amount is compared with each method. From the analyses of water supply capacities by each method, slightly different results are shown in spite of the effort to compare them equally using input data such as inflow data under equal conditions, and the comparison of water supply capacities by each method are follows; linear planning method, and transition probability matrix method in the order of amount from the largest. It is thought that the simulation method in which comparatively reasonable application of the inflow data is possible and is simulated in successive time series dam operation of the three methods used in this study thus, simulation model is proper to estimate the water supply capacity of agricultural small scale reservoir. And it is judged that the heightening of efficiency of water resources utilization according to the development of downstream area of dam may be possible using the upward readjusted water supply amount of 55.18×10^6ton at 95% and 90% supply reliability respectively which are above the planning water supply amount of 50.0×10^6ton when the simulation method is introduced as the standard.

삼출성 중이염 환자에서의 혈중 및 아데노이드 면역글로불린 E의 면역혈청학적 연구

장혁순,강성호,유창수,김성현,이병돈,박영진,윤세동,안희영,강주원 순천향의학연구소 1997 Journal of Soonchunhyang Medical Science Vol.3 No.1

Otitis media with effusion (OME) is one of the most common disease in children but its pathogenesis is not clear. In particular the role of allergy has revealed conflicting support with widely varing opinions as to its importance. Within recent years, striking biochemical and immunochemical analytic techniques have opened a new approach the study of otitis media with effusion. So we focused on distributions of IgE in adenoid tissue and serum IgE levels. Authors compared the serum IgE and the distribution of IgE in adenoid tissue between the 20 patients underwent tonsillectomy, adenoidectomy and ventilating tube insertion and control group of 11 patients underwent tonsillectomy and adenoidectomy. The results were as follows; 1. In the distribution of IgE in adenoid tissue, there was a significant increase in the group of patients with OME compared to the control group (P<0.01). 2. In the analysis of serum IgE levels, the levels of IgE in the OME patients an control group were higher than normal level, although the level of IgE in the OME patients were less than that of control group, there was no significance P>0.05).

지역사회를 대상으로 한 혈압검진의 결과분석

안종호,신찬수,김성연,박영배,조보연,이홍규,고창순 대한고혈압학회 1997 대한고혈압학회지 Vol.3 No.2

뽕잎머핀에 대한 관능평가 및 이화학적 특성

안창순,여정숙 동아시아식생활학회 2004 동아시아식생활학회지 Vol.14 No.6

This study was carried out to evaluate the sensory evaluation of the muffins with mulberry leaf powder as well as their chemical characteristics. The muffins were prepared by adding 0%, 1%, 2% and 3% mulberry leaf powder to the recipe. As a result of the sensory evaluations, the color, flavor and texture of the muffins of 2% mulberry leaf powder were found to be much better than those of the other groups. In the chemical composition analysis, increasing the mulberry leaf powder led to a significant increase in the content of crude protein, moisture and crude ash, while crude fat was significantly decreased. As the ratio of mulberry leaf powder increased, Ca, Mg, Fe and Zn in the muffins increased proportionally (p<0.05). The lightness value, the redness value and the yellowness value decreased with the increase of mulberry leaves powder content. In a texture analyser test, hardness and cohesiveness decreased as the ratio of mulberry leaf powder increased. The adhesiveness of the muffins with 2% mulberry leaf powder was the strongest of all.

食用 갓(Brassica juncea Coss.)의 人工交配 및 葉色의 遺傳

安奬淳,金光秀 全南大學校 農漁村開發硏究所 1980 農業科學技術硏究 Vol.15 No.-

本 硏究는 菜蔬로써의 價値는 높으나 硏究가 거의 되어 있지 않는 食用 갓의 人工交配와 葉色의 遺傳에 關한 品種育成을 爲한 基礎調査이다. 갓에는 綠色, 談紫色(表面 紫色), 濃紫色(兩面紫色)이 있고 그 중 濃紫色의 것이 商品價値가 가장 크다. 갓의 人工交配에서의 平均 結實率은 73% 程度이었고, 갓에는 自家 및 他家不和合性은 認定되지 않았다. 人工交配는 開花 1日前, 2日前, 3日前의 花蓄 모두 實用上 使用이 可能하고 花粉만 求得되면 日中 어느 時核에나 實施할수 있는 것으로 나타났다. 갓의 葉色은 濃紫色이 優性인 單因子에 依한 形質이고 幼苗의 紫色子葉은 濃紫色의 遺傳標識으로 使用될 수 있는 것으로 나타났다. The artificial pollinations and inheritance of leaf color in leaf mustard is reported for the first time. Leaf mustard is a minor vegetable crop in Korea but has long been grown as a valuable salad and spice crop in Chonnam Province. Three types of it are distinguished on the basis of leaf color; green, light purple and dark purple. The dark purple type is the most accepted one in markets. Overall fruit set from artificial pollination averaged 73% and self or cross-incompatibility was not noticed. Flower buds on one, two and three days before flowering were found to be practically equally good for artificial pollination. The fruit sets from artificial pollinations did not very at any time of pollination during the day from 9 am. to 3 pm. The purple leaf color was found to be conditioned by a single dominate gene and purple cotyledons was genetic marker of dark purple leaves.

고체상 미량분석법(SPME)을 이용한 GC/FID에서 PCE 및 TCE 최적 분석법

안상우,이시진,장순웅 한국환경과학회 2004 한국환경과학회지 Vol.13 No.10

A new method based on solid phase microextraction(SPME), coupled with GC/FID, has been developed for the determination of PCE and TCE in water samples. The experimental parameters affecting the SPME process (i.e, kinds of fibers, extraction time, desorption time, extraction temperature, volume ratio of sample to headspace, salt addition, and magnetic stirring) were optimized. The coefficients of determination (R²) for PCE and TCE were 0.9951 and 0.9831, respectively when analytes concentration ranges from 10 to 300μg/L. The relative standard deviations were 3.4 and 2.1% for concentration of l0μg/L(n=5), respectively. The detection limits of PCE and TCE were 0.5 and 1.3μg/L, respectively.

안면비대칭에 대한 주관적 인지도와 정모두부방사선사진 분석치의 연관성

안정순,황현식,김창수,김경근 전남대학교 치의학연구소 2001 구강과학 Vol.13 No.3

This study was aimed how posteroanterior cephalometric measurements affect the perception of the patient about his/her facial asymmetry. One hundred orthodontic patients, over the age of 12, who required a posteroanterior cephalogram in the Department of Orthodontics of Chonnam National University Hospital were used as the subject. They were asked if they thought their faces are asymmetrical. Their responses were classified into 5 groups based on the level of asymmetry as follows : Definitely No, Probably No, Don't Know. Probably Yes, Definitely Yes. Nine linear and four angular measurements from each posteroanterior cephalometric radiographs were analysed on the standard of the line between crista galli and anterior nasal spine to show the extent of asymmetry. Through this comparative study, the following results were obtained. 1. As the deviation of menton and the midline discrepancy of the upper and lower jaws were greater, the perception of patients about their facial asymmetry was higher. 2. All the measurements from the group "Don't Know" showed no statistical difference with those of the groups "Definitely No" or "Probably No." 3. All the measurements from the group "Probably Yes" showed no statistical difference with those of the group "Definitely Yes." The above results suggest that it may be helpful for the orthodontic diagnosis to ask patients' perception of their own facial asymmetry.

노인전문요양시설 건축모델연구 : 디자인 가이드라인

권순정,오은진,안희창 한국의료복지시설학회 2006 의료·복지 건축 Vol.12 No.1