Simultaneous Analysis of Chlorogenic Acid, Epicatechin Gallate, and Rosmarinic Acid from the LOL (Ligularia stenofelphala , Orostachts japonica, Lavandula anfustifola) Cosmetic Composite and its Antioxidative Activity

Ju Eun Kim,Abinash Chandra Shrestha,Youn Jeong Jo,Jae Yoon Leem 한국피부과학연구원 2021 아시안뷰티화장품학술지 Vol.19 No.2

목적: 천연 화장품에 대한 수요가 증가함에 따라 적절한 생산 관리 및 품질 관리가 우선 되어야 한다. Chlorogenic aicd (CGA), epicatechin gallate (ECG) 및 rosmarinic acid (RMA)을 지표성분으로 선택하여 Ligularia stenocephala (LS; 곤달비), Orostachys japonica (OJ; 와송) 및 Lavandula angustifolia (LA; 라벤더) 50 % EtOH 추출물이 함유 된 화장품 조성물 LOL을 검증했다. 방법: High-performance liquid chromatography with diode array (HPLC-DAD)를 사용하여 LOL (Ligularia stenofelphala, Orostachys japonica, Lavandula anfustifolia)의 세 가지 화합물을 동시에 분석했다. HPLC-DAD를 사용하여 선형성, 정확도 및 정밀도 분석 을 수행했다. 그리고 DPPH 라디칼 소거 분석을 통해 항산화 효과를 평가했다. 결과: 세 가지 추출물 모두 DPPH 라디칼 소거 효과 가 높았으며 검출 된 지표 성분은 유의 한 직선성을 나타냈다(R2≥0.9997). CGA, ECG 및 RMA의 검출 한계(LOD)는 각각 0.83 µg/ mL, 0.33 µg/mL 및 0.20 µg/mL이며, CGA, ECG 및 RMA의 정량 한계(LOQ)는 각각 2.51 µg/mL, 0.99 µg/mL 및 0.61 µg/mL이 다. 분석법 검증 결과, CGA, ECG 및 RMA의 허용 가능한 정밀도(일중 및 일간 정밀도)는 각각 1.77, 1.42, 0.80 % 및 1.09, 0.72, 1.42 %이고, 회수율은 각각 102.00 %-117.78 %, 94.40 %-108.06 % 및 93.79 %-107.05 %이다. 결론: 3 개의 지표 성분의 함량 은 각각 13.99 µg/mL CGA, 8.28 µg/m ECG 및 8.40 µg/mL RMA 이다. 또한 본 연구는 개발 된 방법이 LOL 화장품 조성물 품질 관리 프로세스를 검증 할 수 있음을 시사한다 Purpose: As the demand for natural cosmetics increases, properly managing production and quality control should be prioritized. Chlorogenic acid (CGA), epicatechin gallate (ECG), and rosmarinic acid (RMA) were selected to validate the cosmetic composite LOL, a mixture of 50% EtOH extract of Ligularia stenocephala (LS), Orostachys japonica (OJ), and Lavandula angustifolia (LA). Methods: Highperformance liquid chromatography with a diode array detector (HPLC-DAD) was used to simultaneously analyze the three LOL compounds from LOL (Ligularia stenocephala , Orostachys japonica , Lavandula angustifolia ). Linearity, accuracy, and precision analyses were performed using HPLC-DAD, and the anti-oxidative effects were evaluated through a 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical-scavenging assay. Results: All three extracts showed a high DPPH radical-scavenging effect, and the detected marker compounds showed significant linearity (R2≥0.9997). The limit of detection (LOD) of CGA, ECG, and RMA was 0.83 μg/mL, 0.33 μg/mL, and 0.20 μg/mL, respectively. Meanwhile, the limit of quantification (LOQ) of CGA, ECG, and RMA was 2.51 μg/mL, 0.99 μg/mL, and 0.61 μg/mL, respectively. Method validation showed acceptable precision (intra-and inter-day precision, CGA, ECG, and RMA, 1.77, 1.42, 0.80% and 1.09, 0.72, 1.42%, respectively) and recovery (CGA, ECG, and RMA, 102.00%–117.78%, 94.40%-108.06%, and 93.79%–107.05%). Conclusion: The contents of the three markers were 13.99 μg/mL CGA, 8.28 μg/mL ECG, and 8.40 μg/mL of RMA, respectively. This study also suggests that the developed method can validate the LOL cosmetic composition quality control process.

WS-5 Extract of <i> Curcuma longa, Chaenomeles sinensis,</i> and <i> Zingiber officinale</i> Contains Anti-AChE Compounds and Improves <i>β</i> -Amyloid-Induced Memory Impairment in Mice

Kim, Ju Eun,Shrestha, Abinash Chandra,Kim, Hyo Shin,Ham, Ha Neul,Kim, Jun Hyeong,Kim, Yeong Jee,Noh, Yun Jeong,Kim, Su Jin,Kim, Dae Keun,Jo, Hyung Kwon,Kim, Dae Sung,Moon, Kwang Hyun,Lee, Jeong Ho,Jeo Hindawi 2019 Evidence-based Complementary and Alternative Medic Vol.2019 No.-

<P>Alzheimer's disease (AD) is linked to an extensive neuron loss via accumulation of amyloid-beta (A<I>β</I>) as senile plaques associated with reactive astrocytes and microglial activation in the brain. The objective of this study was to assess the therapeutic effect of WS-5 ethanol extract in vitro and in vivo against A<I>β</I>-induced AD in mice and to identify the extract's active constituents. In the present study, WS-5 exerted a significant inhibitory effect on acetylcholinesterase (AChE). Analysis by transmission electron microscopy (TEM) revealed that WS-5 prevented A<I>β</I> oligomerization via inhibition of A<I>β</I><SUB>1-42</SUB> aggregation. Evaluation of antioxidant activities using 1, 1-diphenyl-2-picrylhydrazyl (DPPH) demonstrated that WS-5 possessed a high antioxidant activity, which was confirmed by measuring the total antioxidant status (TAS). Furthermore, the anti-inflammatory properties of WS-5 were examined using lipopolysaccharide-stimulated BV-2 microglial cells. WS-5 significantly inhibited the lipopolysaccharide–induced production of nitric oxide and two proinflammatory cytokines, TNF-<I>α</I> and IL-6. The memory impairment in mice with A<I>β</I>-induced AD was studied using the Morris water maze and passive avoidance test. Immunohistochemistry was performed to monitor pathological changes in the hippocampus and cortex region of the mouse brain. The animal study showed that WS-5 (250 mg/kg) treatment improved learning and suppressed memory impairment as well as reduced A<I>β</I> plaque accumulation in A<I>β</I>-induced AD. HPLC analysis identified the extract's active compounds that exert anti-AChE activity. In summary, our findings suggest that WS-5 could be applied as a natural product therapy with a focus on neuroinflammation-related neurodegenerative disorders.</P>

모과 부탄올 분획의 예쁜꼬마선충 내의 항산화 효과

김준형,안창완,김영지,노윤정,김수진,김수진,Abinash Chandra Shrestha,함하늘,임재윤,조형권,김대성,문광현,이정호,정경옥,김대근 한국생약학회 2018 생약학회지 Vol.49 No.1

Chaenomeles sinensis (Thouin) Koehne fruit (Rosaceae) has been used as a traditional medicine in Korea, Japan and China to treat sore throat, diarrhea and inflammation. The ethanol extract of C. sinensis fruit was successively partitioned as methylene chloride, ethyl acetate, n-butanol and H2O soluble fractions. Among those fractions, the n-butanol fraction showed the most potent DPPH radical scavenging and superoxide quenching activities. To verify antioxidant activities, the n-butanol fraction was checked the activities of superoxide dismutase (SOD) and catalase activities, and intracellular ROS levels and oxidative stress tolerance in Caenorhabditis elegans. Furthermore, to see if increased stress tolerance of worms by treating of the n-butanol fraction was due to regulation of stress-response gene, we quantified SOD-3 expression using transgenic strain. Consequently, the n-butanol fraction elevated SOD and catalase activities of C. elegans, and reduced intracellular ROS accumulation in a dose–dependent manner. Moreover, the n-butanol fraction-treated CF1553 worms exhibited significantly higher SOD-3::GFP intensity.

Inhibition of β-site amyloid precursor protein cleaving enzyme 1 and cholinesterases by pterosins via a specific structure−activity relationship with a strong BBB permeability

Susoma Jannat,Anand Balupuri,Md Yousof Ali,홍승수,최천환,최윤혁,구진모,김우정,임재윤,김주은,Abinash Chandra Shrestha,함하늘,이기호,김동민,강남숙,박길홍 생화학분자생물학회 2019 Experimental and molecular medicine Vol.51 No.-

We extracted 15 pterosin derivatives from Pteridium aquilinum that inhibited β-site amyloid precursor protein cleaving enzyme 1 (BACE1) and cholinesterases involved in the pathogenesis of Alzheimer’s disease (AD). (2R)-Pterosin B inhibited BACE1, acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) with an IC50 of 29.6, 16.2 and 48.1 μM, respectively. The Ki values and binding energies (kcal/mol) between pterosins and BACE1, AChE, and BChE corresponded to the respective IC50 values. (2R)-Pterosin B was a noncompetitive inhibitor against human BACE1 and BChE as well as a mixed-type inhibitor against AChE, binding to the active sites of the corresponding enzymes. Molecular docking simulation of mixed-type and noncompetitive inhibitors for BACE1, AChE, and BChE indicated novel binding site-directed inhibition of the enzymes by pterosins and the structure−activity relationship. (2R)-Pterosin B exhibited a strong BBB permeability with an effective permeability (Pe) of 60.3×10−6 cm/s on PAMPA-BBB. (2R)- Pterosin B and (2R,3 R)-pteroside C significantly decreased the secretion of Aβ peptides from neuroblastoma cells that overexpressed human β-amyloid precursor protein at 500 μM. Conclusively, our study suggested that several pterosins are potential scaffolds for multitarget-directed ligands (MTDLs) for AD therapeutics.

Longevity and Stress Resistant Property of 6-Gingerol from Zingiber officinale Roscoe in Caenorhabditis elegans

Lee, Eun Byeol,Kim, Jun Hyeong,An, Chang Wan,Kim, Yeong Jee,Noh, Yun Jeong,Kim, Su Jin,Kim, Ju-Eun,Shrestha, Abinash Chandra,Ham, Ha-Neul,Leem, Jae-Yoon,Jo, Hyung-Kwon,Kim, Dae-Sung,Moon, Kwang Hyun,L The Korean Society of Applied Pharmacology 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.6

In order to discover lifespan-extending compounds made from natural resources, activity-guided fractionation of Zingiber officinale Roscoe (Zingiberaceae) ethanol extract was performed using the Caenorhabditis elegans (C. elegans) model system. The compound 6-gingerol was isolated from the most active ethyl acetate soluble fraction, and showed potent longevity-promoting activity. It also elevated the survival rate of worms against stressful environment including thermal, osmotic, and oxidative conditions. Additionally, 6-gingerol elevated the antioxidant enzyme activities of C. elegans, and showed a dose-depend reduction of intracellular reactive oxygen species (ROS) accumulation in worms. Further studies demonstrated that the increased stress tolerance of 6-gingerol-mediated worms could result from the promotion of stress resistance proteins such as heat shock protein (HSP-16.2) and superoxide dismutase (SOD-3). The lipofuscin levels in 6-gingerol treated intestinal worms were decreased in comparison to the control group. No significant 6-gingerol-related changes, including growth, food intake, reproduction, and movement were noted. These results suggest that 6-gingerol exerted longevity-promoting activities independently of these factors and could extend the human lifespan.

Longevity and Stress Resistant Property of 6-Gingerol from Zingiber officinale Roscoe in Caenorhabditis elegans

( Eun Byeol Lee ),( Jun Hyeong Kim ),( Chang Wan An ),( Yeong Jee Kim ),( Yun Jeong Noh ),( Su Jin Kim ),( Ju-eun Kim ),( Abinash Chandra Shrestha ),( Ha-neul Ham ),( Jae-yoon Leem ),( Hyung-kwon Jo ) 한국응용약물학회 2018 Biomolecules & Therapeutics(구 응용약물학회지) Vol.26 No.6

In order to discover lifespan-extending compounds made from natural resources, activity-guided fractionation of Zingiber officinale Roscoe (Zingiberaceae) ethanol extract was performed using the Caenorhabditis elegans (C. elegans) model system. The compound 6-gingerol was isolated from the most active ethyl acetate soluble fraction, and showed potent longevity-promoting activity. It also elevated the survival rate of worms against stressful environment including thermal, osmotic, and oxidative conditions. Additionally, 6-gingerol elevated the antioxidant enzyme activities of C. elegans, and showed a dose-depend reduction of intracellular reactive oxygen species (ROS) accumulation in worms. Further studies demonstrated that the increased stress tolerance of 6-gingerol-mediated worms could result from the promotion of stress resistance proteins such as heat shock protein (HSP-16.2) and superoxide dismutase (SOD-3). The lipofuscin levels in 6-gingerol treated intestinal worms were decreased in comparison to the control group. No significant 6-gingerol-related changes, including growth, food intake, reproduction, and movement were noted. These results suggest that 6-gingerol exerted longevity-promoting activities independently of these factors and could extend the human lifespan.