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인진(茵蔯) 에탄올추출물이 ${\alpha}$-MSH로 유도된 과색소 형성에 미치는 영향
신기돈,김대성,이장천,문연자,우원홍,이영철,Shin, Ki-Don,Kim, Dae-Sung,Lee, Jang-Cheon,Mun, Yeun-Ja,Woo, Won-Hong,Lee, Young-Cheal 대한동의생리학회 2009 동의생리병리학회지 Vol.23 No.3
Melanogenesis is induced mainly by ultraviolet radiation of sunlight and ${\alpha}$-Melanocyte stimulation hormone (${\alpha}$-MSH) which binds to a specific G protein coupled receptor. ${\alpha}$-MSH and cAMP-elevating agents are known to melanin syntheisis and dendrite outgrowth. The purpose of this study was to investigate the mechanism of melanogenesis inhibition in B16/F10 cells by ethanol extract of Artemisiae Capillaris Herba. In the present study, ${\alpha}$-MSH led to a stimulation of melanin synthesis that appeared to result from an increased tyrosinase activity and melanin content. However, the ethanol extract of Artemisiae Capillaris Herba inhibited the ${\alpha}$-MSH-induced tyrosinase activity and melanin content. In control conditions, B16/F10 cells displayed a fibroblastic appearance while ${\alpha}$-MSH treatment promoted the emergence of small and numerous dendrites from the plasma membrane. The ethanol extract of Artemisiae Capillaris Herba abolished the ${\alpha}$-MSH-induced dendricity. Regarding protein levels of the melanogenic enzymes, the amounts of tyrosinase were increased after incubation with ${\alpha}$-MSH. The treatment of Artemisiae Capillaris Herba ethanol extract decreased the ${\alpha}$-MSH expression levels of tyrosinase. Based on these findings, it is likely that the ethanol extract of Artemisiae Capillaris Herba exerts its depigmenting effects in B16/F10 cells through the suppression of tyrosinase expression, which are key enzymes for melanogenesis.
Wakeman 式 樹形에서의 補助 鐵線이 S.(Seibel) 9110 葡萄나무 新梢의 自然誘引, 切損, 生長 및 果實의 品質에 미치는 影響
李英喆,崔東龍,金圭來,金善圭,李鎬珍 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.1
Influence of supplemental wiring to standard Wakeman's training system on self-training, breakage, and growth of shoots, and fruit quality of Seibel 9110(S.9110, Verdelet) grapevines was studied. 1.Supplemental wiring increased the rate of self-training of shoots, and 2 to 4 wires were more effective than single wire. 2.Shoot breakage was decreased by the supplemental wiring. 3.Shoot growth tended to increase by the supplememntal wiring, and increase in the number of internodes was recognized only with 1W-15 and 2W-15 treatments. 4.Shoot diameter increased by the supplemental wiring. Pruning weight also was increased with supplemental wiring, especially by 2W-15 and 4W-15-30 treatments. 5.Cluster weight was greatly affected by supplemental wiring, and the effect was enhanced by 2W-15 and 4W-15-30 treatments. Brix degrees(°Bx) tended to increase by supplemental wiring but the effect was noted only by 2W-15 treatment.
金善圭,李大鎭,金吉龍,崔東龍,李英喆 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.1
Influence of moist chilling at 3°C and subsequent germination temperature on seed germination of Parthenocissus tricuspidata (Sieb. & Zucc.) Planch. Was studied. 1.Seed germination of P. tricuspidata increased between 20° and 25°C by prolonged chilling of 8 to 12 weeks. 2.Percent seed germination was higher at 20°C than 25°C, but germination rate was higher at 25°C. 3.Length of time for germination(LTG) and germination span (GS) in days were decreased by prolonged chilling. LTG and GS were also lower at 25°C than 20°C. 4.P. tricuspidata seemed to be a dark germinating species.
金善圭,崔東龍,李英喆 충북대학교 농업과학기술연구소 1990 農業科學硏究 Vol.8 No.1
Effects of kind [Ca(OH)2 and Ca(NO3) 2] and concentration (0.15 and 1.0% ) of Ca and tap water treated for various time (1,2, and 4 days) to Campbell Early (Vitis labruscana B.) cuttings on bud-break, shoot growth, rooting, and respiratory activity were studied. 1.Percent budbreak increased by Ca for 2 days and tap water for 1 day treatments. 2.Prolonged soaking with Ca and water increased shoot growth at early stage. 3.Percent rooting and percent of well rooted cuttings at early stage were increased by various soaking treatments, but the effect was diminished as the application time prolonged. 4.Soaking treatments markedly increased the respiratory activity of the cuttings. Respiratory ac-tivity showed a proportional increase by tap water treatment, while with Ca, respiratory activity increased from 1 to 2 day's treatment regardless of the kind of Ca, and whether remained steady [Ca(NO3) 2] or decreased [Ca(OH) 2], thereafter. 5. Respiratory activity and final percent rooting correlated significantly, and correlation coefficients between respiratory activity and percent budbreak after 8 weeks and shoot length after 2 weeks were highly significant
安光厚,金善圭,崔東龍,李英喆 충북대학교 농업과학기술연구소 1988 農業科學硏究 Vol.6 No.1
Ten grape cultivars with diverse genetic background were subjected to 2 levels of daylength(16-hour and 12-hour day) and 2 levels of temperature range (Table 2) combinations. 1.Daylength played a major role in shoot elongation. Shoot growth was stimulated by long days(16-hour) while inhibited by short days (12-hour). 2.Lower temperature range(22.4 to 26.5℃) was more favorable than higher temperature range(24.8 to 28.7℃ for shoot growth. 3.In 16-hour photoperiods, S.9110, GW 8, GW 10, and Tannat showed vigorous growth at both temperature ranges, Pinot Gris favored the lower temperature ranges ; Aramon, Chasselas Dore, Riesling, and Vidal 256 exhibited a reduced growth irrespective of the temperature regimes. 4.Riesling seemed to be indifferent to daylength and temperature. 5.Long days increased the length and number of internodes, and lower temperature ranges tended to increase the number of nodes. 6.Vigorous growth cycle came 30 to 50 days after treatment, and the growth ceased after 70 days.