현대생물학의 방향

이민재 韓國生物敎育學會 1970 생물교육 Vol.4 No.-

上顎에 發生한 齒牙腫의 一例

李玟宰,鄭國賢 醫藥係社 1965 綜合 醫學 Vol.10 No.9

CIonidine, Methyldopa 및 Prazosin이 몇가지 昇壓物質의 作用에 미치는 影響

李玟宰,高錫太 朝鮮大學校 1987 藥學硏究誌 Vol.9 No.1

In order to investigate the effect of clonidine, methyldopa and prazosin on the pressor actions of norepinephrine, tyramine and angiotensin, this study was performed in rabbits. Clonidine, α₂-receptor agonist, markedly potentiated the pressor action of norepinephrine and slightly potentiated that of tyramine but weakened that of angiotensin. Methyldopa, L-aromatic amino-acid decarboxylase inhibitor, potentiated the pressor action of norepinephrine, whereas it did not influence that of tyramine, and weakened that angiotensin. Prazosin, α₁-receptor blocking agent, inhibited all the pressor actions of norepinephrine, tyramine and angiotensin.

식물의 암종유발에 관한 연구 (제 3보) - Bacteria-free 암종조직의 획득에 있어서 항생제의 효과

이민재,홍순우,최영길 한국미생물학회 1967 미생물학회지 Vol.5 No.2

Up to the present time, there are only three methods by which we can obtain bacteria free crown gall tissue. According to some papers related to this field, the first method is based on the works of Braun(53') who maintained infected plants at 46-47'c for several days. But the method has a problem that very few plants can tolerate this temperature. The second method is based on the well known observation that old tumors appear to be bacteria free at least 1 or 2% of the explants. Also this method is known to us as laborious and time consuming. The third method is the one we were using that was attempting to kill the bacteria with bacteriacidal agent such as Antibiotics. In fact., it is reported that almost complete control of crown gall of tomato was obtained by Blanchard('51) when plants were grown in a nutrient containing Aureomycin(20${\mu}g$/ml) following needle puncture with the gall bacteria. We have been engaged in making the experiment by applying solution of Penicillin, Streptomycin and of Chloramphenicol(Succinate free) to find the strong bacteriacidal agent through the method of disc plate, and to confirm the effect of antimicrobial action through the method of plant tissue culture system without possible injury to the host plant. The result of this report is the fact the strongest bacteriacidal agent among the above three Antibiotics was Chloramphenicol(Succinate free 1000 p.p.m). and that there happened no injury to the tissue cultures in a White's 10X media containing 1000 p.p.m. of Chloramphenicol.

식물의 암종유발에 관한 연구 2 (제 2 ) 에서 도마도 줄기에 유발된 의 Peroxidase Activitiy 에 대하여

이민재,홍순우,최영길 한국미생물학회 1966 미생물학회지 Vol.4 No.2

The relationships between tumor score and peroxidase activities of tomato stems infected with Agrobacterium tumefaciens strain A6Kl, B6, T372 11BNV6, 11BV7 and wounded stem as a control were examined in relation to crown gall tumor development on purpose to study the lignification of tumor tissue which is affected to the development of crown gall tumor. As the previous paper has been mentioned the fact that the induction of tumor tissues were inhibited or limited in the lignified stem of host plant. It was presumed that the activities of peroxidase related to the development of lignification were decreased during the period of tumor development. But the experimental result in this experiment shows that the peroxidase activities of crown gall tumor-tissues infected with the A. tumefaciens strains which are already known as virulent are increasing during four weeks, however, in the strain 11BNV6 and wound the peroxidase activities are decreasing on the second week after the inoculation of the bacteria strains. These results could be explained on the basis of that possible regulatory agents of lignification which were accumulated in tumor tissues, IAA, ascorbic acid, glutathion(GSH) and caffeic acid esters, were postulated to act as antioxidants which has been suggested by Stafford. Total nitrogen contents in relation to crown gall tumor development were determined for the detection of protein synthesis related to the enzyme activities which are increasing in the time of plant growth. Generally six groups are contained the largest amount of nitrogen on the second week after the inoculation of the bacterium. Comparing to the tumor score, it is presumed that the all of enzyme activities including peroxidase in tumor tissues are increasing from the second week through the third week after the inoculation of bacterium and the protein synthesis is stimulated under the most appropriated temperature during the above periods.

韓國人 下顎骨의 緻密骨에 關한 硏究

李玟宰 醫藥係社 1964 綜合 醫學 Vol.9 No.7

식물의 암종유발에 관한 연구 1 (제 1 ) Agrobacterium tumefaciens 의 에 관하여

이민재,홍순우,최영길 한국미생물학회 1966 미생물학회지 Vol.4 No.2

As a part of studies of plant tumor induction, this experiment was prepared for the purpose of studying the ability of tumor induction and the tendency of tumor initiation in some Korean plants using the various Agrobacterium tumefaciens strains. Results obtained from this experiment are as follows. The virulences of five strains used in this experiment were gradually decreased in order of strain A6Kl, B6, 11BV7, T37 and 11 BNV6. Especially strain T37 which is known to the host limited strain showed virulent effect to the most of plants given for the materials as well as strain A6Kl, B 6 and 11BV7. Concerning the grade of tumor development, in plants which has tough stem, for example, Glycine max Meer, tumor induction was not well developed after the inoculation of all strains. Particullary in Ricinus communes Linne all strains showed virulent effect but tumor tissues were declined in relation to the development of lignification. It was also confirmed that the induction of tumor tissues on plants is to delay according to the increase of the age of host plants.

음압에 의한 인체 표피의 팽창 정도 및 형태학적 변화에 관한 연구

이민재,강형철 梨花女子大學校 醫科大學 醫科學硏究所 1996 EMJ (Ewha medical journal) Vol.19 No.3

이화 여자 대학교 의과대학 부속 동대문 병원 백반증 크리닉을 방문한 백반증 환자중 표피이식술을 시행한 11명의 전흉부와 복부 18부위에서 일정한 시간동안 일정한 음압으로 흡입수포를 만들었을 때 표피 면적의 팽창 정도를 측정하고, 흡입으로 팽창된 표피의 형태학적 변화를 주사전자현미경과 투사전자현미경으로 관찰하여 다음과 같은 결과를 얻었다. 표피직경은 흡입 전 평군 7.6mm에서 흡입후 평군 11.0mm로 평균 46.4%, 표피면적은 평균 45.0㎟에서 95.9㎟으로 115.0% 증가하여, 팽창도 증가는 통계학적으로 유의하였다(p<0.05). 형태학적 변화로는 표피두께가 현저히 감소되고 표면과 평행한 방향으로 표피의 기저세포, 핵 및 세포간교가 신장된 소견을 보였으며, 각화세포 및 멜라닌세포의 핵 주변에 공포화 현상을 나타내었다. 결론적으로 표피의 팽창 정도 및 형태학적 변화 측정이 피부 표피의 물리학적 성질과 구조를 이해하는데 도움이 되며, 표피 이식수술시 필요한 정확한 공여부 피부면적을 예측하는데 이용될 수 있으리라 생각된다. Recently, autologous epidermal graft using suction-blister has been used in the treatment of depigmented diseases. The suction-blister is produced by separation of epidermis from dermis by negative pressure, and the separated epidermis is a good source for studying the properties of the epidermis itself. In this day, we measured the pre-expanded control diameter and post-expanded diameter induced by negative pressure in the anterior chest and abdomen of patients and calculated the expansibility of epidermis from the measurements, and studied the morphological changes of the expanded epidermis using scanning electron microscope and transmission electron microscope. As a result, the diameter of epidermis was elongated 46.4%, from a mean of 7.6mm to 11. 0mm and the calculated area was expanded 115.0%, from a mean of 45.0㎟ to 95.9㎟ by suction. Both were statistically significant(p<0.05). And morphologically, the thickness of epidermis was markedly decreased and the basal keratinocytes, their nuclei and intercellular bridges were elongated parallel to the skin surface, and paranuclear vacuolization was observed in keratinocytes andmelanocytes. In conclusion, above results are helpful in understanding the physical property and structure of epidermis and can be used for estimating the exact size of the donor skin needed for epidermal graft.

Optimal salt concentration of vehicle for plasmid DNA enhances gene transfer mediated by electroporation

이민재,Soon-ShinCho,Hyung-SukJang,YoungShinLim,Ji-RanYou,JangwonPark,HearanSuh,Jeong-aKim,박종상,김덕경 생화학분자생물학회 2002 Experimental and molecular medicine Vol.34 No.4

"植物 의 耐寒性에 關한 硏究" Ⅱ報 : 大麥類의 耐寒性과 渗透壓과의 關係에 對하여 Relative to Cold-Resistance and Osmatic Pressure with Barley

李敏載 서울대학교 1954 서울대학교 論文集 Vol.1 No.-

Taking Spring and Autumn barley which show a distinct difference on cold-resistance measuring osmotic pressure under various conditions, pursued the relation between cold-resistance and osmotic pressure. (1) Regardless the degree of cold-resistance, in the growing condition(27℃±2℃) which is adequate to temperature, no difference of osmotic pressure was observable. (2) However, whether the barley is treated under insufficient temperature after growth of grown under such condition, both Spring and Autumn barley show the adaptation to it and make the osmotic pressure raise; but the raising rate is seen higher by Autumn barley. (3) Acordingly, osmotic pressure does not become the direct cause to cold-resistance, but it becomes indirect cause through protoplam property or coll id condition. (4) The raise of osmotic pressure of the plant grown in the low temperature, does not come out by want of water supplied, but it is originated by increasing osmotic pressure caused by low temperature, the plant grown in the low temperature shows the higher osmotic pressure even in the equal water condition, But the raising rate of pressure is more higher by Autumn barley than Spring barley. (5) During the growing period, some amount of potash is applied, the more potash shows the higher osmotic pressure. (6) The barley grown in the sun-shadow shows an inclination of less osmotic pressure that of grown in the sun-light.