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      • 담수산패 Lymnaeidae의 분포, 형태 및 세포학적 연구 : Lymnaeidae in Korea

        金鍾煥,崔信錫,辛昌男 충남대학교 자연과학연구소 1982 忠南科學硏究誌 Vol.9 No.2

        Lymnaeidae snails were collected from the 50 spots out of 63 stations where the basins of Geum River. Youngsan River, the lowest part of Nagdong River and Nam River. The snails were identified four species including Lymnaea auricularia. L. ovata, L. pervia and L. trucatula on the bases of the morphological and cytological observations. L. auricularia was found at 31 stations and the most widely distributed species, and L. pervia was found at 27 stations, however another two species were distributed limited spots in the basin of Geum River. The chromosomes of 4 species of Lymnaea snails were observed in the spermatogenesis and Oo^∞genusis. L. auricularia and L.ovata have the haploid chromosome number 17, and L. pervia has the haploid number 16, but L. truncatula has 18.

      • Bithynia misella Gredler, 1884와 Parafossarulus manchoouricus Bourguignat, 1860의 相異点에 關한 硏究

        金鍾煥 충남대학교 의과대학 지역사회의학연구소 1976 충남의대잡지 Vol.3 No.1

        A study dealt with the distribution, classification and morphology of two species of Bithyniidae; Parafossarulus manchouricus Bourguignat, 1860 and Bithynia misella Gredler, 1884 in Daesan Myeon Haman, Korea. P. manchouricus was found throughout most spots of the area, and it was common in the irrigation canals, drainages and ponds, while B. misella was only collected in the irrigation canals, and is here reported for the first time. Dencities of population, B. misella and P. manchouricus, were 24 and 280 individuals in the same lots (5㎡) of irrigation canal and the proportion of both species was 1 by 11.7. Adult shells of B. misella varied in length 5.89±0.77mm, in width 3.46±0.29mm, and P. manchouricus were 11.19±0.82mm in length and 5.82±0.5mm in width. B. misella was distinguished from young specimens of P. manchouricus by its well rounded whorls, deeply idented suture, mantle color pattern and the inner lip of aperture at the rim.

      • 韓國四大江流域에 있어서 Bithynia misella Gredler, 1884의 分布狀態

        金鍾煥 忠南大學校 環境問題硏究所 1983 環境硏究 Vol.1 No.2

        A survey on the distribution of the fresh water snail Bithynia misella was carried out in the four river basins; Geum River, Nagdong River, Yeongsan River and Han River, from March 1980 to September 1983. 1. B. misella was collected in 19 stations(Geum River 12, Nagdong River 6 and Yeongsan River 1) out of 225 stations examined. 2. The habitat distribution of the snail showed at the irrigation canals and rice peddies and one station of drain, however no snail was found in river or other habitats. 3. The density distribution was generally lower at 10 stations but 5 stations showed higher than other localities among 19 positive stations.

      • Bithyniidae 우렁의 越冬期間中 生存率 調査成績

        金鍾煥,辛大煥 충남대학교 의과대학 지역사회의학연구소 1977 충남의대잡지 Vol.4 No.1

        During winter hibernation, the survival rate of Parafossarulus manchouricus and Bithvnia misella was observed with field collected specimens and refrigerated snails. All specimens were collected at the irrigation canal and drainage Daesan Myeon, Haman Gun from October 1975 to March 1976. The survival percent rate of P. manchouricus was 67.7%, and the rate of B. misella was 80% during October to November before hibernaton. After hibernation in March 1976, the rate of P. manchouricus was 12.1% and the B. misella was 66.7%, while P. manchouricus from the drainage was 44.3%. The snails were refrigerated (4±2℃) for 6 months either dry or wet state. The survival rate of P. manchouricus and B. misella on dry place were 4.0% and 35.5% but the rate of P. manchouricus was 92% on wet state. From the above results, B. misella was survived more than P. manchouricus during hibernating period, and the wet state was favorable condition for snails hibernation.

      • KCI등재후보
      • 錦江上流地域에 있어 다슬기科의 分布에 關한 硏究

        金鍾煥,崔信錫,宋仁植,洪榮杓 충남대학교 자연과학연구소 1982 忠南科學硏究誌 Vol.9 No.1

        The distribution and identification of Semisulcospira species were studied at the 8 stations in the upper streams of Geum River and it's tributatries, and some physicochemical analysis were obtained from all stations. The results are summerized as follows: 1. Four species of Semisulcospira were collected from the 7 stations in the 8 stations, except a station, the Buchang stream. S. coreana was found at all the 7 stations, S. ovulum at the 6 stations, S. globus at the 4 stations, and S. tegulata was found only two stations, but the density of all the snails was generally low in a m^2. 2. Identification of the snail species were based on the external morphology, radula ribbon and central teeth patterns. 3. The chromosome numbers were observed : n = 18 in S. coreana and S. ovulum ; n = 16 in S. globus, however, in S. tegulata the chromosome was not observed. 4. Physicochemical analysis in the aquatic environment were determined from all the 8 stations. The pH, Ca, Mg, SO_4, Na and Cl values in the water varied not widely, but the values of chemical oxidation demand at the 5th station and electric conductivity at the 7th station were higher than those of the another stations.

      • Tinidazole 單回投與法에 依한 Giardia lamblia의 驅蟲效果

        辛大煥,金鍾煥,林定圭 충남대학교 의과대학 지역사회의학연구소 1976 충남의대잡지 Vol.3 No.2

        The prevalence rate of Giardia lamblia revealed 5.5% in this investigation. A total 38 cases of Giardiasis were treated with Tinidazole 50mg per kg body weight in a single dose(the total dosage of Tinidazole was not over 2 grams for one person). All cases(38) showed negative conversion of Giardia cyst in their feces 4 and 8 days after treatment but 14 days after drug administration, 3(7.9%)out of 38 cases showed Giardia cysts in their stool examinations. The tolerance of this drug was good, and the side effects were noted mild and transient.

      • 물달팽이科 (Family Lymnaeidae)의 애기물달팽이 및 물달팽이에 있어 巨大肝蛭에 對한 感受性과 同位酵素에 關한 硏究

        李康伊,金鍾煥 충남대학교 자연과학연구소 1993 忠南科學硏究誌 Vol.20 No.2

        The present studies were carried out to examine the susceptibility and the infection with miracidia of a liver fluke, Fasciola gigantica and to analyze isozymes in snails, L. pervia and L.(R.) auricularia of Korean strains. The snails were collected from 213 localities in Korea. The distributive status and the type of habitats of the snails were studied, and cercariae, naturally shed from the snails were observed. For the morphological identification of cercaria, 0.1% brilliant cresyl blue stain was employed. The buffer systems used in electrophoresis were the continuous buffer system of Tris citrate gel(pH 8.0) and the discontinuous buffer system of Poulik gel(pH 8.7). 11 isozymes were demonstrated on Tris citrate gel such as adenylate kinase(AK), adenosine deaminase(ADA), α-glycerophosphate dehydrogenase(α-GPD), peptidase(Pept), xanthine dehydrogenase(XDH), sorbitol dehydrogenase(SDH),phosphoglucomutase(PGM), malate dehydrogenase(MDH), isocitrate dehydrogenase(IDH), glutamate oxaloacetate transaminase(GOT) and mannose phosphate isomerase(MPI), and 4 isozymes were demonstrate on Poulik gel ; Phosphoglucose isomerase(PGI), Peroxidase(Perox), esterase(ES) and general protein(GP). The results obtained in the present studies are summarized as follows ; 1. L. pervia and L. auricularia were collected from 78(36.6%) and 113(53.1%) out of 213 localities surveyed, respectively. It was suggested that the latter is more widely distributed over the country. The snails of both species were found in only 19 localities. 2. L. pervia were mainly detected in the districts along the Yeonsan, the Imjin the Han, and the Geum riversides and L. auricularia were found more commonly along the Han, the Geum, the Imjin, and the Gagog riversides. Parsley fields, ponds and rice paddy fields were common habitats of L. pervia. The both snails were detected commonly in parsley fields and ponds. 3. A total of 7 kinds of cercariae were naturally shed from the both snails, L. pervia and L.(R.) auricularia; Fasciola spp. Echinostoma spp., two types of furcocercous cercariae and one xiphidiocercaria were emerged from the both snails but Plagiorchis muris was from the former and Cercarial ellipsoidae was from the latter, respectively. More than one kind of cercaria has never been shed from one individual of snails. 4. L. pervia infected experimentally with 10 miracidia of F. gigantica revealed the infection rates of 53.1% in the 5∼7days old group, 60.0% in 15∼17days old group and 40.5% in 60 days old (adult snail) group after hatching the snails, while L.(R.) auriculria showed the significantly lower infection rates (p<0.005) than those of L. pervia. The infection rates were 13.9% in the 5∼7 days old group and 4.7% in 15∼17 days group. No any snail infected was encounted in the 60 days group of the snail. These figures strongly indicated that L. pervia is the intermediate host of F. gigantica in Korea. 5. L. pervia and L.(R.) auricularia in the electrophorectic study on isozyme, revealed one band in ADA, α-GPD, XDH, SDH, PGM, MPI and PGI, 2 bands in GOT, MDH, IDH and Perox, 3 in AK and Pept, 7 in ES, and general protein was showed 4 bands, respectively. On the other hand, the study of Perox isozyme was showed 1 band in L.(R.) auricularia and 2 band in L. pervia. 6. On the basis of the electrophoretic patterns of AK, ADA, Pept, SDH, PGM, IDH, Perox and ES and GP which shown the distinctive differences in mobility, the species specificity was noticed in L. pervia and L.(R.) auricularia. The electrophoretic patterns of α-GPD, XDH, MDH, GOT, MPI and PGI were the same electrophoretic mobilities in the both snails. However, it was strongly suggested that an intensive study on isozyme with L. pervia and L.(R.) auricularia remains to be carried out as far as the taxonomy of the both snails is concerned.

      • KCI등재후보

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