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한약재 혼합추출물이 생쥐 탈모 모델의 모발성장 촉진에 미치는 영향
황미열 ( Mee Yul Hwang ),도은주 ( Eun Ju Do ),정금희 ( Geum Hee Jung ),김미려 ( Mi Ryeo Kim ) 한약응용학회 2014 한약응용학회지 Vol.14 No.2
Objective:This study was performed to examine the effect of mixed medicinal herbal extract including Lespedeza bicolor Turcz. and 18 others (CS-10-H) on hair growth in an alopecia model of C57BL/6 mice. Methods:Male mice were divided into 3 experimental groups for treatment of CS-10-H or 5% minoxidil or vehicle solution, respectively. Dorsal skin of animals were shaved and topically applied with an amount of 200 μL of test materials once a day for 3 weeks. The apparent hair growth was monitored at 5, 10, 15 and 21 days after topical application. The changes of hair density and hair thickness were evaluated by phototrichogram using folliscope. Protein expression levels of hair growth-related factors were measured by western blotting. Results:Application of minoxidil or CS-10-H stimulated the hair growth compared to vehicle treatment. Hair density of minoxidil and CS-10-H group was increased about 206% and 208% on 14 day compared to that of vehicle group, respectively. Hair thickness of minoxidil and CS-10-H group was also increased about 220 % and 200% on 14 day compared to that of vehicle group. The protein expression of hair growth factors, IGF-1 and VEGF was significantly up-regulated in CS-10-H and minoxidil group than in vehicle treated group. Conclusion:These data suggest that CS-10-H shows potential usefulness as ingredients of hair tonic and hair-restorer for development of cosmetics and quasi-drugs, as result of its potent stimulating activity on hair growth in C57BL/6 alopecia model.
경옥고가미방 추출물이 생쥐의 양모 및 발모 관련 단백 발현에 미치는 영향
도은주 ( Eun Ju Do ),황미열 ( Mee Yul Hwang ),김승연 ( Seung Yeon Kim ),이진상 ( Jin Sang Lee ),양대석 ( Dae Seok Yang ),양재하 ( Chae Ha Yang ),김미려 ( Mi Ryeo Kim ) 대한본초학회 2011 大韓本草學會誌 Vol.26 No.4
Objective: Present study was carried out to investigate the effect of Gyungohkgo-gamibang extract on hair growth and protein expression in an alopecia model of C57BL/6 mice. Methods: Mice were divided into 3 experimental groups including normal (vehicle), Gyungohkgo-gamibang extract (YNS-10) and 5% minoxidil-treated group. The test materials were daily applied with 0.1 ml per mouse on shaved dorsal skin for 3 weeks. The hair growth was monitored by photograph at 5, 10, 15, 21 days after topical application. Then the changes of hair density and hair thickness in the hair-removed area were evaluated by phototrichogram using folliscope. Also the expression level of growth factors related to hair growth was measured by western blotting. Results: Application of minoxidil or YNS-10 stimulated the hair growth compared to vehicle treatment. Therefore hair density of minoxidil or YNS-10 application was increased about 200% and 210% more than in vehicle application on 14 day, respectively. And hair thickness of both minoxidil group and YNS-10 group was increased about 220% and 210 % more than in vehicle spreading on 14 day, respectively. Futhermore the protein expression of IGF-1 and VEGF were significantly up-regulated on 7 day in YNS-10 and minoxidil-spreaded group compared to vehicle-applied group. Conclusion: These data suggest that YNS-10 has potent stimulating activity on hair growth in C57BL/6 mice and potential usefulness as ingredients of hair tonic and hairrestore.
콘택트렌즈 보존 용기 유래 Acanthamoeba lugdunensis KA/L5주의 내공생세균
정동일,공현희,김태호,황미열,유학선,윤호철,설성용 경북대학교 의학연구소 1999 경북대학교병원의학연구소논문집 Vol.3 No.1
콘택트렌즈 보존 용기 유래 가시아메바 KA/L5주의 세포질 내에 존재하는 bacterial endosymbiont(내공생세균)를 투과전자현미경으로 관찰하여 확인하였다. 숙주인 가시아메바 KA/L5주는 형태학적으로 제2군에 속하였고, rDNA PCR-RFLP 결과 A. lugdunensis로 동정되었다. 미토콘드리아 DNA RFLP와 동위효소 분석상 이 충주는 국내 콘택트렌즈 보존용기에서 가장흔히 분리되는 type인 KA/Ll주, 국내 임상 분리주 중 하나인 KA/E2주, 내공생세균을 가지는 것으로 보고된 병원 냉각수 유래 KA/W4주 및 L3a주와 동일하거나 매우 유사한 성적을 보였다. 내공생세균은 약 1.38× 0.507㎛의 크기였고, 아메바 세포질 내에 불규칙하게 분포하고 있었으며. 그 표면에 아메바의 ribosome이 부착되어 있었다. 내공생세균을 둘러싼 lacunae나 막과 같은 구조는 관찰되지 않았다. Legionella 특이 primer를 이용한 효소중합반응(PCR)에서 내공생세균의 염색체 DNA는 증폭되지 않았다. A. lugdunensis의 우리말 이름을 담수가시아메바로 제안한다. Transmission electron microscopy of an Acanthamieba isolate (KA/L5) from a contact lens case revealed bacterial endosymbionts within cytoplasm of the amoebae, The Acanthamoeba isolate belonged to the morphological group II. Based on the polymerase chain reaction (PCR) - restriction fragment length Polymorphism (RFLP) of 18S ribosomal RNA coding DNA (rDNA), the Isolate was identified as A. lugdunensis. Strain typing by isoenzyme analysis using isoelectric focusing (IEF) and mitochondrial (Mt) DNA RFLP revealed that the isolate was closely related with KA/Ll , the most predominant type of isolates from contact lens storage cases, KA/E2, a clinical isolate, KA/W4, preciously reported to host endosymbionts, and L3a strains of A. lugdunensis. The endosymbionts were similar to those of KA/W4 In aspects that they were randomly distributed in both trophozoites and cysts, and were rod-shaped bacteria measuring approximately 1.38x 0.50㎛. But the number of endosymbionts per amoeba was significantly lower than that of KA/W4. They were neither limited by phagosomal membranes nor included In lacunae- like structure.