가축축사와 양조장에서 야생효모의 분리와 추출조건

홍창일,윤선영,김진환,이지학,서영란,원혜민,정의길,한국일,김선기,한만덕 순천향대학교 기초과학연구소 2017 순천향자연과학연구 논문집 Vol.23 No.2

Yeast extract is used as food and animal additives. The study was conducted the selection and isolation of wild type yeast with excellent autolysis to produce nutritious yeast extract and to use with prebiotics. A number of wild yeasts were isolated from Korean domestic stable, brewery, and food. Isolated yeasts were identified by comparison of nucleotide sequences for polymerase chain reaction-amplified ITS1/4 region of 18S rRNA using BLAST. In order to investigate the optimal autolysis conditions, the selected strains were autolysed by several methods (e.g. ethanol, autoclaving, homogenizing, deep freezing, NaCl, NaOH, nitrogen, protease, ultrasonicating, and pH). The efficiency of yeast cell autolysis was evaluated with contents of β-glucan and mannan in autolysates. Sixty-four yeast strains were isolated from 148 samples from yeast selection medium, yeast-mold agar (YMA) supplemented with 10% ethanol, and thirteen yeast species were identified by BLAST. Most stain of thirteen yeast were autolyzed about 65% by autoclave condition (121℃). S. cerevisiae strain P1 showed 31% of autolysis at pH 4.4 condition for 72 hrs. The results suggest that the newly isolated wild-type yeast and the newly developed hydrolysis conditions can be used in the development of animal feed additives.

인접 잉여 피부를 이용한 아랫 눈꺼풀 결손의 재건

홍창일,김선구,김유진,이세일,Hong, Chang-Yil,Kim, Sun-Goo,Kim, Yu-Jin,Lee, Se-Il 대한성형외과학회 2010 Archives of Plastic Surgery Vol.37 No.4

Purpose: Facial tumor excision is a common cause of lower eyelid defect in old patients. Many methods have been introduced for the reconstruction of lower eyelid. However, conventional surgical method can cause various complications like scar, ectropion and unnatural color matching. Thus, we introduce a simple and aesthetically acceptable method for the reconstruction of lower eyelid defect. Methods: Three elderly patients with skin cancer in the unilateral lower eyelid were operated by the new method. Following a wide excision of skin cancer, subcilliary incision of lower blepharoplasty was carried out. Elevated skin flap of lower eyelid was redrapped for the correction of defect and the remnant skin from lateral portion of lower eyelid was used for full thickness skin graft (FTSG) to correct the remaining defect. Results: All grafts survived and color match of the graft was excellent without ectropion. Furthermore, wrinkles of the lower eyelid were improved after the blepharoplasty. Conclusion: Lower eyelid defect resulting from wide excision of malignant tumor in old patients could be reconstructed successfully by modifying the conventional lower eyelid blepharoplasty along with FTSG using the remnant skin.

Streptococcus dysgalactiae로부터 분리된 히알루론산과 황화된 유도체의 구조와 항염증 활성

홍창일(Chang-Il Hong),정의길(Eui-Gil Jung),한국일(Kook-Il Han),김용현(Yong Hyun Kim),이성희(Sung Hee Lee),이홍섭(Hong Sub Lee),한만덕(Man-Deuk Han) 한국생명과학회 2016 생명과학회지 Vol.26 No.5

히알루론산(HA, Hyaluronic acid)은 β-1, 3-N-acetyl glucosamine과 β-1, 4-glucuronic acid가 반복된 선형 폴리머 고분자로서 생물학적 활성 및 생체친화성 특성 때문에 의약 및 약학분야에서 중요한 분자로 여겨지고 있다. 본 연구는 HA을 S. dysgalactiae으로 얻고, 화학적 방법을 통해 황화된 히알루론산(S-HA, Sulfated hyaluronic acid)유도체를 합성하여 그 구조와 항염증 활성을 비교하였다. HA의 생산은 S. dysgalactiae를 5 l 생물반응기를 이용하여 대량 배양하여 수용성 히알루론산(HA-WS, water soluble hyaluronic acid)과 비수용성 히알루론산(HA-WI, water insoluble hyaluronic acid)을 분리 정제하였다. 특히 HA-WI를 황화시켜 황화된 히알루론산(S-HA) 유도체를 합성하였으며, 그 수율은 90%로 나타났다. 합성된 S-HA의 구조를 FT-IR 및 ¹H/<SUP>13</SUP>C-NMR를 통해 S. dysgalactiae 로부터 생산된 표준 HA, HA-WS 및 HA-WI와 비교 분석한 결과, 황으로 치환된 양상을 확인하였다. 또한, S-HA의 항염증 활성을 RAW 264.7 대식세포를 통해 확인한 결과, S-HA는 천연 형태의 HA (HA, HA-WS)보다 nitric oxide (NO)와 COX-2 및 PGE₂ 유전자 발현이 유의하게 낮게 발현되었다. 염증 매개 cytokine인 TNF-α (<80 pg/ml) 및 IL-6 (<100 pg/ml)의 생성도 S-HA가 천연 HA보다 낮은 수준으로 정량되었다. 이 같은 결과에서 황화된 S-HA은 천연 히알루론산보다 용해성이 우수하고 염증관련 사이토카인의 생성 억제를 통해 항염증 효과를 나타내므로 염증치료제, 성형 및 생체 적용 약물전달 소재로 그 활용이 기대된다. Hyaluronic acid (HA) is an important macromolecule in medical and pharmaceutical fields. HA is a natural and linear polymer composed of repeating disaccharide units of β-1, 3-N-acetyl glucosamine and β-1, 4-glucuronic acid. This work aimed to confirm the structural characteristics and anti-inflammatory activities of HA and its chemically sulfated-HA. HA was produced from a fed-batch fermentation process using Streptococcus dysgalactiae in a 5 l bioreactor. HA was isolated water-soluble form (HA-WS) and water-insoluble form (HA-WI) from culture medium, and was obtained chemically sulfated-derivative (S-HA) that resulted in a 90% yield from HA-WI. The structural features of the sulfated- HA (S-HA) were investigated by FT-IR and ¹H-NMR spectroscopy. The FT-IR and NMR patterns revealed the similarity in both the FTIR spectrum as well as NMR spectrum of both reference standard and purified HA from S. dysgalactiae. The anti-inflammatory activities of HA and S-HA were examined on LPS-induced RAW 264.7 cells. S-HA was significantly inhibited production of pro-inflammatory mediators such as nitric oxide (NO) and PGE₂ and the gene levels of iNOS and COX-2, which are responsible for the production of NO and PGE₂, respectively. Furthermore, S-HA also suppressed the overproduction of pro-inflammatory cytokine TNF-α (<80 pg/ml) and IL-6 (<100 pg/ml) compared to that of HA-WI. The present study clearly demonstrates that HA-S exhibits anti-inflammatory activities in RAW 264.7 macrophage cells.

전력계통 구조물의 상태진단용 자가발전 무선 센서 노드 개발 및 평가

김창일 ( Chang Il Kim ),정영훈 ( Young-hun Jeong ),윤지선 ( Ji Sun Yun ),홍연우 ( Youn Woo Hong ),장용호 ( Yong-ho Jang ),최범진 ( Beom-jin Choi ),박신서 ( Shin-seo Park ),손천명 ( Chun Myung Son ),서덕기 ( Duck Ki Seo ),백종후 ( 한국센서학회 2016 센서학회지 Vol.25 No.5

A self-powered piezoelectric energy harvester was developed for the application in wireless sensor node. The energy harvester was evaluated with power generation characteristics for the wireless sensor node for structural diagnosis of the electric power system. The self-powered wireless sensor node was set to measure temperature, vibration frequency of the electric power system. A piezoelectric harvester composed of 7 uni-morph cantilevers (functionalized as 6 generators and 1 vibration sensor) was connected to be an array and revealed to produce significantly high output power of approximately 10 mW at 120 Hz under 3.4 g((1 g = 9.8 m/sec²). The wireless sensor node could work as the electric power generated by the developed piezoelectric harvester.

연구논문 - 유기 / 정밀 : Oxidation 에 의한 Carbon Black 의 물리 , 화학적 성질의 변화 및 분산성에 관한 연구

이창일,이창우,홍영호,장윤호,홍인권,함영민 ( Chang Il Lee,Chang Woo Lee,Young Ho Hong,Yun Ho Jang,In Kwon Hong,Yeong Min Hahm ) 한국공업화학회 1995 한국공업화학회 연구논문 초록집 Vol.1990 No.3

진동 각도에 따른 유니몰프 압전 캔틸레버의 발전특성연구

김창일 ( Chang Il Kim ),윤지선 ( Ji Sun Yun ),박운익 ( Woon-ik Park ),정영훈 ( Young-hun Jeong ),홍연우 ( Youn Woo Hong ),조정호 ( Jeong-ho Cho ),백종후 ( Jong Hoo Paik ) 한국센서학회 2017 센서학회지 Vol.26 No.2

Energy source of a piezo-electric harvester is vibration. Sources of vibration are machineries operated with high frequencies, constructions and people operated with low frequencies and etc. In this study, we tried to figure out power generation properties over vibrations upon angles of a piezo-cantilever for applying them to movements of the construction and/or people, which are vibration sources at low frequencies. A uni-morph cantilever with a 59 mm × 29 mm × 0.2 mm piezo-electric element attached on a 71 mm × 46 mm × 0.25 mm copperplate was used. A spring was attached to the lower side of the cantilever and a mass was attached on the opposite side. Also, a structure with a specific angle which is an angle in between the ground and the cantilever was prepared and then, connected to a spring or the cantilever. Then, this structure was divided into the A-type and B-type and excited in the direction of z- axis. After that, the angle between the ground and the cantilever was changed and excited by 1 to 10 Hz upon the existence of a spring and/or a mass to compare power generation properties.

벤더형 모듈을 이용한 광대역 압전 하베스터 연구

김창일,권태형,여서영,정영훈,홍연우,조정호,백종후,윤지선 한국센서학회 2018 센서학회지 Vol.27 No.2

In this study, a bender-type piezoelectric energy harvester was fabricated and evaluated to compensate for the dis- advantages of high-power generation only in the resonance frequency range of a piezoelectric harvester using a piezo- electric cantilever. The generated power was investigated according to various changes in the vibration environment. Compared with the piezoelectric cantilever module, the bender-type piezoelectric module showed a larger number of peak voltages. The primary peak voltage shifted toward the low frequency when the spring was coupled to the bender-type piezoelectric module. The harvester of the three bender-type modules had a vibration frequency exceeding 1 mW in the 34-45 Hz range and generated 3.112 mW of power at the vibration frequency of 38 Hz. The harvester of the six bender- type modules had a vibration frequency exceeding 1 mW in the 31-45 Hz range and generated 3.081 mW of power at the vibration frequency of 35 Hz.

노인 운동프로그램 참여가 성공적 노화에 미치는 영향 : 생활양식 변화의 매개효과를 중심으로

이창일,홍경주,이원웅 한국통합사례관리학회 2014 한국케어매니지먼트연구 Vol.13 No.-

본 연구는 노인 운동프로그램이 생활양식 변화와 성공적인 노화에 미치는 영향을 살펴보며, 노인 운동프로그램 개발의 기초자료를 제공하기 위한 목적으로 수행되었다. 본 연구는서울 및 수도권에 거주하는 60세 이상 노인 600명을 대상으로 2014년 4월 16일∼5월 3일에 설문조사를 실시하였으며, 이 중 566부를 최종 분석으로 사용하였다. 연구결과를 살펴보면 첫째, 배우자가 있는 경우, 학력이 높은 경우, 종교가 있는 경우, 매우 건강한 경우운동프로그램의 참여가 높았다. 둘째, 나이가 어린 경우, 학력이 높은 경우, 경제수준이 높은 경우, 유배우자의 경우, 경제활동을 하는 경우, 종교가 있는 경우, 주관적 건강수준이 높은 경우 생활양식 변화와 성공적노화가 높았다. 셋째, 운동프로그램에 참여하거나 참여기간, 참여빈도, 참여시간이 높은 경우 생활양식 변화와 성공적 노화가 높았다. 넷째, 운동프로그램 참여가 성공적 노화에 미치는 영향에서 생활양식변화는 부분매개효과가 성립됨을 알 수 있었다. 결론적으로 운동프로그램은 신체적 기능을 높게 유지할 수 있으며,또한 운동이나 규칙적인 생활, 적절한 식습관 등의 생활양식 변화를 통해 성공적 노화를 향상시킬 수 있다. This study was conducted to examine the effects of senior exercise program on successfulaging and lifestyle changes. The subjects of this study were aged 60 and older living in Seouland metropolitan area and survey was conducted April 16, 2014 to May 3, 2014. The 600questionnaires were distributed and among them 596 questionnaires were finally collected. 566questionnaires were used in the final analysis. The results are as fellows;First, The participation of senior exercise program was higher in people having spouse,higher education, good health. Second, successful aging and lifestyle changes were was higherin younger age, higher education, higher economic levels, economic activity, having religion,the higher level of subjective health. Third, successful aging and lifestyle changes were higherin the case of participating in an exercise program and higher participating period, rate, time ofexercise program. Fourth, lifestyle changes had mediating effect between participation in seniorexercise program and successful aging. The findings of this study imply that senior exerciseprogram can maintain high physical function, successful aging can be improved through lifestylechanges, such as proper eating habits, exercise and regular life.

방전 플라즈마 소결법에 의한 ZnS 나노입자의 소결과 광학적 특성

김창일,김유비,여서영,홍연우,윤지선,박운익,정영훈,조정호,백종후,Kim, Chang-Il,Kim, You-Bi,Yeo, Seo-Yeong,Hong, Youn-Woo,Yun, Ji-Sun,Park, Woon-Ik,Jeong, Young-Hun,Cho, Jeong-Ho,Paik, Jong-Hoo 한국전기전자재료학회 2017 전기전자재료학회논문지 Vol.30 No.6

Zinc sulphide (ZnS) nanoparticles were fabricated by hydrothermal synthesis at $180^{\circ}C$ for 12 h. Two kinds of ZnS powder (hydrothermal synthesized ZnS and commercial ZnS) were investigated by X-ray diffraction (XRD) and scanning electron microscopy (SEM) for phase and microstructure, respectively. The XRD patterns showed that all ZnS nanoparticles have a sphalerite (cubic) structure. The nanoparticles of two different ZnS powders were sintered by spark plasma sintering. The sintered ZnS were analyzed by XRD, SEM, and FT-IR. We found that the transmittance of the infrared region is highly dependent on the density and crystal structure of sintered ZnS and the purity of the starting ZnS powder.

표면처리에 의한 Carbon Black 의 표면물성변화

이창우,이창일,홍영호,홍인권,장윤호,함영민 ( Chang Woo Lee,Chang Il Lee,Young Ho Hong,In Kwon Hong,Yun Ho Jang,Yeong Min Hahm ) 한국공업화학회 1996 공업화학 Vol.7 No.4

카본블랙은 일반적으로 보강성, 착색성, 내후성, 내화학성 및 전기적 전도성 등의 다양한 특성을 가지고 있으나 매질과의 친화력이 낮아 이용하는데 어려움을 내포하고 있다. 따라서 본 연구에서는 카본블랙의 표면특성을 변화시켜 매질과의 친화력을 향상시키기 위하여 표면에 비교적 많은 기능기를 가지고 있는 channel black은 표면 카르복실기의 수소를 알칼리 금속과 치환하였고, 표면 기능기가 거의 존재하지 않는 furnace black은 질산으로 처리하였다. 이와 같이 표면의 특성을 변화시켜 카본블랙의 물성변화를 측정하고, 수용액 속에서의 분산특성을 연구하여 다음과 같은 결론을 얻을 수 있었다. 표면처리된 channel black의 경우 치환된 금속(Li, Na, K)의 이온반경이 증가함에 따라 입자 표면의 수화도 증가로 인하여 분산성이 향상되었고, 질산처리한 furnace black의 경우 산의 농도 및 반응온도에 비례하여 표면 기능기의 증대를 가져왔으며 반응시간에는 크게 영향을 받지 않음을 알 수 있었다. 또한 전해질 용액에서 pH 변화에 따른 제타전위를 측정한 결과 카본블랙 입자의 표면 전하밀도가 증가하고, 이에 따른 전기적 반발력 증가로 인한 분산성의 향상과 비교적 안정한 계를 이루고 있음을 확인하였다. Carbon Black has various advantageous properties such as reinforcement, tinting, weathering, chemical resistance, electrical resistance, etc., but it has poor affinity in the solution. The purpose of this study is to enhance dispersion in the aqueous solution by altering the surface characteristics. Two different treatments were applied for both channel black and furnace black respectively. Channel black was treated in alkaline solution to replace hydrogen of the carboxyl group with alkaline metal, Li, Na, K. Furnace black has few functional groups, and it was treated with HNO₃to increase the number of functional group on carbon black. It was seen that the substitution of alkaline metal on the surface of channel black enhances the degree of dispersion. Also the higher the acid concentration and reaction temperature, the more the number of some functional groups on the furnace black. Reaction time was not seen to be effective.