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HaCaT 세포주에서 Epigallocatechin-3-Gallate (EGCG)가 자외선 및 LPS에 의한 iNOS mRNA 발현 및 NO 생성에 미치는 영향
탁우정 ( Tag U Jeong ),이창균 ( Lee Chang Gyun ),서성준 ( Seo Seong Jun ),김명남 ( Kim Myeong Nam ),노병인 ( No Byeong In ),홍창권 ( Hong Chang Gwon ) 대한피부과학회 2004 대한피부과학회지 Vol.42 No.1
N/A Background: Nitric oxide (NO) plays an important role in inflammation and multiple stages of carcinogenesis. Green tea (Camellia sinensis polyphenols are potent antiinflammatory agents and have been shown to inhibit NO production in tumor cell lines. In the present study, we examined the effect of epigallocatechinp-3-gallate (EGCG), a generation of NO in HaCaT cells. Methods: HaCaT cells were treated with 10μM EGCG and 100μM NAC for 1 hour. 1 hour later, they were irradiated with 50mJ/cm^2 UVB and treated with 200 u㎍/ml LPS for 12 hours, respectively. The iNOS mRNA was determined by reverse transcription-polymerase chain reaction (RT-PCR) and NO production was assessed by spectrophotometric method based on Griess reaction. Nuclear factor κB (NF-κB) binding activity was determined by electophoretic mobility shift assay (EMSA). Results: The results were as follows 1. EGCG inhibited UVB and LPS induced expression of inducible nitric oxide synthase. 2. HaCaT cells cotreated with EGCG produced significantly less iNOS mRNA and NO compared with HaCaT cells stimulated with UVB irradiation of LPS. 3. The inhibition of iNOS mRNA and NO production correlated with the suppression of expression of NF-κB dependent gene iNOS. 4. EGCG inhibited the activation and translocation of NF-κB to the nucleus Conclusion: inhibited the activation and translocation of NF-κB to the nucleus in HaCaT cells by interfering with the activation of NF-κB through a novel mechanism. Our results further suggest that EGCG may be therapeutically effective in UVB and cytokine induced cutaneous inflammation. (Korean J Dermatol 2004;42(1):37~46)
최성욱 ( Choe Seong Ug ),서성준 ( Seo Seong Jun ),김명남 ( Kim Myeong Nam ),홍창권 ( Hong Chang Gwon ),노병인 ( No Byeong In ),송계용 ( Song Gye Yong ) 대한피부과학회 2003 대한피부과학회지 Vol.41 No.11
Scrofuloderma results from a direct extension of an underlying tuberculosis focus such as the lymph node, bone or joint to the underlying skin, and is often associated with pulmonary tuberculosis. One 72-year-old man presented with erythematous swollen patch with scales on the left hand and erythematous swollen patch on the left elbow. He had tuberculous osteomyelitis of left elbow. And another patient who is a 59-year-old man presented with hemorrhagic, purulent ulcers with crust and exudate on the left and right chest. Each size of ulcers are 6.0×5.Ocm and 5.O×5.Ocm. He had reactive pulmonary tuberculosis and tuberculous spondylitis. Biopsy specimens from the skin showed lymphocytes, epitheloid cells, and Langerhan`s giant cells with caseation necrosis. Acid-fast bacilli were identified on AFB staining. We report two unusual cases of scrofuloderma associated with tuberculous osteomyelitis and pulmonary tuberculosis respectively. (Korean J Dermatol 2003;41(11) : 1503∼1507)
백반증 환자의 자가항체 검출에 따른 임상양상에 대한 고찰
김범준 ( Kim Beom Jun ),서성준 ( Seo Seong Jun ),홍창권 ( Hong Chang Gwon ) 대한피부과학회 2003 大韓皮膚科學會誌 Vol.41 No.11
N/A Background : Vitiligo has been associated with various disorders including pernicious anemia, diabetes, hyperthyroidism, hypothyroidism, alopecia areata, Addison`s disease, and so on. Autoantibodies against specific organs are also frequently found. However, the positive rates of these autoantibodies` detection by various reporters showed too much diversity to extrapolate a definitive conclusion. Objective : The purpose of this study was to investigate the autoimmune aspects of vitiligo by evaluating the detection rates of various organ-specific autoantibodies. Methods : We classified vitiligo patients into 2 groups(autoantibody positive group and autoantibody negative group) and 3 types(localized, generalized, and universalis). The overall detection rates of various autoimmune and endocrine diseases were also assessed in patients and the control, which was composed of 40 young healthy volunteers. Results : 1. There were 106 males(33.0%) and 215 females(67.0%) in total 321 vitiligo patients. 2. 115 patients(35.8%) were determined to be autoanitibody positive, and 206 patients(64.2%) were negative. 3. The most frequent type of vitiligo was generalized type in both autoantibody positive and negative group. 4. Mean age of onset was 30.8 years. And there was a statistically significant difference between autoantibody positive(37.6 years) and negative(29.3 years) group(p < 0.05). 5. Average disease duration was 6.5 years in autoantibody positive group and 4.3 years in autoantibody negative group. 6. The positive rates of anti-nuclear, anti-microsomal. anti-smooth muscle antibody, and rheumatoid factor showed no significant differences between vitiligo patients and normal control group(p > 0.05). But the positive rate of antithyroid antibody was significantly higher in vitiligo patients than in normal control(k²= 4.234, p = 0.040). 7. The prevalence rates of autoimmune & endocrine disorders showed no significant differences between vitiligo patients and normal control(p > 0.05). 8. The prevalence rates of autoimmune and endocrine disorders were higher in autoantibody positive vitiligo group(33.0%) than in negative group(29.6%), although it was not statistically significant(p > 0.05). However, the prevalence rate of thyroid disease itself was significantly higher in autoantibody positive group than in negative group(p = 0.004). 9. Treatment response to PUVA therapy was better in autoantibody positive group than in negative group(p = 0.0003). Conclusion : With these results, we were able to conclude that anti-thyroglobulin antibody is more frequently found in vitiligo patients than in normal control. And autoantibody positive vitiligo patients showed higher prevalence rate of thyroid disease and better treatment response to PUVA therapy than in autoantibody negative vitiligo patients. Therefore, the presence of autoantibody in vitiligo patients seems to be related with a better treatment response for PUVA therapy. (Korean J Dermatol 2003;41(11) : 1463∼1470)
최한규,홍창권,심주현,서성준 대한피부과학회 2000 대한피부과학회지 Vol.38 No.8
Propylene glycol, which is capable of producing both primary irritant and allergic skin reaction, is a common used vehicle for numerous topical preparations. The topical imidazole antimycotics are widely used but an infrequent cause of contact allergy. A 20-year-old female presented with erythematous scaly patch on the lower leg. An erythematous pruritic papulovesicular eruption developed around the lesion after applying Nizoral cream and was aggravated after applying Travogen cream. Patch test with Korean standard series and the ingredients of both cream revealed positive reaction to the propylene glycol contained in Nizoral cream and isoconazole nitrate in Travogen cream.