http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 홍수종 (검색결과 279 건)
- 무료
- 기관 내 무료
- 유료
-
-
천식 환자에서 분리된 Dermatophagoides pteronyssinus 항원 - 특이 T세포 클론의 특성
홍수종,오순환,조영주,유빈,고윤석,문희범 대한천식알레르기학회 1995 천식 및 알레르기 Vol.15 No.1
T cells play a central role in IgE synthesis and airway inflammation in asthmatic patients. In order to characterize antigen specific T cell clones, we established Dermatophagoides pteronyssinus(D.p) specific and purified protein de- rivatives(PPD) -specific T cell clones, and investigated their cytokine production profile in response to the antigenic and nonspecific stimulation. We established six D.p-specific T cell clones from the peripheral blood of two atopic asthmatics and seven PPD-specific T cell clones from one PPD sensitized ayrnptomatic individual and two pulmonary tuberculosis pa- tients using limiting dilution method. We analyzed the ability of T cell clones to produce IL 4, IFNy and their IL 2R expres- sion upon stimulation with D.p, PPD or PHA plus TPA. Only one D.p-specific T cell clone produced IL 4 upon stimulation with D.p, whereas three clones produced both IL 4 and IFNy upon stimulation with PHA plus TPA, Four PPD specific T cell clones produced IFNy upon stimulation with PPD, five clones produced IFNy upon stimulation with PHA plus TPA, but none of them produced IL-4 regardless of stimulation. Two of five D.p-specific T cell clones and one of four PPD-specific T cell clones showed more than 200 % increass in IL 2R expression upon stimulation with each antigen. These data showed that some of D.p-specific T cell clones were able to produce both IL 4 and IFNy after nonspecific stimulation, but PPD specific T cell clones produced only IFNy regardless of stimulation. This suggests that D. p-specific T cell clones have the ability to produce TH, type cytokines depending on the stimulation.
-
-
천식환아와 정상아에서 β2-아드레날린 수용체 유전자 다형에 관한 연구
홍수종,김봉성,김자형,오홍범,이무송,최수옥,김미영,오선영,박양,고영률 대한 소아알레르기 호흡기학회 2002 Allergy Asthma & Respiratory Disease Vol.12 No.4
목 적 : 천식의 치료에 있어서 염증조절제의 역할이 강조되고 있기는 하지만, 아직 β2-아드레날린 수용체와 반응하여 기도 수축을 이완시키는 β2-항진제의 역할은 중요하다. 근래에 β2-아드레날린 수용체 유전자 다형과 관련하여, Gly16과 중증 천식 및 야간 천식과 관련이 있고, Glu27은 천식에서 기도과민성의 감소와 관련이 있으며, Gly16 Gln27 일배체형은 아토피군에서 야간 기침과 관련이 있다고 보고되었다. 본 연구에서 천식환자와 정상군에서 β2-아드레날린 수용체 유전자 다형이 차이가 있는지 확인하고, β2-아드레날린 수용체 유전자가 아토피지표 및 기도과민성과 연관성이 있는지 조사하였다.방 법 : 천식으로 진단된 99명과 아토피가 없는 정상아 73명을 대상으로, 말초혈액내 총 호산구수와 혈청 총 IgE를 측정하였고, 메타콜린 기관지 유발검사 및 폐기능검사를 실시하였다. β2-아드레날린 수용체 유전자의 일배체형 분석을 위해 아미노산 16과 27의 부위를 포함하고 있는 β2-아드레날린 수용체의 유전자부위를 polymerase chain reaction(PCR)으로 증폭한 후 16번 유전형은 allele specific PCR, 27번 유전형은 PCR-restriction fragment length polymorphism(RFLP) 방법으로 분석하였다.결 과 : β2-아드레날린 수용체의 16번과 27번 대립 유전자의 빈도는 두 군 사이에 차이가 없었으며, 16번과 27번 아미노산의 일배체형 빈도도 두 군 사이에 차이가 없었다. 그러나 Arg16 Glu27은 한 례도 없어 Arg16은 Gln27과 강한 연쇄불평형이 관찰되었다. 천식군에서 총호산구수와 호산구 분율(%) 및 총 IgE 농도는 각각의 일배체형에 따라 차이가 없었으며, PC20 및 기도 자극 후 β2-항진제에 대한 반응 수치도 각각 일배체형에 따라 차이가 없었다. β2-adrenoceptor Polymorphisms between Asthmatic and Normal Children in KoreaSoo-Jong Hong, M.D.1, Bong-Seong Kim, M.D.1, Ja-Hung Kim, M.D.1,Heung-Bum Oh, M.D.2, Moo-Song Lee, M.D.3, Soo-Ok Choi, M.D.4,Mi-Young Kim, M.D.4, Sun-Young Oh, M.D.5, Yang Park, M.D.6, Young-Yull Koh, M.D.6
-
홍수종,안강모,이수영,김규언 대한 소아알레르기 호흡기학회 2008 Allergy Asthma & Respiratory Disease Vol.18 No.1
Asthma and allergic diseases are one of the most common disorders in children. Due to its increased prevalence, as well as the increased morbidity and mortality from these diseases, asthma and allergic diseases have come to be recognized as a major worldwide public health issue. In addition, socioeconomic burden of asthma and allergic diseases has increased in Korea also. The International Study of Asthma and Allergies in Childhood (ISAAC) investigated the worldwide prevalence of asthma and other allergic diseases using simple standardized methods because of the comparison of asthma and allergic diseases between the countries. In Korea, several epidemiologic studies have been conducted to determine the prevalence of asthma in children. Although these studies showed increased prevalence of asthma among Korean children (from 3.4% in 1964 to 10.1% in 1989), these findings were based on data from small numbers of subjects. The first Nationwide Study of Asthma and Allergies in Korean Children, which utilized the Korean version of ISAAC written and video questionnaire, was conducted in 1995 and the second Nationwide Study of Asthma and Allergies in Korean Children was conducted in 2000, directed by the Korean Academy of Pediatric Allergy and Respiratory Diseases. We report here the prevalence of asthma and other allergic diseases in Korean children and adolescents, and show the changes that occurred over this 5 year period. We also describe the risk factors for development of these diseases in Korean children. We developed Korean versions of the ISAAC written (WQ) and video (AVQ) questionnaires for allergic diseases. In 1995, the enrolled population consisted of 25,117 children selected from 34 elementary school and 14,946 children selected from 34 middle school across the nation, the response rate was 94.8%. In 2000, 27,831 children selected from 34 elementary school and 15,214 children selected from 34 middle school, and the response rate was 96.4%. From these studies, we can confirm that increase of the prevalences of asthma, allergic rhinitis, and atopic dermatitis in Korea, except the prevalence of food allergy. Especially, the video questionnaire showed increases in the lifetime and 12 month prevalence rates of wheeze at rest, exercise- induced wheeze, nocturnal wheeze, nocturnal cough, and severe wheeze over this period of time in middle school children. In addition, the increase of prevalences of symptoms, diagnosis and treatment of atopic dermatitis was noted significantly. Risk factor analysis showed that body mass index (BMI), passive smoking and living with a dog or cat were associated with higher risk of wheeze. Also the occurrence of fever during infancy and the frequent use of antibiotics were associated with the risk of wheeze. In conclusion, during the 5 year period from 1995 to 2000, the prevalences of asthma, allergic rhinitis, and atopic dermatitis has increased in Korean children. BMI, passive smoking, living with a dog or cat, the fever episodes in infancy, and the frequent use of antibiotics in infancy are important risk factors to development of asthma and atopic dermatitis. In the near future, the birth cohort study will be needed to investigate the causes of this increase and the natural course of allergic diseases, then we develop the methods to control asthma and allergic diseases. Asthma and allergic diseases are one of the most common disorders in children. Due to its increased prevalence, as well as the increased morbidity and mortality from these diseases, asthma and allergic diseases have come to be recognized as a major worldwide public health issue. In addition, socioeconomic burden of asthma and allergic diseases has increased in Korea also. The International Study of Asthma and Allergies in Childhood (ISAAC) investigated the worldwide prevalence of asthma and other allergic diseases using simple standardized methods because of the comparison of asthma and allergic diseases between the countries. In Korea, several epidemiologic studies have been conducted to determine the prevalence of asthma in children. Although these studies showed increased prevalence of asthma among Korean children (from 3.4% in 1964 to 10.1% in 1989), these findings were based on data from small numbers of subjects. The first Nationwide Study of Asthma and Allergies in Korean Children, which utilized the Korean version of ISAAC written and video questionnaire, was conducted in 1995 and the second Nationwide Study of Asthma and Allergies in Korean Children was conducted in 2000, directed by the Korean Academy of Pediatric Allergy and Respiratory Diseases. We report here the prevalence of asthma and other allergic diseases in Korean children and adolescents, and show the changes that occurred over this 5 year period. We also describe the risk factors for development of these diseases in Korean children. We developed Korean versions of the ISAAC written (WQ) and video (AVQ) questionnaires for allergic diseases. In 1995, the enrolled population consisted of 25,117 children selected from 34 elementary school and 14,946 children selected from 34 middle school across the nation, the response rate was 94.8%. In 2000, 27,831 children selected from 34 elementary school and 15,214 children selected from 34 middle school, and the response rate was 96.4%. From these studies, we can confirm that increase of the prevalences of asthma, allergic rhinitis, and atopic dermatitis in Korea, except the prevalence of food allergy. Especially, the video questionnaire showed increases in the lifetime and 12 month prevalence rates of wheeze at rest, exercise- induced wheeze, nocturnal wheeze, nocturnal cough, and severe wheeze over this period of time in middle school children. In addition, the increase of prevalences of symptoms, diagnosis and treatment of atopic dermatitis was noted significantly. Risk factor analysis showed that body mass index (BMI), passive smoking and living with a dog or cat were associated with higher risk of wheeze. Also the occurrence of fever during infancy and the frequent use of antibiotics were associated with the risk of wheeze. In conclusion, during the 5 year period from 1995 to 2000, the prevalences of asthma, allergic rhinitis, and atopic dermatitis has increased in Korean children. BMI, passive smoking, living with a dog or cat, the fever episodes in infancy, and the frequent use of antibiotics in infancy are important risk factors to development of asthma and atopic dermatitis. In the near future, the birth cohort study will be needed to investigate the causes of this increase and the natural course of allergic diseases, then we develop the methods to control asthma and allergic diseases.
-
홍수종,심정연,조유숙,박재경,우빈,문희범 대한천식알레르기학회 1997 천식 및 알레르기 Vol.17 No.3
To investigate the imbalance of the cytokine production profile of T cells from atopic asthmatics, we measured concentrations of IL - 4, IL - 5 and IFN-r by ELISA method in the culture supernatants of peripheral blood mononuclear cells(PBMCs) and Dermato- phagoides pteronyssinus(Der p) 1 stimulated PBMCs from Der p sensitized atopic asthmatics, Der p sensitized.healthy atopics, non atopic asthmatics and healthy non atopics. The suppressive effect of IFN-r on cytokine production of Der p 1 stimulated PBMCs was also examined. The PBMCs from atopics showed higher IL 4 and IL 5 production in response to PHA +TPA and higher IFN-r production in response to Der p lq compared with non atopics. The Der p 1 stimulated PBMCs from atopics showed a tendency of increased IL 5 production in response to Der p 1 and higher IL 4 and IL 5 production in response to PHA+TPA compared with non atopics. IL 5 production of Der p 1 stimulated PBMCs from atopics was suppressed by IFN - r It is suggested t,hat an imbalance in IL 4, IL 5 and IFN -r production is a feature of the atopic state. The TH characteristics of allergen stimulated PBMCs could be regulated by IFN -r .
-
-
-
-
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
