최근 5년 동안 국내에서 분리된 Shigella sonnei의 항균제 내성 유형과 내성유전자형 분석

허완,이상조,권기석,장종옥,이중복,Huh, Wan,Lee, Sang-Jo,Kwon, Gi-Seok,Jang, Jong-Ok,Lee, Jung-Bok 한국미생물학회 2007 미생물학회지 Vol.43 No.1

This study has been carried out for investigating the relatedness of representative 135 Shigella sonnei strains isolated from 2000 to 2004 by using biotyping and antimicrobial resistance. All strains showed typical biochemical characterisics of Shigella strain. Among 135 strains,79 (58.5%) strains were biotype "g",54 (40.0%) strains were biotype "a" and 2 (1.5%) strains were biotype "e". The results of susceptibility test against 16 antimicrobial agents were like this. Most of strains were susceptible to AN, CIP, C and GM. 129 (95.6%) strains were resistant to SXT, 126 (93.3%) strains were resistant to TE and 122 (90.4%) strains were resistant to SM. One hundred thirty two (97.8%) strains were resistant to more than two antimicrobial agents. R28 type (antimicrobial resistance patterns 28: resistant to AM, SAM, TE, TIC, SXT, K, SM and AmC) were 42 strains (31.1%). The other strains were showed 33 kinds of R patterns. The results of $bla_{TEM}$, sulII, tetA and strA gene detection were coincided with phenotype of antimicrobial resistance by disk diffusion method. But some strains which had sulII and strA genes were not showed the resistance against SXT and SM.

전야前夜(권두시)

허완 기독교대한성결교회 활천사 2024 기독교와 인문학 Vol.853 No.12

감관총채벌레(Ponticulothrips diospyrosi)의 분포와 발생소장

허완,허혜순,윤지은,손준기,장현규,엄기백,박정규,Huh Wan,Huh Hye-Soon,Yun Ji-Eun,Son Jun-Ki,Jang Hyeon-Kyu,Uhm Ki-Baik,Park Chung-Gyoo 한국응용곤충학회 2006 한국응용곤충학회지 Vol.45 No.1

전국의 감 재배 농가를 대상으로 감관총채벌레(Ponticulothrips diospyrosi Haga et Okajima)의 피해 면적과 분포 및 방제방법을 설문 조사하였고, 경남 김해와 진주의 세 곳 단감원에서 끈끈이 트랩과 감관총채벌레에 의한 피해엽을 조사하여 각 태별 발생시기를 파악하였으며, 트랩의 위치와 트랩면의 방향에 따른 유살수의 차이를 비교하였다. 감관총채벌레는 단감뿐 아니라 떫은 감도 가해하였으며, 피해면적은 2000년 이후 급격히 증가하여 2005년에는 전국적으로 446 ha에 달하였는데, 피해면적은 경남>경북>전남 순이었다. 대부분의 농민들은 감관총채벌레를 방제하기 위하여 살충제를 살포하거나 피해 잎을 제거해주었다. 월동세대와 제1세대 성충은 각각 5월 중순과 6월 상 중순부터 피해엽내에서 관찰되었으며, 7월 중순 이후에는 피해엽 내에 감관총채벌레가 발견되지 않았다. 황색끈끈이 트랩에 의한 월동세대 성충의 유살최성기는 5월 중순이었고, 제1세대 성충의 유살최성기는 김해에서는 6월 중 하순, 진주에서는 6월 하순이었다. 과수원 중앙보다는 외곽에 설치한 트랩에 제1세대 성충이 더 많이 유인되었으나 유살시기에는 차이가 없었다. 끈끈이 판의 양면 중에서 과수원의 중앙보다는 소나무 숲으로 향한 면에 더 많은 성충이 유살되는 경향이 있었으나 통계적 차이는 없었다. The damaged area by and the distribution of Japanese gall forming thrips, Ponticulothrips diospyrosi Haga et Okajima (Thysanoptera: Phlaeothripidae) in Korea were surveyed based on the answers of persimmon growers to questionnaire. Seasonal occurrence of each developmental stage was estimated by monitoring the individuals in damaged leaves, and effect of trap site and side of sticky board were studied by yellow sticky traps at three sweet persimmon orchards in Gimhae and Jinju (orchards A and B), Gyeongnam province, Korea in 2005. P. diospyrosi gave damage to astringent persimmon as well as sweet one. The damaged area of persimmon orchard has sharply increased since 2000, reaching 446 ha nationwide; Gyeongnam province was most severely damaged, followed by Gyeongbuk and Jeonnam provinces. Most of the farmers answered that they sprayed insecticides and removed the damaged leaves to control P. diospyrosi. The overwintered adults were observed in the damaged-rolled leaves from mid May and the 1st generation adults from early-mid June. No thrips were observed in the damaged leaves after mid July. Yellow sticky trap data showed that peak adults catch of the overwintered generation was mid May, and that of the 1st generation was mid-late June in Gimhae and late June in Jinju. Sticky traps set at orchard-pine forest border caught significantly more number of the 1st generation adults than those at the orchard center, even though timing of peak catches was not different between the two sites. There was a tendency of more catches on the side of sticky trap facing pine forest than the other side facing orchard center, even though the catches on both sides showed no statistical difference.

도시와 묘지 화장장운영의 문제점과 개선방안 - 서울특별시를 중심으로 -

허완 대한지방행정공제회 1983 도시문제 Vol.18 No.11

N/A

뷰티소재 개발을 위한 머루포도 에탄올 추출물의 생리활성 평가

허완,하헌용 대한미용학회 2024 대한미용학회지 Vol.20 No.1

. Grapes have been used as an important food resource and medicinal plant since the beginning of human civilization. Grapes are used as fresh fruits; dried grapes, which are easy to store, can be used to make alcoholic beverages through the process of fermentation. Scientific research on traditionally used natural raw materials is currently underway, and additional developments are anticipated. Muscat bailey A(MBA) is an improved variety from wild grapes and should be regarded as valuable as both a food ingredient and as a cosmetic ingredient. The purpose of this study was to examine the usefulness of grapes to the human body, the effects on antioxidant effects, cytotoxicity, and physiological factors involved in skin moisturization. Significant results were obtained in the evaluation of the antioxidant effect using the DPPH assay. The results from evaluation of HAS 2 gene expression in relation to the skin moisturizing effect confirmed an increase of approximately % compared to the control group. Hyaluronic acid (HA) is a polysaccharide involved in water retention in the human body, and hyaluronidase is an enzyme involved in decomposition of hyaluronic acid. Therefore, we examined the question of whether hyaluronidase can be inhibited by MBA; the results indicated approximately % greater effectiveness compared to the positive control. Based on these experimental results, recognition of the value of MBA as a raw material for maintaining skin health is anticipated.

연구논문 : 서울시 위생행정고찰 - 특수시책을 중심으로 약설 -

허완 대한지방행정공제회 1982 地方行政 Vol.31 No.345

논문 , 자료 / 연구논문 : 국산차 장려방안

허완 대한지방행정공제회 1983 地方行政 Vol.32 No.352

[시] 요절, 풋감 外 1편

허완 인천작가회의(작가들) 2023 작가들 Vol.- No.86

톱다리개미허리노린재의 집합페로몬에 의한 노린재류의 난기생봉 Ooencyrtus nezarae의 유인과 발생소장

허완,박정규,Huh Wan,Park Chung-Gyoo 한국응용곤충학회 2005 한국응용곤충학회지 Vol.44 No.2

경남진주의 콩포장과 대학 캠퍼스에서 톱다리개미허리노린재의 집합페로몬을 사용하여 난기 생봉인 Ooencyrtus nerarae(Hymenoptera: Encyrtidae)의 발생소장을 조사하였다. 2003년과 2004년에 대학 캠퍼스에 설치된 트랩에 유살된 암컷의 수는 수컷 유살수의 317.5배와 103.4배이었고, 2004년 콩포장에서는 12.6배이었다. 콩포장에서 O. nezarae의 암컷은 8월 하순부터 유살되기 시작하여 9월 20일 전후에 가장 많이 유살되었다. 2003년과 2004년에 대학 캠퍼스에서는 8월 중하순부터 밀도가 증가하여 9월 상중순에 최성기를 나타내었다. 두 조사지역에서 모두 10월 이후에는 거의 발생하지 않았다. 톱다리개미허리노린재의 집합페로몬 세 성분, 즉 (E)-2-hexenyl (Z)-3-hexenoate(E2HZ3H)와 (E)-2-hexenyl (E)-2-hexenoate(E2HE2H) 및 myristyl isobutyrate(MI) 중에서 E2HZ3H 만이 O. nerarae에 대해 유인력을 나타내었다. E2HZ3H:E2HE2H:MI를 7:36:7로 배합한 것보다는 20:20:10이나 16.7:16.7:16.7으로 배합한 트랩에 더 많은 수가 유인되었는데, 이는 배합된 E2HZ3H의 량 때문인 것으로 추정된다. Seasonal occurrence of the egg parasitoid of bugs, Ooencyrtus nezarae (Hymenoptera: Encyrtidae), was monitored at soybean fields and university campus using traps baited with aggregation pheromone of bean bug, Riptortus clavatus, in Jinju, Gyeongnam province, Korea. The female O. nezarae captured at university campus in 2003 and 2004 was 317.5 and 103.4 times as many as males, respectively, and it was 12.6 times at soybean field in 2004. The female began to occur from late August, showing its peak around September 20 at soybean field in 2004. At university campus in 2003 and 2004, the female began to occur from mid or late August, and showed its peak early or mid September. At the two monitoring sites the catches of female sharply declined after October The E2HZ3H only showed attractiveness to O. nezarae, among three components of the aggregation pheromone of bean bug, (E)-2-hexenyl (Z)-3-hexenoate (E2HZ3H), (E)-2-hexenyl (E)-2-hexenoate (E2HE2H), and myristyl isobutyrate (MI). A mixture in a ratio of 20:20:10 or 16.7:16.7:16.7 of E2HZ3H:E2HE2H:MI attracted significantly more females than the mixture of 7:36:7 ratio did. This higher attractiveness of the former two blends may be attributed to the higher amounts of E2HZ3H in the blends.

일장과 온도 처리가 톱다리개미허리노린재 수컷 성충의 휴면종료에 미치는 영향

허완,박정규,Huh, Wan,Park, Chung-Gyoo 한국응용곤충학회 2010 한국응용곤충학회지 Vol.49 No.3

톱다리개미허리노린재, Riptortus pedestris (=clavatus) Fabricius (Hemiptera: Alydidae)는 성충으로 월동휴면을 한다. 전형적인 장일형 곤충으로 일장 시간이 약 13.5시간 이하의 단일조건에서 휴면이 유기된다. 2006년 10월과 11월에 월동중인 수컷 성충을 채집하여 휴면종료에 미치는 온도와 일장 조합의 효과를 조사하였다. 즉, (1) HTLD = $25^{\circ}C$, 14L:10D에 1, 2, 3주 및 30일 처리, (2) HTSD = $25^{\circ}C$, 10L:14D에 1, 2, 3주 처리, (3) LTLD$\rightarrow$HTLD = $8^{\circ}C$, 14L:10D에 1, 2, 3주 처리 후 HTLD 처리, (4) LTSD$\rightarrow$HTLD = $8^{\circ}C$, 10L:14D에 1, 2, 3주 처리 후 HTLD 처리의 네 가지 조합으로 처리하였다. 수컷의 집합페로몬 분비 여부로 휴면종료 여부를 판단하였다. HTSD 조건에서는 페로몬을 분비하지 않았다. 그러나 HTLD 조건에 21일 이상 처리된 수컷은 무처리나 HTSD 처리 수컷에 비해 많은 량의 페로몬을 분비하였다. 고온장일 처리 7일부터 집합페로몬 성분인 E2HZ3H, E2HE2H 및 TI를 분비한 개체의 비율이 각각 14%, 29%, 100%에서 30일째에는 83%의 개체가 세 성분 모두를 분비하였다. 저온처리 없이 단순히 고온장일 조건에서 사육한 것들이 저온처리(LTLD$\rightarrow$HTLD 또는 LTSD$\rightarrow$HTLD)를 받은 것보다 페로몬을 분비하는 개체의 비율이 높았다. The bean bug, Riptortus pedestris (=clavatus) Fabricius (Hemiptera: Alydidae) is a serious pest of soybean in many countries. It enters a reproductive diapause in the winter by short day length (<13.5 h.) in Korea and Japan. The combined effects of temperatures ($25^{\circ}C$ and $8^{\circ}C$) and day lengths (10L:14D and 14L:10D) upon the termination of the male's diapause were studied. The combinations are (1) HTLD = $25^{\circ}C$, 14L:10D treatment for 1, 2, 3 weeks and 30 days; (2) HTSD = $25^{\circ}C$, 10L:14D treatment for 1, 2, and 3 weeks; (3) LTLD$\rightarrow$HTLD = $8^{\circ}C$, 14L:10D treatment for 1, 2, and 3 weeks followed by HTLD treatment; (4) LTSD$\rightarrow$HTLD = $8^{\circ}C$, 10L:14D treatment for 1, 2, and 3 weeks followed by HTLD. The amount of aggregation pheromone components secreted was adopted as the criteria for diapause termination in males. Males did not secrete aggregation pheromone under HTSD conditions. However, the males treated with HTLD for more than 21 days secreted significantly higher amounts of aggregation components, (E)-2-hexenyl (Z)-3-hexenoate and (E)-2-hexenyl (E)-2-hexenoate, compared to the control or HTSD treatments. The three components of the aggregation pheromone were secreted by the 14%, 29%, and 100% males treated with HTLD for 7 days, respectively. However more than 83% of the HTLD-treated males secreted all three components by the $30^{th}$ day under the treatment. In comparison, the pheromone amounts secreted by the males treated with LTLD$\rightarrow$HTLD or LTSD$\rightarrow$HTLD were equal or less than those secreted by males with continuous HTLD treatment.