한국의 양식대하에서의 흰반점증상 바이러스감염의 특징

허문수,Heo Moon-Soo 한국생명과학회 2005 생명과학회지 Vol.15 No.2

대하 새우양식장의 대하에 흰반점 증상을 나타내는 원인바이러스는 막대형의 이중막을 가지고 있었으며, 전자 현미경 관찰 결과 평균 크기가 $250\~300\times70\;nm$였고, 조직학적 병변은 위상피 등에서 핵이 비대해지는 것이 관찰되었다. 공격실험에서는 건강체의 새우에 많은 누적 폐사율을 보였다. 원인 바이러스 단백질은 21개의 밴드를 보였으며, 핵산 분석 결과 total 분자량은 114 kb로 나타났다. The virions of causative virus for white spot syndrome in cultured Fresh shrimp, Penaeus chinensis were rod-shaped, double envelope. An average size of the virion was 70 nm in diameter and $250\~300$ nm in length. Histopathological test of affected stomach, heart, and lymphoid organ revealed nuclear hypertrophy. Infectivity trials carried out by injection and feeding with purified virus revealed high cumulative mortality to healthy shrimp. The twenty one different protein species were detected in the analysis of virion. The length of total DNA from the purified virus particles were detected as a single band, double-stranded DNA molecule of approximately 114 kb.

학술발표회 : 8) 어병분야 , 구두발표 ; 양식대하 ( Penaeus chinensis ) 감염 White spot baculovirus ( WSBV ) 의 병원성 및 병리조직학적 특성

허문수,양병규,손상규 한국국제경제학회 2001 춘계 수산관련학회 공동학술대회발표 요지집 Vol.0 No.-

제주산 양식넙치 (Paralichthys olivaceus)로부터 분리된 $\beta$-용혈성 연쇄구균 ($\beta$-Streptococcsus spp.)의 특성

허문수,송춘복,이제희,여인규,전유진,이정재,정상철,이기완,노섬,최광식,이영돈,HEO Moon-Soo,SONG Choon Bok,LEE Jehee,YEO In-Kyu,JEON You-Jin,LEE Jung-Jae,CHUNG Sang-Chul,LEE Ki-Wan,RHO Sum,CHOI Kwang-Sik,LEE Young Don 한국수산과학회 2001 한국수산과학회지 Vol.34 No.4

2000년도 2회에 걸쳐 제주도 일원 넙치 양식장을 대상으로 감염어에서 분리된 연쇄구균에 대한 특성시험 결과를 요약하면 다음과 같다. 감염어로부터 분리된 연쇄구균은 형태적으로 연쇄상의 gram 양성의 구균이었고, catalase와 oxidase에 음성이었으며, 용혈성 시험에서는 $\beta$-용혈성을 나타내었다. 감염어에서 각 장기별 생균수에서는 환부, 신장, 혈액 그리고 뇌순으로 연쇄구균이 검출되었다. 약제 감수성에서는 ampicillin, ciprofloxicin 둥에 감수성이 있었지만 erythromycin 등에는 감수성이 없었다. 복강에 주사하여 병원성 시험을 한 결과 10일 이내에 전량 폐사하는 강한 병원성을 보였다. 감염균의 용혈성에 대한 특성은 $50^{\circ}C$ 30분의 배양에서 용혈활성이 마르게 증가되어 최대값에 도달되었고 또한 $pH 5.5\~6.5$에서 높은 활성값을 보였다. Streptococcus spp. of bacterial pathogen of fish were isolated from the cultured flounder (Paralichthys olivaceus) in fish farm of Jeju Island. Clinical signs of the infected flounder which are the most commons symptoms are as follows: erratic swimming, darkening of the body colour, unilateral or bilateral exophthalmia, corneal opacity, hemorrhages in the opercular and the bases of the fins, and the ulceration of the body surface. Biochemical characteristics of pathogenic fish Streptococcus spp, were gram positive, spherical form, catalase negative, oxidase negative and $\beta$-haemolytic, respectively, The viable cells counted from the tissue of the diseased flounder were the largest in the order of the ulcer, the kidney, the blood and the brain, The drugs used were ampicillin, ciprofloxacin, doxycycline, gentamycin, tetracycline, erythromycin, streptomycin and oxytetracycline, Streptococcus spp. were found to be sensitive to ampicillin, ciprofloxacin, doxycycline and gentamycin, but were resistant to tetracycline, erythromycin, streptomycin and oxytetracycline. The pathogenicity of Streptococcus spp. on the cultured flounder with an abdominal cavity injection was high. The haemolytic activity of the toxin against the sheep red blood cells reached the maximum after 30 min incubation at $37^{\circ}C$ or $50^{\circ}C$. The toxin showed highest activity at pH $5.5\sim6.5$.

부동산프랜차이즈제도의 개선방안에 관한 연구

허문수,이창석 강남대학교 사회과학연구소 2007 사회과학논총 Vol.23 No.-

Vibrio furnissii로부터 세포외 용혈소의 정제 및 특성

허문수,HEO Moon-Soo 한국수산과학회 2001 한국수산과학회지 Vol.34 No.5

The extracellualr hemolysin produced by pathogenic Vibrio furnissii was isolated and purified by ammonium sulfate precipitation and followed by DEAE-Sephadex A-50 (1st and 2nd) ion-exchange and Sephadex G-100 gel permeation chromatography. The molecular weight of the purified hemolysin was 61 kDa. The enzyme activity was inactivated by heating at 60 for 5 min, and inhibited by additions of $Cu^{2+},\;Zn^{2+}$ and a high concentration of cholesterol. Lysis of human erythrocytes by the enzyme was temperature dependent, and optimal temperature of the enzyme was $37^{\circ}C$. The purified hemolysin was unstable at pH 6.0, 6.5 and 10.0. The optimal concentration of human erythrocytes on hemolysins was about $1.5\%$. The purified hemolysin was active for erythrocytes from three animal species including mouse, rabbit and rat, and was the most active against rabbit erythrocytes. B blood group of human erythrocytes also showed a high sensitivity to the enzyme. 병원성 비브리오 속인 Vibrio furnissii의 용혈소를 분리정제하고 그 물리화학적 특성, 생물학적인 그리고 면역학적인 상호관련성을 검토하여, 다음과 같은 결과를 얻었다. 용혈소의 정제는 ammonium sulfate precipitation, DEAE-Sephadex A-50 (1st와 2nd)와 Sephaedx G-100 gel permeation chromatography를 거쳐 738배로 정제되었으며 회수율은 각각 $11.6\%$ 와 $5.2\%$로 나타났다 전기영동법으로 용혈소의 정제를 확인한 결과 단일 밴드의 형성을 관찰할 수 있었다. 분자량은 61 kDa이였으며 또한 SDS-PAGE 결과 subuint는 존재하지 않았다. 정제된 용혈소는 열에 불안정한 이열성 용혈소이며 $Cu^{2+},\;Zn^{2+}$와 cholesterol에 의해 용혈활성이 모두 저해되었다. 용혈기작에 있어서는 온도의존성 용혈기작으로서 최적 용혈의 온도는 $37^{\circ}C\sim47^{\circ}C$로 나타났다. 정제용혈소의 pH 안정성은 pH 6.0, 6.5, 10.0에서 각각 불안정하였다. 용혈활성에 있어서 최적 혈구 농도는 $1.0\sim2.0\%$였다. 또한 각종 동물 혈구의 감수성 시험 결과 두 용혈소 모두 토끼에서 가장 감수성이 높았으며 사람의 혈구에서는 B형 혈액형에서 다소 높은 감수성을 나타내었다.

부동산경매제도의 절차상 문제점과 개선방안

허문수,이동영 한국부동산경영학회 2012 부동산경영 Vol.6 No.-

Real estate auction, a way for purchasing real estate, has a strong point- people can purchase real estate it for cheap price, but it has a weak point-people suffers from damage due to difficult right analysis. Recently, end-users through real estate auction have a high concernment. It is why they think auction to be a way of financial technology. But high concernment does not always bring about high success rate. As usual, in markets, there are more people suffering from failure than those who succeed in investment through real estate auction. People’s concernment on real estate auction increases gradually, but real estate auction is not activated, yet. As a result of material analyses, this thesis leaded to the result as followings. First, administrative officials have to be given the right of taking in and out, the right of investigation on occupants and debtors, and extensive compelling power to enforce to submit documents and to confirm subjectively inner structure and use. It will enable them to make written present condition. Second, if an auction procedure, aimed at carrying out legal rights of creditors, should be delayed, trust on national enforcement system is lowered. So, a disturbance of disposal by a complaint has to be got rid of with improved means. Third, the way to solve unpaid disposal is not standardized between courts. So, the cases have to be solved, basing on supreme regulations, for example the materials have to be disposed by special sale auction, that is, by delay interest rate added. In the end, as for the status of debtors, a lot of problems on laws relating to the current lease and the procedure of auction are exposed. The debtors’ seniority has to be secured. So, a item has to be established, saying that even if a debtor should win lessor in deposit return demand lawsuit and applies for auction for a real estate, counter power for the thirty party has not to be conceited, if only the lessor should dwell in the house till the time of auction application and deliver the house at the time of auction. As a result, the real status of the current real estate auction system operation and problems in carrying out it have to be continuously grasped continuously so that the system can be fixed as a system and improvement ways have to be established. Then people can trust the real estate auction system. In the future, the system improvement way has to be explored continuously.

보리새우류의 바이러스 감염증의 진단

허문수,정초록,장경립 한국생명과학회 1999 생명과학회지 Vol.9 No.4

Baculovirus(WSBV) was isolated from infected Penaeide was collected from shrimp farm at southern sea of Korea from 1993 to 1995. The Infectious virus was purified and used for diagnosis of infected shrimp. Anti-viral serum were used for immunological detection as enzyme linked immunoabsorbent assay(ELISA) and indirect fluorescent antibody technique(IFAT). In IFAT, stomach, lymphoid organ and antenae gland of infected shrimp showed fluorescent reaction. In ELISA, tissues of spontaneously infected shrimp appeared higher O.D. values than in artificial infected shrimp. Primer set was constructed from sequence of 420bp of cloned Baculovirus(WSBV) genome. Specific band for infected shrimp was detected in Polymerase chain reaction(PCR)

Vibrio vulnificus ATCC 27562의 16S rRNA 유전자의 PCR과 제한효소절단 방식

허문수,정초록 한국생명과학회 1998 생명과학회지 Vol.8 No.2

A pair of designed primers (sequences from Gene Bank) amplified 16S fRNA gene of V. vulnificus within polymerase chain reaction (PCR) machine. This PCR product is about 1.3kb DNA fragment. Six enzymes (BamH I, Alu I, Sau3A I, Hind III, Sal I, Sma I) were used for restriction pattern analysis of amplified 16S rRNA gene of V. vulnificus ATCC 27562. Digested fragments are resolved by 3% agarose gel. BamH I did not show digested fragment so, there was no cutting site of BamH I in PCR product. Alu I produced three small fragments from 400 bp to 200 bp. Sau3A I produced three fragments larger than Alu I from 70 bp and 500 bp. One of fragments of Sal I was same with 500 bp of Hind III fragment and the other was 750 bp. Sma I showed two fragments of 800 bp and 470 bp. The profile of digested fragments of 16S rRNA of V.vulnificus ATCC 27562 will may be able to use standard profile for identification of V. vulnificus. Vibrio unlnificus ATCC 27562의 16S rRNA 유전자를 PCR법과 제한효소절단법으로 분석 하여 얻은 결과는 다음과 같다. 1. 고안된 한쌍의 primer로 PCR을 시행하여 얻은 산물은 약 1.3Kb 였다. 2. PCR산물을 여섯가지의 제한 효소로 절단하여 얻은 단편들은 아래와 같다. BamH I: 어떤 restriction fragment도 만들지 않았다. Alu I : 약 400bp와 200bp의 두가지 fragment를 생산하였다. Sau3A I : 약 70bp에서 450bp 사이에 세가지 fragment를 생산하였다. Hind III : 약 800bp와 500bp의 약간 큰 두가지 fragment를 생산하였다. Sal I : 약 500bp와 750bp의 두가지 fragment를 생산하였다. Sma I : 약 800bp와 470bp의 두가지 fragment를 생산하였다.

부동산 셀링포인트에 관한 일고

허문수,이창석 강남대학교 사회과학연구소 2009 사회과학논총 Vol.25 No.-

부동산 경매제도의 문제점과 그 과제

허문수 강남대학교 사회과학연구소 2008 사회과학논총 Vol.24 No.-