GST의 Sprague-Dawley Rat를 이용한 단회 경구투여 독성시험 및 4주 반복 경구투여 용량결정시험

한종민,홍지희,이혜영,정인철,진미림,김승형,박양춘,Han, Jong-Min,Hong, Jee-Hee,Lee, Hye-Yeong,Jung, In-Chul,Jin, Mi-Rim,Kim, Seong-Hyeong,Park, Yang-Chun 대한한방내과학회 2013 大韓韓方內科學會誌 Vol.34 No.4

Objectives : This study aimed to evaluate the single oral dose toxicity and four weeks repeated dose determination of Gamisasangja-tang (GST) in male and female Sprague-Dawley rats. Methods : In the single oral toxicity study, rats were orally administered a single dose of 0 and 5,000 mg/kg GST. There were 5 rats in each group. After single administration, mortality, clinical signs, body weight changes and gross pathological finding were observed for 14 days. In the 4-weeks repeated oral dose determination study, rats were orally administered a single dose of 0, 1,250, 2,500 or 5,000 mg/kg GST. There were 5 rats in each group. Mortality, clinical signs, body weight changes, food consumption and gross pathological finding were observed for 28 days. Organ weight, clinical chemistry and hematology were tested after 28 days. Results : There was no mortality in either of the two studies. There were also no significant differences in clinical sign, body weight, organ weights, hematological or serum chemical parameters between the GST and control groups. Conclusions : The results obtained in this study suggest that the 50% lethal dose of GST is over 5,000 mg/kg, so this finding would be expected to provide scientific evidence for the safety of GST.

한국 연근해산 두족류 (Todarodes pacificus and Octopus minor) 시엽내 Dopamine 및 $Calbindin-D_{28K}$의 분포에 관한 면역전자현미경적 연구

한종민,장남섭,Han, Jong-Min,Chang, Nam-Sub 한국현미경학회 2002 Applied microscopy Vol.32 No.2

한국 연근해산 살오징어와 서해낙지의 시엽에서 신경전달물질을 분비하는 neuron의 특성 및 기능을 확인하기 위해 rabbit anti-dopamine (TH)과 rabbit anti-calbindin-$D_{28K}$ 등의 항체를 이용한 면역염색과 면역금지법을 시행한 결과는 다음과 같았다. 항-dopamine을 사용한 면역염색 결과 살오징어에서는 외과립세포층 상단에 위치한 대형 무축삭세포와 수질부의 섬을 이루는 세포들 중 $2{\sim}3$개의 비교적 소량의 세포만이 양성반응을 보인 반면, 서해낙지에서는 외과립세포층 상단 및 중간 부위의 $2{\sim}3$개의 세포와 수질부섬을 이루는 5개 이상의 세포에서 강한 양성반응을 보였다. 항-calbindin-D28k인 경우는 살오징어의 외과립세포층 상단에 위치한 $2{\sim}3$개의 소형 무축삭세포와 내과립세포층 하단에 위치한 $1{\sim}2$개의 세포에서 양성반응을 보인 반면, 서해낙지인 경우는 외과립세포층에서 4개 정도의 세포에서 양성 반응을 보였으나, 내과립세포층에서는 반응을 확인할 수 없었다. 면역금지법을 시행한 결과 살오징어에서는 항-dopamine과 항-calbindin에 면역반응을 보인 세포들의 세포질 $0.5{\mu}m^2$당 $17{\sim}26$개 정도의 비교적 많은 수의 금입자가 표지된 반면, 서해낙지에서는 세포질 $0.5{\mu}m^2$당 평균 10개 정도인 소량의 금입자만이 표지되어, 살오징어가 서해낙지에 비해 표지된 금입자가 거의 2배 정도 많았다. In this study, we carried out immunostaining and immunogold labeling with rabbit anti-dopamine (TH) and rabbit anti-calbindin-$D_{28K}$ to examine the characteristics and functions of the neurons that secrete neurotransmitters in optic lobes of Todarodes pacificus and Octopus minor inhabiting the Korean waters. The obtained results are as follow. In the immunostaining with anti-dopamine, only a few of the large amacrine cells in an the upper part of an outer granule cell layer and the cells forming the islands of medulla showed positive reaction in Todarodes pacificus, while $2{\sim}3$ cells in the upper and middle parts of an outer granule cell layer and more than 5 cells in the islands of medulla reacted positively in Octopus minor. For the case of anti-calbindin case, $2{\sim}3$ small amacrine cells in the upper portion of the outer granule cell layer and $1{\sim}2$ cells which are located in the lower part of an inner granule cell layer showed positive reaction in Todarodes pacificus, while, in Octopus minor, 4 cells in the outer granule cell layer reacted positively, no immunoreactive cell being found in the inner granule cell layer. As a result of performing the immunogold labeling, relative large number ($17{\sim}26$) of gold particles were labeled per $0.5{\mu}m^2$ of the cytoplasm of the cells which showed the immunoreactivity to the anti-dopamine and anti-calbindin in Todarodes pacificus, however, small number (10) of gold particles were labeled in Octopus minor, which reach only half of the number in the Todarodes pacificus.

산업장 근로자의 직종별 생활습관과 스트레스의 평가

한종민,권소희,정해경,강홍구,송용선,이기남,Han Jong-Min,Kwon So-Hee,Jung Hae-Kyoung,Kang Hong-Gu,Song Yung-sun,Lee Ki-Nam 대한예방한의학회 2004 대한예방한의학회지 Vol.8 No.1

This study was conducted to evaluated the degree of job-related life style and stress of industrial workers to present fundamental materials of preventive oriental medicine. The medical examination with oriental medicine method was performed from October 14 to November 1, 2002 for the 474 industrial workers and general characters, life style and Psychosocial Well-being Index(PWI) were collected by using self-recording type questionnaire. The results were as follows; 1) All 474 of subjects were male, job type was distributed into technician, 37.8% of them, white collar worker, blue collar worker, research worker in an orderly fashion. 2) As a result of comparison between the job types in general characters, research worker group was the highest in education level, above 10 years group in employee duration and 1.5-2.99 million won group in monthly income was the largest group in all job type and the distribution was significantly different. 3) Analyzing the difference in life style according to the job type shows that significantly highest prevalence of drinking alcohol was found in blue collar workers, they drank alcohol 2-4 times a week and that workers did not smoke in all group of job type and the prevalence of exercise was significantly high in less than 1 time a week. 4) Analyzing the difference in the degree of stress according to the job type shows that blue collar worker group had the highest score in Factor 1, Factor 4 fields, technician group had the highest score in Factor 2 field and the differences were significant. Considering above results, the present study shows that there are difference in life style and stress according to the job type in industrial workers and that health management according to job type must be required to improve health condition and to prevent disease of industrial workers in the future.

만성폐쇄성폐질환 동물모델에 대한 射干湯 및 구성약물의 효과

한종민,양원경,김승형,박양춘,Han, Jong-Min,Yang, Won-Kyung,Kim, Seung-Hyeong,Park, Yang-Chun 대한한의학방제학회 2015 大韓韓醫學方劑學會誌 Vol.23 No.2

Objective This study aimed to evaluate the effects of Sagan-tang (SGT) on COPD mouse model. Methods The study was carried out by two ways (in vitro, in vivo). In vitro RAW264.7 cells (mouse macrophage) were used and analysed by flow cytometry, ELISA, Western blot. In vivo LPS and CSS challenged mice were used and its BALF had been analysed by cytospin image, FACS, ELISA, lung tissue by real-time PCR. Results In vitro, SGT maintained 80-100% rate of viablilty on 10 ~ 500 ㎍/㎖ concentration. In ELISA analysis with RAW264.7 cells, SGT significantly decreased NO over 30 ㎍/㎖. In flow cytometry, SGT 100 ㎍/㎖ dosage group displayed a tendency for decrease ROS. In Western blot analysis, SGT 100 ㎍/㎖ dosage group decreased NF-κB. In ELISA analysis, SGT significantly decreased TNF-α, IL-6 over 200 ㎍/㎖. In vivo SGT 200 ㎎/㎏ dosage group, application of SGT significantly decreased increase of neutrophils, TNF-α, IL-6 in BALF, muc5AC, TGF-β, TNF-α, expression of mRNA in lung tissue and histological lung injury. Conclusion This Study suggests usability of SGT for COPD patients by controlling lung tissue injury.

한국 연근해산 두족류 (Todarodes pacificus And Octopus minor) 망막 (Retina)의 미세구조 I

한종민,장남섭,Han, Jong-Min,Chang, Nam-Sub 한국현미경학회 2002 Applied microscopy Vol.32 No.1

살오징어 (Todarodes pacif icus)와 서해낙지 (Octopus minor)의 망막은 외절부, 간상기저부, 내절부 그리고 망상층 등 4개의 층으로 구분되었고, 망막의 두께는 오징어 ($385{\sim}400{\mu}m$)에서보다 낙지 ($400{\sim}420{\mu}m$)에서 $20{\mu}m$ 정도 더 두터웠다. 망막은 시각세포와 지지세포로 구성되어 있는데, 서해낙지의 지지세포 상단에는 미세융모 (길이 $0.6{\sim}0.7{\mu}m$)가 밀생된데 비해 살오징어에서는 확인되지 않았다. 망막을 구성하는 시각세포와 지지세포들은 광선을 차단하는 색소과립들을 소지하고 있었는데, 살오징어인 경우 시각세포의 색소과립(크기, $2.0{\times}0.5{\mu}m$ 정도)이 지지세포의 색소과립 (크기, $1.0{\times}0.3{\mu}m$ 정도)에 비해 컸으나, 서해낙지에서는 두 세포간 색소과립의 크기 ($0.8{\times}0.6{\mu}m$ 정도)가 비슷하였다. 시각세포의 세포질 상단부에서 빗살모양의 미세융모들이 정육각형 구조 (직경, 60 nm)인 rhabdome을 형성하였는데 한 개의 rhabdome은 4개의 rhabdomere로 구성되어 있으며, 전체 rhabdome의 총 단면적은 서해낙지가 살오징어에 비해 두 배정도 컸다. 망상층을 구성하는 연접체는 살오징어인 경우 전자밀도가 높은 핵을 포함하는 소포 (electron dense-core vesicle)와 전자밀도가 낮은 소포 (electron lucent vesicle)들로만 형성된 연접체 등 두 종류를 소지한 반면, 서해낙지에서는 전자밀도가 높은 소포(electron dense vesicle)와 전자밀도가 낮은 소포들이 혼합된 형과 전자밀도가 낮은 소포들로만 이루어진 연접체 등 두 종류를 소지하고 있어 두 종간 차이가 확인되었다. The retinae of Todarodes pacificus and Octopus minor are divided into four layers that are an outer segment, a rod base region, an inner segment, and a plexiform layer, respectively. The retina of Octopus minor is about $20{\mu}m$ thicker ($400{\sim}420{\mu}m$) than that of Todarodes pacificus ($385{\sim}400{\mu}m$). A retina is composed of visual cells and supporting cells. The microvilli of length $0.6{\sim}0.7{\mu}m$ are packed densely on top of the supporting cells of Octopus minor while they are not found in Todarodes pacificus. The visual cells and supporting cells have pigment granules that exclude light. In case of Todarodes pacificus, the pigment granules of the visual cell are larger ($2.0{\times}0.5{\mu}m$) than those of the supporting cell ($1.0{\times}0.3{\mu}m$). But, the sizes of both cells are similar in Octopus minor. In the upper portion of a visual cell, microvilli shaped like a comb are forming a rhabdome (diameter, 60 nm) of a hexagonal structure. The rhabdome consists of 4 rhabdomere and the total area of a rhabdom of Octopus minor is larger than that of Todarodes pacificus. The synaptosome constructing a plexiform layer in Todarodes pacificus are divided into two types, each of which possess electron dense-core vesicles and electron lucent vesicles, respectively. Octopus minor also has two types of synaptosomes but each type comprises a mixture of electron dense vesicles and electron lucent vesicles, and electron lucent vesicles only, respectively, which is different from the case of Todarodes pacificus.

폐타이어 고무 분말을 이용한 재활용 고무 아스팔트 적용 연구 사례

한종민,김두병,Han, Jong-Min,Kim, Doo-Byong 한국건설순환자원학회 2015 한국건설순환자원학회지 Vol.10 No.4

한국 연근해산 두족류 (Todarodes pacificus and Octopus minor) 시엽 (Optic lobe)의 미세구조

한종민,장남섭,Han, Jong-Min,Chang, Nam-Sub 한국현미경학회 2002 Applied microscopy Vol.32 No.2

Optic lobes of Todarodes pacificus and Octopus minor are largely divided into cortex and medulla, the cortex being composed of three layers (an outer granule cell layer, a plexiform layer, and an inner granule cell layer). The cortex of Todarodes pacificus is about $420{\sim}450{\mu}m$ thick, being $170{\sim}200{\mu}m$ thicker than that of Octopus minor of which thickness is about $250{\sim}290{\mu}m$. In the outer granule cell layer of Todarodes pacificus, three types of nerve cells (type-A, type-B and type-C) and neuroglial cells that surround or contact with the neurons are observed, while in the outer granule cell layer of Octopus minor, two types of nerve cells (type-A and type-B) and a single type of neuroglial cells are observed. In a plexiform layer, a presynaptic bag and nerve endings are connected to each other, consequently forming various types of synaptosomes. The synaptosomes of Todarodes pacificus contain electron dense vesicles, electron dense-core vesicles and electron lucent vesicles, either individually or in a mixture. On the other hand, three types of synaptosomes a mixture of electron dense-core vesicles and electron lucent vesicles, electron lucent vesicles only, and electron dense-core vesicles only are observed in Octopus minor. The structures of the inner granule cell layer are almost similar in the two species. It is composed of two types of nerve cells (type-A, type-B) and a single type of neuroglial cells. In the medulla of Todarodes pacificus, the cells of $7{\times}5{\mu}m$ are arranged to a line and form the palisade cell layer, but these are not observed in Octopus minor. 살오징어와 서해낙지의 시엽은 피질부와 수질부로 크게 나눌 수 있었고, 피질부는 3층(외과립세포층, 망상층, 그리고 내과립세포층)으로 구성되어 있었다. 피질부의 두께는 오징어에서 약 $420{\sim}450{\mu}m$ (외과립층, $100{\mu}m$; 망상층, $170{\sim}200{\mu}m$ 그리고 내과립층, $150{\mu}m$) 정도였고 낙지인 경우는 약 $250{\sim}290{\mu}m$ (외과립세포층, $50{\sim}70{\mu}m$; 망상층, $100{\sim}120{\mu}m$; 내과립세포층, $100{\mu}m$) 정도로 관찰되어 오징어가 낙지에 비해 $170{\mu}m$ 정도 더 두터웠다. 살오징어의 외과립세포층에서는 3종류의 신경세포(A형, B형 그리고 C형)와 이들을 감싸거나 인접되어 있는 신경교세포들이 관찰되었고, 서해낙지에서는 2종류의 신경세포(A형과 B형)와 1종류의 신경교세포가 관찰되었다. 망상층에는 전연접자루와 신경말단들이 서로 연접되어 다양한 형태의 연접체를 형성하였는데, 살오징어에서는 전자밀도가 높은 소포, 과립소포, 그리고 투명소포 등이 혼재되어 있거나 한 종류만을 포함하는 경우 등 다양한 반면, 서해낙지에서는 투명소포만을 소지한 경우와 과립소포만을 소지한 경우, 그리고 투명소포와 과립소포들이 혼합된 경우 등 3종류의 연접체가 주로 관찰되었다. 내과립세포층은 구조적으로 두 종에서 거의 비슷한 형태이며 2종류의 신경세포(A형과 B형)와 1종류의 신경교세포로 구성되어 있었다. 살오징어의 수질부에서는 크기가 $7{\times}5{\mu}m$ 정도인 세포들이 일렬로 배열되어 있어 울타리세포층을 형성하였으나 서해낙지에서는 이들이 관찰되지 않았다.

삼중 튜브형 잠열 에너지 저장 시스템의 실린더 종횡비에 따른 용융 속도 및 열 전달 특성 분석

한종민(Jong Min Han),박성현(Seong Hyun Park),하만영(Man Yeong Ha) 대한기계학회 2021 大韓機械學會論文集B Vol.45 No.11

본 연구에서는 태양열 에너지 분야에서 주로 사용되는 잠열 저장 시스템의 삼중 튜브 열 교환기에서 실린더 내측 벽의 종횡비에 따른 용융 속도와 열전달 특성을 수치 해석적으로 분석하였다. 종횡비에 따른 용융 속도를 비교하기 위해 시간 경과에 따라 상 변화 물질의 액상 분율 변화와 온도 및 속도변화를 비교 분석하였다. 종횡비가 가장 클 때(aspect ratio=2.5) 초반 용융 속도는 빨랐으나, 그 이후의 과정에서는 종횡비가 가장 작을 때(aspect ratio=0.4) 용융 속도가 빨랐다. 내부 상 변화 물질의 평균 온도는 종횡비에 관계없이 증가하였으며, 총 용융시간은 종횡비에 관계없이 일정하였다. In this study, the melting rate and heat transfer characteristics of the inner cylinder in the triple-tube heat exchanger of a latent heat thermal energy storage (LHTES) system that primarily stores solar energy were numerically analyzed based on the cylinder aspect ratio. The melting rates were compared with respect to the aspect ratio by analyzing the changes in the liquid fraction, temperature, and velocity of phase change materials (PCMs) over time. When the aspect ratio was 2.5, the initial melting rate was the highest; however, it was observed that the subsequent melting rate was the highest when the aspect ratio was 0.4. The average temperature of the internal PCMs increased regardless of the aspect ratio. Further, the total melting time was constant regardless of the aspect ratio.

반도체 생산용 초순수 제조공정의 농축수 재이용에 따른 온실가스 발생량 평가에 관한 연구

한종민(Jong-Min Han),정진도(Jin-Do Chung),김산(San Kim) 한국산학기술학회 2018 한국산학기술학회논문지 Vol.19 No.9

21세기에 들어서면서 기후변화로 인한 환경변화가 심각해짐에 따라 온실가스 배출에 대한 관심도 높아지고 있다. 우리나라의 기온은 1904년 이후 2000년까지 약 1.5℃ 상승하였으며, 기후가 아열대 특성을 나타내는 것으로 조사되었다. 이에 따라 홍수 및 가뭄 등의 발생횟수가 증가하여 인간이 활용할 수 있는 용수가 매년 감소하고 있으며, 상수도 사용에 대한 비용은 매년 상승하고 있어 평소 버려지는 폐수의 재이용은 불가피한 상황에 직면해 있다. 본 연구는 반도체 공정에 투입되는 초순수 제조과정에서 발생되는 농축폐수를 재이용시스템을 설치하여 6개월간 운전한 월별 자료를 활용하여 온실가스 발생량 및 절감방안 등에 대하여 조사하였다. 조사결과 상수도 공급량은 월 평균 약 2,767㎥의 절감 효과가 있음을 알 수 있었다. 온실가스 발생량은 물재이용시스템의 전기사용량으로 인해 월 평균 약 1,329.07 ㎏-CO₂ 추가발생 하였지만, 상수도 사용량 절감에 따른 월 평균 918.64 ㎏-CO₂ 감축량을 반영하면, 월 평균 410.43 ㎏ -CO₂의 온실가스가 증가됨을 알 수 있었다. 물재이용시스템의 일부 공정을 개선한다면 온실가스 발생량은 증가가 아닌 월 평균 254.41 ㎏ -CO₂로 절감됨을 확인할 수 있었다. In the 21st century, human beings are becoming increasingly concerned about greenhouse gas emissions as the environment changes due to climate change become serious. The temperature of Korea has risen by approximately 1.5 ℃ from 1904 to 2000, and the climate is changing gradually to a subtropical climate. As a result, the frequency of floods and droughts increases, so that the water available to humans is decreasing every year, and the cost of using city water is rising every year. The reuse of wastewater that is normally abandoned is inevitable. This study examined the monthly data for 6 months of operation by installing a reuse system of concentrated wastewater (Re R/O System) that is generated during the process of manufacturing de-ionized water (DI-Water System) used in semiconductor processing. As a result of the survey, the city water supply saved approximately 2,767 ㎥ per month. The average annual greenhouse gas emissions was 1,329.07 ㎏-CO₂ per month due to the electricity consumption of the water reuse system. On the other hand, because of the reduction in city water supply, the average monthly average of 918.64 ㎏-CO₂ was reduced, and the greenhouse gas emissions were increased to 410.43 ㎏-CO₂ per month. If it improves some processes in the water reuse system, the amount of greenhouse gas emissions can be reduced by an average of 254.41 kg-CO₂ per month.

핀 길이 및 배열에 따른 잠열 저장 시스템의 용융 성능 및 열 전달 특성에 관한 수치적 연구

한종민(Jong Min Han),박성현(Seong Hyun Park),박용갑(Yong Gap Park),하만영(Man Yeong Ha) 대한기계학회 2021 대한기계학회 춘추학술대회 Vol.2021 No.5