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식품 : 미생물학 ; 한국 자생식물 추출물의 항산화 및 Aldo-keto Reductase Family 1 B10 효소 억제 효과
판철호 ( Cheol Ho Pan ),이주영 ( Joo Young Lee ),송대근 ( Dae Geun Song ),김종환 ( Jong Hwan Kim ),안수용 ( Soo Young Ahn ),배덕성 ( Deok Sung Bae ),김영훈 ( Young Hoon Kim ),이재권 ( Jae Kwon Lee ) 한국응용생명화학회 2009 Journal of Applied Biological Chemistry (J. Appl. Vol.52 No.4
판철호(Cheol Ho Pan),이은아(Eun A Lee),조도현(Do Hyun Jo),김수일(Su Il Kim) 한국응용생명화학회 1998 Applied Biological Chemistry (Appl Biol Chem) Vol.41 No.7
Chitinase, which is one of the pathogenesis-related proteins, was examined from Rehmannia glutinosa. Rehmannia chitinases were classified into basic and acidic groups by chitin affinity column chromatography. According to the chitinase pattern by native PAGE, basic chitinases of low Rf values were extracted more under an acidic condition (pH 2.9) than a basic condition (pH 8.8), while acidic chitinases having high Rf values were mainly detected in basic extracts. There were in total seven chitinase isoforms of three basic and four acidic isoforms in Rehmannia. The range of molecular weight of Rehmannia chitinase was from 28 to 32 kD. It showed the possibility of tissue specific expression of acidic chitinases and basic chitinases were mainly detected in root.
유색감자 홍영(Solanum tuberosum L.)의 입자 크기에 따른 반죽과 면의 물리적 특성
정화빈 ( Hwabin Jung ),판철호 ( Cheol Ho Pan ),윤원병 ( Won Byong Yoon ) 한국산업식품공학회 2015 산업 식품공학 Vol.19 No.3
The physical properties of raw and cooked dough for noodle with different sizes of red fleshed potato flour (RFPF) prepared by ball-milling and grinding were investigated. Microstructure images were taken to examine the structure of wheat flour (W) and RFPFs in dough. Thermal and texture properties were measured to investigate the effect of particle size on the physical properties of dough. Thermal properties of flours demonstrated that the highest degree of the damage in starch structure was found in ball-milled (BM) flour. Viscoelastic properties were evaluated using stress relaxation test. The addition of RFPF in dough showed a higher value of elastic components and shorter relaxation time. The increment of the lone spring modulus (Ee) for BM dough was 1346.66% whereas for the largest size of RFPF, the dough was 2543.75%. That different degree in the increment of viscoelastic properties indicates that highly damaged starch in BM showed less gelatinization compared to other RFPFs. The addition of RFPF increased the color parameter a (red-green) of dough and noodle from -3.46±0.17 to 6.08±0.13, respectively. This study suggested that very fine flour rather negatively affected the dough properties, whereas finding a suitable particle size plays an important role in controlling the quality of both dough and noodle.
초고압 처리에 의한 Gluten-free 메밀 반죽과 면의 신장성 및 조직감 특성 변화
정화빈 ( Hwabin Jung ),판철호 ( Cheol Ho Pan ),윤원병 ( Won Byong Yoon ) 한국산업식품공학회 2015 산업 식품공학 Vol.19 No.3
The effect of high hydrostatic pressure (HHP) on the physical properties of the starch in buckwheat dough (BD) for noodle application was studied. HHP was applied to the BD at various pressures: at 50 MPa and 415 MPa for 9 min and at 200 MPa for 3 min and 15 min. The microstructure of BD after HHP treatment showed a higher degree of swelling of starch granules as the pressure level and the holding time increased. The starch granules in BD at 415 MPa were ruptured whereas other samples were gelatinized with intact starch granules. The failure tensile distance of BD, the most important quality index of dough for noodle processing, significantly increased at 200 MPa for 15 min (p<0.05). The springiness, adhesiveness, and chewiness of cooked dough were reduced as the pressure level increased, whereas the gumminess, springiness, and chewiness moderately increased as the holding time increased. Our study demonstrated that the noodle dough treated with suitable pressure level and holding time improved not only the process-ability of gluten free dough but also the texture characteristics of cooked noodle product.
고초균에서 발현된 벼 키틴분해효소 ( CH16 ) 의 병원성 곰팡이 생장 저해 효과
손종문(Jong Mun Son),판철호(Cheol Ho Pan),소민영(Min Young So),채영암(Young Am Chae),김수일(Su Il Kim) 한국키틴키토산학회 2001 한국키틴키토산학회지 Vol.6 No.1
N/A To investigate whether or not translational product of rice chitinase cDNA CH16 has antifungal activity, recombinant CH16 was produced in microorganism and its antifungal activity was tested in vitro. The rice chitinase cDNA, CH16, which encodes a basic class I chitinase, was expressed in Bacillus subtilis. To prepare the expression plasmid, CH16 was inserted into the multicloning site of a plasmid, p8A-1, which contains the promoter and signal sequence of Bacillus amylase. The expression cassette consisting of promoter, signal sequence, and ORF of CH16 was subcloned into the shuttle vector, pUBL140, which can be replicated in E. coli as well as B. subtilis. The resulting plasmid was named as pUBLCH16. Expression plasmid was transformed in B. subtilis WB600 deficient in six extracellular proteases and transformant expressed recombinant chitinase, CH16, at the stationary phase. CH16 protein showed the growth inhibition of Fusarium oysporum R2 which was isolated from the root of Rhemannia glutinosa and specifically infected to Rehmannia root.
유색감자 자영 분말을 이용한 쿠키의 항산화활성 및 품질특성
정화빈 ( Hwa Bin Jung ),김지아 ( Ji A Kim ),판철호 ( Cheol Ho Pan ),윤원병 ( Won Byong Yoon ) 한국산업식품공학회 2014 산업 식품공학 Vol.18 No.4
This study was conducted to investigate the quality characteristics of cookies prepared with colored potato (Jayoung) flour having high antioxidant activity. Total phenol, color difference, browning index, textural properties and sensory properties of the cookies were evaluated. Total phenols increased with increasing Ja-young flour contents as well as baking time. In addition, the browning index (BI) increased with increasing proportions of Ja-young flour and baking time. The BI curves were fitted to three different kinetic models: zero order, Weibull, and logistic model. The reaction rate constant, RMSE and R-square parameters were estimated. The logistic model was an appropriate model for describing the browning of cookies with Ja-young flour by baking time based on RMSE and R-square. The color difference decreased significantly as the proportion of Ja-young flour in the blends and baking time increased. The hardness of cookies increased with increasing Ja-young flour in the blends up to 13 minutes of baking. These results suggest that Ja-young flour is a suitable ingredient to develop cookies with high antioxidant activity and novel texture.
유색감자 자영과 홍영을 이용한 제면 특성 및 항산화 활성
홍윤기 ( Yoon Ki Hong ),정화빈 ( Hwabin Jung ),김상민 ( Sang Min Kim ),판철호 ( Cheol Ho Pan ),남정환 ( Jung Hwan Nam ),조지홍 ( Ji Hong Cho ),권오근 ( Oh Keun Kwon ),정진철 ( Jin Cheol Jeong ),이재권 ( Jae Kwon Lee ),윤원병 ( Won 충북대학교 과학교육연구소 2014 과학교육연구논총 Vol.29 No.1
The experiment was conducted to change physical properties and antioxidant activity of flour noodle and boiled flour dough that added coloured potato (Hongyoung and Jayoung) powder(RF and PF) and starch(RSF and PSF). when coloured potato was added to dough, the flavor and texture of its noodle are better than noodle made by wheat flour (WF). The hardness, gumminess and chewiness of dough added coloured potato flour showed higher value than wheat flour dough. The total phenolic compound of dough added coloured potato flour was 3.7-5.8 times higher than boiled wheat flour dough. Additionally, DPPH scavenging acvitity was 1.5-1.6 times higher than boiled weat flour dough. It indicate that manufacturing noodle with coloured potato flour present better quality than wheat flour that was generally used for manufacturing noodle.
Phaeodactylum tricornutum의 (E)-4-Hydroxy-3-methylbut-2-enyl Diphosphate Reductase 유전자의 형질전환
신복규 ( Bok Kyu Shin ),정유진 ( Yu Jin Jung ),김상민 ( Sang Min Kim ),판철호 ( Cheol Ho Pan ) 한국응용생명화학회 2015 Journal of Applied Biological Chemistry (J. Appl. Vol.58 No.3
Phaeodactylum tricornutum is a model diatom that its genomic information and biological tools are well established. In this study, a gene encoding (E)-4-hydroxy-3-methylbut-2-enyl diphosphate reductase (PtHDR), a terminal enzyme of the methylerythritol phosphate pathway regulating chlorophyll and carotenoid biosynthesis, was isolated from P. tricornutum. The isolated gene was cloned into pPha-T1 vector containing fcpA promoter to prepare pPha-T1-HDR plasmid. As a positive control, pPha-T1-eGFP plasmid was constructed with egfp gene. Stable nuclear transformation was carried out with these plasmids by particle bombardment method and zeocin resistant colonies of P. tricornutum were selected on f/2 agar plate. In result, transformation efficiency was evaluated according to the amount of plasmid DNA coated with gold particles. Integration of introduced plasmids was confirmed with genomic DNA of each transformant by polymerase chain reaction. The eGFP fluorescence was visible in the cytoplasm, indicating that eGFP was successively expressed in P. tricornutum system. The transcript level of exogenous Pthdr gene was evaluated with the obtained transformants. The results presented here demonstrated that introduction of Pthdr gene into P. tricornutum chromosome succeeded and expression of PtHDR was enhanced under the fcpA promoter.
김상민(Sang Min Kim),정유진(Yu-Jin Jung),판철호(Cheol-Ho Pan),엄병헌(Byung-Hun Um) 한국식품영양과학회 2010 한국식품영양과학회지 Vol.39 No.5
본 실험에서는 국내에 자생하는 콩과에 속하는 6종의 싸리속 식물의 각 부위별 추출물로부터 총 폴리페놀, 총 플라 보노이드 함량을 측정하였고, DPPH, ABTS 라디칼 소거능을 조사하였다. 폴리페놀성 화합물은 고양싸리의 잎(LR-L) 에 가장 많이 포함되어 있는 것으로 조사되었고, 플라보노이드 화합물은 풀싸리의 지상부(LTi-A)에서 가장 많이 검출 되었다. 이들 시료의 항산화 활성을 조사하기 위해서 DPPH 와 ABTS 라디칼에 대한 소거 활성을 조사해 본 결과 DPPH 라디칼에 대해서는 비타민 C보다 소거 활성이 적은 것으로 나타났지만, ABTS 라디칼에 대해서는 비타민 C보다 더 좋은 소거 활성을 보였다. 이들 활성과 폴리페놀, 플라보노이드 화합물간의 상관관계에서는 폴리페놀만이 양의 상관관계를 보여 폴리페놀의 농도에 따라 라디칼 소거 활성이 증가하는 것을 관찰할 수 있었다. 하지만 플라보노이드 화합물과 라디칼 소거 활성 간에는 뚜렷한 상관관계를 볼 수 없어, 싸리속 식물에서는 플라보노이드 화합물보다는 폴리페놀성 화합물이 항산화에 더욱 중요한 역할을 하는 것으로 판단할 수 있었다. 이러한 결과는 최근 각광을 받고 있는 천연 항산화제의 개발에 있어 싸리속 식물이 천연 항산화제로서 좋은 소재가 될 수 있음을 보여주고 있다. The genus Lespedeza belonging to Leguminosae is an annual or perennial herb, which has been used as a Chinese herbal medicine. Antioxidant activity on DPPH ((1,1)-diphenyl-2-picrylhydrazyl) and ABTS (2,2'-azino-bis(3-ethylenebenzothiazoline-6-sulfonic acid) radicals and the contents of total polyphenol and flavonoid were analyzed with 12 methanol extracts from six Lespedeza genus plants with different plant parts. The highest content of total polyphenol was detected in the leaves of Lespedeza×robusta (194.6 mg GAE/g), while the highest content of total flavonoid existed in the aerial part of Lespedeza thunbergii var. intermedia (47.9 ㎎ QE/g). Generally, the amounts of polyphenol and flavonoid compounds were higher in the leaves than in the stems. The SC?? value of DPPH radical scavenging activity ranged from 10.16 to 90.94 ppm, of which the strongest activity was determined in the leaves of L. robusta. Most ABTS radical scavenging activities from the investigated methanol extracts were higher than that of L-ascorbic acid, implying the excellent antioxidant activity. The radical scavenging activity in this study showed high correlation with the amount of total polyphenol rather than that of total flavonoid. These data suggest that the methanol extracts from these Lespedeza spp. could be potential candidates for natural antioxidants.
김민선 ( Min-seon Kim ),진종범 ( Jong Beom Jin ),이정환 ( Jung Hwan Lee ),안혜숙 ( Hye Suck An ),판철호 ( Cheol-ho Pan ),박진수 ( Jin-soo Park ) 한국응용생명화학회 2021 Journal of Applied Biological Chemistry (J. Appl. Vol.64 No.1
Phytochemicals include plant-derived natural products that promote and improve the human metabolism and physiological activity, and there is a lot of research to find the value of the molecules is in progress. Likewise, we obtained 288 fractions of Capsicum annuum L. extract in less than 20 h using HPLC/SPE/ HPLC coupling experiment through Sepbox system, an effective separation system to search for active substances in natural resources and ensure efficacy and reliability. Therefore, this experiment allowed rapid identification of biologically active molecules from the extract compared to traditional separation processes. Of the above fractions, eight fractions showed the α- glucosidase inhibitory (AGI) activity and subsequent LC-MS analysis revealed one of the active molecules as luteolin 7-Oglucoside. In addition, we proved the increase in AGI activity according to deglycosylation of flavonoid glycoside. Therefore, this study suggests that the Sepbox system can quickly separate and identify active components from plant extract, and is an effective technique for finding new active substances.