동과자 에탄올 추출물이 비알코올성 지방간 세포 모델에 미치는 효과

최준용,김소연,권민정,김균하,주명수,한창우,Choi, Jun-Young,Kim, So-Yeon,Kwun, Min-Jung,Kim, Kyun-Ha,Joo, Myung-Soo,Han, Chang-Woo 대한한방내과학회 2012 大韓韓方內科學會誌 Vol.33 No.4

Objectives : In this study, we investigated the effect and the underlying mechanism of ethanol extract of Benincasa seeds on a cellular model of non-alcoholic fatty liver disease (NAFLD) established by treating HepG2 cells with palmitate. Methods : We evaluated ethanol extract of Benincasa seeds (EEBS) for its hepatic lipid-lowering potential in fatty acid overloaded HepG2 cells. After incubation in palmitate containing media with or without EEBS, intracellular neutral lipid accumulations were quantified by Nile red staining. We also investigated the effect of EEBS on lipogenesis and ${\beta}$-oxidation. $LXR{\alpha}$-dependent SREBP-1c activation, expression of lipogenic genes, and expression of ${\beta}$-oxidation related genes were determined with or without pretreatment of EEBS. Results : EEBS significantly attenuated palmitate-induced intracellular neutral lipid accumulation in HepG2 cells. EEBS suppressed fatty acid synthesis by inhibiting $LXR{\alpha}$-dependent SREBP-1c activation. EEBS also repressed SREBP-1c mediated induction of lipogenic genes, including ACC, FAS, and SCD-1. However, EEBS had no effect on ${\beta}$-oxidation related CPT-1 and $PPAR{\alpha}$ gene expression. Conclusions : Our results suggest that EEBS has an efficacy to decrease hepatic lipid accumulation, and this effect was mediated by inhibiting the $LXR{\alpha}$-SREBP-1c pathway that leads to expression of lipogenic genes and hepatic steatosis. Therefore, the Benincasa seeds may have a potential clinical application for treatment of this chronic liver disease.

두경부암 방사선치료 시 선량 균일도 향상을 위한 Thermoplastic 구강 보상체의 개발

최준용,원영진,박지연,김종원,문봉기,윤형근,문수호,전종병,서태석,Choi, Joon-Yong,Won, Young-Jin,Park, Ji-Yeon,Kim, Jong-Won,Moon, Bong-Ki,Yoon, Hyong-Geun,Moon, Soo-Ho,Jeon, Jong-Byeong,Suh, Tae-Suk 한국의학물리학회 2012 의학물리 Vol.23 No.4

두경부암 방사선치료 시 공동 경계면 및 주변 치료 부위의 선량 균일도 향상을 위하여 조직 등가의 thermoplastic 구강 보상체를 개발하였다. Thermoplastic의 유용성 평가를 위해, 기존에 사용해 오던 치과용 인상재료인 paraffin, alginate, 그리고 putty로 제작한 각 구강 보상체의 물성 및 선량 분포 향상도를 비교하였다. 물성 평가에는 강도 평가(압축 실험, 낙하 실험)와 자연변형도(시간에 따른 체적 변화) 평가를 수행하였으며, 개발한 선량 검증용 팬톰에 삽입한 유리선량계와 Gafchromic EBT2 필름을 이용하여 표면선량, 공동 경계면 선량 및 빔 측면도를 측정하여 전달 선량을 평가하였다. 두 달간 각 구강 보상체의 자연변형도 평가하였을 때, alginate는 수분증발로 최대 80% 체적 변화를 보였으나, thermoplastic을 포함한 나머지 조직 등가 물질은 체적 변화가 3% 미만으로 나타났다. 강도 평가 중 5회 반복한 1.5 m 높이의 자유 낙하실험에서 paraffin은 충격에 의하여 파손이 발생되었으나, thermoplastic은 낙하에 의한 파손이 발생되지 않았으며, 압축 강도 실험에서도 paraffin에 비하여 8배 이상의 높은 힘에서도 파손되지 않았다. 유리선량계를 이용한 선량 검증 결과, 1문 조사 시 조직등가[약 80 HU (Hounsfield Unit)]의 thermoplastic은 동일한 처방 선량 전달 시 약 1,000 HU 이상의 값을 나타내는 putty에 비해 4% 낮은 출력계수(monitor unit) 전달로 약 4.9%의 낮은 표면 선량을 전달하였다. 또한 빔 입사 방향을 기준으로 할 때, 구강 통과 후 경계면의 빔 측면도에서 선량 균일도 평가를 위해 측정한 조사영역 편평도는 air, thermoplastic, putty에서 각각 11.41, 3.98, 4.30으로 나타났다. Thermoplastic 구강 보상체는 조직 등가 물질로 기존에 사용해오던 구강 보상체에 비하여 강도가 높고 물질 변형 확률이 적으며, 구강을 포함을 경계면 및 주변 부위에 균일한 선량 분포를 형성할 수 있으므로 균일한 처방 선량 전달 및 피부 선량 감소가 가능하다. Aquaplast Thermoplastic (AT) is a tissue-equivalent oral compensator that has been developed to improve dose uniformity at the common boundary and around the treated area during radiotherapy in patients with head and neck cancer. In order to assess the usefulness of AT, the degree of improvement in dose distribution and physical properties were compared to those of oral compensators made using paraffin, alginate, and putty, which are materials conventionally used in dental imprinting. To assess the physical properties, strength evaluations (compression and drop evaluations) and natural deformation evaluations (volume change over time) were performed; a Gafchromic EBT2 film and a glass dosimeter inserted into a developed phantom for dose verification were used to measure the common boundary dose and the beam profile to assess the dose delivery. When the natural deformation of the oral compensators was assessed over a two-month period, alginate exhibited a maximum of 80% change in volume from moisture evaporation, while the remaining tissue-equivalent properties, including those of AT, showed a change in volume that was less than 3%. In a free-fall test at a height of 1.5 m (repeated 5 times as a strength evaluation), paraffin was easily damaged by the impact, but AT exhibited no damage from the fall. In compressive strength testing, AT was not destroyed even at 8 times the force needed for paraffin. In dose verification using a glass dosimeter, the results showed that in a single test, the tissue-equivalent (about 80 Hounsfield Units [HU]) AT delivered about 4.9% lower surface dose in terms of delivery of an output coefficient (monitor unit), which was 4% lower than putty and exhibited a value of about 1,000 HU or higher during a dose delivery of the same formulation. In addition, when the incident direction of the beam was used as a reference, the uniformity of the dose, as assessed from the beam profile at the boundary after passing through the oral compensators, was 11.41, 3.98, and 4.30 for air, AT, and putty, respectively. The AT oral compensator had a higher strength and lower probability of material transformation than the oral compensators conventionally used as a tissue-equivalent material, and a uniform dose distribution was successfully formed at the boundary and surrounding area including the mouth. It was also possible to deliver a uniformly formulated dose and reduce the skin dose delivery.

휴대용 폐기능 검사기의 재현성과 기대치에 관한 연구

최준용,정희재,이건영,정승연,황준호,이형구,정승기,Choi, Jun-Yong,Jung, Hee-Jae,Lee, Kun-Young,Jeong, Seung-Yeon,Hwang, Joon-Ho,Rhee, Hyung-Koo,Jung, Sung-Ki 대한한방내과학회 2005 大韓韓方內科學會誌 Vol.26 No.3

Objectives : The object of this study was to evaluate repeatability of portable spirometry and to determine an appropriate equation for predicted value. Materials and Methods : The subjects were 76 men and 45 women recruited from among Kyunghee University oriental medical students. Portable spirometry was performed via the procedure recommended in ATS guidelines. Repeatability of spirometry was checked against ATS repeatability standards and NHLEP QC grade. Percentage of predicted value of non-smokers was calculated through a Caucasian population-based regression equation(by Morris et al.) and Korean population based regression equation(by Kim et al.). These were compared. Result : 91.7% of subjects matched repeatability standards of ATS and 95% of subjects got interpretable NHLEP QC grades of A, B, and C. Mean percentage of predicted value by Morris et al. were as follows: Mean of FVC% was 90.86, FEV1% was 96.66, FEV1/FVC% was 107.48. Mean percentage of predicted value by Kim et al. were as follows. Mean FVC% was 92.38, FEV1% was 89.77, FEV1/FVC% was 96.95. Conclusions : Results of this study show that more than 90% of subjects meet repeatability criteria. This supports a role for the portable spirometer as a primary oriental medical office tool. Further, results are a verify that they Korean population-based predicted value equation is a more appropriate standard than the Caucasian population-based one, but a still more accurate standard is needed for the Korean adult population.

두시하태독법의 Th2 분화 조절을 통한 알레르기 비염 완화 효과

최준용,안상현,김기봉,Choi, Jun-yong,Ahn, Sang-hyun,Kim, Ki-bong 대한한방내과학회 2017 大韓韓方內科學會誌 Vol.38 No.4

Objectives: This study investigated the effects of Douchi Hataedock on Th2-skewed conditions to control allergic rhinitis. Methods: NC/Nga mice were divided into three groups: 10 mice were assigned to the control group (CTRL; no treatment), 10 mice to allergic rhinitis-induced (ARE) without treatment group, and 10 mice to the allergic rhinitis-induced (FGT) after Douchi Hataedock treatment group. The 3-week-old mice of the FGT group were given one 10 mg/kg dose of Douchi Hataedock extract and resensitized to allergic antigens at weeks four, five, and six. Allergic rhinitis was induced primarily in mice nasal cavities for five days after one week of final sensitization. The second induction used the same method one week after the first induction was completed. After one week, the nasal mucosal tissues of each group were observed. Immunohistochemical staining for IL-4, STAT6, CD40, $Fc{\varepsilon}RI$, substance P, MMP-9, $NF-{\kappa}B$ p65, p-IkB, and iNOS in the nasal mucosa was also performed. Results: The FGT group had less respiratory epithelial damage and less mucin secretion in goblet cells than the ARE group and showed a 62% decrease in IL-4, 85% decrease in STAT6, 71% decrease in CD40, 69% decrease in $Fc{\varepsilon}RI$, 43% decrease in substance P, 49% decrease in MMP-9, 43% decrease in NF-kB p65, 38% decrease in p-IkB, and 73% decrease in iNOS compared to the ARE group. Conclusions: Douchi Hataedock lessens inflammation in epithelial and goblet cells and reduces inflammatory mediator secretion in a mouse allergic rhinitis model.

청상보하탕(淸上補下湯)의 기관지천식환자에 대한 스테로이드 절약효과

최준용,이재성,주창엽,정희재,이형구,정승기,황우석,Choi Jun-Yong,Lee Jae-Sung,Ju Chang-Yeop,Jung Hee-Jae,Rhee Hyung-Koo,Jung Sung-Ki,Hwang Woo-Suck 대한한방내과학회 2003 大韓韓方內科學會誌 Vol.24 No.1

Background : In recent years, the glucocorticoid hormone has become a fundamental medication for asthma. However, a long period of hormone administration will result. in general. side effects on many body parts as well as hormone dependence, which has become a serious problem for western physicians. Objectives : We aimed to identify the clinical effects of Cheongsanghoha-tang and the steroid sparing effects of Cheongsanghoha-tang on. Materials and Methods : A subject group consists of 36 asthmatics who had been treated with Cheongsanghoha-tang for four weeks. Cheongsanghoha-tang is a herbal decoction, which has been used of the traditional therapeutic agent of asthma. PFT, QLQAKA, blood eosinophil, serum IgE, Serum IL-4. IL-5, IFN-${\gamma}$ were checked before and 4weeks after the treatment. Results : The only FVC% in ICSG among asthmatic patients was increased significantly compared to NICSG. Treatment of Cheongsanghoha-tang for four weeks resulted in significant increase in QLQAKA. The NICSG treated with Cheongsangboha-tang for four weeks were no significant difference in the blood eosinophil, serum IgE, IL-4 and IL-5. The PFT and QLQAKA in NICSG were increased significantly after 4 weeks treatment. But the serum IFN-${\gamma}$ in NICSG was decreased significantly after 4 weeks treatment. Discontinuation of treatment with inhaled corticosteroid in ICSG resulted in insignificant changes in PFT, the blood eosinophil, the serum IgE, IL-4, IL-5 and IFN-${\gamma}$ and significant increase in QLQAKA. As a result. 8 of 13 cases were cured with hormones completely and the rest of ICSG reduced the dose of ICS. Conclusions : This study shows that Cheongsanghoha-tang has the effects on the improvement of pulmonary function and cures asthmatic patients. These findings demonstrate that Cheongsanghoha-tang has the steroid sparing effect. Some satisfactory therapeutic results have been obtained in treating hormone-dependent asthma by Cheongsanghoha-tang. However. the concept and mechanism of hormone-dependent asthma have not been fully defined yet, and the standard for judging therapeutic effects have not been established. Obviously further researches concerning all these are still necessary.

본 임상시험을 대비한 천식 침치료 무작위 대조군 예비 임상시험 프로토콜

최준용 ( Jung Yong Choi ),오달석 ( Dal Seok Oh ),노영래 ( Young Lae Roh ),정희재 ( Hee Jae Jung ),정승기 ( Sung Ki Jung ),최선미 ( Sun Mi Choi ) 대한경락경혈학회 2008 Korean Journal of Acupuncture Vol.25 No.3

배경: 최근까지 이루어진 천식의 침치료 무작위 대조군 연구들에 의한 결과는 뚜렷한 효과를 보여주지 못하고 있다. 이러한 요인의 하나로 적절한 피험자 수를 정하기 위한 예비 임상시험의 부재가 거론되고 있다. 따라서 천 식에 대한 침치료의 본 임상시험에 앞서 이의 실행 가능성 및 규모를 예측하는 예비임상시험이 필요한 상황이다. 목적: 예비임상시험을 통해 서양의학적 치료를 받고 있는 천식환자들에 대한 침치료의 부가적 효과를 검증하기 위한 본 임상시험의 실현 가능성을 예측하기 위함. 방법: 본 시험은 무작위 단일 맹검 대조군 평행 설계 예비 임상시험이다. 1차 평가변수는 최대 호기량 검사기로 집에서 아침, 저녁으로 매일 측정한 수치로 구해진 최대 호기 유속 일중 변동률의 1주간 평균치 변화이며 2차 평 가변수는 의료기관에서 시행하는 폐기능 검사, 기준시점 호흡곤란 지수, 호흡곤란 변화에 대한 지수, 천식환자들 의 삶의 질 평가를 위한 설문 등이다. 피험자 수는 총 45명으로 선정 및 제외기준을 통해서 참여하게 되며 1주간 의 최대 호기 유속 기저치 평가 기간을 거친 후 진짜침 처치 군, 가짜침 처치 군, 대기군 세 군에 각각 무작위로 15명씩 할당된다. 모든 피험자에게 기존의 천식관련 약물의 사용을 허용하며, 진짜침 처치군 및 가짜침 처치군은 주 3회 4주간 해당 처치를 받고 2주 후에 각 평가 항목에 대하여 추적, 관찰하게 된다. 대기군은 기존의 천식관리를 유지하면서 2주마다 방문하여 총 6주간 평가를 받고 진짜침 처치를 4주간 받게 된다. 고찰: 본 연구는 국내에서 최초로 이루어지는 천식에 대한 침 무작위 대조군 예비 임상시험으로 향후 적절한 피 험자수와 실행가능성을 예측할 수 있으리라 기대된다.

전자 빔 리소그래피를 이용한 주사기법에 따른 패턴형상에 관한 연구

최준용(Jun Yong Choi),오세규(Se Kyu Oh),김승재(Seoung Jae Kim),김동환(Dong Hwan Kim) 한국생산제조학회 2008 한국생산제조시스템학회 학술발표대회 논문집 Vol.2008 No.5

In a electron beam lithography, pattern shapes are dependent on the scanning method. In this work, four types of scanning methods are implemented and their characteristics are investigated. For a 11x11um pattern, a Zigzag scanning method proves a precise pattern generation. The other ways such as SEM scanning and swirl in-out scanning method result in some distorted pattern shape.

Developing a Framework for Pandemic COVID-19 Vaccine Allocation: a Modified Delphi Consensus Study in Korea

Choi Min Joo,Choi Won Suk,Seong Hye,최준용,Kim Jong-Hyun,Kim Yae-Jean,Cho Eun Young,김동현,Park Hyesook,Lee Heeyoung,Kim Nam Joong,Song Joon Young,Cheong Hee Jin,Kim Sang Il,Peck Kyong Ran 대한의학회 2021 Journal of Korean medical science Vol.36 No.23

Background: This study presents a framework for determining the allocation and distribution of the limited amount of vaccines against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). Methods: After analyzing the pandemic strategies of the major organizations and countries and with a literature review conducted by a core panel, a modified Delphi survey was administered to 13 experts in the fields of vaccination, infectious disease, and public health in the Republic of Korea. The following topics were discussed: 1) identifying the objectives of the vaccination strategy, 2) identifying allocation criteria, and 3) establishing a step-by-step vaccination framework and prioritization strategy based on the allocation criteria. Two rounds of surveys were conducted for each topic, with a structured questionnaire provided via e-mail in the first round. After analyzing the responses, a meeting with the experts was held to obtain consensus on how to prioritize the population groups. Results: The first objective of the vaccination strategy was maintenance of the integrity of the healthcare system and critical infrastructure, followed by reduction of morbidity and mortality and reduction of community transmission. In the initial phase, older adult residents in care homes, high-risk health and social care workers, and personal support workers who work in direct contact with coronavirus disease 2019 (COVID-19) patients would be prioritized. Expansion of vaccine supply would allow immunization of older adults not included in phase 1, followed by healthcare workers not previously included and individuals with comorbidities. Further widespread vaccine supply would ensure availability to the extended adult age groups (50–64 years old), critical workers outside the health sector, residents who cannot socially distance, and, eventually, the remaining populations. Conclusion: This survey provides the much needed insight into the decision-making process for vaccine allocation at the national level. However, flexibility in adapting to strategies will be essential, as new information is constantly emerging.

자하거(紫河車) 약침의 기관지 천식 치료 가능성에 대한 소고(小考)

최준용 ( Jun Young Choi ),오달석 ( Dal Seok Oh ),정희재 ( Hee Jae Jung ),최선미 ( Sun Mi Choi ),정승기 ( Sung Ki Jung ) 대한경락경혈학회 2008 Korean Journal of Acupuncture Vol.25 No.2

Objectives: The aim of this study was to find out some rationales for using Hominis placental extract(HPE) pharmaco-acupuncture on asthmatic patients, and to suggest an appropriate clinical research of HPE pharmaco-acupuncture on asthma. Methods: Articles regarding immunologic experiments about HPE and clinical reports about HPE-contained pharmaco-acupuncture on asthmatic patients were reviewed by on-line and off-line searching. Results and Conclusions: HPE is suggested to have several pharmacological effects on allergic diseases including asthma as well as autoimmune diseases. There are some observational studies of HPE-contained pharmaco-acupuncture on asthmatic patients. However, the designs of these studies are not sufficient to investigate the exact effect of HPE pharmaco-acupuncture on asthma. To investigate HPE pharmaco- acupuncture as a novel asthma therapy, it will be needed to perform further experiments about HPE`s pharmacological mechanism on asthma and to try further advanced clinical researches such as well-designed randomized controlled trial of HPE pharmaco-acupuncture.

대한한의학회지 및 대한한의학회 산하 정회원 학회의 학술진흥재단 등재지에 대한 천식 임상연구의 체계적 고찰

최준용(Jun-Yong Choi),방지현(Ji-Hyung Bang),심봉암(Feng-Yan Shen),정희재(Hee-Jae Jung),정승기(Sung-Ki Jung) 대한한의학회 2010 대한한의학회지 Vol.31 No.1