부항요법 후 적외선 체열촬영을 통한 관찰

최윤희,조정훈,장준복,이경섭,Choi, Yun-Hui,Cho, Jung-Hoon,Jang, Jun-Bock,Lee, Kyung-Sub 대한한방체열의학회 2005 대한한방체열의학회지 Vol.4 No.1

Thermal change of thermogram can be induced by heat, drugs, diseases, stress, treatments and so on. Because they changed blood circulation of body surface. Negative treatment have been used in oriental medicine to decrease pain and to purify fluids and it can also change blood circulation of body surface. The purpose of this study was to observe the detail changes of temperature after negative treatment and to know the differences according to the negative pressures by thermography. This study was carried for 5 days and thermogram was operated 15 times. The patient had been treated on the back with electromotive type negative treatment and the treated sites was devides into 4 parts with different negative pressure. The results was that the temperature of all parts rised after treatment and the highest point was 10 minutes after treatment. The differences according to the negative pressures was that the temperature of the 55mmHg part rised particularly than the others and it continued until 40 minutes after treatment. There were no differences between 30mmHg and 40mmHg part. The temperature of 50mmHg part were low than the others during all thermogram.

소양인, 소음인 월경통 환자의 체표온도 비교

최윤희,조정훈,장준복,이경섭,Choi, Yun-Hui,Cho, Jung-Hoon,Jang, Jun-Bock,Lee, Kyung-Sub 대한한방체열의학회 2006 대한한방체열의학회지 Vol.5 No.1

Dysmenorrhea is one of the most common gynecologic disorders in menstruating women. Dysmenorrhea refers to the pain appearing in the lower abdomen and lower back before. after or during menstruation. In oriental medicine. the causes of dysmenorrhea have been recognized as something penetrated such as qi. blood. and pathologic factors. The purpose of this study was to observe the difference in the temperature between the Soyangin dysmenorrhea patients group and the Soeumin dysmenorrhea patients group by thermography. We observed the 55 dysmenorrhea patients that visited ** Oriental Medicine center from July 19 to August 27. 2004. We diagnosed the constitution by QSCCII and the body temperature was assessed by DITI. Thermographic measurements were performed on 3 areas(Chon-jung(CV17), Chung-wan (CV12), Kwan-won(CV4)). In this study we observed that the temperature of Chon-jung(CV17) is the highest and Chung-wan (CV12) is higher than Kwan-won(CV4). The temperature of Soeumin group is higher than that of Soyangin group in all areas and there is a significant difference in 3 area temperature between Soeumin group and Soyangin group.

만성 본드 흡입 환자에게 발생한 호흡부전 1례

최윤희,이동훈,어은경,Choi, Yoon-Hee,Lee, Dong-Hoon,Eo, Eun-Kyung 대한임상독성학회 2005 대한임상독성학회지 Vol.3 No.1

Toluene is an aromatic hydrocarbon found in glues, cements, and solvents. It is known to be toxic to the nervous system, hematopoietic system, and causes acid-base and electrolyte disorders. Acute respiratory failure with hypokalemia and rhabdomyolysis with acute renal failure should be considered as potential events in protracted glue sniffing. We reported the case of 26-year-old woman was admitted to the emergency department with the development of respiratory failure and altered mentality due to hypokalemia after chronic glue sniffing. She was weaned from the ventilator 3 days later after potassium and sodium bicarbonate replacement and was discharged without respiratory symptoms and other complications.

대학도서관 사서 역량개발 방향 탐색을 위한 직원교육 현황분석

최윤희,정유경,Choi, Yoonhee,Jeong, Yoo Kyung 한국도서관정보학회 2021 한국도서관정보학회지 Vol.52 No.1

본 연구는 사서의 자기계발 관심영역을 알아보기 위해 대학도서관 사서가 한 해 동안 수강한 교육이수내역을 바탕으로 사서 계속교육의 현황을 파악하고자 하였다. 이를 위해 한국교육학술정보원에서 실시한 대학도서관 통계의 직원 교육훈련 항목을 활용하였으며, 사서들의 교육이수내역 30,404건을 대상으로 직원교육의 핵심 키워드를 부여하고 분류하는 작업을 수행하여 사서의 선호 교육주제, 대학 규모별, 지역별, 월별 관심 있는 교육주제 등의 현황을 살펴보았다. 분석결과, 전체 수강강좌의 주제 중 가장 많이 나타난 직원교육 주제는 'DB이용'과 '자료목록'으로 나타났으며, 대학 유형(대학/전문대학)과 규모, 지역 등에서 사서의 교육훈련 관심영역 간의 차이를 확인하였다. 또한, 사서들에게 요구되는 다양한 역량에 비해 '정보자원 및 평가'와 관련된 주제 영역에 관심이 치우쳐져 있음을 알 수 있었다. 본 연구는 대학도서관 사서들의 교육이수내역 전수조사를 통해 교육의 주제영역에 따른 현황을 파악한 것으로, 향후 대학도서관 사서들의 실질적인 관심사를 반영하고 보다 체계적인 교육과정을 개발하는데 기초자료로 활용될 수 있을 것이다. This study aims to analyze the current status of continuing education for academic librarians based on the records of education. For this purpose, the study investigated the academic librarian's preferences for the topics of continuing education in terms of regional and monthly differences using the 30,404 education records in university and college library statistics of Korean Education and Research Information Service (KERIS). The results shows that 'academic database system' and 'cataloging' were the most preferred topics, and there were topical differences between the academic librarians in university and college.

4종류의 동해 방지제에 침지 한 진주조개, Pinctada fucata martensii와 참굴, Crassostrea gigas 담륜자의 생존율

최윤희,장영진,CHOI Youn Hee,CHANG Young Jin 한국수산과학회 1999 한국수산과학회지 Vol.32 No.4

Experiments were carried out to evaluate the tolerance of trochophores for pearl oyster, Pinctada fucata martensii and Pacific oyster, Crassostrea gigas using different concentrations of cryoprotectants : dimethyl sulfoxide (DMSO), ethylene glycol, glycerol and 1,2-propanediol. Each cryoprotectant with different concentrations was exposed for 5, 10, 15 and 20 minutes of immersion time. Survival rates were increased with decreased concentrations of cryoprotectant and decreased immersion time, and these differed from types of cryoprotectant. Survival rates of Pacific oyster trochophores were higher in DMSO and ethylene glycol, while those of pearl oyster trochophores were higher in glycerol and 1,2-propanediol. In case of trochophores from Pacific oyster, when 0.2 M sucrose was added in each cryoprotectant the survival rates were increased significantly. 조개류 유생의 동해방지제 독성에 대한 내성을 평가하기 위해 진주조개, Pinctada fucata martensii와 참굴, Crassostrea gigas의 담륜자를 4종류의 동해방지제에 침지하여 생존율을 조사하였다. 실험에 사용된 동해방지제는 각각 0.01$\~$2.0 M의 DMSO, ethylene glycol, glycerol 과 1,2-propanediol이었으며, 침지시간은 5$\~$20분으로 설정하였다. 모든 동해방지제에 진주조개와 참굴 담륜자를 침지하였을 때, 농도가 낮을수록 침지시간이 짧을수록 생존율이 높아지는 경향을 보였으며, 동해방지제의 종류에 따라 담륜자의 생존율이 다르게 나타났다. DMSO와 ethylene glycol에서는 참굴 담륜자의 생존율이, glycerol과 1,2-propanediol에서는 진주조개 담륜자의 생존율이 상대적으로 높게 나타났다. 그러나 참굴에서는 4종류의 동해방지제에 0.2 M sucrose를 첨가하였을 때 생존율이 유의하게 높아졌다.

한국산 무우로부터 Cationic isoperoxidase 의 정제 및 특성규명에 관한 연구

최윤희,박종훈,김승수 ( Yun Hi Choi,Jong Hoon Park,Soung Soo Kim ) 생화학분자생물학회 1989 BMB Reports Vol.22 No.1

A cationic isoperoxidase, designated C₃, was isolated from Korean-radish (Raphanus savatis L.) root. Purification of the enzyme was accomplished by CM-cellulose chromatography and Sephadex G-100 gel filtration. The enzyme was a glycoprotein and its molecular weight was approximately 44,000 as determined by SDS-PAGE and Sephadex G-150 gel filtration. The optimal pH of the purified enzyme was 6.0 for guaiacol and 5.0 for H₂O₂. The Km values for guaiacol and H₂O₂ were 5.6 mM and 0.77 mM, respectively. The Km values against several naturally occurring phenolic compounds were also determined. In comparison with the anionic isoperoxidase A₂ which was previously purified from the same source, C₃ had very high Km values for scopoletin and ferulic acid.

영어 뉴스와 자연발화에 나타나는 고성조 피치액센트의 차이점

최윤희,이주경,Choi, Yun-Hui,Lee, Joo-Kyeong 한국음성학회 2005 음성과학 Vol.12 No.2

This paper argues that news speech entails a distinct intonational pattern from natural speech, effectively reflecting that it primarily focuses on providing new information. We conducted a phonetic experiment to compare the tonal contours between news speech and natural speech, examining the distributions of pitch accents and the overall pitch ranges. We utilized 70 American Press (AP) radio news utterances and 70 natural utterances extracted from TV dramas. Results show that news speech involves 3.38 H*'s (including L+H* and !H*) within an intonational phrase (IP) or intermediate phrase (ip) whereas natural speech, 1.8 in average. The number of IP/ip's per sentence is 3 in news speech, which is shown in the highest rate of 32.07% of the news speech, but it is merely 1, taking up the highest 41.42% in natural speech. Next, declination tends to be prevented in news speech, and the pitch range is much greater in news speech than in natural speech. Finally, a secondary stress syllable is comparatively frequently given a pitch accent in news speech, explicitly distinct from natural speech. These results can be interpreted as stating that news has the particular purpose of providing new information; every content word tends to be given a H* or its related pitch accent like L+H* or !H* because news speech assumes that every word conveys new information. This definitely brings about more IP/ip's per sentence due to a human physiological constraint; that is, more H*'s will cause more respiratory breaks. Also, greater pitch ranges and pitch accents imposed on secondary stress may be attributed to exaggerating new information.

Purification and Characterization of a Cationic Isoperoxidase from Korean-Radish Root

최윤희,박종훈,김승수,Choi, Yun-Hi,Park, Jong-Hoon,Kim, Soung-Soo 생화학분자생물학회 1989 한국생화학회지 Vol.22 No.1

한국산 무우로부터 cationic isoperoxidase $C_3$를 80% $(NH_4)_2SO_4$ 침전, CM-cellulose chromatography, Sephadex G-100 gel filtration 방법 등을 이용하여 전기이동상에서 하나의 띠로 정제하였다. 정제된 효소는 glycoprotein 이었으며 분자량은 SDS-PAGE와 Sephadex G-150 gel filtration에 의하여 약 44,000으로 나타났다. 정제된 isoperoxidase $C_3$는 guaiacol을 기질로 사용한 경우에 최적 pH가 6.0이었으며, guaiacol과 $H_2O_2$에 대한 Km값은 각각 5.6 mM와 0.77 mM이었다. 여러가지 phenolic compound들에 대한 기질 선호도를 조사한 결과 isoperoxidase $C_3$는 scopoletin, esculetin 그리고 ferulic acid에 대해 높은 Km값을 가지며 anionic isoperoxidase $A_2$와 비교해 볼 때 scopoletin과 ferulic acid에 대하여 기질 친화도가 크게 달랐다. A cationic isoperoxidase, designated $C_3$, was isolated from Korean-radish (Raphanus savatis L.) root. Purification of the enzyme was accomplished by CM-cellulose chromatography and Sephadex G-100 gel filtration. The enzyme was a glycoprotein and its molecular weight was approximately 44,000 as determined by SDS-PAGE and Sephadex G-150 gel filtration. The optimal pH of the purified enzyme was 6.0 for guaiacol and 5.0 for $H_2O_2$. The Km values for guaiacol and $H_2O_2$ were 5.6 mM and 0.77 mM, respectively. The Km values against several naturally occurring phenolic compounds were also determined. In comparison with the anionic isoperoxidase $A_2$ which was previously purified from the same source, $C_3$ had very high Km values for scopoletin and ferulic acid.

안와섬유모세포의 지방세포 분화에 대한 혼합 갱글리오사이드의 영향

최윤희,조은형,국경훈,Youn-Hee Choi,Eun Hyung Cho,Koung Hoon Kook 대한안과학회 2011 대한안과학회지 Vol.52 No.3

Purpose: To investigate the role of gangliosides in the differentiation of orbital fibroblasts into adipocytes, a component in the pathogenesis of Graves’ ophthalmopathy. Methods: Orbital tissues were obtained during orbital surgery for subjects without Graves’ ophthalmopathy or other inflammatory orbital disease, and orbital fibroblasts were primarily cultured from each obtained tissue. Morphological examination of orbital fibroblasts was performed after treatment with commercially available gangliosides mixture (Gmix) comprised of several subtypes. To determine the effect of Gmix on the differentiation of orbital fibroblasts into adipocytes and the differentiation-related genes, Oil Red-O staining and RT-PCR were performed. Results: The treatment with Gmix induced the morphological changes, which at least in part were explained with the differentiation of orbital fibroblasts into adipocytes in accordance with the increase of mRNA level of genes known to be related to adipogenesis, whereas dermal fibroblasts and preadipocytes were irresponsive to the same treatment. Conclusions: The results from the present study suggest gangliosides may have a role in pathologic mechanisms of Graves’ ophthalmopathy by the induction of differentiation of orbital fibroblasts into adipocytes. J Korean Ophthalmol Soc 2011;52(3):338-344

다진생강의 저장성 증진에 관한 연구

최윤희,이상복,김명숙 ( Yoon Hee Choi,Sang Bok Lee,Myeong sook Kim ) 한국응용생명화학회 1997 Applied Biological Chemistry (Appl Biol Chem) Vol.40 No.2

To improve the quality and prolongation of chopped ginger, they were treated with NaCl, citric acid, ascorbic acid alone or combination of them at 5℃. The browning and stink of chopped ginger were prevented by adding 10% NaCl, 0.5∼2.0% citric acid, but the control became severely browned and deteriorated after 1 month storage. The application of 0.5% citric acid maintained citrine color and decreased the activity of polyphenol oxidase(PPO) more significantly compared to 5.0% NaCl or 0.5% ascorbic acid. Ascorbic acid treatment become browned at 1.0∼2.0% and easily deteriorated compared to citric acid. Citric acid(0.5%) or ascorbic acid (0.5%) treatment was largely variation of pH compared to NaCl(5.0%)+citric acid(0.5%) or NaCl(5.0%)+ascorbic acid(0.5%) treatment during 8 month storage. The activity of PPO in NaCl(5.0%)+ascorbic acid(0.5%) treatment was elevated after 4 month storage. In chopped ginger, NaCl(5.0%)+citric acid(0.5%)+ascorbic acid(0.25%) treatment was more effective than 5.0%+0.1%+0.1%, 5.0%+0.5%+0.1% during 12 month storage.