유당 자화 효모간의 원형질체 융합

최원기,전순배,이용규,배석,이진종,이향,Choi, Won-Ki,Chun, Soon-Bai,Lee, Yong-Kyu,Bai, Suk,Lee, Jin-Jong,Lee, Hyang 한국미생물학회 1988 미생물학회지 Vol.26 No.3

유당함유 울질로부터 알코올을 생산하기 위해 Saccharomyces cerevisiae X 2180-1 A, Candida pseudotropicalis ATCC 8619와 CBS 607 사이에 속간 및 종내 원형질체 융합을 시도하였다. S. cerevisiae X2180-1A(ade rho)와 C. pseudotropicalis CBS 607 (his met)간의 속간 융합율은 $1.0\times 10^{-5}$ C. pseudotropicalis ATCC 8619와 CBS 607 사이의 종내 융합율은 $3.9\times 10^{-6}$ - $1.0\times 10^{-4}$ 이었다. 이는 bovme serum albumin, myoinositol 그리고 ergosterol을 처리하지 않고 얻어진 원형질체 융합율과 비교해서 약 2-3.5배의 증가를 보여 이들 화합물이 속간 및 종내 융합을 증진시킨 것으로 사료된다. DNA 함량, 핵염색, UV 조사에 따른 생존력 비교 그리고 형질분리 후 교잡형 출현 등을 통해서 속간 (S. cerevisiae와 C. pseudotropicalis)과 종내 ( C psemdotropica/is strains) 융합체에서 핵융합이 일어난 것을 알 수 있었다. 또한 양친형과 비교해서 알코올 생성능이 증가된 종내 융합체를 얻었다. Intergeneric or intraspecific protoplast fusion between Saccharomyces cerevisiae X-2180-1A, Candida pseudotropicalis ATCC 8619 and CBS 607 was attempted to produce ethanol from lactose containing materials. Teh intergeneric fusion frequency between Saccharomyces cerevisiae X-2180-1A (ade rho) and Candida pseudotropicalis CBS 607 (his met) was $1.0\times 10^{-5}$. These values exhibited approximately 2-3.5 fold increase when compared with fusion frequency obtained without the treatment of bovine serum albumin, myoinositol and ergosterol, suggesting that these compounds may improve intergeneric of intraspecific protoplast fusion. Nuclear fusion appears to occur in fusants between intergenera(S. cerevisiae+C. pseudotropicalis) and intraspecies (C. pseudotropicalis strains) as strongly suggested by DNA content, nuclear staining, comparison of survival rate to UV light and isolation of recombinants after mitotic segragation. It was also found that alcohol production from intraspecific hybrids was somewhat increased when compared with that from their parents.

부정치과진료 수진실태에 관한 조사연구

최원기,신승철 ( Won Ky Choi,Seung Chul Shin ) 대한예방치과·구강보건학회 1993 大韓口腔保健學會誌 Vol.17 No.2

동일 체적의 건물 형상에 따른 에너지 소비량 패턴에 대한 분석 연구

최원기,김헌중,서승직,Choi, Won-Ki,Kim, Heon-Joong,Suh, Seung-Jik 한국태양에너지학회 2007 한국태양에너지학회 논문집 Vol.27 No.2

This study was focused on the establishment of a fundamental DB(database) that is available in the building design process, so we performed the simulation analysis about the energy consumption on the various same volume buildings. Because energy consumption in building is affected by the exterior surface area, the ratio of long/short length in surface and the adjacent internal surface area etc.. For these purpose, we assumed the unit module and made a constructable 16 model buildings which are composed of the 16 unit modules. Then we analyzed the simulation using the TRNSYS 16 and the Seoul weather data. In results, energy consumption in building is more reduced that in case of the smaller exterior surface area, the lower stories building and the larger adjacent surface area etc.. Further study is to be required the sensitivity analysis on the various weather conditions, building shapes and window area etc..

고단열 커튼월 핵심 설계 원리에 관한 연구

최원기,이수열,김선형,김성범,김영탁,김진희,Choi, Won-Ki,Lee, Soo-Yeol,Kim, Sun-Hyung,Kim, Sung-Bum,Kim, Young-Tag,Kim, Jin-Hee 한국토지주택공사 토지주택연구원 2021 LHI journal of land, housing, and urban affairs Vol.12 No.1

최근 신축되는 업무용 건물 가운데 커튼월 구조를 갖는 건물의 비중은 갈수록 증가하는 추세에 있다. 그러나 대부분의 커튼월 구조는 알루미늄 프레임과 고성능 창유리로 구성되어, 일반 벽체와 같은 고단열 구조를 갖는데 한계가 있다. 그러나 Low-e 유리와 진공유리의 보급 그리고 다양한 열교차단재의 개발 등으로 커튼월의 단열값도 크게 향상되고 있다. 이에 본 논문에서는 커튼월 프레임의 단열값을 향상시키는 핵심 설계 원리를 통해 제로에너지 건물에도 적합한 수준의 고단열 커튼월 시스템을 개발하는데 목적이 있으며, 대표적인 핵심 기술을 요약하면 다음과 같다. 알루미늄의 열교차단은 최소 2번 이상 구현을 통해 19%의 성능 향상을 도모하고, 창유리 결합 부위에 있어 가능한 한 많은 다중 격실을 갖도록 설계해 최소 25% 이상의 성능 향상을 기대할 수 있다. 또한 상대적으로 구조 및 내화성능이 우수한 폴리아미드를 적용할 경우 이중압출 기술을 활용하여 유리 시공성을 향상시키고 내부 대류 열손실을 최소화하는 설계를 수행할 경우 추가적으로 4% 이상의 성능 향상을 기대할 수 있다. 본 설계 원리를 활용하여 커튼월을 설계할 경우 보다 향상된 단열성능을 구현할 수 있을 것이고, 이러한 고단열 커튼월이 시장에 보급 활성화될 경우 업무용 건물의 에너지 절감과 제로에너지 건축물 구현에 크게 기여할 수 있을 것으로 판단된다. As the proportion of newly built office buildings with curtain-walls has increased so has the need to improve the thermal performance of curtain-walls. Most curtain-wall structures are composed of aluminum frames and high-performance glazing making them more difficult to achieve higher insulation performance levels compared to typical non-glazed insulated walls. However with the growing use of low-e and vacuum glass and the development of various thermal breaks, the U-value of curtain-walls have greatly improved. This paper presents the findings of a research project into a high-performance curtain-wall system suitable for zero-energy buildings through the core design principle of a thermally improved curtain-wall frame. Typical core technologies are summarized as follows. The thermal performance of an aluminum frame curtain-wall can be improved by 19% when a thermal break is implemented at least two times over the frame's section and it can be improved by 25% if the inner space of the glazing pocket is divided into multiple compartments. In addition, the use of polyamide, which has excellent structure and fire resistance properties, can further improve thermal performance by more than 4%. This material can improve glazing workability and minimize internal convective heat loss by utilizing double extrusion technology. Higher insulation performance can be realized when designing a curtain-wall using these design principles which can contribute to energy savings towards the realization of zero-energy buildings.

에코 필름을 이용한 성능 가변 창호 기술 - 스위치 윈도우 시스템

최원기(Choi, Won-Ki),류성룡(Ryu, Seong-Ryong),최현중(Choi, Hyun-Joong),최경석(Choi, Kyung-Suk) 대한건축학회 2023 대한건축학회 학술발표대회 논문집 Vol.43 No.2

In this study, eco-film was applied to windows to verify the possibility of SWITCH Window System. As a result of theoretical analysis, the insulation performance was improved by 20 % and the SHGC performance by more than 50 %. In the physical test results, the insulation performance was improved by 0.27 W/㎡K and the SHGC by more than 0.1. And as a result of simulation analysis for small stores, it was possible to reduce cooling energy by about 27 % in the case of south facing and about 37 % in the case of west facing. As a result of the above analysis, it is expected that if various SWITCH Window System applied with eco-film, a high-functional film, can be used in various buildings all year round, and will have a great effect on building energy saving.

광학현미경 In Situ Hybridization에 의한 Viral RNA 증명

최원기(Won-Gi Choi),주경웅(Keng-Woong Joo),김석홍(Suk-Hong Kim) 대한의생명과학회 1996 Biomedical Science Letters Vol.2 No.2

토끼 출혈증 바이러스에 감염된 조직을 10% 포르말린 고정, 파라핀 블록으로 보관했된 것으로 표본을 만들고 biotin 표지된 올리고뉴클레오티드 probe를 사용하는 in situ hybridization 기법으로 viral RNA를 조사하였다. in situ hybridization 기법은 핵산을 규명하는 다른 방법들에 비하여 신속하고 특이성인 높은 기법으로 모든 과정이 MicroProbe™ capillary action system에서 1-2시간 이내에 완료된다. Viral RNA는 간세포의 세포질과 신장의 피질에서 주로 관찰되었으나, 폐조직과 신장의 수질에서는 부분적으로 적색신호가 보였다. 비록 기술적인 한계를 가지고 있지만 다른 핵산 진단방법 보다 많은 장점을 가지고 있어 조직 병리학적으로 바이러스 진단하는데 하나의 독특한 기법으로 채용되리라 기대된다. In this paper, a in situ hybridization(ISH) has been used to investigate the yield of viral RNA expression from each organ tissues. It is studied to establish a rapidly, specific diagnostic method detecting rabbit haemorrhagic disease virus(RHDV) RNA in 10% formalin-fixed, paraffin-embedded tissues of naturally RHDV-infected rabbits using oligonucleotide probe to be made by RHDV total sequences. Biotin was used as the oligonucleotide probe marker. In situ hybridization is detected the virus genome in the cells and tissue as specifically compared with others nucleic acid hybridization method. All ISH procedure of RHDV were completed to MicroProbe™ capillary action system within 1-2 hours. In this report, RHDV was distributed widely in the cytoplasm of liver cell and the cortex of kidney but lung tissue and medulla of kidney were showed to positive reaction at locally. Although not entirely free of technical limitations, nucleic acid identification holds advantages over other diagnostic tests, including exquisite sensitivity, specificity, interchangeability and speed. It is expected that, in the immediate future viral nucleic acid detection will be a prominent part of the methods used in histopathology.

사회구조적 기능성 왜곡으로 인한 피해자로서의 법비행 청소년 연구

최원기(Choi, Wonki),정윤정(Chung, Yunchung) 한국피해자학회 2003 被害者學硏究 Vol.11 No.1

Generally, the distinction between assailant and victim is constructed by the direction of the behavior resulted. However, when we consider the juvenile delinquence in the aspect of durkheimmien concept, "social fact", we can confirm that this distinction can be newly defined not only at the individual level but also at the social structure level. That is, we can say that, in this aspect, the juvenile assailant can be another victim resulted from the wrong operation of the social structure including him. In this proposition, this study offer us an analysis on the socio-structural cause through a multi-dimensional method with the official statistic data, the question research and interview research in the inductive perspective.

[용어와 건축] 창(Window): 건물 에너지 절감의 시작

최원기(Choi, Won-Ki) 대한건축학회 2022 建築 Vol.66 No.3

설계지표로써 창바닥비의 타당성 연구

최원기(Choi Won-Ki),이현수(Lee Hyun-Soo),엄재용(Eom, Jae-Yong),이충국(Lee Chung-Kook) 한국태양에너지학회 2016 한국태양에너지학회 학술대회논문집 Vol.2016 No.3

건물의 열환경 조절을 위한 태양열 교환 시스템의 응용

최원기(Won-Ki Choi),최창호(Chang-Ho Choi),서승직(Seung-Jik Suh) 대한설비공학회 2000 대한설비공학회 학술발표대회논문집 Vol.2000 No.-