배리어프리(Barrier-Free) 영화 활성화 방안

최영환(Choi, Youngwhan),신주연(Shin, Juyeon),염은유(Yeom, Eunryu),오강국(Oh, Ganggook),오세은(Oh, Seeun),윤혜정(Youn, Hyejeong),정현지(Jeong, Hyunjee),최윤경(Choi, Younkyoung),한지나(Han, Jina),홍소망(Hong, Somang),조성희(Cho, Sunghu 한국상담복지경영학회 2019 상담복지경영연구 Vol.- No.3

본 연구는 장애인의 삶의 질과 밀접한 관련성을 가진 문화향유권을 증진하기 위한 방안을 살펴보기 위한 목적으로 수행되었다. 특히 다양한 문화활동 중 장애인들이 가장 많이 이용하고 있는 활동인 영화 관람에 초점을 맞추었다. 이를 위하여 국내외 배리어프리 영화의 실태와 제도를 살펴보았다. 또한 배리어프리 영화 상영 경험에 따른 배리어프리 영화에 대한 수용도와 장애인에 대한 인식 변화를 검증하였다. 추가적으로 관련 전문가들을 대상으로 인터뷰를 통해 배리어프리 영화의 실태와 활성화 방안을 모색하였다. 연구결과를 살펴보면, 국내의 배리어프리 영화는 2010년대 이후 발전해 오고 있으나, 여전히 장애인들의 문화 활동에 대한 욕구를 충족시키기에는 미흡한 수준이다. 특히 배리어프리 영화의 절대적인 양적 측면의 성장이 미흡한 상황이므로 이에 대한 대책이 필요하다. 국내 배리어프리 영화의 확대를 위한 방안 모색에 있어서 영국은 좋은 사례가 될 수 있으므로 이에 대한 추가적인 검토가 필요하다. 배리어프리 영화 경험에 따른 배리어프리 영화에 대한 수용도와 장애인에 대한 인식 변화를 살펴본 결과에서는 배리어프리 영화를 경험한 경우 배리어프리 영화에 대한 수용도가 증가하고, 장애인에 대한 인식 역시 개선되는 것으로 나타났다. 이러한 결과를 기반으로 하여 배리어프리 영화 경험 증대를 위한 기회 확대, 기업차원이 활성화 방안 추진, 제도의 개선을 제시하였다. The purpose of this study was to find measures to promote cultural rights that are closely related to the quality of life of people with disabilities. In particular, the focus was on movies, which are the most used films for people with disabilities in various cultural activities. We examined the actual conditions and systems of barrier-free films at home and abroad. Also, according to the barrier-free movie screening experience, we examined the change of acceptance of barrier-free movie and perception of disabled people. Additionally, interviews were conducted with relevant experts to find out the status of barrier-free films and how to activate them. Result shows that barrier-free films in Korea have evolved since the 2010s, but are still insufficient to meet the needs of disabled people for their cultural activities. In particular, since the absolute quantitative growth of barrier-free movies is not enough, measures need to be taken. The UK can be a good example of exploring ways to expand domestic barrier-free films, and additional consideration is needed. As a result of examining barrier-free film acceptance and recognition of disabled people according to barrier-free film experience, the barrier-free film experience showed that acceptance of barrier-free film increased and recognition of disabled people also improved. On the basis of results, this study suggested the expansion of opportunities to increase the barrier-free movie experience, the promotion of corporate-level activation, and the improvement of the system.

토마토(Solanum lycopersicum L.) 품종 간 수용성 비타민과 폴리페놀계 성분 함량 변이 분석

김다은,손병구,최영환,강점순,이용재,제병일,박영훈,Kim, Daen,Son, Beunggu,Choi, Youngwhan,Kang, Jumsoon,Lee, Yongjae,Je, Beungil,Park, Younghoon 한국식물생명공학회 2020 식물생명공학회지 Vol.47 No.1

기능성 성분이 향상된 토마토 품종 개발을 위해서는 성분 정량분석법과 토마토 유전자원 간 대사성분 변이에 대한 정보의 확보가 필요하다. 본 연구에서는 토마토 유전자원23개 계통과 12개 상용 F₁ 품종을 이용하여 수용성 비타민 7종(vitamin C, B1, B2, B3, B5, B6, B9)과 폴리페놀계 성분 5종(quercetin, rutin, kaempferol, myricetin, and naringenin chalcone)에 대한 함량을 비교 분석 하였다. HPLC와 LC-MS 분석 결과, 수용성 비타민과 폴리페놀계의 주요 성분으로 vitamin C와 naringenin chalcone이 각각 검출되었으며 품종 간 높은 수준의 함량 변이가 존재함을 알 수 있었다. 반면에 vitamin B1, quercetin 과 kaempferol은 전 품종에 있어 함량이 가장 낮았다. 대사성분 함량과 토마토 과실특성 간 상관관계에 있어 서 과크기(과중)와 높은 유의성이 관찰되었는데 대부분의 성분에 있어 방울토마토 품종이 완숙용 토마토 품종보다 높은 함량을 보였다. 하지만 naringenin chalcone을 제외하고 대사성분과 과색 간의 상관관계는 뚜렷하게 나타나지 않았다. 본 결과는 토마토 육종과정에 활용될 수 있는 효율적인 대사성분 정량분석법을 제시할 뿐만 아니라 기능성 성분 고함량 육종소재 선발에 중요한 정보를 제공한다. Tomato fruit quality is determined by the contents of various functional metabolites in addition to fruit appearance. To develop tomato cultivars with higher amounts of functional compounds, an efficient quantification method is required to identify the natural variations in the compounds in the tomato germplasm. In this study, we investigated tomato varieties, which included 23 inbred lines and 12 commercial F₁ cultivars, for their contents of seven watersoluble vitamins (vitamin C, vitamins B1, B2, B3, B5, B6, and B9) and five polyphenolic compounds (quercetin, rutin, kaempferol, myricetin, and naringenin chalcone). The results of high performance liquid chromatography and liquid chromatography-mass spectrometry showed that vitamin C and naringenin chalcone were the major water-soluble vitamins and polyphenolic compounds, respectively, and their abundance was highly variable depending on the cultivar. By contrast, the contents of vitamin B1, quercetin, and kaempferol were lowest among the cultivars. With regard to the relationship between metabolic compounds and fruit characteristics, a significant association was found in fruit size, indicating that cherry tomato varieties contain higher amounts of the compounds compared to large fresh-type varieties. However, no direct association was detected in fruit color, except for naringenin chalcone. The results of this study provide new insights on the quantification of metabolic compounds and the selection of breeding materials, which are prerequisites for the development of functional tomato varieties.

수박 엘리트 계통의 GBS를 통한 마커이용 육종용 SNP 마커 개발

이준우,손병구,최영환,강점순,이용재,제병일,박영훈,Lee, Junewoo,Son, Beunggu,Choi, Youngwhan,Kang, Jumsoon,Lee, Youngjae,Je, Byoung Il,Park, Younghoon 한국식물생명공학회 2018 식물생명공학회지 Vol.45 No.3

본 연구는 국내 육종 회사에서 개발된 수박(Citrullus lanatus L.) 우량 육성계통 20종을 대상으로 Genotyping-by-sequencing(GBS) 분석을 통해 품종식별, 순도검정, 그리고 마커이용여교잡(Marker-assisted backcross, MABC)용 SNP 세트를 개발하고자 수행되었다. GBS 분석 결과 총 1,100,000천개 raw read 중 77%가 수박 유전체에 mapping되었으며 평균 mapping region은 약 4,000 Kb로 2.3%의 genome coverage를 보였다. Filtering을 통해 평균 depth 31.57의 SNP 총 2,670개를 얻었으며, 20개 계통에 대한 이들의 Polymorphic information content(PIC) 값의 범위는 0.1 ~ 0.38 였다. 이 중 PIC 값이0.3이상이며 각 염색체 별로 5개씩 균등히 분포된 SNP 총 55개를 최종 선발하였다. 사용된 20개 계통의 유연관계분석을 위해 선발된 55개 SNP를 기반으로 한 주성분 분석(Principle component analysis, PCA) 결과 주성분 1 (52%)과 주성분 2 (11%)를 기준으로 4개의 그룹으로 분류 되었으며 각 계통 간 유전자형에 따른 뚜렷한 식별이 가능하였다. 계층적 군집화(Hierarchical clustering) 분석에서도PCA에서와 유사한 분류양상을 관찰할 수 있었다. 따라서 본 연구에서 개발된 SNP 세트는 적용 가능성이 검증된 20개 계통뿐 만 아니라 향후 다양한 수박 육종소재 및 품종에 대한 품종식별, F1 순도검정 및 MABC에 활용될 수 있으리라 기대된다. This study was conducted to develop an SNP set that can be useful for marker-assisted breeding (MAB) in watermelon (Citrullus. lanatus L) using Genotyping-by-sequencing (GBS) analysis of 20 commercial elite watermelon inbreds. The result of GBS showed that 77% of approximately 1.1 billion raw reads were mapped on the watermelon genome with an average mapping region of about 4,000 Kb, which indicated genome coverage of 2.3%. After the filtering process, a total of 2,670 SNPs with an average depth of 31.57 and the PIC (Polymorphic Information Content) value of 0.1~0.38 for 20 elite inbreds were obtained. Among those SNPs, 55 SNPs (5 SNPs per chromosome that are equally distributed on each chromosome) were selected. For the understanding genetic relationship of 20 elite inbreds, PCA (Principal Component Analysis) was carried out with 55 SNPs, which resulted in the classification of inbreds into 4 groups based on PC1 (52%) and PC2 (11%), thus causing differentiation between the inbreds. A similar classification pattern for PCA was observed from hierarchical clustering analysis. The SNP set developed in this study has the potential for application to cultivar identification, F1 seed purity test, and marker-assisted backcross (MABC) not only for 20 elite inbreds but also for diverse resources for watermelon breeding.

GT/FC 혼합발전용 구동연소기 개발

김형모(Hyungmo Kim),박부민(Poomin Park),최영환(Youngwhan Choi),양수석(Sooseok Yang),이대성(Daesung Lee) 대한기계학회 2006 대한기계학회 춘추학술대회 Vol.2006 No.6

??Currently, 60㎾ class gas turbine - fuel cell hybrid power generation system is being developed by Korea Aerospace Research Institute. Because of connection problems between gas turbine and fuel cell, the combustor is necessarily to have flame stability and wide flamability range at the starting stages. Furthermore, in order to enhance the efficiency of the whole system, high combustion efficiency and low pressure loss are required. It also should satisfy the temperature limitations of turbine blade by supplying sufficiently low and uniform thermal energy. In the present study, for the optimization of the combustor, its stability characteristics are tested with change of various operating conditions such as injector shape, swirler air mass flow rate etc.

조 · 만생종 완전단감의 과실발달 단계별 형질특성 및 유전자 발현 분석

김은경(Eun-Gyeong Kim),안광환(Gwang-Hwan Ahn),박기림(Girim Park),손병구(Beunggu Son),최영환(Youngwhan Choi),강점순(Jumsoon Kang),이용재(Youngjae Lee),제병일(Byeong Il Je),박영훈(Younghoon Park) 한국원예학회 2021 원예과학기술지 Vol.39 No.3

We analyzed the major fruit traits and transcriptomes between a late-ripening cultivar ‘Fuyu’ and an early-ripening cultivar ‘Soshu’ of pollination constant non-astringent persimmon (Diospyros kaki Thunb.) at different developmental stages. Sugar concentration and fruit peel color were measured every 3 weeks, until 18 weeks after full bloom (WAB) for ‘Soshu’ and 24 WAB for ‘Fuyu’. For the sugar concentration in fruit, sucrose levels began to decline 9 weeks earlier in ‘Soshu’. Fruit peel coloring progressed faster in ‘Soshu’, and differences in color between the cultivars were most notable at 18 WAB. Transcriptome analysis was carried out for fruit flesh every 3 weeks from 9-18 WAB. Gene ontology term analysis of the transcriptome indicated that catalytic and transport genes involved in metabolism and growth in cells and cellular organelles were mainly expressed, while in KOG and KEGG analysis, the majority of expressed genes were related to signal transduction, nuclear structure, and starch and sucrose metabolism. Differentially expressed genes were analyzed from two sets of samples: set I, in which fruits sampled at 12, 15, and 18 WAB were compared as the treatment group to the sample collected at 9 WAB as the control for each cultivar; and set II, in which fruit samples of ‘Soshu’ were compared as the treatment group to ‘Fuyu’ as the control at each WAB. For the DEGs of set I, containing three comparison combinations, the number of up-regulated genes in the treatment sample was highest at 18 WAB in ‘Fuyu’ and at 15 WAB in ‘Soshu’. For the DEGs of set II, the number of down-regulated genes and the number of up-regulated genes were higher in ‘Soshu’ compared to ‘Fuyu’ at 12 WAB and 15 WAB, respectively. We then compared the expression patterns of fruit trait-related genes with the phenotypic changes in traits. Our results indicated that UGPase-1 and AGPase-1, involved in carbohydrate metabolism, and CRTISO and PDS, involved in carotenoid biosynthesis, may induce early fruit ripening in ‘Soshu’. In addition, the transcription factors (TFs) ERF3, which binds to the promoter of ethylene-responsive genes; the MADS-box TFs RIN, AGL, and FUL; and the NAC TF controlling climacteric-type fruit ripening were considered to play a key role in controlling fruit ripening in ‘Fuyu’ and ‘Soshu’.