http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
심재석(Jae-Suk Shim),천경성(Kyeong-Seong Cheon),오주열(Ju-Youl Oh),황해준(Hae-Jun Hwang),윤혜숙(Hye-Suk Yoon),손길만(Gil-Man Shon),김주현(Zhoo-Hyeon Kim) 한국원예학회 2007 원예과학기술지 Vol.25 No.4
국내 딸기 품종 육성에 이용될 수 있는 기초적인 유전 정보를 얻고자 하여 국내 주요 딸기 재배품종 중에서 선정된 8품종간의 diallel cross F₁ 조합을 만들고 이들로부터 당도의 유전 양식을 조사하였다. F₁실생전체평균은 친평균보다 낮고, 친을 능가하는 F₁조합이 거의 없었다. 그러나 상위 10% F₁실생평균은 친을 크게 능가하였으며, 거의 대부분의 조합들이 해당 친보다 높은 반응을 보여주었다. 또한 친의 자식세대(S₁)의 상위 10%실생평균도 해당 친보다 대부분 높은 값을 보여주어 당도면에서의 개선은 용이하고, 자식에 의해서도 친을 능가하는 개체의 선발이 가능한 것으로 나타났다. 조합별 전체 F₁실생평균으로 분석할 때 (Vr, Wr)회귀분석결과는 부분우성, 당도가 낮은 쪽이 우성으로 나타나지만, 조합별 실생 F₁들중 상위 10%의 실생개체들의 평균값을 가지고 분석한다면, (Vr, Wr) 회귀분석에서 당도는 초우성으로 나타나고, 당도가 높은 우수 친이 우성유전자를 가지므로 우수 친의 후대에서 우수 F₁ 실생개체의 발생빈도가 높은 것으로 나타났다. 이러한 결과는 조합능력의 분석에서도 거의 일치하였다. 조합 능력은 GCA가 SCA보다 높았고 둘 다 모두 유의 하였다. 해당 조합의 F₁실생평균치나 SCA가 높다고 해서 상위10% 실생의 평균치나 상위 10% 실생의 SCA가 높은 것은 아니었다. 반면 상위 10실생평균치가 높을수록 상위 10%SCA는 높았다. 우수한 후대를 많이 생산할 가능성이 높은 친은 실험에 사용된 8개 품종들 중에서 ‘도치오도메’, ‘매향’ ‘아끼히메’, ‘여봉’, 등이었으며, 이중 특히 ‘매향’과 ‘도치오도메’가 좋은 친일 것으로 고려되었다. 가장 우수한 조합은 ‘매향’× ‘조홍’, ‘매향’× ‘도치오도메’, ‘매향’× ‘도요노카’ 등이며 다음으로 ‘매향’× ‘여봉’ ‘매향’× ‘아끼히메’ ‘조홍’× ‘도치오도메’ ‘도치오도메’× ‘여봉’ ‘도치오도메’× ‘레드펄’ 등이었다. Forty one germplasm accessions were tested for their soluble solid contents (SSC). Of them, eight accessions which are the leading cultivars, showing significant difference among them, were selected and crossed each other for making 8-parents half-diallel set. The expression of SSC of the F1s varied depending upon the parents. Average SSC of all F₁ seedlings was lower than the mean of all parents, but similar to or a little higher than the mean of all S₁ seedlings obtained from selfed parents. The mean of all S₁ seedlings were lower than those of all parents. The mean of superior 10% of F₁ seedlings of each cross was almost higher than superior parents and the mean of superior 10% S₁ seedlings. Also, almost superior 10% S₁ exceeded over their parents. In (Vr, Wr) regression analysis, when using parents and means of all F₁ seedlings of each cross, the direction of dominance was toward to lower SSC, but when using parents and means of superior 10% F₁ seedlings of each cross, higher SSC was expressed in over-dominance mode, which could expect much more superior F₁ seedlings in the combinations with higher SSC parents. The results in analysis of combining ability, which both GCA and SCA were highly significant, were similar with those in regression analysis, and variance of GCA was larger than that of SCA. The higher in the mean SSC of all F₁ seedlings did not always guarantee the higher mean SSC of superior 10% F₁ seedlings. While the higher mean SSC of superior 10% F₁ seedlings was, the larger SCA of superior 10% F₁ seedlings was observed. The better parents, expected for producing more superior F₁ seedlings, were ‘Maehyang’, ‘Tochiotome’, ‘Akihime’, and ‘Yeobong’. Of them, ‘Maehyang’ and ‘Tochiodome’ were the best parents in the respect on improving SSC. The best combinations for increasing SSC were ‘Maehyang’ x ‘Johong’, ‘Maehyang’ x ‘Tochiotome’, ‘Maehyang’ x ‘Toyonoka’, followed by ‘Maehyang’ x ‘Yeobong’, ‘Maehyang’ x ‘Akihime’, ‘Johong’ x ‘Tochiotome’, ‘Tochiotome’ x ‘Yeobong’, and ‘Tochiotome’ x ‘Red pearl’, in order.
MutMap 분석에 의한 벼 왜성 돌연변이 계통의 변이 유전자 탐색
오준(Jun Oh),천경성(Kyeong-Seong Cheon),강도유(Do-Yu Kang),김송림(Song Lim Kim),이은경(Eungyeong Lee),김년희(Nyunhee Kim),오효자(Hyoja Oh),최인찬(Inchan Choi),백정호(Jeongho Baek),윤인선(In Sun Yoon),김경환(Kyung-Hwan Kim),정남진(Nam-J 한국육종학회 2020 한국육종학회지 Vol.52 No.1
A dwarf mutant rice line was selected from an Ac/Ds insertion mutant population and named dwf1. The phenotype of F1 and F2 plants derived from a cross between dwf1 and Dongjin indicated that a single recessive gene is responsible for the mutant phenotype, and we named this gene dwf1. Resequencing of the dwf1 line and Dongjin (wild type) revealed 42,386 homozygous single nucleotide polymorphisms (SNPs) between dwf1 line and Dongjin. MutMap analysis was performed by sequencing a DNA pool prepared from 100 mutant type plants in the dwf1/Dongjin F2 population, and it was found that the dwf1 gene was located in the 23 ~ 30 Mbp region on chromosome 4. In this region, we found a non-synonymous SNP in the Os04g0469800 gene, which was reported as D11 gene encoding a cytochrome P450 family protein involved in the biosynthesis of brassinosteroids (BRs). This SNP was regarded as the causative SNP for the dwf1 phenotype, and the dwf1 gene is a novel allele of D11. We performed mapping of the dwf1 gene with five SNP markers on chromosome 4 with 190 dwf1/Dongjin F2 plants. The phenotype of F2 plants was completely co-segregated with genotypes of the J10402 marker, which was developed based on the non-synonymous SNP in the D11 gene. These results will contribute to the study of the molecular biological functions of the D11 gene and BRs.