PNMT유전자 조각의 shot-gun cloning에 관한 연구

조홍범,최영길 한국미생물학회 1990 微生物과 産業 Vol.16 No.1

동물체의 신경전달물질은 acetylcholine을 비롯한 9종 이상의 물질이 알려져 있으며 이들 물질의 생체내에서의 기작과 생합성 과정에 관해서는 많이 알려져 있고 또한 이 방면의 연구도 활발하다. 그중에도 Joh와 Baetge등에 의하여 DBH와 PNMT 효소의 생산에 관여하는 mRNA가 면역침전법에 의하여 순수 분리되고 이를 주형으로 하여 DBH와 PNMT 효소 생산에 근본이 되는 유전자의 염기서열 및 유전자의 구조를 규명하는 작업이 진행되고 있으나 아직도 그 전체가 규명된 바는 없다 (Baetge등, 1981;1983;Joh등, 1983;1984). 그리하여 본인들은 상기의 연구자들로부터 PNMT 유전자인 cDNA를 M13mp18과 19의 vector phage에 재조합시키고 이 cDNA를 JM107rhk 109의 host bacteria에 도입하여 형질발현 실험을 통하여 확인하고자 하였다.

롯데 인재개발원 오산캠퍼스 리빌딩

조홍범,주현태,박경진,최재원,김영일 한국건축시공학회 2021 건축시공 Vol.21 No.4

토양세균 군집의 16S rDNA 제한효소 지문 분석

조홍범 서경대학교 산업기술연구소 1998 産業技術硏究所論文集 Vol.5 No.-

To investigate the differences of soil bacterial diversity according to 5vegetation types, restriction enzyme patterns of 16S rDNA were analyzed. From the fingerprints by amplified rDNA restriction analysis(ARDRA), soil samples were grouped three kinds of soil microbial communities as forest, grass-agricultured and naked soil. Genetic similarity was lowest between direct extracted soil DNA and indirect extracted from heterotrophic bacteria that cultured in LB medium.

Comparison between L and E gene amplification analytical methods for human papillomavirus typing

조홍범,김경태,김영재 대한부인종양학회 2008 Journal of Gynecologic Oncology Vol.19 No.4

Objective: L and E6/E7 gene amplification analyses were compared to identify human papillomavirus (HPV) infection and verify the HPV type, with the intent to minimize HPV typing errors. Methods: L1 gene verified HPV typing was accomplished via polymerase chain reaction (PCR) and membrane assays. Verification of HPV typing via E6/E7 genes was accomplished through nested multiplexed PCR. The results from 104 samples were compared. Results: The rates of accordance and difference were 35% and 65%, respectively. For 29% of the analyses, nested multiplexed PCR was more diversified than the membrane assay. Conclusion: HPV can be classified into low-risk HPV and high-risk HPV groups. In parallel amplifications of the L and E genes is more efficient for accurate diagnosis in light of the different symptoms and attendant precautions of the risk groups. Objective: L and E6/E7 gene amplification analyses were compared to identify human papillomavirus (HPV) infection and verify the HPV type, with the intent to minimize HPV typing errors. Methods: L1 gene verified HPV typing was accomplished via polymerase chain reaction (PCR) and membrane assays. Verification of HPV typing via E6/E7 genes was accomplished through nested multiplexed PCR. The results from 104 samples were compared. Results: The rates of accordance and difference were 35% and 65%, respectively. For 29% of the analyses, nested multiplexed PCR was more diversified than the membrane assay. Conclusion: HPV can be classified into low-risk HPV and high-risk HPV groups. In parallel amplifications of the L and E genes is more efficient for accurate diagnosis in light of the different symptoms and attendant precautions of the risk groups.

Cementing Efficiency of Fly-ash in Mortar Matrix According to Binder-Water Ratio and Fly-ash Replacement Ratio

조홍범,지남용 한국건축시공학회 2012 한국건축시공학회지 Vol.12 No.2

This paper predicts the cementing efficiency of fly-ash(FA) based on mortar test considering binder-water ratio and FA replacement ratio as experimental variables. The cementing efficiency prediction model proposed by statistical analysis enables us to estimate the value according to the binder-water ratio and FA replacement ratio of matrix. When FA replacement ratio is the same, the lower the binder-water ratio, the higher the estimated cementing efficiency. There are significant differences in the values according to binder-water ratio at FA replacement ratios of 15% or less, but there are almost no differences when FA replacement ratio is more than 15%. As the binder-water ratio increases, the variations in the values according to FA replacement ratio are great at FA replacement ratios of 15% or less. As the FA replacement ratios increase, the values increase for FA replacement ratios of 15% or less, but decrease for more than 15%. The values range from -0.71 to 1.24 at binder-water ratio of 1.67-2.86 and FA replacement ratio of 0-70%. The RMSE of the 28-day compressive strength predicted by modified water-cement ratio is 2.2 MPa. The values can be trusted, as there is good agreement between predicted strength and experimental strength.

Multiplex Real-Time PCR을 이용하여 6종의 주요 잇몸질환 유발 미생물을 동시에 검출하는 기법

조홍범,Cho, Hong-Bum 한국미생물학회 2013 미생물학회지 Vol.49 No.3

This study utilized an analysis method for detecting six microorganisms, such as Actinobacillus actinomycetemcomitans, Campylobacter rectus, Porphyromonas gingivalis, Tannerella forsythus, Treponema denticola, and Prevotella intermedia, triggering periodontal disease, using multiplex real-time polymerase chain reaction (PCR). The analysis including internal control was made by dividing the six species into two groups using four fluorescence dyes, and it was verified that there was no interference or cross-reaction between the target species and different kinds of oral microbial species. Qualitative and quantitative analyses were conducted on each microorganism in various samples, such as saliva and the plaque, using the multiplex real-time PCR and comparative analysis between periodontitis patients and healthy people, revealing obvious differences between them. 본 연구는 multiplex real-time PCR을 이용하여 Actinobacillus actinomycetemcomitans, Campylobacter rectus, Porphyromonas gingivalis, Tannerella forsythus, Treponema denticola, Prevotella intermedia 등과 같은 6종의 주요 치주 질환 원인 미생물들을 동시에 검출할 수 있는 분석 방법에 관한 것이다. 4개의 형광 염료를 사용하여 internal control과 함께 3개의 균종씩 나누어 분석하였으며, 분석 대상 균종 간 그리고 다른 종류의 구강 미생물 균종과의 간섭과 교차 반응이 없음을 확인하였다. 본 연구의 multiplex real-time PCR은 타액과 플라그 등의 다양한 샘플에 포함되어 있는 각 미생물들을 정성, 정량적으로 분석할 수 있었으며, 치주염 환자와 건강한 사람들에 대한 비교 분석 결과 분명한 차이를 발견 할 수 있었다.

Guanidine isothiocyanate와 silica bead를 이용한 토양내 미생물의 핵산 추출 방법

조홍범 서경대학교 산업기술연구소 2009 産業技術硏究所論文集 Vol.23 No.-

In this study, we applied polymerase chain reaction method to detect Pseudomonas aeruginosa from soil and used guanidine isothiocyanate and silica bead to extract genomic DNA. The algD gene of P. aeruginosa was selected for specific detection and primer sets were designed (forward [5-ggccatcaagttggtatcaagt-3], reverse [5-atgctgattcgcatcgcat-3]). Existing DNA extraction methods based on guanidine & silica bead were compared with other old methods. The sensitivity of the guanidine-based method was significantly higher than that of the others. The better sensitivity of guanidine & silica based DNA extraction could be explained by the finding that the method using guanidine isothiocyanate & silica has a greater ability than the other methods for elimination of PCR reaction inhibitor.

Betaine을 이용한 non-specific PCR 반응의 억제 효과

조홍범 서경대학교 산업기술연구소 2007 産業技術硏究所論文集 Vol.18 No.-

Betaine has been applied to improve PCR amplification of GC-rich DNA sequences. Recently, betaine is not only used for improving binding efficiency of spotted PCR products and reducing non-specific background signal in DNA microarrays, but enhancing efficiency of PCR reaction. In this study, betaine as the PCR additive was used for restraining non-specific amplification. Non-specific PCR product was not obtained in this study and PCR enhancing effect was not observed.

Multiplex PCR을 이용한 DNA size marker 제조 기법

조홍범 서경대학교 산업기술연구소 2007 産業技術硏究所論文集 Vol.18 No.-

DNA size marker is a very routinely used reagent in DNA analysis work, but it`s high price has prevented its usage. Then, in this study, we have developed in-house manufacturing method of DNA size marker using multiplex PCR techniques. 100 base pair ladder were produced and compared with commercially selling DNA size markers.

종속영양세균 군집의 종 조성 변화와 G+C 함량의 상관관계에 미치는 산성화의 영향

조홍범 서경대학교 산업기술연구소 1997 産業技術硏究所論文集 Vol.2 No.-

The G+C contents of 202 heterotrophic bacterial strains isolated from pH gracuent microcosm were compared with the various pH range(7, 6, 5, 4 and3) to their distribution, The G+C contents of heteroptrophic bacterial isolates were ranged from 22.8 to 78 mol%. As pH decreased, the average amounts of G+C were increased while the change of G+C contents in each pH level was decreased. In the case of interspecies of some genuses such as Procidencia and .Pseudorunas, the average amount of G+C increased as pH became lower. It is find that even the same genus can have a different G+C content depending on acidic stress, and G+C content of bacteria increases incrementally with of environmental stress.