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류마티스 관절염 환자에서 Conserved T 세포 수용체의 CDR3 motif를 표현하는 제2형 콜라겐 특이 T세포주의 형성과 유지
김승훈,조미라,윤지희,박성환,조철수,황수연,김호연,Kim, Seung-Hoon,Cho, Mi-La,Youn, Jeehee,Park, Sung-Hwan,Hwang, Sue-Yun,Cho, Chul-Soo,Kim, Ho-Youn 대한면역학회 2001 Immune Network Vol.1 No.1
Background: To determine the molecular structure of type II collagen-specific T-cell receptors associated with rheumatoid arthritis (RA). Methods: We generated CII-specific T-cell lines of 8 RA patients by prolonged in vitro culture with bovine CII (bCII) and the immunogenic peptide (256-270) of human CII. The proliferation response towards CII stimulation was measured from the uptake of 3H-thymidine. Changes in the secretion of Th 1 and Th2 cytokines in the culture supernatent were measured by ELISA. The TCR clonotypes of these T-cells were examined by RT-PCR/SSCP analyses of all 22 $V_{\beta}$ chains. Results: T-cells from patients' tissue exhibited strong proliferation index upon CII stimulation, which was maintained up to 6 months in the culture. The secretion of INF-$\gamma$from these T-cells increased along with the duration of culture time, while the amount of IL-4 production did not show significant changes. The SSCP band patterns of patients' T-cells appear as discrete bands unlike the smeary streak produced from normal samples. Some SSCP bands, each representing selected expansion of a TCR containing certain subtype of $V_{\beta}$ peptides, appeared to be identical in more than one patients. Among these, the expansion of SSCP band representing the $V_{\beta}$ 14 CDR3 region persisted after switching the antigen to the immunogenic human peptide (256-270). Conclusion: CII-reactive T-cells expressing distinct CDR3 motifs are selectively expanded in the peripheral blood and synovial fluid of RA patients, and their persistent proliferation upon CII stimulation, as well as the production Th 1-type cytokines, may play pivotal roles in RA pathogenesis.
흰쥐의 제 2 형 Collagen 유발 관절염 : IgG 항 Collagen 항체의 변화
박동준(Dong Jun Park),조철수(Chul Soo Cho),김호연(Ho Youn Kim),김동집(Dong Jip Kim) 대한내과학회 1991 대한내과학회지 Vol.41 No.3
N/A Collagen-induced arrthritis (CIA) is a chronic inflammatory arthritis in rodents immunized with type II collagen, a major protein of hyaline cartilage. Because CIA shares clinical, histologic, and immunologic features with human rheumatoid arthritis, it has been regarded as an important animal model for elucidation of the pathogenesis and therapeutic approach to this disease. To investigate the role of humoral immunity to type II collagen in the initiation of CIA, the authors evaluate the clinical features and sequential changes of IgG anticollagen antibody(ACA) in CIA(n=6), IgG ACA to native bocine type II collagen was measured by enzyme-linked immunosorbent assay. The results were as follows: 1) The incidence of CIA was 67% (6 out of 9 rats), and the mean number of involved limbs per rat in the arthritic group was 2.2±0.4. The mean day of arthritic onset was the 22th day, ranging from the 12th to the 25th day after immunization. 2) IgG ACA to native bovine type II collagen in the arthritic group (n=6) was significantly higher than that in the control group (n=9) from the 2nd to the 8th week after initial immunization (p<0.003). In the arthritic group IgG ACA reached a peak level in the 4th week and then slightly decreased. However, it showed a persistently higher level until the 8th week after immunization, Th results suggest that humoral immunity to type II collagen has an important role in the initiation of CIA.
한국인의 각종 질환에서 anti - HCV 의 검출 양상
박영민(Young Min Park),조철수(Chul Soo Cho),한남익(Nam Ick Hahn),김인식(In Sik Kim),김용수(Young Soo Kim),임계순(Gae Soon Lim),정진우(Jin Woo Chung),윤영석(Young Suk Yoon),이창돈(Chang Don Lee),김호연(Ho Yeon Kim),방병기(Byung Kee Ba 대한내과학회 1991 대한내과학회지 Vol.41 No.2
N/A We studied the prevalence of anti-HCV in 585 sera from various individuals using enzyme immunoassay (EIA, Abbott Laboraties, USA). The results were as follows: 1) Anti-HCV was detected in 16 (10.7%) out of 150 HBsAg positive liver diseases diagnosed by liver biopsy, consisting of none out of 10 acute viral hepatitis, 3 out of 15 chronic persistent hepatitis, 4 out of 50 chronic active hepatitis, 2 out of 32 liver cirrhosis, and 7 out of 43 hepatocellular carcinoma. 2) Anti-HCV was detected in 43 (45.3%) out of 95 HBsAg negative liver diseases diagnosed by liver biopsy, consisting of 5 out of 8 acute viral hepatitis, 2 out of 10 chronic persistent hepatitis, 17 out of 30 chronic active hepatitis, 4 out of 15 liver cirrhosis, and 15 out of 32 hepatocellular carcinoma. 3) Anti-HCV was detected in 22 (38.6%) out of 57 hemodialysis patients, in 3 (6.7%) out of 45 Kidney transplants, in 2 (11.1%) out of 18 fatty liver diagnosed by liver biopsy, in 2 (1.3%) out of 150 healthy blood donors, and none out of 40 healthy volunteers, 4) Anti-HCV was detected in 6 (31.6%) out of 19 rheumatoid arthritis and 6 (54.5%) out of 11 systemic lupus erythematosis. 5) There were familial clusters of chronic liver diseases in 4.7% of the patients with HRsAg negative/ anti-HCV positive chronic liver diseases and in 19.4% of the patients with HBsAg positive/anti-HCV negative liver diseases. 6) The incidence of anti-HCV within HBsAg positive liver diseases was higher in HBsAg negative patients (17.6%) than in HBeAg positive patients (10.3%). 7) In the hemodialysis patients, the number of hemodialysis was significantly higher in anti-HCV positive patients than in anti-HCV negative patients (p<0.009), but the number of blood transfusions didn't indicate a difference between anti-HCV positive and negative patients. We concluded that 1) HCV might be an important cause of various types of HBsAg negative liver diseases, 2) intrafamilial transmission of HCV might be much less common than of HBV, 3) coinfections of both HCV and HBV might be relatively common, 4) long duration of hemodialysis might be related to the increment of incidence of anti-HCV in hemodialysis units, and 5) the high frequency of false-positive anti-HCV in autoimmune disorders without evidence of liver diseases might limit the use of the current anti-HCV tests.
Elevated Serum Levels of Both TH1 1 and TH2 Cytokines in Patients with Adult Still`s Disease
민준기 ( Jun Ki Min ),조미라 ( Mi Ra Cho ),민소연 ( So Yeon Min ),김선준 ( Sun June Kim ),김완욱 ( Wan Uk Kim ),홍연식 ( Yeon Sik Hong ),박성환 ( Sung Hwan Park ),조철수 ( Chul Soo Cho ),김호연 ( Ho Youn Kim ) 대한내과학회 1999 대한내과학회 추계학술대회 Vol.57 No.-
이신석 ( Shin Seok Lee ),조철수 ( Chul Soo Cho ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.4
Rheumatoid arthritis (RA) is characterized by synovial cell hyperplasia and the overgrowth of a fibrovascular granulation tissue, known as pannus, which proliferates like tumor cells and destroys cartilage and bone within the joint. Since neovascularization is necessary for the continual proliferation of synovial tissue, it is believed to play an important role in the development and progression of RA. Angiogenesis in the inflamed joints represents the net balance between the effects of angiogenic and anti-angiogenic factors. A number of angiogenic factors have been described in RA, of which vascular endothelial growth factor (VEGF) and basic fibroblast growth factor are the most potent. In RA, high levels of VEGF are present in synovial fluid and synovial tissue, which is produced by cells near endothelial cells and acts on endothelial cells via interaction with its receptors. VEGF is induced by hypoxia, which may occur in the inflamed joint. Not only do cytokines like interleukin (IL)-1β, IL-6, and TGF-β induce fibroblast expression of VEGF, but so does engagement of CD40 ligand. Recently, specific inhibition of VEGF by soluble receptor, sflt-1, and by anti-VEGF monoclonal antibody has been shown to attenuate collagen-induced arthritis in mice, supporting the major role of VEGF in promoting angiogenesis. Efforts are currently targeted at inhibiting angiogenic factors in RA.
Circulating IL - 4 levels in lupus nephritis is increased and downregulated by cyclophosphamide
김성일(Sung Il Kim),조미라(Mi La Cho),이신석(Shin Seok Lee),주영실(Yeong Shil Joo),김완욱(Wan Uk Kim),민준기(Jun Ki Min),홍연식(Yeon Sik Hong),이상헌(Sang Heon Lee),박성환(Sung Hwan Park),조철수(Chul Soo Cho),김호연(Ho Youn Kim) 대한내과학회 1998 대한내과학회 추계학술대회 Vol.55 No.-
항 인지질 항체 양성인 전신성 홍반성 루푸스 환자에서의 무도병
조영석 ( Young Suk Cho ),최범순 ( Bum Soon Choi ),여경아 ( Kyung Ah Yoh ),양형인 ( Hyung In Yang ),박성환 ( Sung Hwan Park ),이상헌 ( Sang Heon Lee ),조철수 ( Chul Soo Cho ),김호연 ( Ho Youn Kim ) 대한류마티스학회 1995 대한류마티스학회지 Vol.2 No.2
The neuropsychiatric manifestations of systemic lupus erythematosus (SLE) are diverse but, among them, movement disorders such as chorea, ataxia are uncommon. We describe a 16 year old female SLE patient with positive anticardiolipin antibody who developed sudden onset of chorea. The clinical and laboratory findings that positive anticardiolipin antibody, thrombocytopenia, chorea were suggested antiphospholipid syndrome. Although the pathophysiology of chorea in SLE is unknown, an association with antiphospholipid antibodies has been suggested. The underlying pathogenic mechanism of this case could not established but was most likely to have been an ischemia of the contralateral subthalamic nucleus. She was treated with corticosteroid, haloperidol and warfarin and now chorea is disappered.
조병식 ( Byoung Sik Cho ),곽승기 ( Seung Ki Kwok ),서영일 ( Young Il Seo ),민도준 ( Do June Min ),김완욱 ( Wan Uk Kim ),이숙경 ( Suk Kyoung Lee ),박성환 ( Sung Hwan Park ),조철수 ( Chul Soo Cho ),김호연 ( Ho Youn Kim ) 대한류마티스학회 2001 대한류마티스학회지 Vol.8 No.4
Rheumatoid arthritis(RA) is occasionally associated with variable extra-articular involvement and the chronic inflammatory process can affect the gastrointestinal system. The gastrointestinal involvement in RA may present in many causes: drug induced colitis, vasculitis and amyloidosis involved in the gut, association with certain bowel diseases such as collagenous colitis or infectious colitis. Ulcerative colitis(UC) is a chronic inflammatory bowel disease and is commonly associated with peripheral joint disease which correlates with the disease activity and extent of the bowel inflammation. The arthritis is usually presented in pauciarticular, generally asymmetric, transient, and nondestructive pattern. However, the chronic and destructive peripheral arthritis has been reported in a few cases and RA has not been observed in association with UC. We experienced a case of RA patient with recurrent abdominal pain, hematochezia, and tenesmus who was diagnosed as UC by endoscopic and histologic finding. We herein report the case with literature.