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Real-Time PCR을 이용한 해수 존재 흰반점 바이러스의 정량 및 양식 환경인자와의 상관관계 분석
송재호,추여진,조장천,Song, Jae-Ho,Choo, Yoe-Jin,Cho, Jang-Cheon 한국미생물학회 2008 미생물학회지 Vol.44 No.1
흰반점 바이러스(white spot syndrome virus, WSSV)는 양식산 새우에 감염하여 대량폐사를 일으키는 전염성이 매우 강한 병원성 바이러스이다. 본 연구에서는 강화도에 위치한 대하(Fenneropenaeus chinensis) 양식장의 양식수와 양식장으로 유입되는 해수에서 WSSV를 막여과법을 이용하여 농축하였으며, 새롭게 디자인한 primer와 Taqman probe를 사용하여 정량 실시간 PCR (quantitative real-time PCR, QRT-PCR)을 적용하여 WSSV를 정량하였다. 농도표준을 사용한 QRT-PCR 결과, 제작된 primer와 probe를 이용하여 WSSV가 정확하고 민감하게 검출됨을 확인하였다. 해수에 존재하는 WSSV와 물리화학적, 생물학적 환경요인간의 상관관계를 도출하기 위하여 양식수와 해수 유입수에서 대하 양식기간인 2007년 6월부터 9월까지 총 8회에 거쳐 다양한 환경요인을 분석하였다. 양식수 1L에 존재하는 WSSV의 양은 3,814-121,545 copy였으며, 이는 분원성 enterococci ($r^2=0.9$, p=0.02), 엽록소${\alpha}$ ($r^2=0.8$, p=0.03), 생화학적 산소요구량($r^2=0.8$, p=0.07)과 상관관계를 나타내었다. 결론적으로 본 연구에서 정립된 WSSV의 농축법 및 QRT-PCR 방법은 해수에 존재하는 WSSV를 정량하는데 효과적이었으며, 해수에 존재하는 WSSV의 양은 물리화학적 환경요인보다 생물학적 환경요인과 밀접한 관련을 보였다. White Spot Syndrome Virus (WSSV) is one of the most virulent viral agents in the penaeid shrimp culture industry. In this study, WSSV in a Fenneropenaeus chinensis shrimp farm and an adjacent seawater were concentrated using a membrane filtration and quantified using the quantitative real-time PCR (QRT-PCR) method with newly designed primers and Taqman probe. Sensitivity of primers and probe was proven by WSSV standard curve assay in QRT-PCR. In order to demonstrate the relationship between WSSV and environmental parameters, physicochemical and biological parameters of the farm and influent seawaters were monitored from June to September, 2007. The abundance of WSSV ranged 3,814-121,546 copies per 1 liter of seawater, which was correlated with fecal enterococci ($r^2=0.9$, p=0.02), chlorophyll ${\alpha}$ ($r^2=0.8$, p=0.03) and $BOD_5$ ($r^2=0.8$, p=0.07). Subsequently, it is concluded that the QRT-PCR method using Taqman probe established in this study was efficient to clarify the quantification of WSSV in seawaters. Statistical analyses of environmental parameters obtained in this study also showed that the abundance of WSSV was correlated with several biological parameters rather than physicochemical parameters.
Cyanobacteria 의 증식에 따른 대청호 생태계내의 생물군집 변화
김명운,김민호,조장천,김상종 ( Myeong Woon Kim,Min Ho Kim,Jang Cheon Cho,Sang Jong Kim ) 한국하천호수학회 1995 생태와 환경 Vol.28 No.1
The changes of primary producer, zooplankton, and characteristics of bacterial community were compared with the bloom of Microcystis aeruginosa in the Chusori, Daechung Reservoir where conventional summer bloom was observed. Primary producer changed from diatoms at spring to M. aeruginosa with the concentration of 4.6x10^8 cells 1^-1 at summer. Zooplankton changed into large-sized copepod. Difference of the total bacterial number between pre-bloom and blooming period was not large. The saprophytic number was average of 1.27×10^4CFUS ml^-1 that was 0.12% of the total bacterial number. Bacterial production rate was 3.95㎍C1^-1hr^-1 before July and 2.77㎍gC 1^-1hr^-1 during blooming period. Characteristics of bacterial community showed bacteria did not readily use increased organic compound.
제주도 숨은물벵뒤 습지 서식 Betaproteobacteria의 종다양성 및 신분류군 분포
신영민 ( Young Min Shin ),김태의 ( Tae Ui Kim ),최아영 ( Ah Young Choi ),전지선 ( Jee Sun Chun ),이상훈 ( Sang Hoon Lee ),김하늘 ( Ha Neul Kim ),이하나 ( Ha Na Yi ),조재형 ( Jae Hyung Jo ),조장천 ( Jang Cheon Cho ),장광엽 ( Kwang 한국환경생물학회 2011 환경생물 : 환경생물학회지 Vol.29 No.3
The species diversity of Betaproteobacteria in the Sumunmulbengdui Wetland Area of Jeju Island was studied using culture based techniques, and candidates for novel taxa were screened. Twenty two novel bacterial strains belonging to Betaproteobacteria were isolated, which could be assigned to 16 genera of 4 families, namely Burkholderiaceae (3 strains), Comamonadaceae (8 strains), Oxalobacteraceae (5 strains), Neisseriaceae (5 strains), and an unassigned group belonging to Burkholderiales (1 strain) based 16S rRNA gene sequences. The genus Chromobacterium contained three candidates of novel species, and each of the genera Burkholderia, Comamonas, Pelomonas and Herbaspirillum contained two candidates respectively. Through the analysis of membrane fatty acid profiles and physiological properties using API 20NE as well as morphological and cultural properties, each of the isolates was found to form potentially novel species. Brief description of 22 potential candidates for new species or subspecies is given accordingly.
제주도 숨은물벵뒤 습지 서식 Alphaproteobacteria 및 Gammaproteobacteria 강에 속하는 신변이주의 특성
김하늘 ( Ha Neul Kim ),강지영 ( Ji Young Kang ),최재희 ( Jae Hee Choi ),최정욱 ( Jeong Uk Choe ),이상훈 ( Sang Hoon Lee ),김태의 ( Tae Ui Kim ),이하나 ( Ha Na Yi ),장광엽 ( Kwang Yeop Jahng ),조장천 ( Jang Cheon Cho ),이현환 ( Hyu 한국환경생물학회 2011 환경생물 : 환경생물학회지 Vol.29 No.3
In this study, samples were collected from the Sumummulbangdui wetland at the Halla Mountain in Jeju Island in order to isolate novel bacterial strain. Bacterial strains belonging to the class Alphaproteobacteria, and Gammaproteobacteria were isolated after spreading samples onto solid agar media. The 16S rRNA gene sequences of the strains assigned to the two classes were compared to those of type strains of the species. The strains that showed less than 98.7% 16S rRNA gene sequence similarity to the validly published species were considered to be novel species candidates. A total of 19 strains were regarded as novel strains which can be regarded as novel species candidates. In the Alphaproteobacteria, 6 novel strains were affiliated with the genera Novosphingobium, and Rhizobium. A total of 13 novel strains belong to Gammaproteobacteria that assigned to the family Moraxellaceae, Pseudomonadaceae, and Enterobacteriaceae were identified. Cultural, physiological, chemotaxonomic characteristics and fatty acids compositions have been determined for the novel species candidates, and the characteristics are described in this study.