활어수송과정에서의 계약체결상의 책임

조은해,박수봉,임석원 한국수산해양기술학회(구 한국어업기술학회) 2024 한국수산해양기술학회 학술발표대회 Vol.2024 No.04

Dual Component Analysis for In Vivo T2* Decay of Hyperpolarized 13C Metabolites

조은해,이준성,이한솔,양승욱,최영숙,왕은경,송호택,김동현 대한자기공명의과학회 2017 Investigative Magnetic Resonance Imaging Vol.21 No.1

Purpose: To investigate the exchange and redistribution of hyperpolarized 13C metabolites between different pools by temporally analyzing the relative fraction of dual T2* components of hyperpolarized 13C metabolites. Materials and Methods: A dual exponential decay analysis of T2* is performed for [1-13C] pyruvate and [1-13C] lactate using nonspatially resolved dynamic 13C MR spectroscopy from mice brains with tumors (n = 3) and without (n = 4) tumors. The values of shorter and longer T2* components are explored when fitted from averaged spectrum and temporal variations of their fractions. Results: The T2* values were not significantly different between the tumor and control groups, but the fraction of longer T2* [1-13C] lactate components was more than 10% in the tumor group over that of the controls (P < 0.1). The fraction of shorter T2* components of [1-13C] pyruvate showed an increasing tendency while that of the [1-13C] lactate was decreasing over time. The slopes of the changing fraction were steeper for the tumor group than the controls, especially for lactate (P < 0.01). In both pyruvate and lactate, the fraction of the shorter T2* component was always greater than the longer T2* component over time. Conclusion: The exchange and redistribution of pyruvate and lactate between different pools was investigated by dual component analysis of the free induction decay signal from hyperpolarized 13C experiments. Tumor and control groups showed differences in their fractions rather than the values of longer and shorter T2* components. Fraction changing dynamics may provide an aspect for extravasation and membrane transport of pyruvate and lactate, and will be useful to determine the appropriate time window for acquisition of hyperpolarized 13C images.

Evaluation of Two Commercial HLA-B27 Real-Time PCR Kits

조은해,이상곤,석정호,박보야나,이은희 대한진단검사의학회 2009 Annals of Laboratory Medicine Vol.29 No.6

Background : The standard PCR with sequence-specific primers (SSP) is a widely used method of HLA-B27 typing in clinical practice. The aim of our study was to evaluate 2 Korean HLA-B27 kits with different real-time PCR chemistries. Methods : To validate the accuracy of real-time PCR kits, we selected 28 HLA-B27-positive samples and 33 HLA-B27-negative samples with a wide range of different HLA-B specificities typed by standard PCR-SSP. The 2 real-time PCR kits used were the AccuPower HLA-B27 real-time PCR kit (Bioneer, Korea) with TaqMan probes and the Real-QTM HLA-B*27 detection kit (BioSewoom, Korea) with SYBR Green I dye for melting curve analysis. Results : All 61 samples typed by PCR-SSP demonstrated a perfect concordance with the 2 realtime PCR assays. It was possible to clearly discriminate between HLA-B27-positive and -negative samples in both real-time assays. Conclusions : In summary, both real-time PCR assays for HLA-B27 were fast, reliable, well-adapted for routine laboratory testing, and attractive alternatives to the conventional PCR-SSP method. Background : The standard PCR with sequence-specific primers (SSP) is a widely used method of HLA-B27 typing in clinical practice. The aim of our study was to evaluate 2 Korean HLA-B27 kits with different real-time PCR chemistries. Methods : To validate the accuracy of real-time PCR kits, we selected 28 HLA-B27-positive samples and 33 HLA-B27-negative samples with a wide range of different HLA-B specificities typed by standard PCR-SSP. The 2 real-time PCR kits used were the AccuPower HLA-B27 real-time PCR kit (Bioneer, Korea) with TaqMan probes and the Real-QTM HLA-B*27 detection kit (BioSewoom, Korea) with SYBR Green I dye for melting curve analysis. Results : All 61 samples typed by PCR-SSP demonstrated a perfect concordance with the 2 realtime PCR assays. It was possible to clearly discriminate between HLA-B27-positive and -negative samples in both real-time assays. Conclusions : In summary, both real-time PCR assays for HLA-B27 were fast, reliable, well-adapted for routine laboratory testing, and attractive alternatives to the conventional PCR-SSP method.

Comparing Two Diagnostic Laboratory Tests for Several Microdeletions Causing Mental Retardation Syndromes: Multiplex Ligation-Dependent Amplification vs Fluorescent In Situ Hybridization

조은해,박보야나,조정희,강유선 대한진단검사의학회 2009 Annals of Laboratory Medicine Vol.29 No.1

Background : Microdeletion syndromes not detectable by conventional cytogenetic analysis have been reported to occur in approximately 5% of patients with unexplained mental retardation (MR). Therefore, it is essential to ensure that patients with MR are screened for these microdeletion syndromes. Mental retardation syndrome multiplex ligation-dependent probe amplification (MRS-MLPA) is a new technique for measuring sequence dosages that allows for the detection of copy number changes of several microdeletion syndromes (1p36 deletion syndrome, Williams syndrome, Smith- Magenis syndrome, Miller-Dieker syndrome, DiGeorge syndrome, Prader-Willi/Angelman syndrome, Alagille syndrome, Saethre-Chotzen syndrome, and Sotos syndrome) to be processed simultaneously, thus significantly reducing the amount of laboratory work. Methods : We assessed the performance of MLPA (MRC-Holland, The Netherlands) for the detection of microdeletion syndromes by comparing the results with those generated using FISH assays. MLPA analysis was carried out on 12 patients with microdeletion confirmed by FISH (three DiGeorge syndrome, four Williams syndrome, four Prader-Willi syndrome, and one Miller-Dieker syndrome). Results : The results of MLPA analysis showed a complete concordance with FISH in 12 patients with microdeletion syndromes. Conclusions : On the basis of these results, we conclude that MLPA is an accurate, reliable, and cost-effective alternative to FISH in the screening for microdeletion syndromes. Background : Microdeletion syndromes not detectable by conventional cytogenetic analysis have been reported to occur in approximately 5% of patients with unexplained mental retardation (MR). Therefore, it is essential to ensure that patients with MR are screened for these microdeletion syndromes. Mental retardation syndrome multiplex ligation-dependent probe amplification (MRS-MLPA) is a new technique for measuring sequence dosages that allows for the detection of copy number changes of several microdeletion syndromes (1p36 deletion syndrome, Williams syndrome, Smith- Magenis syndrome, Miller-Dieker syndrome, DiGeorge syndrome, Prader-Willi/Angelman syndrome, Alagille syndrome, Saethre-Chotzen syndrome, and Sotos syndrome) to be processed simultaneously, thus significantly reducing the amount of laboratory work. Methods : We assessed the performance of MLPA (MRC-Holland, The Netherlands) for the detection of microdeletion syndromes by comparing the results with those generated using FISH assays. MLPA analysis was carried out on 12 patients with microdeletion confirmed by FISH (three DiGeorge syndrome, four Williams syndrome, four Prader-Willi syndrome, and one Miller-Dieker syndrome). Results : The results of MLPA analysis showed a complete concordance with FISH in 12 patients with microdeletion syndromes. Conclusions : On the basis of these results, we conclude that MLPA is an accurate, reliable, and cost-effective alternative to FISH in the screening for microdeletion syndromes.

자반고등어를 구우며

조은해 수필미학 2016 수필미학 Vol.14 No.-

The role of homologous recombination in ovarian cancer genetics

조은해 대한산부인과학회 2021 대한산부인과학회 학술대회 Vol.107 No.-

분홍색 환한 감옥

조은해 수필미학 2014 수필미학 Vol.5 No.-

PP 지지체를 이용한 옻/PDDA 복합 멤브레인의 비수계 레독스 플로우 배터리 응용연구

조은해,원종옥 한국공업화학회 2016 한국공업화학회 연구논문 초록집 Vol.2016 No.1

에너지 저장 시스템의 한 종류인 비수계 레독스 플로우 배터리는 친환경적이며 높은 안정성과 넓은 전압범위로 인하여 최근에 많이 주목 받고 있는 시스템이다. 이 배터리의 주요 구성요소인 멤브레인은 양극과 음극을 분리시킴과 동시에 높은 이온 선택 성을 가져야 하는 중요한 역할을 한다. 따라서 천연물질이며 내구성이 강한 옻, 이온 교환기를 갖고 있는 Poly (diallydimethyl ammonium chloride) [PDDA], 그리고 다공성 막인 Polypropylene [PP]를 지지체로 사용함으로써 높은 이온 전도도를 갖는 복합 멤브레인을 제조하였다. PP+옻/PDDA 멤브레인 (PDDA 중량 비 40wt%)의 경우, 우수한 이온전도도 1.3X10^-1 값을 갖는다. 또한 비수계 레독스 플로우 배터리의 결과로서, 상용화된 멤브레인인 Neosepta AHA는 22.8%의 에너지 효율을 갖는 것에 비해 PP+옻/PDDA 멤브레인은 42.1%로 약 2배 높은 우수한 에너지 효율을 보이는 것을 확인하였다.

시︱조은

조은 새얼문화재단 2004 황해문화 Vol.42 No.-

Detection of Isoniazid and Rifampicin Resistance by Sequencing of katG, inhA, and rpoB Genes in Korea

조은해,배혜경,강성기,이은희 대한진단검사의학회 2009 Annals of Laboratory Medicine Vol.29 No.5

Background : In Korea, tuberculosis is resistant to isoniazid (INH) and/or rifampicin (RIF) in more than 10% of cases. To prevent the spread of resistant Mycobacterium tuberculosis strains, it is crucial to develop more rapid resistance detection methods. Methods : To determine the feasibility of using direct sequencing for detecting INH- and RIF-resistant strains, the katG gene, the regulatory region of the inhA gene, and the 81-bp hot-spot region of the rpoB gene from 95 culture isolates and 46 respiratory specimens were sequenced. Total 141 culture isolates were classified by conventional drug susceptibility testing (DST) as INHR/RIFR (N=30),INHR/RIFS (N=23), INHS/RIFR (N=15), and INHS/RIFS (N=73). Results : Compared with phenotypic DST, the overall sensitivity and specificity of sequencing were 83.0% (44/53) and 96.6% (85/88), respectively, for INH resistance, and 93.3% (42/45) and 100% (96/96), respectively, for RIF resistance. The rates were similar between culture isolates and respiratory specimens. Interestingly, three specimens with inhA -15C>T mutation were susceptible to INH by conventional DST. Conclusions : Detection of mutations in the katG codon 315, the inhA regulatory region, and the hot-spot region of rpoB would be useful for rapid detection of INH and RIF resistance in Korea. Background : In Korea, tuberculosis is resistant to isoniazid (INH) and/or rifampicin (RIF) in more than 10% of cases. To prevent the spread of resistant Mycobacterium tuberculosis strains, it is crucial to develop more rapid resistance detection methods. Methods : To determine the feasibility of using direct sequencing for detecting INH- and RIF-resistant strains, the katG gene, the regulatory region of the inhA gene, and the 81-bp hot-spot region of the rpoB gene from 95 culture isolates and 46 respiratory specimens were sequenced. Total 141 culture isolates were classified by conventional drug susceptibility testing (DST) as INHR/RIFR (N=30),INHR/RIFS (N=23), INHS/RIFR (N=15), and INHS/RIFS (N=73). Results : Compared with phenotypic DST, the overall sensitivity and specificity of sequencing were 83.0% (44/53) and 96.6% (85/88), respectively, for INH resistance, and 93.3% (42/45) and 100% (96/96), respectively, for RIF resistance. The rates were similar between culture isolates and respiratory specimens. Interestingly, three specimens with inhA -15C>T mutation were susceptible to INH by conventional DST. Conclusions : Detection of mutations in the katG codon 315, the inhA regulatory region, and the hot-spot region of rpoB would be useful for rapid detection of INH and RIF resistance in Korea.