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LaM 상에서 암모니아와 메탄올로부터 메틸아민의 합성시 반응조건이 활성과 선택성에 미치는 영향
하백현,조원동,강동헌 한국화학공학회 1999 Korean Chemical Engineering Research(HWAHAK KONGHA Vol.37 No.4
수소형 모더나이트(HM)와 이를 lanthanum 이온으로 이온교환한 LaM를 만들고 그의 구조적인 특성변화를 XRD, 산성을 IR에 의하여 측정하고 암모니아와 메탄올로부터 메틸아민 반응에서 반응온도, 반응압력, 접촉시간 및 암모니아/메탄올의 비를 변화시키며 활성과 DMA의 선택성을 연구하였다. 두 시료의 경우 모두 활성과 선택성의 변화는 80h 내에서는 일어나지 않았으며 온도상승에 따라 활성이 증가하며 온도에 따라 전화율의 변화가 큰 영역에서 DMA의 선택성이 가장 크다. LaM이 HM보다 활성은 좀 떨어지나 DMA의 선택성비 커서 HM의 30%에 비해 약 최대 50%를 나타내었다. 암모니아/메탄올의 비의 증가에 따라 알코올의 전화율이 증가하고 space velocity의 증가에 따라서는 전화율이 감소하나 space velocity가 2-4h^(-1)이상에서는 거의 변화가 없었다. 반응압력의 증가는 MMA의 생성을 급격히 감소시키며 DMA의 선택성을 증가시켰으나 약 4atm 이상에서는 전화율에 크게 영향을 주지 못했다. Hydrogen mordenite(HM) was ion-exchanged with lanthanum to give LaM and these catalysts were characterized by using XRD for crystallinity and IR for acidities/structures of the catalysts. The effects of reaction conditions such as reaction temperature, pressure, contact time and ratio of ammonia/methanol on the catalytic activity of methylamine synthesis and the selectivity to dimethylamine(DMA) were investigated from ammonia and methanol. The catalytic activity and DMA-selectivity of these catalysts remained for 80 h, and maximum selectivities of DMA were obtained at temperature range of methanol was rapidly converted. The DMA selectivity on LaM was 50% which was larger than that of 30% obtained on HM. The conversion of methanol increased with the ratio of ammonia/methanol and decreased with space velocity, but for the space velocity, 2-4 h^(-1) it was constant. The elevation of reaction pressure rapidly reduced the amount of produced monomethlamine, but increased selectivity of DMA up to 4 atm.
Generation of 1E8 Single Chain Fv-Fc Construct Against Human CD59
홍정원,윤상순,조원동,홍권표,김솟을,손승명,윤석중,이호창,송형근 대한면역학회 2012 Immune Network Vol.12 No.1
Background: Therapeutic approaches using monoclonal antibodies (mAbs) against complement regulatory proteins (CRPs:i.e.,CD46,CD55 and CD59) have been reported for adjuvant cancer therapy. In this study, we generated a recombinant 1E8 single-chain anti-CD59 antibody (scFv-Fc)and tested anti-cancer effect.by using complement dependent cytotoxicity (CDC). Methods: We isolated mRNA from 1E8 hybridoma cells and amplified the variable regions of the heavy chain (VH) and light chain (VL) genes using reversetranscriptase polymerase chain reaction (RT-PCR). Using a linker, the amplified sequences for the heavy and light chains were each connected to the sequence for a single polypeptide chain that was designed to be expressed. The VL and VH fragments were cloned into the pOptiVEC-TOPO vector that contained the human CH2-CH3 fragment. Then, 293T cells were transfected with the 1E8 single-chain Fv-Fc (scFv-Fc) constructs. CD59 expression was evaluated in the prostate cancer cell lines using flow cytometry. The enhancement of CDC effect by mouse 1E8 and 1E8 scFv-Fc were evaluated using a cytotoxicity assay. Results: The scFv-Fc constructs were expressed by the transfected 293T cells and secreted into the culture medium. The immunoreactivity of the secreted scFv-Fc construct was similar to that of the mouse 1E8 for CCRF-CEM cells. The molecular masses of 1E8 scFv-Fc were about 120 kDa and 55 kDa under reducing and non-reducing conditions, respectively. The DNA sequence of 1E8 scFv-Fc was obtained and presented. CD59was highly expressed by the prostate cancer cell line. The recombinant 1E8 scFv-Fc mAb revealed significantly enhanced CDC effect similar with mouse 1E8 for prostate cancer cells. Conclusion: A 1E8 scFv-Fc construct for adjuvant cancer therapy was developed.
황정현,김현중,김현진,신나라,오석진,박정훈,조원동,안정오,BHATIA SHASHI KANT,양영헌 한국생물공학회 2024 Biotechnology and Bioprocess Engineering Vol.29 No.1
Seaweed biomass in Korea is rich in galactose following hydrolysis, and leveraging this resource for enhancing the biohydrogen production is the aim of this study. The study investigates the biohydrogen production potential of a newly isolated pure strain, Clostridium sp. JH03, utilizing galactose and seaweed biomass as renewable feedstocks. The strain could utilize galactose as the sole carbon source for biohydrogen production, with a maximum hydrogen yield of 1.61 mol H 2 /mol galactose. The parameters included pH, temperature, and initial galactose concentration, which were varied to determine the optimal conditions for maximum biohydrogen production. The optimal conditions for biohydrogen production were pH 9 and a temperature of 25 °C, with an initial galactose concentration of 10 g/L. Moreover, hydrogen production from seaweed hydrolysate by Clostridium sp. JH03 resulted in maximum production of 1.71 mol H 2 /mol galactose. The study also investigated that combining sludge, a common practice in dark fermentation, with JH03 increased biohydrogen production by up to 34%. By addressing the need for clean energy and reducing raw materials price using biomass, this study contributes to the advancement of sustainable and cost-compatible energy solutions.