Lactobacillus acidophilus 발효유가 흰쥐의 생체내 Cadmium 축적에 미치는 영향

조영채,전무형 한국수의공중보건학회 1994 예방수의학회지 Vol.18 No.3

In Vitro 자계(磁界) 측정에 의한 비소화합물의 폐포 Macrophage 독성 평가

조영채,Cho, Young-Chae 대한예방의학회 1999 Journal of Preventive Medicine and Public Health Vol.32 No.4

Objectives: This study was conducted to evaluate the cytotoxicity of gallium arsenide(GaAs), indium phosphide(InP) and indium arsenide(InAs) all of which are used a$ the semiconductor eletments in semiconductor industry. Methods: Cytotoxicity id the alveolar macrophage was evaluated by the measurement of in vitro magnetometry, LDH release assay and histological examination. Results: The relaxation curves by the in vitro magnetometry showed that GaAs has the cytotoxicity for the alveolar macrophage which is more significant in the higher dosages, while this cytotoxicity is not appeared in the groups added with InP or InAs or PBS. In the decay constant for two minutes after magnetization, GaAs-added groups showed a significant decrease with increasing doses, but both InP- and InAs-added groups did not show any significance. The LDH release assay showed a dose-dependent increasing tendency in the GaAs-, InP- and InAs-added groups. In terms of cellular morphological changes, GaAs-added groups revealed such severe cellular damages as prominent destructions in cell membranes and their morphological changes of nucleus, while InP- and InAs-added groups remained intact in intracellular structures, except for cytoplasmic degenerations. Conclusions: It is suggested that GaAs is more influential to cytotoxicity of alveolar macrophages than InP and InAs. 본 연구는 반도체 산업에서 반도체소자로서 주목받고 있는 GaAs, InP및 InAs의 세포독성을 평가하기 위해 햄스터의 폐포 대식세포를 사용하여 in vitro 자계 측정, LDH 활성치측정 및 세포의 형태학적 관찰 등을 검토하였다. 세포자계측정 결과 GaAs, InP 및 InAs첨가군 모두 대조군(PBS첨가군)에 비해 완화곡선이 유의하게 지연되었으며, 특히 GaAs 첨가군은 농도증가에 따라 용량의존적으로 완화곡선이 지연되는 경향이었다. 자화 후 2분간의 완화계수는 대조군에 비해 GaAs 첨가군은 농도증가에 따라 유의하게 낮아지는 용량의존성이 높은 경향이었으나, InP 및 InAs 첨가군에서는 모두 유의성이 인정되지 않았다. LDH활성치는 GaAs, InP 및 InAs첨가군 모두 용량 의존적으로 점차 높아지는 경향이었다. 세포의 형태학적 관찰소견은 GaAs첨가군에서는 용량의존적으로 세포막의 현저한 파괴, 핵의 형태적 변화 등 심한 세포장해가 유발된 반면, InP첨가군과 InAs첨가군에서는 세포내의 구조는 유지되었으나 세포질의 변성이 관찰되었다. 결과적으로 GaAs는 InP나 InAs보다 폐포 대식세포의 세포독성이 강한 것으로 보인다.

카드뮴에 폭로된 폐포된 폐포대식세포의 세포독성 평가를 위한 세포자계측정, LDH활성도 및 Apoptosis

조영채 한국환경보건학회 2000 한국환경보건학회지 Vol.26 No.4

To evaluate the cytotoxicity of cadmium compounds, this study was conducted to measure the in vitro magnetometry, LDH release and cellular apoptosis using alveolar macrophages of hamsters. A series of magnetometric measurements in cadmium-added groups showed a significant dose-dependent decay of the relaxation curves. The LDH release rates showed a dose-dependently increasing tendency as the dose gradually increased. The positive rates of apoptosis were significantly higher in cadmium-added groups than the control groups. Conclusively, the cytotoxicity increased in a dose dependent way as the concentration of cadmium added increased, which reflected in the decay of relaxation curve in magnetometry, and increased LDH release rate and positive rate of apoptosis.

탈구된 인공수정체 재고정술과 공막내고정술을 이용한 인공수정체 교환술 임상결과 비교

조영채,박정민 대한안과학회 2020 대한안과학회지 Vol.61 No.7

Purpose: To investigate the clinical outcomes of refixation of a dislocated intraocular lens (IOL) and IOL exchange with intrascleral fixation. Methods: We performed a retrospective study of 90 patients (91 eyes) who underwent refixation or exchange of IOLs from January 2014 to April 2019. The patients were divided into an ab externo scleral refixation group (group 1), an intrascleral refixation group (group 2), and an exchange with intrascleral fixation group (group 3). We evaluated the best-corrected visual acuity (BCVA), spherical equivalent, cylindrical power, intraocular pressure, and postoperative complications. Results: The BCVA was 0.10 ± 0.17 (group 1), 0.15 ± 0.29 (group 2), and 0.31 ± 0.52 (group 3) at 6 months after surgery. The BCVA change in group 3 was significantly greater than that in groups 1 and 2 (p = 0.018 and p = 0.046, respectively). The final BCVA was not significantly different among the groups (p = 0.422). The spherical equivalent was -1.26 ± 1.72 diopters (D) (group 1), -1.32 ± 2.09 D (group 2), and -0.17 ± 1.58 D (group 3) at 6 months after surgery, showing that group 1 and group 2 were more myopic than group 3 (p = 0.004 and p = 0.001, respectively). Haptic slippage was the most common complication. Conclusions: Refixation of dislocated IOLs and IOL exchange with intrascleral fixation did not differ significantly in terms of the final visual outcomes. Refixation was associated with more myopia and a higher risk of IOL dislocation or haptic slippage than exchange. 목적: 탈구된 인공수정체 재고정술과 공막내고정술을 이용한 인공수정체교환술에 대한 임상결과를 알아보고자 하였다. 대상과 방법: 2014년 1월부터 2019년 4월까지 탈구된 인공수정체 재고정술 또는 인공수정체교환술을 시행한 환자 90명(91안)을 대상으로 후향적 연구를 진행하였다. 기존 인공수정체로 외부 공막고정술을 시행한 군(1군), 기존 인공수정체로 공막내고정술을 시행한 군(2군), 공막내고정술을 이용한 인공수정체교환술을 시행한 군(3군)으로 나누어 최대교정시력, 구면렌즈 대응치, 난시값, 안압 및 합병증을 관찰하였다. 결과: 최대교정시력(logMAR)은 수술 6개월 후 1군에서 0.10 ± 0.17, 2군에서 0.15 ± 0.29, 3군에서 0.31 ± 0.52로 향상되었다. 최대교정시력의 변화는 1, 2군보다 3군에서 유의하게 높았으나(p=0.018, p=0.046), 최종 최대교정시력은 유의한 차이가 없었다(p=0.422). 구면렌즈 대응치는 수술 6개월 후 1군에서 -1.26 ± 1.72 diopters (D), 2군에서 -1.32 ± 2.09 D, 3군에서 -0.17 ± 1.58 D로 근시화되었고, 3군보다 1, 2군에서 유의하게 더 큰 근시값을 보였다(p=0.004, p=0.001). 합병증 중 지지부 미끄러짐이 가장 많았다. 결론: 탈구된 인공수정체 재고정술은 인공수정체교환술과 비교하여 최종 최대교정시력에서 유의한 차이가 없었다. 인공수정체교환술보다 재고정술에서 더욱 근시화되는 경향이 있었고, 인공수정체 탈구 또는 지지부 미끄러짐의 위험이 높았다.

흡수성 치주조직 재생 차폐막에 대한 비교연구

조영채,정다연,이득용 한국결정성장학회 2023 한국결정성장학회지 Vol.33 No.2

Absorbable periodontal tissue regeneration barrier membranes (total 6; domestic 4; import 2) were comparatively analyzed. In the case of the xenograft barrier membrane, the collagen product had excellent tensile strength but low strain,and the porcine pericardial membrane had good mechanical properties, but its thickness was too thick to control. The synthetic PLLA membrane manufactured by the electrospinning had a relatively low water absorption capacity. However,the hybrid barrier membrane was able to control mechanical properties and biocompatibility through proper mixing of synthetic polymer and natural polymer. DA02 (PLLA/gelatin), a newly developed hybrid absorbable periodontal tissueregeneration membrane that is entirely dependent on imports, can be applied to an absorbable periodontal tissue regeneration barrier membrane due to suitable mechanical properties and biocompatibility. 흡수성 치주조직 재생유도재(국내 4개, 국외 2개) 총 6개 제품을 비교 연구하였다. 이종소재 차폐막의 경우, 콜라겐 제품은 우수한 기계적 강도를 보였으나 연신율이 작았다. 돼지심막은 우수한 기계적 물성에도 불구하고 두꺼운 두께로 사용에 제약이 되었다. 전기방사법으로 제조한 독일 PLLA/콜라겐 하이브리드형 복합 차폐막은 강도값과 흡수도는 낮았지만, 합성 및 천연고분자를 적절히 혼합함으로써 원하는 특성의 기계적 물성과 생체적합성 구현이 가능하였다. 본 연구에서 DA02(PLLA/젤라틴) 하이브리드형 복합고분자 소재는 기계적 물성과 생체적합성이 적절하여 100% 수입에 의존하는 하이브리드형 흡수성 치주조직 재생 차폐막에 적용이 가능하였다.

Dimension Control of Perovskite for RGB Colors

조영채,김기환 한국고분자학회 2024 한국고분자학회 학술대회 연구논문 초록집 Vol.49 No.1

유리체절제술 후 소수성 표면을 가진 친수성 아크릴 단초점인공수정체의 지연성 혼탁

조영채,박정민 대한안과학회 2020 대한안과학회지 Vol.61 No.10

Purpose: To report a case of late opacification of a hydrophilic acrylic monofocal intraocular lens (IOL) with a hydrophobic surface (CT SPHERIS® 204) after a second vitrectomy in a patient with proliferative diabetic retinopathy. Case summary: A 50-year-old male with diabetes presented with decreased visual acuity in the right eye. He had undergone phacoemulsification and IOL (CT SPHERIS® 204) implantation, vitrectomy, endolaser photocoagulation, and intravitreal bevacizumab (Avastin®) injection due to vitreous hemorrhage in the right eye about 43 months prior. Four months after the surgery, presenting with recurrent vitreous hemorrhage and neovascularization of the angle, he underwent partial vitrectomy and intravitreal and intracameral bevacizumab (Avastin®) injection. The best-corrected visual acuity was 0.1 in the right eye when he presented with decreased visual acuity. Slit lamp examination of the right eye showed diffuse whitish granular opacity on the surface of the IOL. The IOL was explanted, together with the capsular bag, and examined using scanning electron microscopy and energy- dispersive X-ray spectroscopy. Conclusions: This case is the first to report late opacification of a hydrophilic, acrylic monofocal IOL with a hydrophobic surface, the CT SPHERIS® 204. It is necessary to be cautious in using this type of lens in patients with diabetic retinopathy. 목적: 증식당뇨망막병증 환자에서 2회 유리체절제술 후 소수성 표면을 가진 친수성 아크릴 단초점인공수정체 CT SPHERIS® 204에 지연성으로 혼탁이 발생한 증례를 보고하고자 한다. 증례요약: 50세 남자 환자가 우안 시력저하를 주소로 내원하였다. 환자의 기저 질환은 당뇨였다. 내원하기 약 43개월 전 우안 유리체출혈로 유리체절제술, 수정체초음파유화술 및 인공수정체(CT SPHERIS® 204)삽입술, 광응고술, 유리체강내 베바시주맙(Avastin®)주입술을 받았다. 수술 4개월 후 우안에 전방각 신생혈관과 유리체강내 재출혈이 있어 부분 유리체절제술, 전방 및 유리체강내 베바시주맙주입술을 시행하였다. 우안 시력저하로 내원했을 때 우안 최대교정시력은 0.1이었고, 세극등현미경검사상 인공수정체 광학부 표면에 과립성 백색 혼탁이 광범위하게 관찰되었다. 수정체낭과 함께 인공수정체를 제거하였고, 주사전자현미경과 에너지 분산 방사선분광법을 시행하였다. 결론: 본 증례는 소수성 표면을 가진 친수성 아크릴 단초점인공수정체 CT SPHERIS® 204에 지연성으로 혼탁이 발생한 최초의 보고이며, 당뇨망막병증 환자에서 이러한 유형의 인공수정체 사용에 주의할 필요가 있다.

Lactobacillus acidophilus 발효유가 cadmium투여된 흰쥐의 혈액상과 신장조직에 미치는 영향

조영채,전무형,장경수,Cho, Young-chae,Jun, Moo-hyung,Chang, Kyung-soo 대한수의학회 1994 大韓獸醫學會誌 Vol.34 No.4

To elucidate the protective effects of Lactobacillus acidophilus-fermented milk against cadmium toxicity, the effects of administration of L acidophilus-fermented milk on hematological values and histopathological changes in cadmium-treated rats were investigated. The experimental rats were divided into 2 groups that were consisted of the one group administered with cadmium alone, and the other group administered with cadmium mixed with the fermented milk. Each group was orally administered with different doses of cadmium such as $1.7{\mu}g/g$ bw/day, $3.4{\mu}g/g$ bw/day, $6.8{\mu}g/g$ bw/day, and $13.6{\mu}g/g$ bw/day, respectively, for 1 to 8 weeks. Hematological values and enzyme activities, histopathological changes of kidney tissues were examined for the experimental groups. The values of RBC, WBC, and Hb in the groups administered with cadmium mixed with the fermented milk showed no significant differences to those of the groups administered with cadmium alone, but Hct showed significant reducing values. The activities of glutamic oxaloacetic transaminase(GOT) and glutamic pyruvic transaminase (GPT) in serum were significantly reduced than those of the groups administered with cadmium alone, at the low dose of cadmium treated groups. But alkaline phophatase(ALP) and lactate dehydorgenase(LDH) were significantly reduced at the high dose of cadmium treated groups. In histopathological study, a severe acute tubular necrosis of the convoluted tubules and distalation of tubules were showed in the groups administered with cadmium alone, but the kidney tissues of the groups administered with cadmium mixed with the fermented milk were similar to those of the normal group. In conclusion, the above results would suggest that L acidophilus-fermented milk has reducing effects on cadmium toxicity, at the low dose of cadmium administration.

첩대요법으로 호전된 요추 추간판탈출증 환자 1례 보고

조영채,이시형,조태영,이병이,진성순,임형호,Cho, Young-Chae,Rhee, See-Hyung,Cho, Tae-Young,Lee, Byeong-Yee,Jin, Sung-Soon,Lim, Hyung-Ho 척추신경추나의학회 2006 척추신경추나의학회지 Vol.1 No.1

Objectives : The objective of this study is to evaluate the treatment effect of Taping therapy in Herniation of Inter-Vertebral Disc. Methods : Taping therapy were performed for 31 days ambulatory care in a patient who had Herniation of Inter-Vertebral Disc. Results : Physical examination of Lumbar Spine has improved, VAS were also decreased. Conclusion: Taping therapy were effective in reducing the symptoms. We think that it need the further study and clinical trial for Herniation of Inter- Vertebral Disc.

지연성으로 증상이 발현된 수정체 내 덱사메타손 삽입물

조영채,염명인,김상수 대한안과학회 2020 대한안과학회지 Vol.61 No.9

Purpose: To report a case of delayed symptoms after intralenticular dexamethasone implant (Ozurdex®; Allergan, Irvine, CA, USA). Case summary: A 65-year-old male was referred for myodesopsia and decreased visual acuity. A dexamethasone implant was located in the crystalline lens, and the lens showed mild nucleus opacity. The patient had been treated with multiple intravitreal injections of anti-vascular endothelial growth factor and had received an injection of dexamethasone implant about seven weeks before symptom onset. Based on the symptoms, phacoemulsification was performed and an intraocular lens was placed within the capsular bag. After surgery, symptoms improved. One month after surgery, as cystoid macular edema worsened, the patient was treated with an intravitreal injection of triamcinolone acetonide (MaQaid®; Wakamoto Pharmaceutical Co., Ltd., Tokyo, Japan). After intravitreal injection, cystoid macular edema improved. Conclusions: Intralenticular injection of the dexamethasone implant is very rare; however, great care should be taken during the procedure. It should be noted that the onset of symptoms by intralenticular dexamethasone implant may not occur immediately following the procedure. Thus, close follow-up is necessary, with the possibility of surgical intervention depending on visual acuity and underlying complications. 목적: 지연성으로 증상이 발현된 수정체 내 덱사메타손 삽입물(Ozurdex®; Allergan, Irvine, CA, USA) 1예를 경험하여 이를 보고하고자 한다. 증례요약: 65세 남자 환자가 우안 비문증과 시력저하를 주소로 내원하였다. 세극등현미경검사상 경도의 수정체핵 혼탁과 수정체 내덱사메타손 삽입물이 관찰되었다. 환자는 당뇨황반부종으로 수차례 유리체강내 혈관내피성장인자억제제 주사를 받았으며, 증상 발생 약 7주 전 덱사메타손 삽입물주입술을 받았다. 환자가 증상에 따른 불편감을 지속적으로 호소하여 수정체유화술과 후방인공수정체삽입술을 시행하였다. 수술 후 증상은 호전되었다. 수술 1개월 후 낭포황반부종이 악화되어 유리체강내 트리암시놀론 아세토나이드(MaQaid®; Wakamoto Pharmaceutical Co., Ltd., Tokyo, Japan) 주사를 시행하였다. 유리체강내 주사 후 낭포황반부종은 점차 호전되는 양상을 보였다. 결론: 수정체 내로 덱사메타손 삽입물을 주입하게 되는 경우는 매우 드물지만 시술 중 주의하여야 하며, 지연성으로도 수정체 내 덱사메타손 삽입물로 인한 증상이 발현될 수 있음을 인지할 필요가 있다. 따라서 면밀한 경과 관찰이 필요하며, 시력과 합병증에 따라 수술을 시행할 수 있다.