HPLC를 이용하여 토끼 적혈구막에서 ATP 가수분해에 관한 연구

조영욱,유미정,김창주,이원규,김정혜,호순태 최신의학사 1991 最新醫學 Vol.34 No.2

Generation of Nonclassical Narrowband Photon Pairs from a Cold Rubidium Cloud

조영욱,박광균,이종찬,김윤호 한국물리학회 2013 THE JOURNAL OF THE KOREAN PHYSICAL SOCIETY Vol.63 No.4

We report the generation of nonclassical narrowband photon pairs via the spontaneous fourwave mixing process in a cold Rubidium (87Rb) cloud prepared in a magneto-optical trap. The generated photon pairs exhibit strong violation of the Cauchy-Schwarz inequality by a factor of 625 ± 45, confirming nonclassical correlation between the photons. The biphoton wave packets,measured for several different experimental conditions, are in good agreement with the theoretical results.

Immunophenotypic Characterization and Quantification of Neoplastic Bone Marrow Plasma Cells by Multiparametric Flow Cytometry and Its Clinical Significance in Korean Myeloma Patients

조영욱,박찬정,박서진,지현숙,장성수,박상혁,서을주,윤덕현,이정희,서철원 대한의학회 2013 Journal of Korean medical science Vol.28 No.4

Multiparametric flow cytometry (MFC) allows discrimination between normal and neoplastic plasma cells (NeoPCs) within the bone marrow plasma cell (BMPC)compartment. This study sought to characterize immunophenotypes and quantitate the proportion of NeoPCs in BMPCs to diagnose plasma cell myeoma (PCM) and evaluate the prognostic impact of this method. We analyzed the MFC data of the bone marrow aspirates of 76 patients with PCM and 33 patients with reactive plasmacytosis. MFC analysis was performed using three combinations: CD38/CD138/-/CD45; CD56/CD20/CD138/CD19; and CD27/CD28/CD138/CD117. The plasma cells of patients with reactive plasmacytosis demonstrated normal immunophenotypic patterns. Aberrant marker expression was observed in NeoPCs, with negative CD19 expression observed in 100% of cases, CD56+ in 73.7%, CD117+ in 15.2%, CD27- in 10.5%, CD20+ in 9.2%, and CD28+in 1.3%. In PCM patients, more than 20% of NeoPCs/BMPCs were significantly associated with factors suggestive of poor clinical outcomes. Patients who were CD27- or CD56+/CD27-, demonstrated shorter overall survival than patients of other CD56/CD27combinations. Our results support the clinical value of immunophenotyping and quantifying NeoPCs in PCM patients. This strategy could help to reveal poor prognostic categories and delineate surrogate markers for risk stratification in PCM patients.

Rapid Detection of Prognostically Significant Fusion Transcripts in Acute Leukemia Using Simplified Multiplex Reverse Transcription Polymerase Chain Reaction

조영욱,Hyun-Sook Chi,박찬정,Seongsoo Jang,Eul-Ju Seo 대한의학회 2012 Journal of Korean medical science Vol.27 No.10

Multiplex reverse transcription polymerase chain reaction (mRT-PCR) has recently emerged as an alternative to cytogenetics. We designed and used simplified mRT-PCR system as a molecular screen for acute leukemia. Fifteen fusion transcripts were included: BCR-ABL1,PML-RARA, ZBTB16-RARA, RUNX1-RUNX1T1, CBFB-MYH11, DEK-NUP214, TCF3-PBX1,ETV6-RUNX1, MLL-AFF1, MLL-MLLT4, MLL-MLLT3, MLL-MLLT10, MLL-ELL, MLL-MLLT1,and MLL-MLLT6. A total of 121 diagnostic acute leukemia specimens were studied,comparing the mRT-PCR system with standard cytogenetics. Fifty-six cases (46.3%) had fusion transcripts revealed by our mRT-PCR assay. The concordance rate between mRT-PCR and cytogenetics was 91.7%. However, false negative results were found in three cases who have inv(16), t(4;11) or t(11;19)(q23;p13.1), respectively. Seven cryptic translocations including ETV6-RUNX1, MLL-MLLT3, MLL-MLLT4, and PML-RARA were detected. This mRT-PCR assay is a useful screening tool in acute leukemia because it provides rapid and reliable detection of clinically important chimeric transcripts. In addition, cryptic translocations provide additional genetic information that could be clinically useful.

중이 선종 1예

조영욱,김도윤,박홍주 대한이비인후과학회 부산,울산,경남 지부회 2012 임상이비인후과 Vol.23 No.2

Middle ear adenoma is a rare benign tumor of the tympanic cavity. The first report of a adenomatous tumor of middle ear was published in 1976 by Hyams. It can have both neuroendocrine and epithelial differentiation. Immunohistochemical techniques have helped clarify that these tumors all share elements of neuroendocrine differentiation. Due to non specific clinical symptom and imaging finding, preop-diagnosis was difficult. And behavior of middle ear adenoma was not predictable. In the past few years, there have been reports on metastasis of middle ear adenomas. In this study, we present a case of middle ear adenoma of a patient complaining non whirling type vertigo, hearing difficulty, which was diagnosed by histopathologic finding

미세혈관병용혈빈혈을 동반한 신장과 골수침범 경쇄침착질환 1예

조영욱,지현숙,박찬정,장성수,조영미,박정식 대한진단검사의학회 2009 Annals of Laboratory Medicine Vol.29 No.5

We report a case of light chain deposition disease in a 59-yr-old female showing deposition of monoclonal light chain in the kidney and bone marrow accompanied with a schistocytosis, the morphologic finding of microangiopathic hemolytic anemia. The immunofluorescence examination of the kidney revealed strongly stained κ-light chain deposits on the glomerular mesangium and capillary wall, tubules, and vessel wall. The electron microscopy demonstrated electron-dense deposits on the glomerular basement membrane and mesangium. Anemia was observed with schistocytosis and Howell-Jolly body in the peripheral blood smears. The immunohistochemical examination of the bone marrow showed the presence of κ-light chain deposits in scattered plasma cells and thickened vessel wall in the absence of a prominent plasma cell proliferation. Although an immunofixation electrophoresis failed to detect a monoclonal gammopathy, the presence of monoclonal protein could be identified by an abnormal κ/λratio on the serum free light chain analysis. 저자들은 신증후군으로 내원한 59세 여자 환자에서 신장과 골 수에 단클론성 경쇄가 침착되어 있고, 미세혈관병용혈빈혈(microangiopathic hemolytic anemia)의 형태학적 소견인 분열적 혈구증을 동반한 경쇄침착 질환(light chain deposition disease) 증례를 경험하였다. 환자의 신생검에서는 사구체와 세뇨 관의 기저막, 그리고 혈관벽에 면역형광염색에 강하게 반응하 는 κ형 경쇄가 침착되어 있었고, 전자현미경상 사구체 기저막과 사구체간질에 전자고밀도물질의 침착이 관찰되었다. 말초혈액 에서는 분열적혈구증과 Howell-Jolly 소체를 동반한 빈혈이 관 찰되었다. 골수생검에서는 형질세포의 현저한 증식은 없었으나, κ형 경쇄에 대한 면역조직화학염색에서 산재되어 있는 형질세 포와 비후된 혈관벽에 양성 소견을 보였다. 면역고정전기영동 에서 단클론감마글로불린병증의 증거는 없었으나, 혈청 유리형 경쇄 측정에서 비정상 κ/λ비로 단클론성 단백의 존재를 확인할 수 있었다.

B형 Niemann-Pick 병에 이환된 한국인 성인 1예: 이차성 Sea-blue 조직구증 및 분자적 특성 규명

조영욱,공수정,유한욱,채정돈,이원미,우정주,이홍복,박찬정,김구환 대한진단검사의학회 2009 Annals of Laboratory Medicine Vol.29 No.2

Niemann-Pick disease (NPD) is an inherited metabolic disorder caused by a deficiency of the enzyme acid sphingomyelinase coded by SMPD1 gene. In contrast with type A NPD, a severe neurodegenerative disease of infancy, type B NPD patients have little or no neurodegeneration, and frequently survive into adulthood. Although over 100 mutations have been found within the SMPD1 gene causing NPD, there was only one report about SMPD1 mutation status of a Korean NPD patient. We report a case of a 32-yr-old female, who presented with thrombocytopenia without any neurologic involvement. Hepatosplenomegaly was detected by both physical examination and imaging studies, and a thoracic radiograph examination showed a pattern of interstitial lung disease. Biochemical tests revealed increased liver enzymes, cholesterol, triglyceride, and LDL-cholesterol, and decreased HDL-cholesterol. Sea-blue or foamy vacuolated histiocytes occurred in bone marrow and liver. Sequencing analysis of SMPD1 using genomic DNA from peripheral leukocytes identified a compound heterozygote of two mutations at exon 2: p.E246K and p.A357V. The former is a known mutation in an Italian patient, and the latter has not been reported yet. She has received oral rosuvastatin to treat hyperlipidemia at a dose of 10 mg per day for 4 months. This is the second report in which the mutation of SMPD1 gene was detected in a Korean NPD patient. The active genetic analysis of SMPD1 gene in patients with typical findings of type B NPD would enable us to facilitate diagnosis as well as to accumulate data on molecular characteristics of Korean NPD patients. Niemann-Pick 병(Niemann-Pick disease, NPD)은 SMPD1 유전자에 의해 생성되는 효소인 산성스핑고마이엘린분해효소가 결핍되는 유전성 대사질환이다. 유아기의 심한 신경변성질환으 로 발현하는 A형 NPD와는 달리 B형 NPD 환자들은 신경변성이 없거나 경미하므로 성인기까지 생존하는 경우가 많다. 현재까지 100개가 넘는 SMPD1 돌연변이가 NPD를 유발하는 것으로 알 려져 있는데, 한국인 NPD 환자의 SMPD1 돌연변이에 대한 보 고는 1예에 불과하다. 증례는 32세 여자 환자로 신경학적 증상 없이 혈소판감소증을 주소로 내원하였다. 이학적 검진과 방사선 학적 검사에서 간비종대가 있었고, 흉부방사선검사에서는 간질 성폐질환에 합당한 소견이 관찰되었다. 생화학 검사에서는 간효 소, 총 콜레스테롤, 중성지방, LDL-콜레스테롤 증가, HDL-콜 레스테롤 감소 등을 보였고, 골수 및 간생검에서 sea-blue 조직 구 또는 공포성 포말조직구가 관찰되었다. 말초혈액 백혈구에서 추출한 genomic DNA를 이용한 SMPD1 유전자의 염기서열분 석 결과 엑손 2에서 p.E246K와 p.A357V 돌연변이가 복합이형 접합자로 확인되었다. 전자는 이탈리아 환자에서 보고된 바 있 으나, 후자는 아직 보고된 바가 없다. 환자는 고지혈증 치료를 위해 rosuvastatin을 1일 10 mg의 용량으로 4개월간 복용하였 다. 본 증례는 한국인 NPD 환자에서 SMPD1 유전자의 돌연변 이가 확인된 2번째 예이다. 앞으로는 B형 NPD의 특징적인 소 견을 보이는 환자들에서 적극적인 SMPD1 유전자 분석을 실시 해 B형 NPD의 진단을 촉진하고 한국인 NPD 환자군의 분자적 특성에 대한 자료를 축적해야 할 것이다.

이진탕 분획엑스가 synaptosomal NaK ATPase와 Ca ATPase의 활성도에 미치는 영향

조영욱,한승호,김창주,민병일,이태희,윤상협,오수명 대한한의학회 1997 대한한의학회지 Vol.18 No.1

말초혈액 도말검사로 우연히 진단된 말레이 사상충증 2예

조영욱,성흥섭,이호종,김지훈,석웅,박호선,정태은,이영하 대한진단검사의학회 2005 Annals of Laboratory Medicine Vol.25 No.1

The Brugian filariasis in Korea was once endemic in several areas including Jeju-do and small remote islands located in the southwestern part of the Korean peninsula. But recently it has almost been controlled. The first patient was a 42-year-old man, who visited the hospital with the chief complaints of fatigue, jaundice and edema on the right low extremity. Examination of a peripheral blood smear revealed non-megaloblastic macrocytic anemia, thrombocytopenia and eosinophilia, and a parasite, which was identified as microfilaria of Brugia malayi on the glass slide of blood obtained from the patient at midnight. The second patient was a 23-year-old man, who visited the hospital with the chief complaints of cough, sputum and fever. A parasite resembling that of the first patient was found in a peripheral blood smear accompaning neutrophilia. No more parasites, however, were found in the peripheral blood obtained from the patient at midnight. These two clinical cases of Brugian filariasis indicate that the clinical laboratory in Korea should be able to detect microfilariae for the diagnosis of filariasis. 우리나라에서 말레이 사상충증은 과거 제주도와 한반도 남서부의 낙도지역에서 유행한 적이 있었으나, 현재 대부분 지역에서현저히 감소하였다. 첫째 예는 42세된 남자로 피곤함, 황달 그리고 우측 발등의 부종을 주소로 내원하였다. 말초혈액 도말검사에서 비거대적혈모구 대적혈구성 빈혈, 혈소판감소증, 호산구증다증과 함께 기생충이 관찰되었다. 자정에 채취한 말초혈액에서 말레이 사상충의 미세사상충으로 동정하였다. 둘째 예는 2세 남자로 기침, 가래 그리고 발열을 주소로 내원하였다. 말초혈액 도말검사에서 호중구증가증과 함께 첫째 예와 유사한 기생충이 관찰되었다. 그러나 자정에 채취한 말초혈액에서는 기생충이 발견되지 않았다. 두 증례를 통해 앞으로 국내 검사실에서도 사상충증의 진단에 대한 지식이 필요할 것으로 사료된다.

Acuracy of Automated Urine analyzer (UF-1000i) in Detecting Dysmorphic RBC

조영욱,박세영,김영옥 대한내과학회 2017 대한내과학회 추계학술대회 Vol.2017 No.1