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Zebrafish 동물모델에서 human HtrA2의 expression system 정립에 관한 연구
조성원,박효진,김구영,남민경,김호영,고인호,김철희,임향숙,Cho, Sung-Won,Park, Hyo-Jin,Kim, Goo-Young,Nam, Min-Kyung,Kim, Ho-Young,Ko, In-Ho,Kim, Cheol-Hee,Rhim, Hyang-Shuk 한국생명과학회 2006 생명과학회지 Vol.16 No.4
Mitochondrial serine protease로 알려진 human HtrA2 (hHtrA2)는 apoptosis 유도 과정에서 중요한 역할을 담당하고 있을 뿐만 아니라 hHtrA2가 motor neuron degeneration과 관련이 있다는 최근 연구 결과가 있으나, hHtrA2의 생리적 기능은 아직 명확하게 밝혀져 있지 않다. 이와 같이 생체내에서 필수적인 업무를 담당하는 hHtrA2의 기능을 심도 있게 연구하기 위해서는 적절한 동물모델 시스템이 필요하나 이에 대한 연구도 미흡한 실정이다. 따라서 본 연구에서는 hHtrA2의 기능 분석을 위한 기본적인 실험으로 zebrafish라는 동물모델을 선택하여 hHtrA2의 발현 시스템을 정립하였다. 먼저 zebrafish에 hHtrA2를 발현시키기 위하여 zebrafish에서 일반적으로 사용되는 발현 시스템인 pCS2+ vector에 hHtrA2와 GFP를 cloning하고 plasmid를 HEK293 cell에 transfection한 후, hHtrA2-GFP fusion 단백질의 발현을 immunoblot과 immunofluorescence staining assay로 확인한 바 약 64 kDa의 hHtrA2 단백질의 발현을 확인할 수 있었다. Zebrafish에서 hHtrA2-GFP fusion 단백질의 발현양상은 immunofluorescence microscope으로 확인하였다. hHtrA2-GFP DNA와 mRNA를 zebrafish embyro에 microinjection하여 두 가지 component의 발현을 비교 분석한 결과, DNA는 dot 형태로 mRNA는 몸 전체에 퍼져보이는 형태로 발현 양상의 차이는 있었으나 둘 다 zebrafish embryo에서 잘 발현되는 것을 알 수 있다. 다음 DNA를 주 component로 microinjection하여 zebrafish embryo에서 발현을 확인한 결과 hHtrA2는 72 hpf 까지 발현이 지속되는 것을 확인하였다. 본 연구에서 정립한 hHtrA2의 zebrafish 발현 조건은 앞으로 zebrafish에서 hHtrA2의 생리적 기능을 심도있고 정확하게 연구하는 데 있어 기본적인 자료로 활용 할 수 있을 것이다. HtrA2/Omi, a mitochondrial trypsin-like serine protease, is pivotal in regulating apoptotic cell death. Several lines of recent evidence suggest that HtrA2 is associated with the pathogenesis of neurodegenerative disorders; however, the physiological function of HtrA2 still remains elusive. For studying physiological function of HtrA2 in depth, it is necessary to develop a suitable expression system in the model animal. We therefore utilized the zebrafish as a model animal to establish expression of human HtrA2 (hHtrA2) in vivo. For expression of mature HtrA2 as GFP fusion in zebrafish embryos, the HtrA2 (WT) or (S306A) cDNAs with the C-terminal GFP tag were inserted into the pCS2+ plasmid. Expression patterns of HtrA2 in HEK293 cells were first monitored by immunofluorescence staining and immunoblot assays, showing approximately 64 kDa of the HtrA2-GFP fusion proteins. Subsequently, the hHtrA2 plasmid DNA or in vitro transcribed mRNA was microinjected into zebrafish embryos. The expression patterns of HtrA2 in Zebrafish embryos were monitored by GFP fluorescence in 24 hours-post-fertilization (hpf). Although expression patterns of HtrA2-GFP in developing embryos were different between the injected DNA and mRNA, both nucleic acids revealed good expression levels to further study the physiological role of HtrA2 in vivo. This study provides a suitable condition for expressing hHtrA2 in the zebrafish embryos as well as a method for generating useful system to investigate physiological properties of the specific human genes.
400 kHz 페라이트 유도 결합 플라즈마를 위한 임피던스 매칭 네트워크
조성원,방진영,이영광,정진욱,Cho, Sung-Won,Bang, Jin-Young,Lee, Young-Kwang,Chung, Chin-Wook 한국반도체디스플레이기술학회 2007 반도체디스플레이기술학회지 Vol.6 No.2
Low frequency (400 kHz) Ferrite ICP has been proposed for large area processing. Because the coupling coefficient is close to 1, the transformer matching can be adapted to Ferrite ICP. The transformer matching system is simple. In this paper the new matching system by controlling the turns of transformer using relays is proposed for impedance matching. We developed a simple matching system and characterized it when the turns were changed. It was observed that the 2-channel relay is available for transformer matching.
조성원(Sung Won Cho),심찬섭(Chan Sup Shim),김진홍(Jin Hong Kim),이문성(Moon Sung Lee),이남민(Nam Min Lee) 대한소화기학회 1989 대한소화기학회지 Vol.21 No.4
Appendiceal mucocele is a descriptive term for an abnormal mucous accumulation distending the appendiceal lumen regardless the underlying cause. The incidence of mucocele in appendectomy specimens and autopsy series is about 0.25Z Its diagnosis has usually been made at laparotomy for a suspected appendicitis or incidentally during abdominal exploraton for another reason, and the preoperative diagnosis based on its signs, symtoms or conventional examinations is very rare. Recently, some reports announced the advent of ultrasonography and CT scan for making preoper- ative diagnosis of appendiceal mucocele. So we report a case of appendiceal mucocele with the preoperative diagnosis due to its characteristic ultrasonographic features.
조성원(Sung Won Cho),심찬섭(Chan Sup Shim),김진홍(Jin Hong Kim),이문성(Moon Sung Lee),김중원(Joong Won Kim) 대한소화기학회 1991 대한소화기학회지 Vol.23 No.1
Polymyositis is a disorder of unknown etiology characterized by an inflammatory destruction of striated skeletal muscles, which dominated by lymphocytic infiltration. But two major etiologic possibilities exist: a viral infection and an abnormal recognition of self by the immune system of the body. Recently, we experienced a case of polymyositis associated with chronic persistent hepatitis who had evidence of hepatitis B virus infection. This 61-year old male patient was complaint of generalized muscle weakness, swallowing difficulty and arthralgia on multiple joints. The patient revealed characteristic findings of polymyositis on the face and the skin of both fingers, EMG and skin biopsy specimen. Also, proved to be have chronic persistent hepatitis by liver biopsy, viral markers and liver function test. So we report it with a review of the literature.
조성원(Sung Won Cho),심찬섭(Chan Sup Shim),이상무(Sang Moo Lee),김재준(Jae Jun Kim) 대한소화기학회 1987 대한소화기학회지 Vol.19 No.1
Left sided appendix is relatively rare, and is usually associated with anomaly of intestinal rotation. Differential diagnosis of a left sided appendicitis from other acute abdomen is very difficult due to anomalous position of appendix. We report a case of left sided periappendiceal abscess which was associated with anomaly in the 2nd stage of rotation of midgut.
담관내압 검사로 진단된 Sphincter of Oddi Dysfunction
조성원(Sung Won Cho),심찬섭(Chan Sup Shim),김진홍(Jin Hong Kim),이문성(Moon Sung Lee),조영덕(Young Deok Cho) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.4
Biliary dyskinesia is a functional disorder of the biliary tract, especially spasm of the sphincter of Oddi(SO). It is sometimes impossible to differentiate patients with true biliary dyskinesia from those patients with papillary stenosis, which is a structural narrowing of part or all of the SO segment. Because of the difficutly in clearly separating these two clinical entities, this syndrome have been eailed SO dysfunetion. Classically, the patient with SO dysfunction is frequently a female between 20 and 50 years of age with a history of recurrent biliary-type pain. the discomfort is occasionally associated with nausea, vomiting, eructation and may be exacerbated by food intake. The diagnosi of SO dysfunction has been suggested by the symptom complex, exclusion of anatomic biliary tract disease, and finally by the pharmacologic provocation and relief of pain. Rer.ent developement in recording instrument such as biliary manometry allows the accurate evaluation of the motor activity of. the SO. In the treatrnent of SO dysfunction, endoscopic or surgical sphincterotomy has been used to abolish the sphincter mechanism, and pharmacologic means such as nifedipine may have a possible therapeutic rele. We report a case of biliary II type of SO dysfunction diagnonsed by biliary manornetry. The patient had sufferd from billiary-type pain for 5 years, and had normal liver function tests, delayed drainage of ERCP contrast beyond 45 minutes, and a dilated common bile duct. SO manometric pressure measurement revealed a signifieant elevation of SO basal pressure. The patient was treated by endoscopic sphinctecrotomy with a favorable manometric and symptomatic response.
조성원(Sung Won Cho),심찬섭(Chan Sup Shim),김진홍(Jin Hong Kim),이문성(Moon Sung Lee),임상복(Sang Bok Lim),최두홍(Du Hong Choi) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.2
Of all the many types of foreign bodies which may enter the upper gastrointestinal tract, some may cause serious complication while they are spontaneously passed. But objects longer than 10.0 cm and thicker than 2.0 cm tend to lodge in the stomach, duodenojejunal flexure, ileocecal valve and anus. We experienced a case of spontaneously passed long esophageal stent (13.0 * 2.5 cm in size), which was inserted because of lower esophageal stricture by extension from cardiac cancer of the stomach, after chemotherapy. So, we report this case with the review of the literature.
조성원(Sung Won Cho),심찬섭(Chan Sup Shim),김진홍(Jin Hong Kim),이문성(Moon Sung Lee),김홍수(Hong Soo Kim),김기업(Ki Up Kim) 대한소화기학회 1990 대한소화기학회지 Vol.22 No.4
The freqency of anatomical variatin of the bile duct system and its arterial supply are so common that a normal anatomical situation is encountered in only 35% of patients. While many of these variations have little or no clinical implications, some cases cause the disease, or may led to on incorrect diagonosis and treatment of biliary disorder. Diagnostic problems may arise from the false interpretation of biliary anomalies observed during cholecystography, ERCP and biliary endscopy. Accurate treatment of biliary disease, whether by surgeon endoscopist, must take account of variations smce accidental section or Jigatioon of aberrahf bile strietures or blood vessele can have a dramatic outcome, leading to sudden jaundice and/or bleeding. We present a very unusual case with the duplieation of a proximal portion of extrahepatic bile duct with multiple stones. The intrahepatrc bile ducts arise from right and Left lobe of the liver, wbich are not connected till they reach at the level of the distal ccimmon duct and the cystic duct draina to the right extrahepatic bile duct.
간장 ( 肝臟 ) · 담도 ( 膽道 ) 및 췌장 ( 膵臟 ) : 만성 췌장염에서의 췌관확장술
조성원(Sung Won Cho),심찬섭(Chan Sup Shim),김진홍(Jin Hong Kim),이문성(Moon Sung Lee),김홍수(Hong Su Kim),조영덕(Young Duk Cho) 대한소화기학회 1991 대한소화기학회지 Vol.23 No.4
N/A We report a case of chronic recurrent pancreatitis accompanied by multiple stenosis of the main pancreatic duct which successfully treated by endoscopic dilatation of pancreatic duct. Chronic pancreatitis with stenosis of pancreatic duct was diagnosed clinically and by endoscopic retrograde cholangiopancreatography. In order to relieve recurrent abdominal pain, we performed endoscopic pancreatic bougination with Soehenda biliary uilatation catheters and balloon dilatation with Rigiflex biliary balloon catheters. No significant complications occurred following pancreatic duct dilatation except mild pancreatitis associated with pancreatic manipulation. We conclude that endoscopic pancreatic duct dilatation as a method to treat chronic pancreatitis is encouraging, but large series and longer term follow-up are necessary before its efficacy can be determined.