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간생검조직에서 B 형 및 C 형 간염바이러스 유전자의 검출
전기엽(Ki Youp Jeon),최영숙(Young Suck Choi),조남훈(Nam Whoon Cho) 대한내과학회 1995 대한내과학회지 Vol.48 No.1
N/A Objectives: It is known that about 8% and 1% of the Korean population is chronic carriers of hepatitis B virus (HBV) and hepatitis C virus (HCV), respectively. And hepatitis B and C viruses are associated with chronic carrier state and may cause chronic liver disease and hepatocellular carcinoma. For further understanding of the relationship between viral infections and liver diseases, it may be necessary to evaluate the presence of HBV-DNA and HCV-RNA in the liver tissues and we tried polymerase chain reaction (PCR) to detect them. Methods: We determined the HBV-DNA by PCR and HCU-RNA by the reverse transcription-polymerase chain reaction (RT-PCR) in the liver biopsy specimens of two patients with chronic hepatitis, six patients with liver cirrhosis and two patients with hepatocellular carcinoma. Results: 1) In the two liver biopsy specimens of chronic B hepatitis, HBV-DNA were positive and HCV-RNA were negative. 2) In the six freshly frozen liver specimens of liver cirrhosis, all of the six showed HBV-DNA positive and three of them showed HCV-RNA positive. 3) In two patients of hepatocellular carcinoma, one of them showed HBsAg positive, anti-HCV positive in serologic test, HBV-DNA and HCV-RNA were positive. And the other one showed HBsAg positive, anti-HCV negative, HBV-DNA positive and HCV-RNA negative in the freshly frozen liver tissues. Conclusion: Our findings demonstrate the feasibility of single PCR methods for the detection of HBV sequences and the feasibility of reverse transcription-single PCR technique for the detection of HCV sequences in freshly frozen liver tissues. And these techniques may reveal valuable information on the diagnosis of HRV infection and HCV infection and the pathogenesis of chronic viral hepatitis.
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