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논 토양 조건에서 팥 유묘기의 생육특성과 단백질 발현 양상
정해룡,권수정,윤성현,박민영,부희옥,김학현,이문순,우선희 한국작물학회 2022 한국작물학회지 Vol.67 No.4
ABSTRACT Recently, the demand for the cultivation of upland soil has been increasing, and the rate of conversion of paddy soilinto upland soil is also increasing. Theincrease in uneven precipitation due to climate change has resulted in dramatic effects ofwaterlogging stress on upland crops. Therefore, the present study was conducted to investigate the changes in growth characteristicsand the expression patterns of proteins at the two-leaf stage of adzuki beans. The domestic cultivar, Arari (Miryang No. 8), was usedto test waterlogging stress. At the two-leaf stage of adzuki beans, plant height slightly decreased androot fresh weight showedsignificant changes after 3 days of waterlogging treatment. Chlorophyll content was also significantly different after 3 days ofwaterlogging treatment compared to its content in control plants. Using two-dimensional gel electrophoresis, more than 400 proteinspots were identified. Twenty-one differentially expressed proteins from the two-leaf stage were analyzed using linear trapquadrupole–Fourier transform–ion cyclotron resonance mass spectrometry. Of these 21 proteins, 9 were up-regulated and 12 weredown-regulated under waterlogging treatment. Protein information resource (https://pir.georgetown.edu/) categories were assignedto all 49 proteins according to their molecular function, cellular component localization, and biological processes. Most of theproteins were found to be involved in the biological process, carbohydrate metabolism and were localized in chloroplasts. 적 요논 토양 조건에서 팥의 생육기 중 초기 생육 단계에 해당되는 유묘기에 과습 스트레스 처리 후 생육특성 변화 및 팥잎의 단백질 발현 양상을 조사한 결과는 다음과 같다. 1. 아라리(밀양 8호)를 공시 품종으로 과습 스트레스 실험을 진행하였다. 3일간 과습 처리하는 동안 팥의 초장과생체중(잎, 줄기)은 통계적으로 유의하지는 않았으나 생체중(뿌리)와 SPAD value 값은 통계적으로 유의한 결과가 나왔다. 2. 과습 처리 3일 후 잎의 단백질 발현 양상을 확인한 결과21개의 차별 발현된 단백질 중 과습 스트레스 처리에 의해 9개의 단백질들이 up-regulated 되었고, 12개의 단백질들이 down-regulated 되었다. 단백질을 기능별로 분류한 결과 Biological Process에서 carbohydrate metabolicprocess와 관련된 단백질들이 가장 많이 나왔고 CellularComponent에서는 Chloroplast에서 가장 많이 존재하였다.
생부자(生附子) 및 삼부탕(蔘附湯)의 aconitine 함량 분석 및 삼부탕 단회투여 독성시험
정해룡,손변우,김경철,신순식,황원덕,Jeong, Hae-ryong,Son, Byun-woo,Kim, Gyeong-cheul,Shin, Soon-shik,Hwang, Won-deuk 대한한방내과학회 2016 大韓韓方內科學會誌 Vol.37 No.3
Objectives: This study investigated the aconitine contents analysis of Buja extracts (raw material of Buja, hot water extract of Buja, and hot water extract of Sambu-tang) and the single oral dose toxicity of Sambu-tang-R in six-week-old Sprague-Dawley rats in order to compare the toxicity of Buja extracts.Methods: Aconitine content analysis is that Buja extracts were hold purity test. To detect single oral dose toxicity, six-week-old Sprague-Dawley rats were divided into two groups, a normal control group and a sambutang-R (2,000 mg/kg) group. For 14 days of treatment, clinical signs, body weight, clinical chemistry, necropsy, and histopathology were examined.Results: The aconitine contents of the Buja extracts were Buja-RH (0.1738%), Buja-RD (0.1746%), and Sambu-tang-R (0.0961%). There were no cases of death in either the control group or the experimental group. Nor was there any disorder to the clinical signs or any significant change in body weight in either group. There was no significant change of clinical chemistry or disorder of necropsy findings in either the control or the experimental group. And there was no difference in histopathological findings in comparing the control group with the experimental group.Conclusions: These results suggest that the aconitine content of the hot water extract of Buja was similar to the raw material of Buja, but the hot water extract of Sambu-tang had greatly decreased aconitine content. These results also suggest that a single oral lethal dose of Sambu-tang-R for Sprague-Dawley rats exceeds 2,000 mg/kg for both female and male rats.