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Microsatellite DNA 를 이용한 연어(Oncorhynchus keta)와 곱사연어(O. gorbuscha)의 구분
정웅식,이윤호,신형철,성기백,김수암 한국수산자원학회 2001 한국수산자원학회지 Vol.4 No.-
본 연구에서는 형태적으로 구분이 불가능한 유통과정의 연어(Oncorhynchus keta)와 곱사연어(O. gorbuscha)를 구분하기 위하여 microsatellite DNA Ogo5와 Ogo6를 분석하여 비교하였다. 연어의 Ogo5 염기서열 반복구조는 네 가지 allele이 있었으며 곱사연어와 차이가 있었다: allele A, (GT)_2 GCATGC(GT)_2GCAC(GT)_3TCGGTTGCTTTT: allele B1, (GT)_2GTGTTT (GT)_2GCAT(GT)6TTTTTT; allele B2 (GT)_2 GTGTTT (GT)_2GCAT(GT)_7TTTTTT; allele B3 (GT)_2GTGTTT (GT)_2GCAT(GT)_7TTTTTTT: 곱사연어, (GT)_2GTGTTT (GT)_2GCAT(GT)_8TTTT. Ogo6 역시 연어와 곱사연어 사이에 반복구조의 길이에서 뚜렷한 차이를 보였다: 연어, (CA)_5AA(CA)_5CG와 CTCA(CT)_2GT; 곱사연어, (CA)_5AA(CA)_6AA(CA)_5CG와 CTCA(CT)_3GT. 또한, Ogo5와 Ogo6 염기서열은 두종 사이에 서로 다른 염기(single nucleotide polymorphism: SNP)가 각각 3개와 1개 있는 것으로 드러났다. 이러한 연구결과는 microsatellite DNA Ogo5와 Ogo6가 연어와 곱사연어를 형태적 구분이 불가능한 상태에서도 종 구분할 수 있는 유전자 마커로 사용될 수 있음을 시사한다. 이와 같은 유전학적 접근방법은 연어뿐만 아니라 시장에서 유통되는 다양한 수산물의 종과 원산지를 확인하는데 적용될 수 있다. Although the live chum salmon (Oncorhynchus keta) is discernable from the pink salmon (O. gorbuscha), processed salmon meat in the market place is hard to be distinguished from one species to the other. In order to resolve this situation, we analyzed microsatellite DNA Ogo5 and Ogo6 of the chum salmon and compared their sequences with those of the pink salmon. Four alleles of Ogo5 were identified in the chum salmon, which have distinct SNPs(Single Nucleotide Polymorphisms) and different numbers of repeat of (GT)n from the pink salmon: allele A. (GT)_2GCATGC(GT)_2GCAC(GT)_3TCGGTTGCTTTT: allele B1, (GT)_2GTGTTT(GT)_2GCAT(GT)_6TTTTTT: allele B2 (GT)_2GTGTTT(GT)_2GCAT(GT)_7TTTTTT: allele B3 (GT)_2GTGTTT(GT)_2GC AT(GT)_7TTTTTTT: the pink salmon, (GT)_2GTGTTT(GT)_2GCAT(GT)_8TTTT. Ogo6 of the chum salmon also has a distinct SNP and different numbers of repeats of (CT)n from that of the pink salmon: the chum salmon, (CA)_5AA(CA)_5CG and CTCA(CT)_2GT: the pink salmon, (CA)_5AA(CA)_6 AA(CA)_5CG and CTCA(CT)_3GT. These results suggest that the microsatellite DNA Ogo5 and Ogo6 would be good means of discrimination for the salmon meat in the market. Use of genetic markers as shown in this study could be applied to many different kinds of fish products for the identification of the species and their origins.
남극 호냉성 미세조 Porosira pseudodenticulata의 형태와 분자적 자료
정웅식,주형민,홍성수,강재신,최한구,강성호,Jung, Woong-Sic,Joo, Hyoung-Min,Hong, Sung-Soo,Kang, Jae-Shin,Choi, Han-Gu,Kang, Sung-Ho 한국조류학회(藻類) 2006 ALGAE Vol.21 No.2
We have cultured more than 100 Arctic and Antarctic cryophilic microalgal strains in KOPRI culture collections of polar microorganisms (KCCPM). Among them, we tried to identify an Antarctic strain, KOPRI AnM0008 by morphological and molecular analysis. Nuclear SSU rDNA and plastid rbcL sequences were used to identify the strain. It was identified as Porosira pseudodenticulata based on SSU sequence data showing 99% identity with GenBank X85398. This result was supported by morphological features like solitary labiate process, external foramina and internal cribra by optical and scanning electron microscope. Morphological identification and molecular analysis on polar cryophilic microalgae will be accomplished to construct the databases for KCCPM.
Cellular growth and fatty acid content of Arctic chlamydomonadalean
정웅식,김은재,임수연,심현지,한세종,김상희,강성호,최한구 한국조류학회I 2016 ALGAE Vol.31 No.1
Arctic microalgae thrive and support primary production in extremely cold environment. Three Arctic green microalgal strains collected from freshwater near Dasan Station in Ny-Alesund, Svalbard, Arctic, were analyzed to evaluate the optimal growth conditions and contents of fatty acids. The optimal growth temperature for KNF0022, KNF0024, and KNF0032 was between 4 and 8°C. Among the three microalgal strains, KNF0032 showed the maximal cell number of 1.6 × 107 cells mL−1 at 4°C. The contents of fatty acids in microalgae biomass of KNF0022, KNF0024, and KNF0032 cultured for 75 days were 37.34, 73.25, and 144.35 mg g−1 dry cell weight, respectively. The common fatty acid methyl esters (FAMEs) analyzed from Arctic green microalgae consisted of palmitic acid methyl ester (C16:0), 5,8,11-heptadecatrienoic acid methyl ester (C17:3), oleic acid methyl ester (C18:1), linoleic acid methyl ester (C18:2), and α-linolenic acid methyl ester (C18:3). KNF0022 had high levels of heptadecanoic acid methyl ester (26.58%) and heptadecatrienoic acid methyl ester (22.17% of the total FAMEs). In KNF0024 and KNF0032, more than 72.09% of the total FAMEs consisted of mono- and polyunsaturated fatty acids. Oleic acid methyl ester from KNF0032 was detected at a high level of 20.13% of the FAMEs. Arctic freshwater microalgae are able to increase the levels of polyunsaturated fatty acids under a wide range of growth temperatures and can also be used to produce valuable industrial materials.