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Cloning and Characterization of a Putative UDP-Rhamnose Synthase 1 from Populus euramericana Guinier
김봉규,정우담,안중훈 한국식물학회 2013 Journal of Plant Biology Vol.56 No.1
L-Rhamnose is a constituent of plant primary cellwall polysaccharides including rhamnogalacturonan-I,rhamnogalacturonan-II, and other natural plant-basedcompounds. UDP-rhamnose serves as a rhamnose donorwhose synthesis is catalyzed by UDP-rhamnose synthase(RHM). A RHM gene, PRHM was cloned from Populuseuramericana Guinier. PRHM contains two domains: theNAD dependent epimerase/dehydratase family domain andthe RmlD (dTDP-keto-rhamnose-4-keto-reductase) substratebindingdomain. Because the recombinant PRHM did notdemonstrate any activity during an in vitro assay,complementation with an Escherichia coli mutant wascarried out. The rfbD (dTDP-4-dehydrorhamnose reductase),which encodes an enzyme catalyzing the conversion ofdTDP-4-keto-rhamnose to TDP-rhamnose, was mutated inE. coli. The mutant strain B-rfbD was transformed withPRHM gene and a flavonoid rhanmosyltransferase gene,AtUGT78D1. The resulting transformant was able to convertquercetin into quercetin 3-O-rhamnoside in a manner similarto that by the wild type E. coli strain harboring AtUGT78D1. This result indicated that PRHM catalyzed the conversion ofUDP-glucose into UDP-rhamnose.
급경사지 산림작업을 위한 차량의 역학분석에 관한 연구 - 차륜형 집재작업 트랙터를 중심으로 -
정주상(Joo Sang Chung),정우담(Woo Dam Chung) 한국산림과학회 1995 한국산림과학회지 Vol.84 No.2
In this paper, a mechanical analysis model for steep - slope log - skidding operations of a rubber - tired tractor is discussed and the applicability of the model is investigated. The model largely consists of mathematical analysis models for log drag, dynamic vehicle weight distributions and soil - vehicle traction. For the case study, a theoretical data set for log skidding operations is used in investigating the effect of the factors influencing the results of mechanical analysis or the productivity of skidding operations. The analyses include 1) the effect of log choking methods on tangential log - skidding force, 2) the effects of the change in travel speed and log load on the required input power to the wheels and 3) the log skidding performance of a two - wheel drive compared with that of a four - wheel drive.
정주상(Joo Sang Chung),정우담(Woo Dam Chung) 한국산림과학회 1995 한국산림과학회지 Vol.84 No.3
A user - oriented computer model to aid designing forest road is developed to release the burden by increasing efficiency of time - consuming and laborious road design works. In this paper, the structures and functions of the model are discussed. The model consists of functional modules 1)input module to treat survey data and design criteria : 2)road design module to generate preliminary road layout, horizontal and vertical curvatures and curve widening : 3)earthwork analysis module to determine the economic mass movement : 4)report writer module to produce hard copies of engineering drawings for plan views, cross - sections and profiles, earthwork calculation sheets and mass movement diagrams. In addition, the report writer also provides the information on earthwork disturbance along the cut and - fill slopes. The modules are designed to be fully - integrated to enable the users to perform engineering analyses and evaluate design alternatives in a series of road design procedures.
유동식 가선집재시스템 Live Skyline 의 역학분석에 관한 연구
정주상(Joo Sang Chung),정우담(Woo Dam Chung) 한국산림과학회 1994 한국산림과학회지 Vol.83 No.3
In this paper, the characteristics of cable logging systems, of which skyline length is adjustable during operation are discussed from a standpoint of system geometry. Among the cable logging systems, the live skyline is the typical one, of which operation is easy to understand. To analyze the cable logging mechanics of the live skyline, computer models were developed. In developing the computer models, the concept of the live skyline operation was classified into `true live skyline` and `live skyline with lift`. The former assumes to adjust skyline length continuously to maintain the skyline clearance over ground constant during inhaul ; the latter assumes to shorten the skyline length only to keep the minimum skyline clearance during inhaul. The computer model was used in investigating the mechanical efficiency and the operational characteristics of the live skyline system. First, the payload capability of live skyline was compared with that of standing skyline. Second, the effect of the change in the log-drag geometry on the payload capability of live skyline was investigated. Finally, the payload capability of live skyline with lift was compared with that of true live skyline as an effort to explain the concepts of true live skyline and live skyline with lift.