수준별 과업 중심의 수업을 통한 초등영어 읽기 쓰기 지도와 효과 분석

정행 ( Haeng Jung ),노영신 ( Young Shin Noh ) 21세기영어영문학회 2009 영어영문학21 Vol.22 No.4

This paper aims to examine the effects of level-differentiated-task-based teaching on the development of primary school students` English reading and writing skills. A total of 75 sixth graders (38 students of a control class, 37 students of an experimental class) were participated in the study. They were divided into an advanced group and a remedial group in a class, respectively. Each group of the experimental class was taught for 10 weeks (twice a week) through different reading and writing tasks for this study which were developed to fit for the students` different levels. To find out the results of the study, reading and writing tests, a questionnaire, and self-evaluation were administered at the end. The findings of the study are as follows; 1) level-differentiated teaching had a positive effect on the literacy development of the advanced group while it didn`t influence the learning of the remedial group. 2) level-differentiated teaching contributed a lot to the students` interests and confidence in English in an advanced group but not in a remedial group.

인공피부모델 KeraSkinTM을 이용한 유전독성 평가

이수현 ( Su-hyon Lee ),정행선 ( Haeng-sun Jung ),김설영 ( Seol-yeong Kim ),김혜수 ( Hye Soo Kim ),임경민 ( Kyung-min Lim ),정영신 ( Young-shin Chung ),최태부 ( Tae-boo Choe ) 대한화장품학회 2016 대한화장품학회지 Vol.42 No.3

소핵시험은 세포분열 단계 중 간기 세포의 세포질 내 소핵 유무를 조사함으로써 유전독성을 평가하는 시험법이다. 최근 화장품 안전성 평가에 동물실험을 금지하거나 최소화하려는 노력이 확산되고 있어 유전독성 평가에 있어서도 기존의 동물실험이 아닌 새로운 in vitro 시험법이 요구되고 있다. 본 연구에서는 3차원 배양인공피부모델인 KeraSkin^TM을 이용하여 도포 처치된 물질의 유전독성을 평가하였다. 2종의 유전독성물질인 mitomycin C (MMC)와 methyl methanesulfonate (MMS)는 농도 의존적으로 세포독성과 소핵 형성이 유도된 반면, 대조물질인 4-nitrophenol (4-NP)와 trichloroethylene (TCE)에서는 농도 의존적으로 세포독성은 관찰되었으나 소핵은 형성되지 않았다. 따라서 인공피부모델을 이용한 소핵시험이 화장품과 같은 피부적용물질의 in vitro 유전독성 평가에 유용할 것으로 사료된다. Micronucleus test is genotoxicity assay for detection of micronuclei in the cytoplasm of interphase cells. The reduction and replacement of in vivo toxicity testing on animals require the development of in vitro models to predict the genotoxicity or other tests for cosmetic products. In this study, we evaluated a genotoxicity assay for topically applied chemicals using a three-dimensional human reconstructed skin model, KeraSkinTM. Two genotoxins, mitomycin C (MMC) and methyl methanesulfonate (MMS), induced significant dose-related increases in cytotoxicity and micronuclei induction in the skin model. In contrast, two non-genotoxins, 4-nitrophenol (4-NP) and trichloroethylene (TCE), induced cytotoxicity but not micronucleus formation. In conclusion, micronucleus test using human skin model may be useful for predicting in vitro genotoxic potentials of cosmetic products.

도라지 잎 에탄올 추출물의 주요 성분 분석 및 마우스 대식세포와 인체 폐암세포에서 항염효과

이정민,배병준,최지림,정영신,Jung Min Lee,Byeong Jun Bae,Jee-Lim Choi,Young-Shin Chung 한국식품영양학회 2024 韓國食品營養學會誌 Vol.37 No.2

This study investigated major constituents and anti-inflammatory effects of an ethanol extract of Platycodon grandiflorum leaves. Through HPLC analysis, chlorogenic acid and luteolin-7-O-glucoside were identified as predominant constituents in the ethanol extract. Their anti-inflammatory effects were evaluated using murine macrophage (RAW 264.7 cells) and human lung carcinoma cells (NCI-H292 & A549). The ethanol extract significantly (p<0.01) inhibited the production of nitrite, interleukin-6 (IL-6), and prostaglandin E2 (PGE2) induced by lipopolysaccharide (LPS) in RAW 264.7 cells. Furthermore, the ethanol extract suppressed the expression of cyclooxygenase-2 (COX-2) and inducible NO synthase (iNOS) proteins in RAW 264.7 cells stimulated with LPS. In NCI-H292 and A549 cells, treatment with the ethanol extract significantly (p<0.05) decreased levels of pro-inflammatory cytokines IL-6 and IL-8 induced by IL-1β. The phosphorylation of ERK rather than JNK in the mitogen-activated protein kinase signaling pathway was observed to be a more important mediator in the down-regulation of pro-inflammatory cytokines in NCI-H292 cells. These findings suggest that the ethanol extract of Platycodon grandiflorum leaves containing luteolin-7-O-glucoside exhibits promising anti-inflammatory properties.

두릅나무와 황백피의 혼합추출물 P55A 의 랫트 및 개에 대한 경구투여 급성독성

강부현(Boo Hyon Kang),손화영(Hwa Young Son),송시환(Si Whan Song),차신우(Shin Woo Cha),서동욱(Dong Oook Seo),이상봉(Sang Bong Lee),정영신(Young Sin Jung),홍은경(Eun Kyung Hong),김해리(Har Riet Kim),김성진(Sung Jin KIm) 한국응용약물학회 1999 Biomolecules & Therapeutics(구 응용약물학회지) Vol.7 No.2

The current study was performed to determine the acute oral toxicity of P55A, a crude extract of 1:1 mixture of Phellodendron amurense cortex and Aralia elate cortex, in SD rats and beagle dogs. 5 rats of each sex were treated with a single dose of P55A orally at doses of 0 and 5,000 mg/kg respectively. Also 2 dogs of each sex were treated with a single dose of P55A orally at doses of 0 and 2,000 mg/kg, respectively. After the treatment, clinical signs, and body weight change were observed for 14 days. All rats survived during the study and did not show any clinical sign. Body weight gain showed no significant difference between the control and treated rats. Grossly, no lesion was observed in the rats. All dogs survived during the study. In clinical signs, dark stool was observed in the 2,000 mg/kg treated dogs at day 1 after administration. The animals recovered from general signs at day 2 after administration. Body weight gain showed no significant difference between the control and treated dogs. Grossly, no lesion was observed in the dogs. It is suggested that the LD_(50) of P55A by oral administration was estimated to be over 5,000 mg/kg in both sexes of rats and 2,000 mg/kg in both sexes of beagle dogs.

새로운 항암제 AG 60 의 일반약리작용

성연희(Yeon Hee Seong),강종구(Jong Koo Kang),안희열(Hee Yul Ahn),김선돈(Sun Don KIm),이선애(Sun Ae Lee),조순옥(Soon Ok Jo),유보림(Bo Rim Yoo),정영신(Young Shin Jung),홍은경(Eun Kyung Hong),한영복(Young Bok Han) 한국응용약물학회 1997 Biomolecules & Therapeutics(구 응용약물학회지) Vol.5 No.4

General pharmacological properties of AG 60 (mixture of acriflavine and guanosine (1:1, w/w)), which has anticancer effect, following intramuscular administration were examined in terms of effects on central nervous system, gastrointestinal system, cardiovascular system, respiratory system and autonomic nervous system in mice, rats, guinea-pigs and rabbits. AG 60 at the dose of 15 mg/kg had no influences on pentobarbital sleeping time, spontaneous motor activity, chemoshock produced by pentylenetetrazole solution, writhing syndromes induced by 0.8% acetic acid solution, and motor coordination of mice. However, AG 60 at the dose of 7.5 and 15 mg/kg caused significant decrease of normal body temperature 1 and/or 2 h after the administration. No influence on body temperature was observed at 3.75 mg/kg in mice. Gastric secretion of rat and intestinal motility of mice were not influenced by the dose of 15 mg/kg. In terms of autonomic nervous system, AG 60 did not show direct effect and inhibitory or augmentative action of histamine- or acetylcholine-induced contractions at the concentration of 5 mg/L in the isolated ileum of guinea-pig. The administration of 15 mg/kg of AG 60 did not affect mean arterial blood pressure and heart rate in rat. AG 60 (15 mg/kg) given to anesthetized rabbits showed no effect on respiratory rate.

골수 유래 기질 줄기세포의 탐식작용 매개성 케모카인 수용체 발현 연구

정영신,변향민,신지영,김정목,정형민,오유경 한국약제학회 2003 Journal of Pharmaceutical Investigation Vol.33 No.4

To design gene delivery systems which can deliver higher amounts of genes into stem cells, we studied the expression of receptors involved in the receptor-mediated endocytosis of bone marrow stromal stem cells. Bone marrow was isolated from ICR mice, and bone marrow stromal stem cells were isolated based on their plastic adherence property. Several cultrure conditions were screened for effective and continuous culture of marrow stromal stem cells. MesenCult medium was finally used to cultivate marrow stromal stem cells in vitro. As candidate receptors, various chemokine receptors were studied. Both bone marrow cells and marrow-derived stromal stem cells showed expression of CC chemokine receptors (CCR) and CXC chemokine receptors (CXCR). Marrow stromal stem cells showed higher expression of CCR5 and CXCR4 chemokine receptors as compared to other types of chemokine receptors. Moreover, though the expression chemokine receptors generally decreased in most chemokine receptors with the cultivation of marrow stromal stem cells, CCR5 and CXCR4 chemokine receptors retained the higher lever of receptor expressions over prolonged periods. These results suggest that the ligands exhibiting specific binding to CCR5 or CXCR4 might be used to modify gene delivery systems for increased level of receptor-mediated gene delivery into stromal stem cells.