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정규회(Kyu Hoi Chung),계명찬(Myung Chan Gye),송재영(Jae Young Song) 한국환경생물학회 2000 환경생물 : 환경생물학회지 Vol.18 No.1
Herpetofauna biodiversity was surveyed at the Chin-do. The results are as follows: During census periods presence of 2 orders, 4 families, 6 species of amphibians and 1 order, 2 suborders, 4 families, 8 species of reptiles were observed or found indirectly by auditorial recording from residents at the Chin-do. Among them, 1 protected wildlife(Agkistrodon saxatilis) and several rare species such as Takydromus wolteri, Scincella laterale laterale and Zamenis spinalis were indentified. Rana nigromaculata and Rana catesbeiana were found in all surveyed areas. Dominant amphibian species was Rana rugosa (D´=25.8%) and reptile was Takydromus wolteri (D´=21.8%). Among 4 areas survey area, Uisin-myon showed the highest level of species richness, diversity, and evenness indices(R´=2.393, H´=1.931, E´=0.7772, respectively). The species richness of Kunnae-myon was lower(R´=0.932) than other surveyed regions. Based on the frequency of occurrence(85.71%), habitation density of Rana catesbeiana was very common in Chin-do, suggesting that spreading of this foreign species in the entire Korean peninsula. Scincella laterale laterale, Elaphe dione, Elaphe rufodorsata, Dinodon rufozonatus rufozonatus, Rhabdophis tigrinus tigrinus, Agkistrodon ussuriensis, and Zamenis spinalis showed low frequency of occurrence(<10%) and efforts to protect them are required. [Herpetofauna, Biodiversity, Chin-do].
정규회(Kyu Hoi Chung),심재한(Jae Han Shim),송재영(Jae Young Song) 한국환경생물학회 2001 환경생물 : 환경생물학회지 Vol.19 No.4
N/A Ecological measurement and biological environment of the Moak Provicial Park surveyed based on the habitat; deciduous broad leaved forest(Quercus mongolica variabilis-Pinus densiflora, Capinus tschonoskii, Quercus mongolica, Quercus acutissima-Pinus densiflora, and Quercus variabilis), coniferous forest(Pinus densiflora), plantation forest(Pinus rigida), and the other place(pond, lake, rice field, stream, and river). Totally, 1 orders, 4 families, 8 species of amphibians were found at Mt. Moak provincial park. Biodiversity indices of studied region were as follow; richness was 1.270, diversity was 1.775, and evenness was 0.853 in amphibians, 42.1% of amphibians were found in deciduous broad leaved forest, 11.3% in coniferous forest, 1.6% in plantation forest, and 45.0% in the other region.
Granulocyte Macrophage Colony Stimulating Factor에 의한 생쥐 초기 배아 발생의 신호전달
서혜영,정규회,강병문,계명찬,Suh, Hye-Young,Chung, Kyu-Hoi,Kang, Byung-Moon,Gye, Myung-Chan 대한생식의학회 2003 Clinical and Experimental Reproductive Medicine Vol.30 No.1
Objective: Present study was aimed to verify the effect of granulocyte macrophage-colony stimulating factor (GM-CSF) in the preimplantation development of mouse embryos and the involvement of the mitogen activated protein kiase (MAPK) in the GM-CSF signaling. Methods: Two-cell embryos were cultured for 96 h in the presence or absence of GM-CSF (0, 0.4, 2, 10 ng/ml) and PD98059, a MEK inhibitor (10 ${\mu}M$). Morphological development, cell number per blastocyst, and apoptotic nuclei, were eamined. MAPK activity of embryonic immunoprecipitate by MAPK (ERK1/2) antibody was measured by in vitro phosphorylation of myelin basic protein. Results: At post hCG 122 h the embryonic development among the experimental groups was significantly different (p=0.018). The rate of blastocyst development and cell number per embryo were the highest in 2 ng/ml GM-CSF treatment group. The percent of apoptotic cells of the GM-CSF-treated embryos was the lowest among the group. In blastocysts, GM-CSF treatment transiently increased MAPK activity. PD098059 attenuated the effect of GM-CSF on the morphological development, increase in cell number per blastocyst, down regulation of apoptosis, and upregulation of MAPK activity, suggesting that activation of MAPK cascade possibly mediated the embryotropic effect of GM-CSF. Conclusion: This result suggested that GM-CSF potentiated the development of preimplantation mouse embryos by activation of MAPK.
오명희,손진,정규회,Ohh, Myung-Hee,Sone, Jin,Chung, Kyu-Hoi 한국연초학회 2001 한국연초학회지 Vol.23 No.2
Insecticidal effect or sulfuryl fluoride ($SO_2F_2$) to cigarette beetle, Lasiodema serricorne (F.), was studied in two different containers, one was 1m$^3$(without cardboard block) and the other was 0.5m$^3$(with cardboard block). Adults and larvae were transferred into a small metal can before placed in the containers. Each can was held for 8, 24, 48, 72, and 96 hours after $SO_2F_2$(10, 20, 30, and 40 g/$\ell$) treatment. All adults were killed in an 1m$^3$container. Larval mortality was 99 to 100% when the dosage of SO$_2$F$_2$was 10 and 20 g/$\ell$. Cardboard was attached between two 0.5m$^3$ containers ; one was fumigation area and the other was insect area. SO$_2$F$_2$ penetrated cardboard within 24 hours in most trials. $SO_2F_2$could affect all adults and larvae regardless of their susceptibility. All adults were killed at 8 hours in a 0.5m$^3$ container. But low mortality of larvae was recorded at 8 hours when small dosage was treated (30% in 10 g/$\ell$, 87.2% in 20 g/$\ell$). The mortality was increased as SO$_2$F$_2$ dosage increased. Six different metals (stainless, copper, brass, aluminum, iron, and zinc) were also tested to study metal corrosion and discoloration. No corrosion and discoloration was observed in most metals treated with $SO_2F_2$.
Young Il Lee(李榮日),Kyu Hoi Chung(鄭圭會),Shin Han Kwon(權臣漢) 한국육종학회 1985 한국육종학회지 Vol.17 No.1
To evaluate the resistance to soybean pod borer, Grapholitha glycinivorella Matsumura, in soybean germplasm collection by means of conventional screening method, the resistant 30 lines and susceptible 31 lines selected preliminry among 1440 soybean germplasm were planted in different planting time with replications. Damages of soybean pod borer between resistant and susceptible group were significantly different and the damages of the lines planted on May 27 were severe than those lines planted on June 17. The pod borer damages seemed to be related with seed weight, and the most of resistant lines beared small seeds, but the susceptible lines tend to have large seeds. Grading of damage in evaluation of seed basis was recommendable for selecting resistant lines to soybean pod borer.
RAPD를 이용한 한국산 줄장지뱀(Reptilia: Squamata)의 종내 다양성에 관한 연구
장민호 ( Min Ho Chang ),송재영 ( Jae Young Song ),정규회 ( Kyu Hoi Chung ) 한국환경생물학회 2004 환경생물 : 환경생물학회지 Vol.22 No.2
한반도 전지역에 줄장지뱀이 서식하고 있지만,이 종에 대한 연구는 거의 전무한 상태이다. 한반도의 5지역(경기도, 충청북도, 제주도, 전라남도, 경상남도)의 한국산 줄장지뱀에 대해 RAPD method를 통한 종내 유전적 차이를 비교 하였다. 총 28개의 primer를 사용하였고, 그 중 유의성이 있는 17개의 Primer에 대한 결과를 Nei`s(1972) genetic distance를 통해 유전적 거리와 UPGMA 방법을 통한 phenogram을 구하였다. 그 결과 총 68개의 밴드를 확인 했고,이 중 87%인 59개의 polymorphism이 확인 되었다. 이를 통한 phonogram에서는 GG1, GG2, CB1, CB2, JN이 Group 1을 이루었으며,JJ1, JJ2, GN이 Group2를 이루었다. 줄장지뱀의 경우 지리적 격리를 통한 집단간의 종내 변이가 발생한 것으로 추정된다. 특히, 경상남도 지방의 유전적 독립성이 두드러지는데, 이는 다른 분류군인 어류나 양서류에서도 나타나고 있다. 따라서 이들 집단에 대한 형태적, 생태적 그리고 다른 유전적 분석 등을 통한 추가적인 연구가 필요할 것이라고 판단된다. The lacertid lizard Takydromus wolteri widely distributed in South Korea, but intraspecific diversity of this species was not almost studied. T. wolteri represented by 8 specimens collected from 5 localities were analyzed by the Randomly Amplified Polymorphic DNA (RAPD) method with 28 random decamer primers and the phenogram constructed by using the Unweighted Pair Group Method with Arithmetic Mean (UPGMA) method based on RAPD data. The populations of T. wolteri using in this paper were collected from Gyeonggi-do, Chungcheongbuk-do, Jeju-do, Jeollanam-do and Gyeongsangnam-do in South Korea. Among 68 different bands detected in RAPD analysis, 59 bands (87%) showed polymorphism. The phenogram showed that the populations of T. wolteri were grouped into two. These results suggest that T. wolteri were supported intraspecific diversity.
송재영 ( Jae Young Song ),장민호 ( Min Ho Chang ),정규회 ( Kyu Hoi Chung ) 한국환경생물학회 2005 환경생물 : 환경생물학회지 Vol.23 No.2
한국산 산개구리류 3종의 분류학적 형질을 명확히 하기 위하여 형태학적, 계량형태학적 방법을 이용하여 한국산 산개구리류 3종(북방산개구리, 계곡산개구리. 아무르산개구리)과 일본산 산개구리류 1종 (R. tsuchimensis)을 비교분석하였다. 형태학적 비교 분석결과, 산개구리류는 손가락 끝과 발가락 끝의 모양, 아래턱에 형성된 반은의 발달유무, 윗입술선의 유무, 물갈퀴의 발달 정도, 서구개치열의 형태 등이 차이가 있는 것으로 확인되었으며, 계량형태학적 분석을 통하여 고막의 직경과 발길이의 차이를 확인할 수 있었다. 따라서, 이러한 형태학적, 그리고 계량형태학적 특징은 한국산 산개구리류를 동정하는데 매우 유용한 형질이라고 생각된다. To clarify the key characteristics of Korean brown frogs (R. dybowskii, R. huanrenensis and R. amurensis coreana), this study employed a morphological and morphometrical comparisons among brown frogs found in Korea and Japanese endemic species, R. tsushimensis. As a result, it was considered that the key characteristics were the shape of the finger and toe tips, the markings of the lower jaw, the presence or absence of an upper lip line, the development of toe webbing, and the shape of the vomerine teeth series by morphological comparisons of brown frogs. Also, there were significant differences in tympanum diameters and foot lengths of the four species of brown frogs. Therefore, a comparison between morphological and morphometric characters is an effective way to clarify the identification of brown frogs.