금속 이온이 방사선 조사에 의한 DNA의 과산화에 미치는 영향

전정동,서현숙,한창렬 인제대학교 1986 仁濟醫學 Vol.7 No.2

방사선 조사에 의한 생체세포내 거대분자의 손상은 DNA의 구조 변경으로 인한 유전변이로 대표될 수 있다. 이에 저자들은 방사선 조사에 의한 DNA의 손상에 관여하는 반응성 산소 대사물을 규명하고 이들과의 상호 반응에 의하여 영향을 미치는 금속이온들에 대하여 연구 분석하였다. Damage of DNA caused by x-ray irradiation may be attributable to their direct or indirect actions, and these effects may be associated with oxidative reactions of reactive oxygen species. To identify reactive oxygen species which cause damage of DNA, effects of various scavengers for O2, H2O2, OHㆍand 1O2 on damaging effects of radiation were investigated. Since interaction of reactive oxygen species with metal ions was reported, influence of metal ions of damage of DNA caused by x-ray irradiation was observed. Followings are resulted. 1.Production of malondialdehyde like substance from MDA by x-ray irradiation was enhanced with increasing radiation does and this effect was also observed with xanthine-xanthine oxidase system. 2.Damage of DNA x-ray irradiation was effectively inhibited by superoxide dismutase, a scavenger for O2, catalase, a scavenger for H2O2, mannitol, formate and dimethylsulfoxide which are scavenger for OH · and histidine and 2,3-dimethylaminobenzoic acid which are quencher for 1O2. 3.Among the metal ions tested, Cu++ have been suggested to catalyze the production of OH · 1O2 from less reactive species such as O2 and H2O2, and to enhance damaging effects for cellular macromolecules, including DNA by x-ray irradiation.

식도위접합부의 방사선학적 고찰

전정동 대한영상의학회 1982 대한영상의학회지 Vol.18 No.4

In evaluating the esophage-gastric junction, many difficulties are well known in a single contrast study, and it may often be resolved with double contrast technique. By double contrast technique, the special en face view for the esophage-gastric junction was obtained in 298 cases at Inje Medical College Paik Hospital from January, 1981 to June, 1981. Five basic patterns were recognized and classified from type I to V. Their incidences were obtained. - type I; 18%, type II; 50%, type III; 23%, type IV; 7%, type V; 2%. The most common position of patients is left lateral, and zero degree is the most common at elevation of fluoroscopic table. Type III is the most common type of the esophage-gastric junction in inspiration, and type I in expiration. Any significant deviations from these normal geometry of the esophage-gastric junction as displayed by double contrast study, indicate an abnormality. More special attentions are required to evaluate a minute pathologic condition of 3th esophago-gastric junction.

방사선조사에 의한 조직손상에 있어서 반응성산소대사물, 금속이온 및 환원제의 역할

전정동,이정수,박수성 중앙대학교 의과대학 의과학연구소 1986 中央醫大誌 Vol.11 No.3

It has been reported that x-ray irradiation causes chemical and structural changes of cellular macromolecules and have been shown to induced loss of function of protein, inactivation of enzyme activities and mutagenecity due to structural changes of DNA. Damages of cellular macromolecules caused by x-ray irradiation may be attributed to their direct or indirect actions, and these effects may be associated with oxidative reactions of reactive oxygen species. The potential clinical uses of superoxide dismutase and cysteamine for ameriolation of late radiation effect has been proposed. Thus, it has been suggested that substance which have quenching effects on reactive oxygen species may reduce the tissue damages caused by x-ray irradiation. In the present study, generation processes of reactive oxygen species released by x-ray irradiation were observed through measurement of endogenous superoxide dismutase and catalase activities. To identify reactive oxygen species which cause damages of cellular macromolecules, effects of various scavengers of 0_2, H_2O_2, OH and ^10_2 on actions of radiation were also investigated. Since interaction of reactive oxygen species with metal ions or reducing agents were reported, influence of metal ions and reducing agents on damages of cellular macromolecules caused by x-ray irradiation were observed. In whole body irradiated mouse, spleen Cu^++, Zn^++ superoxide dismutase and Mn^++ superoxide dismutase activities, and kidney catalase activities were increased with time for 10 days following the initial single exposure to radiation. With increasing of radiation dose, lipid peroxidations of microsomes were enhanced but sulfhydryl contents of protein were gradually reduced. Such phenomena were also observed with xanthine-xanthine oxidase system, and lipid peroxidation and loss of sulfhydryl groups were increased with incubation time or increasing concentration of xanthine oxidase. Production of malonyldialdehyde like sub_stance from DNA by x-ray irradiation was enhanced with increasing radiation doses and this effect was also observed with xanthine-xanthine oxidase system. Damages of microsomes and DNA were effectively inhibited by superoxide dismutase, a scavenger for O_2, catalase, a scavenger for H_20_2, mannitol, formate and dimethylsulfoxide which are scavenger for OHㆍ, and histidine and 2,3-dimethylaminobenzoic acid which are scavengers for ^10_2. Among the metal ions tested, Cu^++ showed the most potent catalytic action in the production of OHㆍ and significantly stimulated the damage of DNA caused by x-ray irradiation. Glutathione, cysteine, N-(2-mercaptopropionyl)-glycine, ascorbate and NADH inhibited the damage of DNA caused by x-ray irradiation with or without Cu^++. From these results obtained, it is suggested that radiation damages of cellular macromolecules including DNA may be attributed to 0_2, H_2O_2, OHㆍ, and ^10_2. Also, metal ions such as Cu^++ have been suggested to catalyse the production of OHㆍ or ^1O_2 from less reactive species such as 0_2 and H_2O_2. However, SH group containing compounds and other reducing agents may suppress generation of reactive oxygen species by x-ray irradiation.

선천성 척추 측만증의 방사선학적 고찰

전정동,한상석,은충기,한창열 인제대학교 1984 仁濟醫學 Vol.5 No.4

Congenital scoliosis is defined as a lateral curvature of the spine caused by congenital anomalies of the vertebral development. Radiologic studies have become extremely important in the evaluation and management of congenital scoliosis. Main goals of the radiologic evaluation are to establish a precise diagnosis, monitor the progression of the deformity, search for associated anomalies, and to establish the value of treatment technique. Radiologic findings of eight cases in congenital scoliosis were reviewed, and the results are as follows: 1.The most prominent type and location were hemivertebra and thoracic area, respectively. Especially 3 patients showed one or more types of anomaly and 2 patients had multiple hemivertebra. The average curved angle estimated by Cobb's method is 34.6˚, regarded as mild and moderato deformity. 2.One diasteatomyelia and two congenital heart disease were found as associated anomalies. No evidence of kidney anomaly was not found in our cases. 3.The first precise evaluation and 6 month-interval study are very important in radiologic evaluation. 4.For the precise classification of congenital scoliosis, tomography & computed tomography should be recommended, and for the more, in case of pediatric group, the latter as non-invasive method. 5.For the evaluation of associated anomalies in other organs, routine intravenous pyelography should be recommended and myelography as indicated.

슬관절조영술에서 필름 판독상 함정부분에 관한 방사선학적 고찰 : 반월상연골병변을 중심으로 In Meniscal Lesions

전정동,이문규,한창열 인제대학교 1988 仁濟醫學 Vol.9 No.4

슬관절조영술은 반월상연골병변이 의심되는 환자에서 초기 단계의 검사방법으로서 진단율에 있어서 높은 정확도를 가지고 많이 이용되고 있다. 그러나 위양성과 위음성 소견이 항상 내재되어 있어서 필름 판독상 정확도에 있어서 소견의 함정부분이 많은 것으로 알려져 있다. 이에 저자들은 슬관절조영술후 시행한 관절경검사와 반월상연골절제술 및 적출술후 소견을 슬관절조영술 소견과 비교하여 상이한 소견이 나온 경우를 모아 문헌 고찰과 함께 분석하여 슬관절조영술의 정확도를 높이고자 하였다. This study was to analyse the different findings between arthrography and arthroscopic arthrotomy and meniscectomy in 25 cases from January, 1985 to May, 1988. The results were as follows : 1.False negative findings were more prominent than false positive findings, and the most frequent site was posterior horn of lateral meniscus. 2.The majority of causes of false findings were popliteus tendon, synovium, and recesses on meniscosynovial junction as overlapped normal anatomical structures on menisci. 3.To get more accurate findings of arthrography, it requires accurate analysis of location of overlapped normal anatomical structures on menisci in knee joint and higher diagnostic rate under fluoroscopy. 4.If clinical application of high resolution computed topography, ultrasonography, and magnetic resonance imaging combined with arthrography it more frequent, the results imply that accuracy of preoperative radiologic diagnosis for meniscal lesions is improved.

중이 진주종에서 단순유양돌기 촬영의 진단 가치 면

백대일,전정동,전우기,이성우,한창열,박수성 인제대학교 1984 仁濟醫學 Vol.5 No.4

Detecting a cholesteatoma within complicated temporal bone by simple mastoid views is desirable but any single conventional projection appears to have reached the limit of its capacity. Several simple mastoid views must be combined to delineate cholesteatoma. Author reviewed various projections of simple mastoid views in 37 cases of cholesteatomas surgically proven at Paik Hospital Inje Medical College from 1981 to 1983 in order that diagnostic value of each view could be determined. The results were as fellows: 1.Detection rate of cholesteatoma with single projection only was followings: 75% with Owen view, 60% with Towne view, 59% with Law view, 44% with Stenvers view and 33% with Transorbital magnification view. 2.Detection rate of cholesteatoma with combined projections was followings: 60% with Law, Stenvers and Towne view combination, 83% with Law, Owen and Transorbital magnification view combination. 3.Cholesteatoma involved attic in 87%, antrum in 59% and middle ear in 33%, and the combined involvement(57%) was rather frequent than single involvement (43%) 4.The mastoid air cells were poorly pneumatized in cholesteatoma, ie. sclerotic pneumatization in 81% and diploic pneumatization in 14%.

副腎의 電算化 斷層撮影

서상일,전정동,전우기,유필문,한창열 인제대학교 1983 仁濟醫學 Vol.4 No.4

저자들은 CT를 이용하여 정상 봉신의 크기 모양 및 위치를 분석하여 한국인의 부신의 정상 범위를 정하였으며 CT는 재래의 방법보다도 쉽게 부신을 발견할 수 있었고 부신질환의 진단에 중요한 역할을 할 것으로 사료된다. Computed tomography is an excellent screening test far adrenal glands. Abdominal CT scans of 94 randomized patients without evidence of adrenal disease were reviewed to determine the location, size, and shape of both side normal adrenal glands. Results are as follow: 1.Adrenal glands are shorten in 85 patients. (90.4%) 2.Most of the right adrenal glands were linear in shape. (71%) 3.and most of the left adrenal glands were inverted V or Y, or triangular in shape. (79%) 4.Right adrenal glands. Mean length of 2.2cm, Width of 2.35cm, and Thickness of 0.59cm Left adrenal glands, Mean length of 2.5cm, Width of 2.09cm, and Thickness of 0.69cm. 5.Thickness of limbs was always less than 5mm.

당뇨병에 관한 폐결핵의 방사선학적 고찰

한창열,이성우,유필문,전우기,전정동 인제대학교 1985 仁濟醫學 Vol.6 No.1

저자는 당뇨병 환자 중 71명의 폐결핵 환자에 대한 흉부 사진상 폐결핵 변화를 관찰하고 문헌적 고찰하였다. We selected seventy one pulmonary tuberculous patients (6.271) among 1150 diabetic patients which were diagnosed at Paik Hospital, Inje Medical College from Jan. 1981 to Jun. 1984. It was found the results as follows. 1.Radiological extend of pulmonary tuberculosis was as follows: minimal 45.1%, moderately advanced 46.5%, far advanced 8.4%. 2.Pulmonary tuberculosis in diabetic patient was most prevalent in 50 decade and 60 decade. It tended that the more taking of age, the more having increase pulmonary tuberculosis. 3.We observed no correlation between FBS and extend of pulmonary tuberculosis on a chest roentegenogram. 4.The longer the duration of diabetes mellitus, the higher incidence of pulmonary tuberculosis. However, there was no correlation between duration of diabetes mellitus and extend of pulmonary tuberculosis on chest roentgenogram. 5.The pulmonary tuberculosis was predominantly in upper lobes in 69.1% and the lower lobe was affected in 11.2%.

횡격막 이행부위 및 횡격막하식도의 초음파 소견

이광설,황대현,김호균,심재찬,전우기,김정숙,이기재,전정동,한창열 대한영상의학회 1990 대한영상의학회지 Vol.26 No.6

During two months from May, 1989, sonographic findings of juxta-and subdiaphragmatic esophaus were obtained in 120 cases (88%) among 137 cases of normal patients. The esophageal wall is seen as three different echoic layers ; hyperechoic mucosa, hypoechoic intervening layers, hyperechoic adventitia. The esophageal image is truncated horn shape (66%) and tubular shape (34%) on longitudinal section, and oval shape (97%) on cross section. The quality of esophageal image is intimately proportionate to the degree of the development of left lobe of liver. On abdominal ultrasonogram, the normal esophageal image of juxta-and subdiaphragmatic esophagus will be used fro evaluation of the pathology of the esophagus.