탐구력 신장을 위한 기능평가에 관한 연구

전인숙 晋州敎育大學校 科學敎育硏究所 1983 科學敎育硏究 Vol.9 No.-

Role of RS-1 derivatives in homology-directed repair at the human genome ATG5 locus

전인숙,Jae-Cheon Shin,Seung Ryul Kim,Kwan Sik Park,Hyun Jung Yoo,이광열,이학교,Joong-Kook Choi 대한약학회 2020 Archives of Pharmacal Research Vol.43 No.6

Genome editing is a useful tool in basic andclinical research. Among the several approaches used ingenome editing, the CRISPR–Cas9 system using clusteredregularly interspaced short palindromic repeats (CRISPR)and CRISPR-associated protein 9 (Cas9) along with a guideRNA has been developed recently. The CRISPR/Cas9 systeminduces site-specific double-stranded DNA breaks,which result in DNA repair via non-homologous end joining(NHEJ) or homology-directed repair (HDR). However,HDR effi ciency is lower than that of NHEJ and accordinglyposes a challenge in genome modifi cation studies. Severalchemical compounds including RS-1 have been shown toenhance the HDR knock-in process by two- to six-fold in HEK 293 cells and rabbit embryos. Based on this fi nding,we developed an antibiotic resistance system to screen RS-1chemical derivatives, which may promote effi cient HDR. Inthis study, we report several chemical compounds with highknock-in effi ciency at the ATG5 gene locus, using HeLacell-based assays.

Modulation of store-operated calcium entry and nascent adhesion by p21-activated kinase 1

전인숙,김혜련,신은영,김응국,한헌석,홍진태,이학교,송기덕,최중국 생화학분자생물학회 2018 Experimental and molecular medicine Vol.50 No.-

Calcium mobilization is necessary for cell movement during embryonic development, lymphocyte synapse formation, wound healing, and cancer cell metastasis. Depletion of calcium in the lumen of the endoplasmic reticulum using inositol triphosphate (IP3) or thapsigargin (TG) is known to induce oligomerization and cytoskeleton-mediated translocation of stromal interaction molecule 1 (STIM1) to the plasma membrane, where it interacts with the calcium release-activated calcium channel Orai1 to mediate calcium influx; this process is referred to as store-operated calcium entry (SOCE). Furthermore, aberrant STIM1 or SOCE regulation is associated with cancer cell motility and metastasis. The p21-activated kinases (PAKs), which are downstream effectors of GTPases, reportedly regulate cytoskeletal organization, protrusive activity, and cell migration. Although cytoskeletal remodeling apparently contributes to calcium mobilization via SOCE, and vice versa, the mechanisms by which they regulate each other remain unclear. In this study, we aimed to characterize whether PAK1 modulates calcium mobilization and STIM1 localization. Our data demonstrate that PAK1 interacts with STIM1 in vitro and that this interaction was enhanced by treatment with a nascent adhesion inducer, such as phorbol 12,13-dibutyrate (PDBu). Under basal conditions, both proteins appeared to primarily colocalize in the cytosol, whereas treatment with PDBu induced their colocalization to vinculin-positive peripheral adhesions. Downregulation of PAK1 activity via chemical inhibitors or by PAK1 shDNA expression impaired STIM1-mediated calcium mobilization via SOCE. Based on these findings, we propose that PAK1 interacts with STIM1 to regulate calcium mobilization and the formation of cellular adhesions.

통일을 기원하며

전인숙 문예운동사 2000 문예운동 Vol.- No.68

단기 해외자원봉사 활동이 대학생의 글로벌 리더십에 미치는 효과

전인숙,김경미,황혜영 사단법인 인문사회과학기술융합학회 2017 예술인문사회융합멀티미디어논문지 Vol.7 No.6

본 연구는 세계화와 글로벌 시대에 따라 많은 대학에서 시행되고 있는 단기 해외자원봉사활동이 대학생의 글로벌 리더십에 미치는 효과를 파악하기 위해 시도된 단일군 사전·사후 비교조사 연구이다. 11박12일 간의 필리핀 단기 해외자원봉사에 참여한 대학생 46명을 대상하였다. 연구결과 국제교류 참여경험이 있는 대상자들이 인성영역에서 '명목적 참여’(평균 4.40±.40점)가 가장 높았고 통계적으로 유의한 차이가 있었다(p=.042). 단기 해외자원봉사 활동 전․후를 글로벌 리더십의 차이는 단기 해외자원 봉사활동 후 ‘호기심’,‘이원성’,‘인성’,‘통찰력’영역에서 증가를 보였고, 글로벌 리더십 전체 점수도 평균 3.32±.50점에서 평균 3.74±.38점으로 유의한 증가(p<.01)를 보임으로써 단기 해외자원봉사활동이 글로벌 리더십 향상에 효과가 있음을 확인 할 수 있었다. 본 연구를 통해 해외자원 봉사활동이 학생들의 인성과 통찰력의 능력을 증진시킴으로 향후 다양한 기관들과의 연계를 통한 해외자원봉사 프로그램이 개발되어야 할 것이다. This research is to understand the effect of global leadership of university students due to short term foreign volunteer activity. It is 46 participant with experience in foreign volunteer activity in the 12 day short term foreign volunteer activity in the Philippines. The research is a One-Group Pretest-Posttest Disign. The results out of the character field of experienced participants, nominal participation showed highest (4.40±.40) and showed statistical significance(p=.042). The experienced showed a increase in fields of character, duality and insight compared the inexperienced(p<.01). Also the total average of global leadership had a increase from Mean 3.32±.50 to Mean 3.74±.38 showing improvement of global(p<.01) leadership through short term foreign volunteer activity. Through this research, Foreign volunteer activity of University Students is showing improvement of global leadership of university students and programs that allow dominant participation of students should be developed.

고등학생의 비만도(BMI)에 따른 식습관, 영양지식과 식이섭취 태도와의 관계 연구

전인숙,황혜영 사단법인 인문사회과학기술융합학회 2019 예술인문사회융합멀티미디어논문지 Vol.9 No.9

This research is based on analysis of the relationship between the BMI, eating habits, nutritional knowledge and dietary attitude. This study was implemented under explanation of purpose and agreement of a total of 207 students attending in S high school of D city. The research results indicate that 25(12.1%) students are underweight, 117(56.5%) students are normal weight and 35(16.9%) students are overweight. General characteristics implied that high percentage of obese students were 3rd year and male seniors. Dietary habits implied that high percentage of obese students were dietary habits having snacks once in 3-4 days. Nutritional knowledge scores of obese students were lower compared to others that were underweight normal or overweight and dietary attitude resulted higher in normal and slightly overweight students than underweight of obese students. Gender of the student and frequency of having snacks showed influence in BMI. Based on these results this research will be used as reference in order to maintain a healthy condition, establish values of organized dietary habits and to learn weight control methods and help improve the diet. 본 연구는 고등학교 학생을 대상으로 BMI와 식습관, 영양지식과 식이섭취 태도와의 관계를 알아보고자 시도하였다. 본 연구는 D도시에 위치한 S고등학교 재학중인 학생을 대상으로 연구 목적을 설명하고 동의하에 실시하였으며, 총 207부를 최종분석 하였다. 연구결과 비만도는 저체중군이 25명(12,1%), 정상체중군이 117명(56.5%), 비만군이 35명(16.9%)로 나타났다. 일반적 특성에서 학년은 3학년 학생이 성별은 남학생이 비만군으로 유의하게 높게 나타났으며, 식습관에서는 간식섭취 횟수가 3~4일에 1회 섭취하는 경우가 비만군으로 유의하게 높게 나타났다. 영양지식 점수는 비만군이 저체중, 정상체중, 과체중군보다 점수가 낮게 나타났으며, 식이섭취 태도는 정상체중군과 과체중군이 저체중군과 비만군보다 긍정적인 식이섭취 태도를 하고 있는 것으로 나타났다. BMI에 영향을 미치는 요인으로는 성별과 간식섭취 횟수가 유의한 영향을 미쳤다. 연구결과를 토대로 청소년들의 건강한 신체를 유지하고, 식습관 및 체중조절 방법에 대한 가치관을 정립하고 식생활 향상에 도움이 되는 기초 자료가 될 것으로 사료된다.

가정간호이용 대상자의 가정간호 서비스 이용실태 분석 - 1, 2차 시범사업 기간을 중심으로 -

전인숙,소애영 다문화건강학회 2021 다문화건강학회지 Vol.11 No.1

Purpose: This study was analyzed the service utilization of subjects who participated in the home nursing care pilot project to improve the home nursing care service management system. Methods: The participants were 944 patients who received home nursing care services during the first and the second pilot project period. The study design was descriptive study to analyze the general characteristics, sickness characteristics, and records of the types of requests and use of home nursing care services. Results: There were many cases of home care nursing patients in the order of cesarean section, cerebral vascular disease, and cancer. The most common types of home care requests were suture removal and OP site dressing. The performance of home care was in the order of patient and caregiver education/training, dressing, removal of sutures, and breast massage. Conclusion: To activate the home nursing care service of medical institutions, it is necessary to supply more professional, efficient, and appropriate home nursing care services and establish a linkage system with other welfare services.

질문을 통한 쓰기 과제 구체화 지도 전략

전인숙 한국초등국어교육학회 2012 한국초등국어교육 Vol.48 No.-

이 연구는 초등학생 필자들이 쓰기 과제에 적절한 내용을 생성하여 필자와 독자사이에 성공적인 의사소통이 가능한 글을 쓸 수 있도록 도와주기 위한 교사의 질문을탐색하였다. 이 연구는 학생 필자의 쓰기 과정과 결과물 그리고 연구자의 쓰기 교수경험을 토대로 하였다. 쓰기 과제 구체화는 학생 필자에게 쓰기 과제로 무엇을 제시해 줄 것인가와 각구성 요소를 어떻게 정교화 할 것인가의 두 가지 측면에서 이루어진다. 이 연구는쓰기 과제를 ‘어떻게’ 구체화 하는가에 초점을 두고 있으며, 이 때 교사의 질문이 핵심적인 역할을 한다는 점에 주목하였다. 쓰기 과제를 구체화한다는 것은 필자 자신만의고유한 경험을 끌어와 글을 읽게 될 독자 및 쓰기 목적에 맞게 관련짓는다는 것을의미한다. 연구 결과에 따르면, 교사 질문은 쓸 내용을 구체화하는 것 뿐 아니라 학생 필자와교사 간에 친밀감을 형성하여 학생 필자가 자신의 경험을 쓸 내용으로 불러오는 데있어서 인지적·심리적 부담을 줄이는 역할을 한다. 이와 같은 교사 질문은 학생 필자를 쓰기 과제로 끌어들이는 질문과 쓰기 과제와 관련된 내용 생성 질문으로 대별된다. 전자에는 학생 필자와 친밀감을 형성하는 질문과 쓰기 과제에 학생 필자의 주의를 집중시키는 질문이 포함된다. 후자에는 필자 자신의 경험 끌어오기, 교사의 구두작문 시범, 초고쓰기와 관련된 질문 이 포함된다. This paper researches teacher’s questions for effective communication on the ground of elementary student writer’s producing proper contents of writing assignments. The paper was established student writer’s writing process, products, and researcher’s teaching experiences. Writing assignments specification discusses ‘what’ and ‘how’ by writing assignments. This research focuses writing assignments specification on ‘how’ specification and teacher question’s important. Writing assignments specification means that writer relates own’s peculiar experiences with writing purpose and audiences. According to research, teacher’s questions contribute not only specific contents generation but also immediacy between teacher and students. Teacher’s questions are divided into student’s attracted writing assignments and contents generation. The former contains formation of immediacy and attention of writer’s task, the latter contains visualizing of experiences, oral composition modeling and drafting.

초기 쓰기의 특징과 지도 방향

전인숙 한국초등국어교육학회 2008 한국초등국어교육 Vol.37 No.-

이 연구는 초기 글쓰기의 특징을 몇 가지 사례를 통해 살펴보고, 초기 쓰기 지도의 방향을 모색하는 것을 목적으로 한다. 이 연구에서는 초기를 만3세 이후 초등학교2학년 시기로 규정하였다. 이 시기는 표준적 글쓰기(맞춤법에 맞게 쓰기)가 완성되기 이전 단계로 창안적 글쓰기 단계와 표준적 글쓰기로의 진입 단계이다. 초기 쓰기의 특징은 문헌과 실제 사례를 통해 살펴볼 것이다. 그리고 초기 쓰기의 특징으로부터 초기 단계 쓰기에 적합한 지도 방향을 살펴보고자 한다. 초기 글쓰기에서는 띄어쓰기, 맞춤법, 구두점과 같은 형식적인 것보다 의미를 표현하는 것을 중심에 두어야 한다. 그리고 실제 이 단계에 있는 아이들은 의미를 표현하기 위한 의사소통의 도구로 글쓰기를 수행하는 모습을 보였다. 따라서 초기 글쓰기 에서는 받아쓰기나 맞춤법과 같은 기계적인 요소보다 읽기와 말하기와 통합한 쓰기,의미 표상으로서의 쓰기가 이루어져야 한다. The purpose of this study is to research the characteristics of beginning writing and the teaching direction of beginning writing. This study prescribe that the begining writing period is range from 3 years old to second grade in elementary school. This period is to enter stage of invented writing and conventional writing before to complete conventional writing. Characteristics of beginning writing are investigated from researches and examples. From these characteristics, The study introduce teaching direction of beginning writing. When children are the beginning writing, The representation of meaning are more important than the mechanics such as punctuation marks, rules of spelling and spacing words. Then actually, the period indicate the writing for communication that represent the meaning. Therefore, the beginning writing period, the teaching is focused on integrated of reading, talking and writing for representation of meaning than mechanics.

농촌 지역 주민맞춤 ‘우리마을 예쁜치매쉼터 프로그램’ 사례

전인숙 한국농촌간호학회 2023 한국농촌간호학회지 Vol.18 No.2

Purpose: This study describes how public health officials running clinic-centered around “Our Village’s Pretty Dementia Shelter” improved their ability to cope with dementia and health by implementing early dementia screening and cognitive intervention programs. Methods: This study targeted 11 hopeful seniors from 6 villages, who were residents of the area under the jurisdiction of Health Clinic B located in County A, were over 65 years of age, and had not experienced Our Village's Pretty dementia shelters. Results: The results of the Cognitive Screening Test (CIST) showed that scores improved on all evaluation items and depression decreased. Through the dementia prevention program, health improved, vitality increased through leisure activities and cultural experiences in daily life, and the quality of life improved. Aadditionally, participating with close neighbors has become an activity that can make dementia prevention activities a habit and widespread practice. Conclusion: This case demonstrate the need for continued implementation of dementia prevention and health promotion programs for rural residents. Accordingly, it is necessary to continuously operate dementia prevention programs by diversifying them and securing expertise from rural nurses.