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In vitro plant regeneration from axillary buds of Hibiscus syriacus L.
전서범,Seung-Won Kang,Wan-Soon Kim,Gung-Pyo Lee,김선형,서상규 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.2
Presently, we report a simple, reproducible and high frequency plant regeneration in Hibiscus syriacus L. using axillary buds. H. syriacus was regenerated from axillary buds directly or through a callus phase. Regenerated shoots were directly induced from young and fresh axillary buds cultured on Murashige and Skoog medium (MS) supplemented with 0.01 mg/L of the growth regulator thidiazuron (TDZ) after 2 weeks of culture. Directly induced shoots were transferred to hormone-free MS medium and root development was observed after 6 weeks. On the other hand, old and stale axillary buds were regenerated to shoots via callus induction on MS medium containing 0.01–2 mg/L TDZ after 4 weeks. A TDZ concentration of 0.01 mg/L was most effective in callus formation. Green callus was transferred to MS medium containing 0.01 mg/L α-naphthalene acetic acid (NAA) and 0.5 mg/L benzylaminopurine (BA). After 4 weeks, callus had developed into multiple shoots. Plantlets were formed from 10 week cultures of single shoots on hormone-free MS medium. Regenerated plantlets were cultured on MS medium for one month and then transferred to pots containing garden soil. Potted plants were acclimatized for one month and grown to maturity under greenhouse conditions. The present study has shown that various concentrations of plant growth regulator can be effective for in vitro plant regeneration of H. syriacus. The direct and indirect regeneration protocol presented here will be useful for understanding the manipulation and propagation of H. syriacus.
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Characterization and expression pattern of IbPRP1 and IbPRP2 stress‐related genes from sweetpotato
서상규,전서범,김지성,신지민,김종보,강승원,이궁표,김선형 한국유전학회 2010 Genes & Genomics Vol.32 No.5
Two putative stress‐related genes were isolated from sweetpotato and were designated as IbPRP1 and IbPRP2 (Ipomoea batatas proline‐rich proteins 1 and 2). The deduced amino acid aligment of IbPRP1 and IbPRP2 shows that these two genes belong to the AAI_LTSS superfamily. Proteins in this family are known to play primary roles including defending plants from pathogens and insects, lipid transport between intracellular membranes, and nutrient storage. The mRNA expression of IbPRP1 and IbPRP2 were investigated and the results demonstrate that IbPRP2 has tissue‐specific expression. Moreover, IbPRP1 and IbPRP2 may be involved in response to various stresses including drought, pathogen, and oxidative stress. In addition, when leaf disc test was performed, the IbPRP1 transgenic tobacco plants showed increase in tolerance to salt (100 mM, 200 mM, and 300 mM). Moreover, IbPRP1and IbPRP2 may have some roles of transmembrane protein in sweetpotato when checked through GFP fusion cell localization and transmembrane analysis. All of these results indicate that IbPRP1 and IbPRP2 might play an important role in plant stress responses.
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서상규,이정은,전서범,이병현,구본철,서세정,김선형 한국식물생명공학회 2009 식물생명공학회지 Vol.36 No.4
The carbon dioxide concentration of the atmosphere is projected to increase by almost 50% over the first 50 years of this century. The major cause of this increase is continued combustion of fossil fuels. As a result, the significant changes in climate that have already occurred will be amplified, in particular a global temperature increase. Renewable energy production has a central role to play in abating net CO2 emissions to a level that will arrest the development of global warming. Especially, biomass crops are becoming increasingly important as concerns grow about climate change and the need to replace carbon dioxide- producing fossil fuels with carbon-neutral renewable sources of energy. To succeed in this role, biomass crop has to grow rapidly and yield a reliable, regular harvest. A prime candidate is Miscanthus, or Asian elephant grass, a perennial species that produces over 3 metres of bamboo-like stems in a year. Miscanthus species are typically diploid or tetraploid. Hybrids between species with different ploidy levels result in the highly productive triploid hybrids, M. × giganteus. Here we will detail the Miscanthus characteristics desired of a biomass fuel crop.
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An IbEF1 from sweet potato promotes flowering in transgenic tobacco
김지성,서상규,전병기,이영우,전서범,최정우,김종보,김선태,김선형 한국유전학회 2011 Genes & Genomics Vol.33 No.4
A novel gene, denoted IbEF1isolated from sweet potato corresponded to the most abundant mRNA present in root tissue under salt stress. However, the encoded protein did not accumulate in the shoots. Transgenic tobacco plants were generated to elucidate the physiological function of IbEF1. The extent of early flowering of these plants correlated well with the level of IbEF1 expression, implicating IbEF1 as a positive regulator of flowering of tobacco plants. Over-expression of IbEF1 enhanced the AP1 gene in response to flowering time, but not floral organ development. These results demonstrate that simple manipulation of IbEF1 activity has great potential with regard to flowering time.
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최재혁(Jae-hyuk Choi),강기준(Ki-jun Kang),조민(Min Jo),이진원(Jin-won Lee),정희림(Hee-lim Jung),전서경(Seo-kyung Jun),신창범(Shin Chang-beom),전기석(Jeon ki-suk) 한국HCI학회 2014 한국HCI학회 학술대회 Vol.2014 No.2
본 연구는 스마트폰(smart phone)을 기반으로 하는 앱세서리(appcessory)의 개발 연구를 목적으로 진행되었다. 최근 모바일 시장은 소프트웨어 시장의 성장에 따라 이를 구현시켜줄 수 있는 앱세서리 시장이 떠오르고 있다. 개인 사용자를 위한 앱 시장은 매우 활성화 되어있지만 기업용 앱 시장은 아직 성장 단계이고, 뉴 어덜트(new adult)라는 새로운 소비 문화트렌드가 형성되면서 새로운 시장이 형성되었다. 이러한 새로운 트렌트를 목표로 앱세서리 기념품 제작을 통한 바이럴 마케팅을 연구 제안한다. 여행용 가방에 초점을 맞추어 블루투스(Bluetooth) 기능을 통한 위치추적, 장력 센서을 통한 무게측정, 적외선 센서를 통한 보안 기능을 탑재한 기능을 구현할 예정이다. 따라서 제품은 여행용 기념품 패키지로 Porter, Tag, Belt의 형태로 제작될 것이다. 서비스 모델은 스마트폰 제조업체에서 제작하여 호텔에 납품하고 호텔에서는 투숙객을 상대로 기념품으로 제공하는 것을 기반으로 연구의 초점을 맞추었다. 기대 효과로는 간접 마케팅을 통한 고객 유치와 브랜드 이미지 노출을 예상하고 있다. 본 컨셉은 특허 출원 중에 있으며 프로토타입으로 제작하여 사용자 경험측면에서 가능성을 검증하고 있다. This study, the smart phone-based appcessory was conducted for the purpose of the research and development. Recent changes in the mobile market, the growth of the software market that can cater to implement this appcessory emerging markets. App for Home Users enterprise app market is very active, but the market is still in the growth stage, and the New Adults formed as a new trend in the new market of consumer culture was formed. This new app aims to Trent made souvenirs appcessory propose to study through viral marketing. Focusing on luggage Bluetooth function to provide location tracking, weighing over tension sensor, through infrared sensor features the ability to implement security plans. Thus, the product is packaged in travel Porter, Tag, Belt will be made in the form of. The service model produced by the manufacturer of smartphones hotel guests against delivery and souvenirs available in the hotel that is based on a focused research. Expect an indirect effect through marketing and brand image to attract customers expect to be exposed. This concept is patent pending and made a prototype to validate the user experience to be in terms of potential.
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