Amplification of the UQCRFS1 Gene in Gastric Cancers

전경화,김수영,윤정환,Jae Hwi Song,박원상 대한위암학회 2012 Journal of gastric cancer Vol.12 No.2

Purpose: The specific aim of this study is to unravel a DNA copy number alterations, and to search for novel genes that are associated with the development of Korean gastric cancer. Materials and Methods: We investigated a DNA copy number changes in 23 gastric adenocarcinomas by array-comparative genomic hybridization and quantitative real-time polymerase chain reaction analyses. Besides, the expression of UQCRFS1, which shows amplification in array-CGH, was examined in 186 gastric cancer tissues by an immunohistochemistry, and in 9 gastric cancer cell lines, as well as 24 gastric cancer tissues by immunoblotting. Results: We found common gains at 48 different loci, and a common loss at 19 different loci. Amplification of UQCRFS1 gene at 19q12 was found in 5 (21.7%) of the 23 gastric cancers in an array-comparative genomic hybridization and DNA copy number were increased in 5 (20.0%) out of the 25 gastric cancer in quantitative real-time polymerase chain reaction. In immunohistochemistry, the overexpression of the protein was detected in 105 (56.5%) out of the 186 gastric cancer tissues. Statistically, there was no significant relationship between the overexpression of UQCRFS1 and clinicopathologic parameters (P>0.05). In parallel, the overexpression of UQCRFS1 protein was confirmed in 6 (66.7%) of the 9 gastric cancer cell lines, and 12 (50.0%) of the 24 gastric cancer tissues by immunoblotting. Conclusions: These results suggest that the overexpression of UQCRFS1 gene may contribute to the development and/or progression of gastric cancer, and further supported that mitochondrial change may serve as a potential cancer biomarker.

교육 실습의 실태 분석및 개선방향

전경화 부천대학 1990 論文集 Vol.11 No.-

세계화에 대비한 유아기 전통 교육에 대한 고찰

전경화 부천대학 1999 論文集 Vol.20 No.-

In the age of globalization which is characterized by its openness, the cultural pluralism requires an attitude that is willing to understand and accept other country's cultures. In order to prepare for the 21st century's globalization and to obtain their own identity of traditional cultures, education on tradition has to be done from the age of childhood. In this paper, we investigated the current status of our childhood education on tradition and the problems of it that has been made from the western point of view. We also suggested a desirable direction of kindergarten's education on tradition based on the investigation. The results are as follows: 1. The tradition of each country is the culture that has been inherited for a long time from their own life. It cannot be classified as "Good" or "Bad." 2. Tradition is what is alive at present, not the traditum of museum. 3. The education on tradition has to be made for national identity as well as natioal survival. 4. The childhood education on tradition has to be extended to not simply traditional play but our traditional view to the world, culture of life, and the culture of museum. 5. For the efficient education on tradition, various programs must be developed which include an education utilizing cultural museum, an education using fairy tales, and an education harnessing picture books of great men of past ages.

DNA Methylation of Multiple Genes in Gastric Cancer: Association with CpG Island Methylator Phenotype and Helicobocter pylori Infection

전경화,원용성,신은영,조현민,임명구,진형민,박우배,Jun, Kyong-Hwa,Won, Yong-Sung,Shin, Eun-Young,Cho, Hyun-Min,Im, Myoung-Goo,Chin, Hyung-Min,Park, Woo-Bae The Korean Gastric Cancer Association 2006 대한위암학회지 Vol.6 No.4

목적: 유전자 메틸화는 유전자의 서열에 영향을 주지 않으면서 유전자의 발현을 억제하고 세포분열 후 그대로 보존되는 후성적 변화이다. 위암조직과 정상위조직에서 hMLH1, p16, p14, COX-2, MGMT, E-cadherin 유전자와 MINT (MINT1, 2, 12, 25, 31)의 메틸화 상태를 검사하여 위암의 발생 과정에서의 작용과 CIMP 및 Helicobacter pylori균 감염을 포함한 임상병리학적인자와의 연관성을 알아보고자 하였다. 대상 및 방법: 위암과 정상위 신선 동결 조직 각각 36예를 대상으로 MSP (methylation-specific PCR)방법을 이용하여 메틸화 상태를 분석하였고 CIMP의 분석은 MINT1, MINT2, MINT12, MINT25, MINT31의 5개 marker를 대상으로 시행하였다. Helicobacter pylori균 감염여부는 Warthin-Starry silver 염색을 통하여 분류하였다. 결과: 위암 관련 유전자인 p14, p16, MGMT, COX-2, E-cadherin, hMLH1의 메틸화는 각각 14예(38.9%), 13예(36.1%), 8예(22.2%), 10예(27.8%), 21예(58.3%), 6예(16.7%)였다. MINT1과 MINT25의 메틸화는 위암조직에서 정상위조직에서보다 통계학적으로 유의하게 높게 관찰되었다. CIMP 양성률은 위암조직에서 44.4%로 높게 나타났으며 CIMP-H 위암은 환자의 연령과 종양크기와 연관이 있었다. CIMP 양성 위암은 p16 유전자의 메틸화와 연관이 있었고 p16 유전자의 메틸화는 조직학적으로 저분화, 미만형, 궤양형성하는 위암에서 낮게 나타났다. MINT1의 메틸화는 Helicobacter pylori균과 연관성이 있었다. 결론: 위암에서 hMLH1, p16, p14, COX-2, MGMT, E-cadherin, MINT (MINT1, 2, 12, 25, 31)의 불활성화에 DNA 메틸화가 작용함을 알 수 있었고, Helicobacter pylori균에 의한 위암발생에 MINT1의 메틸화가 연관이 있음을 알 수 있었다. Purpose: Methylation of gene regulatory elements plays an important role in gene inactivation without genetic alteration. Gastric cancer is one of the tumors that exhibit a high frequency of CpG island hypermethylation. The purpose of this study was to investigate the occurrence of CpG island hypermethylation in gastric carcinoma in relation to H. pylori infection, CIMP and clincopathologic variables. Materials and Methods: We investigated the promoter methylation Status of six genes (hMLH1, p16, p14, COX-2, MGMT, E-cadherin) and CIMP in 36 gastric carcinoma tissues as well as in nontumor tissues. CIMP status was investigated by examining the methylation status of MINT 1, 2, 12, 25 and 31. The methylation status of the promoter was examined by methylation-specific PCR (MSP) and H. pylori infection was examined by histological diagnosis after staining with Warthin-Starry silver. Results: Among the 36 gastric carcinoma tissues, DNA hypermethylation was detected in the following frequencies: 14 (38.9%) for p14, 13 (36.1%) for p16, 8 (22.2%) for MGMT, 10 (27.8%) for COX-2, 21 (58.3%) for E-cadherin, and 6 (16.7%) for hMLH1. The frequencies for MINT1 and MINT25 hypermethylation were significantly higher in tumor tissues than in nontumor tissues. 16 (44.4%) of the 36 gastric carcinoma tissues were positive for the CIMP CIMP-H tumors were associated with older patients and larger tumor size than CIMP-L tumors. We found a significant association between the presence of the CIMP and hypermethylation of p16. Hypermethylation of p16 and MINT2 were significantly different when compared by age. MINT1 gene methylation was significantly associated with H. pylori infection (P=0.004). Conclusion: Our results suggest that aberrant hypermethylation of multiple tumor related genes (hMLH1, p16, p14, COX-2, MGMT, E-cadherin, MINT1, 2, 12, 25, 31) occurs frequently in gastric carcinoma tissues. The hypermethylation of MINT1 was significantly higher in the tumor tissues and was associated with H. pylori infection.

영화 <우먼 인 골드(Woman in Gold 2015)>에 나타난 퇴폐미술

전경화 忠北大學校 人文科學硏究所 2018 人文學誌 Vol.55 No.-

본문 참고

유아교육과정의 통합적 운영 : 프로젝트 접근법에 대한 일 연구 project approach

전경화 부천대학 1996 論文集 Vol.17 No.-

In this paper, we discovered the theoretical and historical backgrounds of project approach by reviewing theories developed by Dewey, Vygotsky, Gardner. We also found the characteristics of Reggio Emilia project and the roles of the teachers. The project approach is a methodolgy of teaching and learning recently introduced by Katz and Chard. However, the method has been adopted and practiced successfully in the Reggio Emilia kindergarten in Italy after World War Ⅱ. Since Reggio Emilia kindergarten has been introduced in 1992 as one of the best 10 schools through Newsweek, the project approach evoked a great interest over the world. The characteristics of Reggio Emilia project includes (1) emergent curriculum, (2) meaningful interaction between children and teachers, (3) importance of documentation, (4) active discussions and debates between teachers, (5) the role as co-teachers, and (6) provision of various teaching materials. We can conclude that the project approach will improve the quality of the early childhood education and lead a desirable whole-child development.

幼兒의 創意性 發達에 관한 小考

田慶和 부천대학 1985 論文集 Vol.5 No.-

고령 장애인의 체력단련 프로그램 이용 경험에 관한 질적 연구

전경화,임병우 한국노년학연구회 2017 한국 노년학연구 Vol.26 No.2

부모양육행동과 청소년의 삶에 대한 만족과의 관계 - 자아탄력성과 긍정적인 인지삼제의 매개효과 -

전경화 한국청소년시설환경학회 2018 청소년시설환경 Vol.16 No.1

전방 종격동에 전이된 갑상선 수질암

전경화,진형민,전정수,Kyong-hwa Jun,M,D,Hyung-min Chin,M,D,and Chung- soo Chun,M,D 대한갑상선-내분비외과학회 2004 The Koreran journal of Endocrine Surgery Vol.4 No.1

Medullary thyroid cancer (MTC) accounts for 5% to 10% of all thyroid cancers, and originates from the parafollicular or C-cells of the thyroid gland. More than 50% of patients present with a thyroid mass and up to 75% of these patients have locoregional lymph node metastasis at the time of diagnosis. The neuroendocrine C-cells of the thyroid gland secrete calcitonin, a relatively accurate tumor marker for MTC. Plasma basal and stimulated calcitonin measurements have been used to screen patients who are at risk of developing MTC and indispensable for the detection of residual MTC after initial surgical treatment. The overall survival rate of patients with MTC is intermediate to that of patients with differentiated thyroid cancer and anaplastic thyroid cancer. Postoperative radioiodine ablation therapy, chemotherapy and radiation therapy are generally ineffective. Surgical resection, therefore, remains the only definite treatment for patients with MTC. Unfortunately, residual MTC as indicated by elevated plasma basal or stimulated calcitonin levels is common even after apparent complete initial surgical resection. We present a case of metastatic MTC in the anterior mediastinum with review of the literatures. (Korean J Endocrine Surg 2003;4:55-58)