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장주교 ( Zhujiao Zhang ),오대식 ( Dae Sik Oh ),이명선 ( Myung-sun Lee ) 한국미용학회 2018 한국미용학회지 Vol.24 No.5
The purpose of this study is to compare anti-oxidant activity according to the extraction procedure of Puerariae Radix extracts, and to clarify anti-oxidant possibility and anti-oxidant efficacy. To achieve this research objective, it tested heavy metals and measured cytotoxicity, the inhibition of NO(Nitric Oxide) synthesis, the inhibitory effect of ROS production, the total polyphenol content & total flavonoids, 2,2-azino-bis (3-ethylbenzthizoline -6-sulfonic acid(ABTS) radical scavenging activity, and DPPH radical scavenging activity. The results are as follows. First of all, as a result of gauging ABTS radical scavenging activity, the Puerariae Radix ethanol extract and the Puerariae Radix water extract were gradually increased the ABTS radical scavenging activity in the concentrations of 10, 20, 40 ug/ ml. Its growth level was higher in the Puerariae Radix ethanol extract than in the Puerariae Radix water extract. Synthesizing the above findings, the Puerariae Radix ethanol extract had the higher anti-oxidative effect than the Puerariae Radix water extract, and can be known to be a safe material available for being used as a raw material of anti-oxidant, functional cosmetics and food. Given being further carried out a research of mechanism on the probability of a raw material in functional cosmetics through the anti-oxidant activity of Puerariae Radix ethanol extract, it is considered to be likely capable of being helpful for boosting the possibility of developing Puerariae Radix ethanol extract as the safest natural material that has multiple functionality in whitening and wrinkle improvement.