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임진혁,명노해,Lim, Jin-Hyeuk,Myung, Roe-Hae 한국군사과학기술학회 2009 한국군사과학기술학회지 Vol.12 No.1
In this study, the relationship between military pilot's spatial ability and unusual attitude recovery was investigate. MRT(Mental Rotation Test) was measured with spatial ability whereas recovery time and error rates were mearsured with respect to 11 unusual attitudes. Eight fighter pilots and eight rear cockpit pilots of F-4E participated in this study. The results showed that MRT response time was significantly correlated with unusual attitude recovery time. The regression equation showed that unusual attitude recovery time was linearly related to MRT response time and could be explained by MRT response time more than 66%. In conclusion, it is recommended that a training is needed to improve the mental rotation ability in a visually restricted environments during maneuvering.
α2,6-Sialyltransferase 과발현을 통한 인간형 시알산 부가 hCTLA4-Ig 생산 CHO 세포주 제작
임진혁(Jin-Hyuk Lim),차현명(Hyun-Myoung Cha),박혜진(Heajin Park),김하형(Ha Hyung Kim),김동일(Dong-Il Kim) 한국생물공학회 2017 KSBB Journal Vol.32 No.3
Sialylation is important in producing therapeutic proteins such as antibody, cytokine and fusion protein. Thus, enhancement of sialylation is usually performed in CHO cell cultures. α2,6-Sialyltransferase (ST), which plays a key role in the attachment of α2,6-sialic acid, is present in human cells but not in Chinese hamster ovary (CHO) cells. Overexpression of α2,6-ST can be used for enhancing the degree of sialylation and achieving human-like glycosylation. In this study, we constructed CHO cells producing human cytotoxic T-lymphocyte antigen4-immunoglobulin (hCTLA4-Ig) as well as α2,6- ST. Transfected CHO cells were selected using G418 and stable cell line was established. Profiles of viable cell density and hCTLA4-Ig titer in an overexpressed cell line were similar to those of a wild-type cell line. It was confirmed that the total amount of sialic acid was increased and α2,6-sialic acid was attached to the terminal residues of N-glycan of hCTLA4-Ig by ESI-LC-MS. Compared to 100% of α2,3-sialic acid in wild type cells, 70.9% of total sialylated N-glycans were composed of α2,6-sialic acid in transfected cells. In conclusion, overexpression of α2,6-ST in CHO cells led to the increase of both the amount of total sialylated N-glycan and the content of α2,6- sialic acid, which is more resemble to human-like structure of glycosylation.
CHO 세포에서 누에 혈림프 유래 Storage-protein 2의 세포응집 및 세포사멸 억제 효과
임진혁(Jin-Hyuk Lim),차현명(Hyun-Myoung Cha),김지훈(Z-Hun Kim),최용수(Yong-Soo Choi),김동일(Dong-Il Kim) 한국생물공학회 2016 KSBB Journal Vol.31 No.1
Chinese hamster ovary (CHO) cells have been widely used for production of various recombinant proteins such as cytokines and monoclonal antibodies. The cell aggregation and cell death in CHO cell culture directly affect cell viability, and productivity and quality of products. In this study, we investigated preventing effects of storage-protein 2 (SP2) derived from silkworm hemolymph on cell aggregation and cell death in CHO cell culture producing albumin-erythropoietin (Alb-EPO). The viable cell density in the culture supplemented with 2 mg/mL SP2 was 1.71-fold higher than that in control culture. Increased titer of Alb-EPO was also found in the culture with SP2. Morphology of CHO cells in SP2 supplemented cultures did not differ from that of control. In addition, the cell aggregation rate of the SP2 cultures was reduced 20% compared to the control. Finally, we confirmed that the apoptosis was strongly suppressed by addition of SP2 in the cultures. These results clearly demonstrate that SP2 can be served as an effective supplement for enhancing titer of Alb-EPO via reducing cell aggregation and cell death.
CHO 세포의 2단계 배양을 통한 Albumin-erythropoietin의 시알산 증대
임진혁(Jin-Hyuk Lim),신수아(Soo-Ah Shin),차현명(Hyun-Myoung Cha),김동일(Dong-Il Kim) 한국생물공학회 2016 KSBB Journal Vol.31 No.4
In glycoprotein, Terminal sialic acid residues of Nlinked glycan are imperative things because they prevent the recognition from asialoglycoprotein-receptor that affect the half-life of glycoproteins. So establishment of culture process for enhancing sialic acid is important to maximize sialic acid contents of glycoprotein. In this study, we investigated effects of biphasic culture of Chinese hamster ovary (CHO) cells producing albumin-erythropoietin to increase sialylation. Biphasic cultures were performed with shift of CO₂ concentrations and temperatures at day 5 when viable cell density was decreased and sialidase was started to be released by cell lysis. The examined temperature set points were 33, 35 and 37℃ respectively and the CO₂ concentration was 1, 5, 10 and 15%. We confirmed that sialidase activity was the lowest in biphasic culture that was shifted from normal culture condition to 1% of CO₂ and 33℃ on day 5. However, the temperature and concentration of CO₂ have little effect on activity of α2,3-sialyltransferase. Also, sialic acid contents were enhanced 1.13-fold higher than that in control culture. In conclusion, Biphasic cultivation in CHO cells led to inhibition of sialidase activity and increases of sialylated glycan.
임진혁(Jinhyuk Lim),정화영(Hwa Young Jung),정훈기(Hun-Gi Jung),지호일(Ho-Il Ji),이종호(Jong-Ho Lee) 한국세라믹학회 2018 세라미스트 Vol.21 No.4
The optimal fabrication conditions for Gd 0.1 Ce 0.9 O 2-δ (GDC) buffer layer and La 0.6 Sr 0.4 Co 0.2 Fe 0.8 O 3-δ (LSCF) cathode on 1mol% CeO 2 –10mol% Sc 2 O 3 stabilized ZrO 2 (CeScSZ) electrolyte were investigated for application of IT-SOFCs. GDC buffer layer was used in order to prevent undesired chemical reactions between LSCF and CeScSZ. These experiments were carried out with 5 x 5cm 2 anode supported unit cells to investigate the tendencies of electrochemical performance, Microstructure development and interface reaction between LSCF/GDC/CeScSZ along with the variations of GDC buffer layer thickness, sintering temperatures of GDC and LSCF were checked, respectively. Electrochemical performance was analyzed by DC current-voltage measurement and AC impedance spectroscopy. Microstructure and interface reaction were investigated by scanning electron microscopy (SEM) and energy dispersive spectroscopy (EDS). Although the interfacial reaction between these materials could not be perfectly inhibited, We found that the cell, in which 6μm GDC interlayer sintered at 1200℃ and LSCF sintered at 1000℃ were applied, showed good interfacial adhesions and effective suppression of Sr, thereby resulting in fairly good performance with power density of 0.71W/cm 2 at 800℃ and 0.7V.