RT-PCR법을 이용한 백합 바이러스 LSV, LMoV, CMV의 검출

임지현,배은혜,이용진,박성한,이규준,김새로미,정용태,Lim, Ji-Hyun,Bae, Eun-Hye,Lee, Yong-Jin,Park, Sung-Han,Lee, Kyu-Jun,Kim, Sae-Ro-Mi,Jung, Yong-Tae 한국미생물학회 2009 미생물학회지 Vol.45 No.3

2008~2009년에 강원, 충남, 제주 지역의 백합 재배 농가에서 바이러스 감염 증상을 보이는 백합의 잎과 구근을 채취하였으며 RT-PCR 방법으로 Lily mottle virus (LMoV), Lily symptomless virus (LSV), Cucumber mosaic virus (CMV) 등 3 종류의 바이러스를 검출 하였다. LSV 12주, LMoV 20주, CMV 1주가 검출 되었으며 LSV에 감염된 12개의 식물체 중 7개에서는 LMoV도 검출되어 복합 감염된 것을 확인하였다. LMoV와 LSV에 의한 복합 감염은 엽맥투명화, 잎말림, 반점, 모자이크, 황화 줄무늬 등 단독 감염보다 심각한 병징을 나타내었으며 좋지 않은 환경에서 저장된 구근에서도 복합 감염이 관찰되었다. 채집된 식물체는 LMoV 감염이 가장 많았으며 Lily virus X(LVX)에 의한 감염은 검출되지 않았다. 7개 분리주의(LMoV 4주, LSV 2주, CMV 1주) 외피 단백질 유전자를 증폭한 후 염기서열을 분석하여 국내에서 발표된 백합 바이러스 염기서열(LSV:AJ516059, CMV: AJ296154)과 비교 하였으며 LMoV는 국내에서 처음으로 염기서열을 결정하여 기존에 보고된 염기서열(AJ564636)과 비교하였다. 분리주는 기존에 보고된 바이러스와 95~99%의 뉴클레오티드 염기서열 유사성을 보였으며, 이들 분리주의 분자 생물학적 특성을 밝히고 신속하고 정확한 바이러스 진단을 위해서는 전체 염기 서열 분석이 필요한 것으로 판단되었다. Leaf samples and bulbs showing characteristic symptoms of virus infection were collected from Gang-won, Chung-nam, and Jeju Province of Korea in 2008-2009. Three viruses, Lily symptomless virus (LSV), Lily mottle virus (LMoV), and Cucumber mosaic virus (CMV) were detected by RT-PCR. Virus-infected plant samples were identified; 12 plants with LSV, 20 plants with LMoV, and 1 plant with CMV. Of the twelve LSV infected samples, seven samples were found to be mix-infected with LMoV and LSV. Symptoms of LMoV and LSV mixed infection were fairly severe, like as vein clearing, leaf curling, leaf mottling, leaf mosaic, and yellow streaking. Mixed infection with LMoV and LSV was also found in lily bulbs which have been stored under unfavorable environmental conditions. LMoV predominated in our tests, whereas spread of Lilyvirus X (LVX) was not found. The nucleotide sequences of coat protein (CP) region of seven isolates (4 LMoV, 2 LSV, and 1 CMV) were compared with the corresponding regions of LMoV (AJ564636), LSV (AJ516059) and CMV(AJ296154). The nucleotide sequence homologies between reference viruses and seven isolates were 95-99%. Complete sequencing of seven isolates is necessary to obtain more information on the molecular characteristics of these viruses as well as to increase sensitivity and rapidity of viral detection.

Substance P 가 만성 아토피피부염 환자의 말초 혈액 단핵세포로부터 interleukin - 12 와 interferon - gamma 의 생성에 미치는 영향

임지현(Ji Hyun Lim),강훈(Hoon Kang),박영민(Young Min Park),조상현(Sang Hyun Cho),이승연(Seung Yeon Lee) 대한천식알레르기학회 2001 천식 및 알레르기 Vol.21 No.4

Background:In atopic patients, allergen-specific T cells express the Th2 phenotype. It is known that interleukin-12 (IL-12) is a major cytokine for the induction of Th1 responses and that a reduced release of IL-12 in atopic dermatitis patient is closely related to reduced production of interferon- gamma(INF-γ). Recently, it has been reported that substance P (SP) positive nerve fibers express increased IL-12 and that elevated levels of this neuropeptide were seen in AD patients. Objective : This study was designed to examine the effects of SP and its fragment on the production of IL-12 and INF-γ from peripheral blood mononuclear cells (PBMCs) of chronic severe atopic dermatitis (AD) patients, and moreover to establish neuro-immunomodulatory me- chanisms in chronic severe AD patients. Materials and Method : The PBMCs from fifteen chronic severe AD patients and non-atopic individuals were stimulated with Staphylococcus aureus Cowan I strain. After stimulating the PBMCs, the effects of SP and SP fragments (SP1-4 and SP4-11) on the production of IL-12 and INF-γ were analysed by enzyme-linked immunosorbent assay. Results : The levels of IL-12 and INF-γ were significantly decreased in un-stimulated AD patients than in non-atopic controls. In chronic severe AD patients, SP and SP4-11 significantly increased the production of both IL-12 and INF-γ compared to non-atopic controls. The enhancing effect of SP and SP4-11 was most significant at the concentrations of 10-8M and 10-6M. When the spantide, an SP antagonist, was added to SP and SP4-11 stimulated PBMCs, the productions of IL-12 and INF-γ were downregulated in chronic severe AD patients but not in non-atopic controls. In contrast, SP1-4 fragment did not show any specific cytokine production.

스마트 IoT 신호등 구현과 신호체계 시스템

임지현(Lim Ji Hyun),김수혁(Kim Soo Hyuk),서상진(Seo Sang Jin),양소희(Yang So Hee),엄원주,송영준,김건환,조유제 한국통신학회 2020 한국통신학회 학술대회논문집 Vol.2020 No.11

미용 종사자 교육 정도의 소비자 인식 여부에 따른 헤어스타일별 적정 시술 시간 만족에 관한 연구

임지현 ( Ji-hyun Lim ),김동표 ( Kim Dong Pyo ),박은준 ( Eun-jun Park ) 한국미용예술경영학회 2011 미용예술경영연구 Vol.5 No.1

The structure of industry in Korea has been changing to that of service industry. The quantitative growth of service industry raises competition among service enterprises but does not yet reach qualitative improvement. In this environment in which service industry undergoes quantitative growth, qualitative enhancement of beauty industry employees who can provide customer satisfaction and customer impression services is recognized as the crucial core factor in the development of beauty care companies, and in that beauty industry, too. Thereupon, in order for the beauty care companies to provide information and academic data that can satisfy customer' s eye level by establishing service strategies, this study divides consumers into those who recognize the educational degree of beauty industry employees and those who do not with the questionnaire method and examines the satisfaction with the operation time by hairstyle according to the recognition on the educational degree of the employees. As a result, about the sex ratio of the study subjects, females were more than males. And the jobs included specialized jobs, office jobs, students, independent businessmen, and salespersons in order. Their monthly income was mostly from 2 million to 3 million won, and those with high income over 4 million won were quite rare. Among the subjects, the married were more than the unmarried. Regarding the level of education, most of them were attending or graduating from college or attending graduate school or higher, and those graduating from high school or lower were quite rare. It is considered that this is correlated with the social trend of high-level education. Regarding the consumer recognition on the educational degree of beauty industry employees, the recognition group (that recognizes the educational degree of beauty industry employees) was more than the non-recognition group(that does not recognize the educational degree of beauty industry employees). About the satisfaction with the operational time by hairstyle according to the recognition group and non -recognition group, the recognition group was more than the non -recognition group. With regard to the satisfaction with the optimal operation time by hairstyle between the recognition group and non-recognition group, the male permanent style showed statistica11y significant difference in shadow perm and the non-recognition group did so separately in shadow perm and shaggy perm. And the female permanent style indicated significant difference in the hot perm style’ s magic, setting, and digital, and the general perm style’ s texture, baby, and roll straight. Socially, they think much theoretical education is needed for permanent styles. Male' s cut-style, up-style, dry-style, and dye-style did not indicate significant difference. The result is inferred to be drawn from misinterpretation that both the recognition group and the non-recognition group related it with sensual expression rather than theoretical education, so it is thought that this does not affect the operational time. In conc1usion, consumers do not spare their investment to manage their personal value and image once they have security and influence socia11y. Thus, it is deemed that if beauty industry employees who have completed proper education right for their consumer' s value and ability enhance their value by delivering their value and capacity to their consumers properly, this wi11 lead to the increment of sales and the increase of faithful costumers as we11.

논문 : TV홈쇼핑 배경음악이 소비자의 구매의사에 미치는 영향

임지현(주저자) ( Ji Hyun Lim ),박승호(교신저자) ( Seung Ho Park ) 디자인융복합학회 2015 디자인융복합연구 Vol.14 No.4

TV홈쇼핑 시장은 최근 성숙기에 접어들며 동종업체간 경쟁이 더욱 치열해지고 있는 상황이다. 소비자의 구매를 이끌어 내는 전략으로 본 연구에서는 배경음악의 장르와 템포에 따른 소비자의 구매의사를 상품 유형 별로 나누어 분석하였다. 이를 위해 TV홈쇼핑과 배경음악에 대한 문헌연구를 진행하였고, 상품 유형 별로 실제 TV홈쇼핑 방송사에서 선곡하고 있는 배경음악의 장르와 템포를 파악하였다. 마지막으로 설문조사를 통해 스포츠웨어, 생활가전, 신선/가공식품 세 가지 상품 유형 별로 구매의사가 높은 배경음악의 장르와 템포를 분석하였다. 본 연구는 TV홈쇼핑 분야에서 배경음악과 관련한 연구가 미진한 상황에서 홈쇼핑 배경음악이 소비자 구매의사에 미치는 영향을 조사하였다. 또한 상품의 유형과 음악의 변수(장르, 템포)를 세분화하여 구매의사를 고찰하였다는 점에서 의의가 있다. Recently, as TV home shopping has entered into the stage of stability, competition between the same lines of business has become more intense. Thus, this research goals have been determined in order to analyze the purchase intent of customers related with the genre and tempo of background music of TV home shopping by different types of products. In order to achieve the research goals, thorough research on related literatures have been conducted and found out about the background music. Furthermore, the characteristics of background music of actual TV home shopping broadcasting have been analyzed, focusing on the genre and tempo of the music. In addition, a survey has been conducted in order to verify what kind of music genre and tempo of the background music has an influence on the highest purchase intent of the chosen three products(sportswear, household appliances, fresh and processed foods). This study investigated the influence of background music of TV home shopping in relation to the purchase intent of customers. Also, this study has significance in the sense that it analyzed the causal relationship between the background music of home shopping and reactions of customers by categorizing products in details and using the music genre and tempo as the independent variables that affect the purchase intent of customers.

미용실의 불만족 서비스가 불만족 처리 후 고객만족에 미치는 영향

임지현 ( Ji-hyun Lim ),장미여 ( Jmi-yeo Jang ),김성남 ( Soung-nam Kim ),박은준 ( Eun-jun Park ) 한국미용예술경영학회 2013 미용예술경영연구 Vol.7 No.3

Along with the improvement of the standards of living or the changes of life patterns, service industry has been developed, and also beauty care service industry has been constantly developed and increased. With this structural condition, in beauty care service industry, most of the managers are not only focused on technical training but also activating the best customer services provided to their customers. Here, this paper aims to examine the effect of settling dissatisfying services on customer satisfaction with the subjects of both male and female consumers using beauty care services. According to the result of regression analysis to examine the effect of dissatisfying services at beauty shops on customer satisfaction after the settlement, there exists negative correlation because as dissatisfying services are more, customer satisfaction decreases more after the settlement. According to the study result, even if the males and females using beauty care service industry are provided with the services to settle the dissatisfying factors, their satisfaction with the provision of the services is low. This implies that it is needed to make efforts to analyze and figure out the services’ dissatisfying factors closely in order to let the customers recognize the process of settling the dissatisfying factors and the result after that accurately and enhance the management for the customers using beauty care service industry.

미용실 내부 이미지와 선호도가 고객만족도와 재방문의도에 미치는 영향

임지현 ( Ji-hyun Lim ),최부경 ( Bu-koung Choi ),배은미 ( Eun-mi Bae ),팽형연 ( Peng-xing Juan ),강재준 ( Jae-jun Kang ),한연옥 ( Yeon-ok Han ),권소희 ( So-hee Kwon ),최은경 ( Eun-kyung Choi ),박은준 ( Eun-jun Park† ) 한국미용예술경영학회 2014 미용예술경영연구 Vol.8 No.1

Amidst the fiercer business environment among corporations along with the expanding economy, corporations have focused on enhancing a strategic corporate image and reliability. It is the image of a store that can best demonstrate such a corporate image in connection with the characteristics of a service industry; therefore, this study is to review how the interior image of a beauty salon affects customer preference and satisfaction and their intention to revisit. As a result of discovering the effect of the interior image of a beauty salon on customer satisfaction and intention to revisit, the better the interior image of a beauty salon, the higher the preference, which was a positive relationship. Also, the better the interior image of a beauty salon, the more the intention to revisit, which was also a positive relationship. That is, in accordance with the interior design, subsidiary facilities, and service tools in the service industry, customers form satisfaction from the skills of the service industry and service along with a favorable impression. Meanwhile, this was discovered to result in the continued use of the service industry and positive referrals. In considering the above results, male and female customers who use a beauty salon were discovered to be affected by the subsidiary facilities and the latest service tools of a beauty salon based on the skills and service. From this, if service managers cultivate human resources equipped with skills and services that can be purchased by customers and are prepared with the subsidiary facilities of the user-friendly service industry, the beauty service industry is expected to advance into a futuristic industry prepared with a system.

유두상 에크린 한선종 4예

임지현 ( Ji Hyun Lim ),오신택 ( Shin Taek Oh ),김형옥 ( Hyung Ok Kim ),김정원 ( Chung Won Kim ),박영민 ( Young Min Park ),송계용 ( Key Young Song ) 대한피부과학회 2003 대한피부과학회지 Vol.41 No.6

Papillary eccrine adenoma is a rare cutaneous tumor which occurs most commonly on the distal portion of extremities as a small solitary nodule. Histopathologically, the tumor consists of multiple dilated or branching tubular structures that are lined by two or more layers of epithelial cells and shows papillary projection into the lumina. Positive staining with S-100 protein, CEA, EMA and keratin in papillary eccrine adenoma is consistent with differentiation toward the secretory epithelium of eccrine sweat gland. Herein we report four cases of papillary eccirine adenoma and review the literature. (Korean J Dermatol 2003;41(6) : 761~765)

공감하고 실천하는 민주시민을 기르는 역사수업 - ‘정서적 감정이입(affective empathy)’을 적용한 수업 방안 모색 -

임지현 ( Lim Ji-hyun ) 역사교육학회 2021 역사교육논집 Vol.76 No.-

Recently, the newly perceived historical consciousness is related to the students' proper awareness of the historical facts of the past and their interest in the historical issues that have led to the present, and to foster the power to make the better future history on their own. Also, it is to have a citizen's consciousness to make a right decision on a given problem and to practice it at the same time, and to cultivate such a democratic citizen's historical consciousness may be a purpose of history education. involves students' interest in the historical issues that have led to the present, while fully aware of the historical facts of the past, and furthermore, I will develop the power to make the better future history on their own. Meanwhile, Barton and Levstik, who emphasized democratic citizenship education in that history education should contribute to promoting common good and participatory democracy, insisted that historical empathy is a useful principle of instruction for cultivating citizenship. In particular, criticizing the emphasis on only cognitive inquiry, they suggested that affective factors such as empathy and identification should be actively combined into historical empathy. Barton and Levstik's views have been developed by researchers at local and abroad into the discussion of the re-conceptualization to properly combine historical empathy with the cognitive inquiry processes and affective elements. It is clear that these discussions are possible logic when integrating concepts based on the studies of scholars and teaching strategies of teachers. In addition to the discussion of re-conceptualization of historical empathy and analysis of class cases using sympathy and emotion, this study specifically designed a teaching plan based on the hypothesis that historical empathy applying affective dimension can greatly contribute to the growth of students as democratic citizens, and tried to find the possibility. At the same time, through the class, this study tried finding that Barton and Levstik's ‘affective empathy’ called ‘caring’ embodies the driving force that students lead the knowledge and consciousness of history learned in classes to the practice of action as the core of the civic competency. In conclusion, historical empathy should be conceptualized as the principle of instruction that combines both tools of cognitive inquiry and affective elements. Especially, the application of ‘affective empathy’ called ‘caring’ is a factor that exerts more aggressive sympathy among students, and it could be used as a key teaching principle of democratic civic education in that it plays a role in promoting interest in the ‘common good’ such as human rights, peace, and coexistence.

도형 학습에서의 오류 찾기 활동의 적용 효과

임지현 ( Ji Hyun Lim ),최창우 ( Chang Woo Choi ) 한국수학교육학회 2016 初等 數學敎育 Vol.19 No.1

본 연구는 초등수학의 여러 영역 중 도형 학습에서 오류 찾기 활동을 통하여 학생들이 범하는 오류 유형이 어떤 것이 있는지를 알아보고 이러한 활동이 학생들의 수학학업 성취도와 추론 능력에 미치는 영향을 알아 보는데 있다. 연구 결과 학생들이 가장 많이 보이는 오류는 부정확한 개념과 정의에 의한 오류(55.8%)이며, 문제해결을 기피하는 오류(14.8%)를 제외하면, 시각적 자료를 부적절하게 사용하는 오류(11.4%), 문제 자료를 곡해하는 오류 (9.2%), 기술적인 오류(6.2%), 논리적으로 부적절한 추론으로 인한 오류(1.6%), 문제 자료를 불충분하다고 여기는 오류(1.0%) 순으로 나타났다. 오류 찾기 활동을 수업에 적용한 결과, 사전 및 사후 학업 성취도 검사와 추론 능력 검사에서 유의수준 5% 이내의 유의미한 차를 보였다. 이는 오류 찾기 활동이 수학 학업 성취도와 수학적 추론 능력향상에 도움을 주는 교수 방법 중 한 가지가 될 수 있음을 알 수 있었다. In this study, the case of error became the object of learning, and the investigator applied these cases to an actual class and established three study problems in order to achieve the purpose of this study. The results of analysis of students`` errors in figure based on before achievement test are shown as follows: First, the most errors occurred in the figure was the ones from deficient mastery of prerequisite concepts and definitions. Specially, the errors from deficient mastery of prerequisite concepts and definitions have the majority. it is very high ratio even if it considers an influence of an evaluation question item. so, I think it is necessary to teach concept related figure above all. Second, as the results of application ``finding errors`` to a class, there is a meaningful difference in the mathematical achievement and reasoning ability within significance level 5%. This means ``finding errors`` is one of the teaching method that it develops the mathematical achievement and reasoning ability.