3급 부정 교합자의 수직적 골격 요소에 관한 연구

임나원,김상철,국윤아 원광대학교 치의학연구소 1992 圓光齒醫學 Vol.2 No.2

The purpose of this study was to investigate the vertical skeletal pattern of Class Ⅲ malocclusion, and to identify the morphologic differences in relation to various facial patterns. 31 normal occlusions and 59 Class Ⅲ malocclusions were selected, and Class Ⅲ malocclusions were subdivided to horizontal group and vertical group by terms of facial height ratio. Cephalograms were measured and analyzed statistically for identification of the morphologic differences in groups. The results were as follows 1. FH-MP, PP-MP, and OP-MP angle in the vertical group were larger than those in the horizontal group. 2. AB-MP angle in the vertical group was smaller than that in the horizontal group. 3. AB-MP angle in skeletal CI Ⅲ malocclusion was smaller than that in normal occlusion. 4. OP-MP angle and (OP-MP)/(PP-MP) ratio in skeletal Cl Ⅲ malocclusion were larger than those in normal occlusion.

Vitamin D3와 Dexamethasone의 복합 투여가 골모세포에 미치는 영향에 관한 연구

임나원,김상철 원광대학교 치의학연구소 1996 圓光齒醫學 Vol.6 No.3

Bone is a dynamic tissue which is constantly remodelled by subsequent cycles of bone resorption and formation. Glucocorticoid and vitamin D_3 are known as regulating substances in bone metabolism. In vitro experiments using bone tissue, it was suggested that glucocorticoid inhibits bone resorption, whereas the effect of glucocorticoid on bone formation are complex- increasing or decreasing effect. The active form of vitamin D_3. 1,25-dihydroxycholecalciferol[I.25-(OH)_2D_3], has been reported to stimulate osteoblastic activities including the production of ALP, type I collagen, and osteoclacin. The purpose of this study was to evaluate the effect of admixture of vitamin D_3 and dexamethasone, one of glucocorticoids, on osteoblastic cell line(MC3T3-El). Alkaline phosphatase(ALP) and MTT assay were conducted in the cultivated cells with 1, 10, 100nM/㎖ of 1,25-(OH)_2D_3 and/or 1μM/㎖, 100μM/㎖, 10nM/㎖ dexamethasone. The observed results were as follows. 1. The activity of osteoblastic cells with 1μM/㎖ of dexamethasone was significantly increased at 1-day cultivation with comparison to control group, but was decreased afterwards. But the activity of ALP was greatest in 1μM/㎖ of dexamethasone and increased wth time lapsed. 2. The activity of osteoblastic cells with vitamin D_3 was significantly increased dose-dependently at I-day cultivation, but was significantly decreased in 100nMJ/.㎖ at 2-day cultivation, and was a little increased again at 3-day cultivation. The activity of ALP was increased in 10nM/㎖ or l11nM/㎖ at 2-day or 3-day cultivation, and was greatest in l00nM/㎖ at 3-day cultivation. 3. In case of admixture of dexamethasone and vitamin D_3, the cellular activity was decreased in any concentration of vitamin D_3 at 2-day cultivation, but was increased again at 3-day cultivation, which was greater than that in control or dexamethasone only group. The activity of ALP was decreased at 1-day cultivation, but was increased in the admixture of 10nM/㎖ or 100nM/㎖ of dexamethasone with l00nM/㎖ of vitamin D_3 at 2-day cultivation, and was again decreased at 3-day cultivation

Vitamin D₃와 Dexamethasone의 복합 투여가 골모세포에 미치는 영향에 관한 연구

임나원(Na-Won Lim),김상철(Sang-Cheol Kim) 대한치과교정학회 1999 대한치과교정학회지 Vol.29 No.3

치아 이동시 골개조에 관여하는 골모세포의 활성을 알아보기 위해 골조직 대사 물질인 vitamin D₃를 1, 10, 100nM/ml 농도로, dexamethasone을 10, 100nM/ml, 1μ Mml 농도로 단독 또는 복합 투여하여 세포 활성 및 염기성 인산분해효소의 활성도를 분석 하여 다음과 같은 결과를 얻었다. 1. dexamethasone을 단독 투여한 경우 배양 1일째에 1μM/ml 농도에서만 대조군에 비해 유의한 세포 활성 증가를 보였으며 이후에는 전반적으로 유의한 감소를 보였다. 반면에 염 기성 인산분해효소의 활성도는 1μM/ml 의 dexamethasone일 때 가장 높았으며 배양 기간 이 길어질수록 유의한 증가를 보였다. 2. vitamin D₃ 첨가시 배양 1일째에는 세포 활성이 증가하였으나 배양 2일째 에는 100nM/ml에서 대조군과 비교해 크게 감소하여 농도의 증가에 따라 세포 활성이 크게 감소하는 경향을 보였으며 배양 3일째에는 다소 활성이 회복되었다. 염기성 인산분해효소의 활성도는 10nM/ml과 100nM/ml의 vitamin D₃에서 배양 2일째와 3일째에 대 조군에 비해 유의하게 높았는데 100nM/ml에서 배양 3일째에 가장 높았다. 3. dexamethasone과 vitamin D₃를 복합 투여한 경우 배양 2일째에는 모든 vitamin D₃ 농도에서 세포활성이 감소하였으나 3일째에는 세포 활성이 회복 되어 대조군이나 dexamethasone단독 투여 시에 비해 유의한 활성 증가를 보이는 경우가 있 었다. 염기성 인산분해효소의 활성은 배양 1일째에 감소를 보였으나 배양 2일째에 10nM/ml 나 100nM/ml 의 dexamethasone에 100nM/ml의 vitamin D₃ 복합 투여의 경우 유의한 증가를 보였고 배양 3일째에 다시 감소를 보였다. 적절한 농도의 dexamethasone과 vitamin D₃의 복합 사용으로 골모세포의 활성 및 염기성 인산분해효소를 증가시키거나 조절하는 상승효과를 얻을 수 있음을 알 수 있었다. Bone is a dynamic tissue which is constantly remodelled by subsequent cycles of bone resorption and formation. Glucocorticoid and vitamine D₃ are known as regulating substances in bone metabolism. In vitro experiments using bone tissue, it was suggested that glucoccorticoid inhibits bone resorption, whereas the effect of glucocorticoid on bone formation are complex- increasing or decreasing effect. The active form of vitamin D₃, 1,25-dihydroxycholecalciferol 〔1.25-(OH)₂D₃〕, has been reported to stimulate osteoblastic activities including the production of ALP, type Ⅰcollagen, and osteoclacin. The purpose of this study was to evaluate the effect of admixture of vitamin D₃ and dexamethasone, one of glucocorticoids, on osteblastic cell line(MC3T3-E1). Alkaline phosphatase(ALP) and MTT assay were conducted in the cultivated cells with 1, 10, 100㎚/ml of 1,25-(OH)₂D₃ and/or 10nM/ml, 100nM/ml, 1μM/ml of dexamethasone. The observed results were as follows. 1. The activity of osteoblastic cells with 1μM/ml of dexamethasone was significantly increased at 1-day cultivation with comparison to control group, but was decreased afterwards. But the activity of ALP was greatest in 1μM/ml of dexamethasone and increased with time lapsed. 2. The activity of osteoblastic cells with vitamin D₃ was significantly increased dose-dependently at 1-day cultivation, but was significantly decreased in 10nM/ml or 100nM/ml at 2-day or 3-day cultivation, and was greatest in 100nM/ml at 3-day cultivation. 3. In case of admixture of dexamethasone and vitamin D₃ at 2-day cultivation, but was increased again at 3-day cultivation, which was greater than that in control or dexamethasone only group. The activity of ALP was decreased at 1-day cultivation, but was increased in the admixture of 10nM/ml or 100nM/ml of dexamethasone with 100nM/ml of vitamin D₃ at 2-day cultivation, and was again decreased at 3-day cultivation.

성견의 실험적 치아이동시 치주조직내 DNA 합성에 관한 면역조직화학적 연구

김성진,임나원,김상철 대한치과교정학회 1996 대한치과교정학회지 Vol.26 No.4

교정력에 의한 성견의 치아이동시 발생하는 조직변화 중 치주인대 내에서의 세포활성도의 변화를 교정력의 크기와 기간에 따랄 알아 보고자 하였다. 생후 1년 6개월된 성견 6마리를 5마리의 실험군과 1마리의 대조군으로 나누었고 실험군에서 하악 좌측에는 강한 힘(250-300g)을, 하악 우측에는 약한 힘(50-75g)을 제1소구치와 제2소구치 사이에 open coil spring으로써 적용하였다. 실험군의 성견을 각각 12시간, 24시간, 3일, 1주, 2주에 Bromodeoxyuridine(BrdU)을 주입하고 희생시켰다. 하악 제1,2 소구치 부위의 조직을 채득하여 통법에 따라 파라핀 포매하였으며, H&E 염색과 항 BrdU 항체를 이용한 면역조직화학적 염색을 시행한 결과 다음과 같은 결론을 얻었다. 견인측에서의 치주인대 단절과 혈관확장은 12시간째에서 관찰되어 증가하다가 3일째 이후에는 감소되었는데 약한 힘을 준 경우보다 강한 힘을 준 경우에서 더 많았으며 압박측에서의 치주인대의 초자양변성과 파골세포 활성은 12시간째에서부터 관찰되어 3일째까지 증가하다가 7일째부터 감소하였는데 약한 힘을 준 경우보다 강한 힘을 준 경우에서 더 많이 나타났다. 대조군의 BrdU 발현은 구강상피와 치주인대의 섬유모세포에서 주로 많았고 골세포나 파골세포에서는 음성반응을 보였으며 실험군의 BrdU 발현은 압박 측보다 견인측에서 많았으며 치경부쪽보다는 치근단쪽에서 더 많았다. 약한 힘을 준 경우에서는 BrdU 발현이 1일째에 가장 많이 나타나다가 감소되었고 강한 힘을 준 경우에서는 12시간째에서 최고에 달하다가 이후에는 감소되었으나 14일째에는 실험군과 대조군 간에 큰 차이가 없었다. The purpose of this study was to investigate the changes about the cellular activity on DNA synthesis in the periodontal ligament of dog, in which experimental tooth movement was performed. A control and 5 experimental dogs, one and half year in age, were studied. Light force (50-75g) was applied by placing open-coil spring between left mandibular premolars; heavy force (250-300g), between right mandibular premolars. Experimental dogs were sacrificed after infusion of bromodeoxyuridine(BrdU), at 12hours, 1, 3, 7 and 14days after force application, respectively. And the histologic and the immunohistochemical evaluation were performed on the obtained periodontal tissue around mandibular premolars, using anti-bromodeoxyuridine antibody, which can indicate proliferating cells. The results were as follows: 1. The tearing of periodontal ligament and the vascular dilatation at tension side were observed in 12 hours, increasing until 3 days After then it decreased; Such a finding was more evident in heavy force group than in light force group. 2. The hyalinization of the periodontal ligament and the activity of osteoclast at pressure side were observed in 12 hours, increasing until 3 days. But from 7 days on, it decreased; Such a finding was more evident in heavy force group than in light force group. 3. The BrdU expression in the control group was positive, mainly in the oral epithelium and the fibroblasts in the periodontal ligament, but negative in bone cells in periodontal ligament. 4. The BrdU expression in the experimental group was more positive in tension side than in pressure side; The expression was a little more positive in the periapical area than in the cervical area of tooth. 5. The BrdU expression in light force group was the highest in 1 day, after which it decreased; In heavy force group, it was the highest in 12 hours, after which it decreased. But in 14 days, there was no difference between the experimental group and control group.