약물-고분자물질 결합체 합성연구 : 5-Fluorouracil과 사람의 혈청 Albumin 및 Poly-L-lysine 결합체 합성

이희주,신혜순,이명걸,박만기,김종국,Lee, Hee-Joo,Shin, Hae-Soon,Lee, Myung-Gull,Park, Man-Ki,Kim, Chong-Kook 대한약학회 1989 약학회지 Vol.33 No.5

The durg-macromolecule conjugates i.e. 5-fluorouracil-1-acetyl-human serum albumin(FU-AA-HSA, 8) and 5-fluorouracil-1-acetyl-poly-L-lysine(FU-AA-polylys, 9) have been synthesized and purified by sephadex G-25 gel filtration with 0.05M phosphate buffer(pH 7.5). The analyses of conjugates gave the molar ratio of FU-AA : HSA of 70-100:1 and FU-AA: poly-L-lysine of 109:1. The weight percent of FU-AA(as $FU-CH_2CO-$) in FU-AA-HSA conjugate was 16-22% and the one in FU-AA-polylys was 22.4%.

안지오텐신 변환효소(變換酵素) 억제(抑制) 작용(作用) 물질(物質)의 합성(合成)

이희주,김영숙,장영,이종란,윤혜숙,Lee, Hee-Joo,Kim, Young-Sook,Chang, Young,Lee, Jong-Ran,YunChoi, Hye-Sook 대한약학회 1984 약학회지 Vol.28 No.6

Phenylurea, phenylthiourea, benzylcarbamate and toluenesulfonate of L-alanyl-L-proline(comp. $3{\sim}6$) were synthesized and their effects against angiotensin-converting enzyme (ACE) were tested. Comp. 3 showed only mild inhibitory activities against ACE while comp. $4{\sim}6$ were inert indicating that those functional groups were not suitable for interactions with ACE. Ortho-hydroxy- or ortho-carboxy-benzamide of proline (comp. 7) and phenylalanine (comp. 8 and 9) were also tested. Of the benzamides, ortho-hydroxy function was unsuitable to exert inhibitory activities against ACE. Ortho-carboxy group of 9 seemed to have mild interactions with active site of ACE possibly because of the shorter distance between the amide and ortho-carboxyl group of the compound than the corresponding two active sites of the enzyme.

9-$\beta$-D-Arabinofuranosyladenine-5'-methoxyacetate 및 1-$\beta$-D-Arabinofuranosylcytosine-5'-methoxyacetate의 합성 및 Sarcoma 180 암에 대한 항암작용

이희주,차영애,안정욱,장일무,Lee, Hee-Joo,Cha, Young-Y.,Ahn, Jung-W.,Chang, Ill-M. 생화학분자생물학회 1984 한국생화학회지 Vol.17 No.3

9-$\beta$-D-Arabinofuranosyladenine-5'-methoxyacetate (ara-A-MA) 및 1-$\beta$-D-Arabinofuranosylcytosine-5'-methoxyacetate (ara-C-MA)를 합성하였다. 이들 물질의 항암작용을 검토한 바, sarcoma 180 암세포를 이식한 mice중 생리식염수만 공급 받은 negative 대조군은 생존일수가 $16.3{\pm}4.1$일이었고, positive 대조군인 ara-C(13.9 mg/kg/mouse)를 투여 받은 실험군은 생존일수가 $22.0{\pm}2.5$ 일이었다. ara-A-MA를 투여 받은 시험군은 $20.8{\pm}2.9$일(19.4 mg/kg/mouse) 이었고, ara-C-MA를 투여 받은 실험군은 $23.1{\pm}3.3$일(18.0 mg/kg/mouse) 및 $20.1{\pm}2.2$일(1.8 mg/kg/mouse)의 생존일수를 각각 나타내었다. 한편 이들 합성 화합물은 DNA 합성을 억제하는 작용을 나타내었는데, ara-A-MA는 Fischers media에 배양한 sarcoma 180 암세포의 DNA 생합성을 $10^{-3}\;M$ 농도에는 57% 그리고 $10^{-4}\;M$에서 50%를 억제하고, ara-C-MA는 94% 및 80%를 각각의 농도에서 억제시켰다. 이들 결과는 ara-A-MA 및 ara-C-MA가 ara-A 및 ara-C의 prodrug으로서 역할을 할 수 있는 가능성을 시사한다. 9-$\beta$-D-Arabinofuranosyladenine-5'-methoxyacetate (ara-A-MA) and 1-$\beta$-D-Arabinofuranosylcytosine-5'-methoxyacetate (ara-C-MA) were synthesized. Their antitumor activities were evaluated against sarcoma 180 tumor cells inoculated in mice. When administered intra peritoneally once daily for 6 days, average life-spans for those which received ara-A-MA, ara-C-MA, ara-C and saline (0.9%) were; $20.8{\pm}2.9$(19.4 mg/kg/mouse) and $19.1{\pm}2.5$(1.9 mg/kg/mouse) days; $23.1{\pm}3.3$(18.0 mg/kg/mouse) and $20.1{\pm}2.2$(1.8 mg/kg/mouse) days; $22.0{\pm}2.5$(13.9 mg/kg/mouse) days; and $16.3{\pm}4.1$ days, respectively. This survival study indicates that ara-A-MA and ara-C-MA exhibit antitumor activities as much potent as their parent compounds of ara-C. DNA biosynthesis in Sarcoma 180 cells in vitro were also inhibited by ara-A-MA. At the concentrations of $10^{-3}$ and $10^{-4}\;M$, ara-A-MA and ara-C-MA showed 57 and 50%, 94 and 80% inhibitions of DNA synthesis in comparison with that of negative control group, respectively.

9 - β - D - Arabinofuranosyladenine - 5 ' - methoxyacetate 및 1 - β - D - arabinofuranosylcytosine - 5 ' - methoxyacetate 의 합성 및 Sarcom 180 암에 대한 항암작용

이희주,차영애,안정욱,장일무 ( Hee Joo Lee,Young Y . Cha,Jung W . Ahn,Ill M . Chang ) 생화학분자생물학회 1984 BMB Reports Vol.17 No.3

9-β-D-Arabinofuranosyladenine-5`-methoxyacetate (ara-A-MA) and 1-β-D-arabinofuranosylcytosine-5`-methoxyacetate (ara-C-MA) were synthesized. Their antitumor activities were evaluated against sarcoma 180 tumor cells inoculated in mice. When administered intraperitoneally once daily for 6 days, average life-spans for those which received ara-A-MA, ara-C-MA, ara-C and saline (0.9%) were; 20.8±2.9(19.4 ㎎/㎏/mouse) and 19.1±2.5 (1.8 ㎎/㎏/mouse) days; 23.1±3.3(18.0 ㎎/㎏/mouse) and 20.1±2.2(1.8 ㎎/㎏/mouse) days; 22.0±2.5(13.9 ㎎/㎏/mouse) days; and 16.3±4.1 days, respectively. This survival study indicates that ara-A-MA and ara-C-MA exhibit antitumor activities as much potent as their parent compounds of ara-C. DNA biosynthesis in Sarcoma 180 cells in vitro were also inhibited by ara-A-MA. At the concentrations of 10^(-3) and 10^(-4) M, ara-A-MA and ara-C-MA showed 57 and 50%, 94 and 80% inhibitions of DNA synthesis in comparison with that of negative control group, respectively.

보육교사의 성찰역량과 전문성 인식의 관계에서 팀티칭 교사 간 소통의 매개효과

이희주 ( Lee Hee Joo ),강수경 ( Kang Su Kyoung ) 한국유아교육학회 2022 유아교육연구 Vol.42 No.2

본 연구는 보육교사의 성찰역량과 팀티칭 교사 간 소통, 전문성 인식의 관계를 알아보고, 성찰역량과 전문성 인식의 관계에서 팀티칭 교사 간 소통의 매개효과를 검증하였다. 연구대상은 서울 및 경기 지역의 어린이집에 재직한 팀티칭을 경험한 보육교사 203명이다. 자료 분석은 SPSS 26.0과 SPSS PROCESS Macro v3.5 프로그램을 사용하였으며, 각 변인 간의 상관관계를 살펴보기 위해 Pearson 상관분석을 실시하였고, 매개효과를 분석하기 위해 Hayes(2017)가 제안한 model 4 연구모형을 적용하여 자료를 분석하였다. 연구결과 첫째, 보육교사의 성찰역량과 전문성 인식의 관계. 팀티칭 교사 간 소통과 전문성 인식의 관계는 정적 상관관계가 나타났으며, 성찰역량과 팀티칭 교사 간 소통도 유의한 정적 상관관계가 나타났다. 둘째, 보육교사의 성찰역량과 전문성 인식의 관계에서 팀티칭 교사 간 소통의 매개효과는 유의한 것으로 나타났고, 하위요인 중 지식적 소통의 매개효과가 유의한 것으로 나타났다. 본 연구를 통해 보육교사의 전문성 인식 향상을 위해서 성찰역량과 팀티칭 교사 간 소통이 중요한 요인임을 규명하였고, 보육교사의 전문성 인식을 증진시키기 위한 방안을 모색하였다. This study examined the relationship among reflection competency of childcare teachers, communication between team teaching teachers, and recognition of professionalism. The mediating effect of communication between team teaching teachers was verified in the relationship between reflection competency and recognition of professionalism. This study conducted an online survey of a total of 203 childcare teachers who experienced team teaching in Seoul and Gyeonggi Province. For data, SPSS 26.0 and SPSS PROCESS Macro v3.5 programs were used. In order to examine the correlation between each variable, this study conducted analysis of person correlation and used the Bootstrapping method, to evaluate the mediating effect of each between reflection competency and recognition of professionalism of childcare teachers. The result was as follows. First, the reflection competency of childcare teachers, the communication between team teaching teachers, and the recognition of professionalism showed a high level of correlation. Second, a significant positive correlation was also revealed in the relationship between reflection competency of childcare teachers and recognition of professionalism. Third, it was found that the mediating effect of communication between team teaching teachers was statistically significant in the relationship between the child care teachers’ reflection competency and the recognition of professionalism. It was found that the mediating effect of intellectual communication between team teaching teachers was statistically significant in the relationship between reflection competency and recognition of professionalism of childcare teachers.

간호대학생의 임상수행능력, 교수-학생상호작용, 진로성숙도가 회복탄력성에 미치는 영향

이희주(Lee, Hee-Joo),이해영(Lee, Hae-young),강경자(Kang, Kyung-ja) 한국웰니스학회 2017 한국웰니스학회지 Vol.12 No.1

본 연구는 간호대학생을 대상으로 회복탄력성을 파악하고 그에 따른 임상수행능력, 교수-학생 상호작용, 진로 성숙도의 관계를 파악하기 위한 서술적 조사연구이다. 연구 대상은 4개 지역 7개 간호학과 4학년 총 411명 학생이며, PASW Statistics 23.0로 분석하였다. 간호대학생들은 성별, 건강상태, 대학생활만족도, 간호학전공만족도, 임상실습만족도, 동료와의 관계, 임상실습량에 대한 만족도, 임상실습과제량 만족도에 유의한 차이가 있었다(p<.001). 회복탄력성에 영향을 미친 변수로는 임상수행능력, 진로성숙도, 대학생활만족도, 교수-학생상호작용으로 나타났다. 추정된 회귀모형의 적합도 F통계량은 137.12(p<.001)로 유의하였고, 대학생활만족도, 교수-학생 상호작용, 진로성숙도, 임상수행능력이 회복탄력성에 대한 설명력은 62.9%이었다. 이상의 결과로 간호대학생의 회복탄력성을 증가시킬 수 있는 프로그램의 운영을 모색하고 특히 학습성과 기반의 간호교육과정 운영에 있어 간호대학생들의 임상수행능력을 키우고, 구체적 진로지도를 체계적으로 제공하며, 교수-학생 상호작용의 중요성을 바탕으로 간호대학생의 회복탄력성을 제고하는 프로그램 개발 및 적용이 지속적으로 이루어질 수 있어야 한다. This study is a descriptive survey research to investigate the resilience of nursing students and to investigate the relation between clinical performance, professor-student interaction, and career maturity. The subjects of this study were 411 senior students attending seven nursing departments of four cities. The data were analyzed using PASW Statistics 23.0. There was a significant difference in nursing students" gender, health status, university life satisfaction, nursing major satisfaction, clinical practice satisfaction, relationship with colleagues, satisfaction with clinical practice volume, and satisfaction with clinical practice assignment(p<.001). Clinical performance, career maturity, university life satisfaction, and professor-student interaction were the variables that affected resilience, and clinical performance was confirmed to be the most important variable for resilience. The regression analysis showed that the F statistic for fitness of the estimated regression model was very high at 137.12 (p<.001), and that university life satisfaction, professor-student interaction, career maturity, and clinical performance were found to affect resilience, and the explanatory power was 62.9%. From the results of this study, it is necessary to seek to operate a program that can increase the resilience of nursing students, in particular, to nurture the clinical performance of students in operating nursing curriculum based on learning outcomes, and to systematically provide specific career guidance, and to continue to make the development and implementation of programs that enhance the resilience of nursing students based on the importance of professor-student interaction.

8체질 관점으로 접근한 웰니스

이희주(Lee, Hee-Joo) 한국웰니스학회 2018 한국웰니스학회지 Vol.13 No.4

본 연구는 8체질의 근본적 장점을 파악하고, 이에 대한 생활의 전반적 접근을 통해 미래사회의 건강한 생활 영위와 질병 예방의 웰니스적 삶으로의 자리매김을 위한 접점을 찾기 위한 의견을 피력하였다. 8체질의 생활적 접근으로 물, 음식, 환경을 바라보는 관점을 이해하고, 다음과 같은 웰니스적 접근에 대해 언급하였다. 첫째, 정신적 개성 뿐 아니라 육체적 개성을 이해한 예방과 관리에 초점을 맞춘 건강관리의 자리매김이 필요하다. 그리고 우리는 자신의 체질과 인체에 대해서 더 잘 알 필요가 있다. 둘째, 병리적 상태에 따른 질병 치료가 아니라 원인이 다른 질병 치료라는 맞춤형 치료 접근이 필요하다. 셋째, 아울러 그 맞춤 치료의 접근은 정확한 체질에 따른 자연적 관리에 중심을 두어야 한다는 것이다. 넷째, 육체적 개성에 따라 에너지가 활성이 되어 도움이 되는 환경을 북돋고, 불필요한 환경을 제어해야 한다. 이러한 터득은 위와 같은 건강 행위를 기반으로 한 웰니스적 삶을 이룬다는 목적의 방향을 제시하고 인간 스스로 자생하는 힘을 믿는 중요한 기원이 될 것이다. The purpose of this study is to identify the fundamental strengths of the eight constitutions and to find out the points of contact for the healthy living of the future society and the wellness life of disease prevention through the overall approach to life. Understanding the viewpoint of water, food, and the environment through the lifestyle approach of eight constitutions, and mentioned the following wellness approach. First, it is necessary to establish a health care system that focuses on preventing and managing not only mental personality but also physical personality. And we need to know more about our constitution and human body. Second, it is not the treatment of illness according to the pathological state, but a customized treatment approach called the treatment of other diseases is necessary. Third, the approach to personalized treatment should be centered on the natural management according to the correct constitution. Fourth, it is necessary to control the unnecessary environment by encouraging the energy to become active according to the physical personality. This will be an important source of belief in the self-empowering power of the human body by suggesting the direction of the goal of achieving a wellness life based on the above-mentioned health behavior.

MCT (medium-chain triglyceride) 및 LCT (long-chain triglyceride) 유제가 백서에서 허혈 / 재관류 심장기능손상 및 혈소판응집능에 미치는 영향

이희주(Hee Joo Lee),문창현(Chang Hyun Moon),정이숙(Yi Sook Jung),김민화(Min Hwa Kim),이수환(Soo Hwan Lee),백은주(Eun Joo Baik),홍정(Jeong Hong),왕희정(Hee Jung Wang),김명욱(Myung Wook Kim) 한국응용약물학회 1998 Biomolecules & Therapeutics(구 응용약물학회지) Vol.6 No.4

Intravenous lipid emulsion is used extensively as a major component of parenteral nutrition for patients in the surgical intensive care unit. Abnormal cardiovascular function related to lipid infusion has been reported although conflicting results exist. In the present study, we investigated the effects of intravenous emulsions of long-chain triglyceride (LCT) and medium-chain triglyceride (MCT) on myocardial ischemia/ reperfusion injury and on platelet aggregation in rat. There was no difference between LCT and MCT considering the effects on left ventricular developed pressure (LVDP) and coronary flow rate (CFR) before and after ischemia/reperfusion in isolated rat heart. On the other hand, a difference was found between LCT and MCT with regard to their effects on heart rate (HR) and end diastolic pressure (EDP) after ischemia/ reperfusion. After ischemia/reperfusion, HR was significantly (P<0.05) reduced and EDP significantly (P<0.05) increased by LCT (18±2.0% and 42.8±8.9%, respectively), but not by MCT. Ex vivo platelet aggregation induced by collagen was reduced by LCT infusion, but not by MCT. These findings suggest that MCT may have slightly more favorable effect than LCT on the myocardial function after ischemia/reperfusion in rat.

건강검진센터에서 위장 및 대장 수면 내시경 검사 증가에 따른 필요 회복실 침상 수 및 대기 시간 예측 시뮬레이션

이희주(Hee-Joo Lee) 한국콘텐츠학회 2010 한국콘텐츠학회논문지 Vol.10 No.2

질병의 조기진단을 위한 건강검진이 늘어나면서 상대적으로 불편감이 적은 위장 및 대장 수면 내시경에 대한 요구도가 증가하고 있다. 본 연구는 한 서울 종합병원 건강검진 센터에서의 수면 내시경 검사, 회복 및 회복실 대기 시간을 이용하여 현재 수면 내시경 대상자 및 대상자가 10%, 20% 증가할 경우 필요한 회복실 침상 수 및 회복 대기 시간을 예측하기 위해 실시되었으며 ARENA 10.0 시뮬레이션 프로그램을 이용하여 분석하였다. 현재의 수면 내시경 대상자 수인 경우 회복실 대기시간 7분, 대기 명수 2명, 침상가동률 약 50%이며, 수면 내시경 대상자 수가 10%, 20% 증가함에 따라 각각 대기 시간, 명수 및 침상가동률이 증가하였다. 그러므로 현재 상태 및 대상자 증가 시에도 대상자의 안전관리를 위해 수면 내시경 후 회복실의 침상을 3개 증가시켜야 대기시간이 2분 이내로 줄어드는 것으로 나타났다. The increasing regular medical check up rate for early diagnosis in disease has increasing sleep endoscopy rate because of reduction with discomfort. The purpose of this study was to determine the number of recovery bed as increasing sleep endoscopy rate using check up time, waiting time & recovery time at a general hospital in Seoul. This study was analyzed using ARENA 10.0 program. At present and as increasing of sleep endoscopy rate 10%, 20% was increased recovery time, waiting time & the rate of bed inflection. So at present, the number of recovery bed has to increase for client's safety and as increasing of sleep endoscopy rate 10%, 20% has to increase 3 and then waiting time decreased in 2 minutes.

1-beta-D-Arabinofuranosylcytos의 Prodrug 연구 AraC-5'-Alkylthioacetates 합성 및 그들의 물리.화학적 성질과 항암작용 시험

이희주(Hee Joo Lee),김태련(Tae Ryun Kim) 대한약학회 1988 약학회지 Vol.32 No.5

AraC-5''-methylthioacetate (araC-MTA, 1) and araC-5''-butylthioacetate (araC-BTA, 2) were prepared and their physical and chemical properties and in vivo antitumor activities were examined. Both compounds were found to have higher partition coefficients (n-hexanol/water) than their parent araC and to be hydrolyzed to araC within an hour in mouse plasma and ascitic fluid solutions. In in vivo antitumor activity test they showed similar potency to araC, which were assumed due to too quick hydrolyses of them to parent in the body fluid.