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Cytarabine 으로 유도된 탈모증에 대한 상백피 추출물의 육모촉진 효과
이희삼(Heui Sam Lee),문재유(Jae Yu Moon) 대한약학회 2000 약학회지 Vol.44 No.1
This study was designed to examine the effect of Mori cortex radicis extract on cytarabine induced alopecia in young rat. cytarabine (50mg/kg) was injected to Eight-day-old rats everyday for 7 days. After the injection, Mori cortex radicis extract was spread on the alopecia young rats for 12 days topically. The stimulative effect of hair growth was observed on 12th day of topical application. The stimulative effect of hair growth was best in MEOH extract group. And the same result was obtained in the experiment of hair follicle cross section.
이희삼(Heui-Sam Lee),김선여(Sun-Yeou Kim),이완주(Won-Chu Lee),이상덕(Sang-Duk Lee),문재유(Jae-Yu Moon),류강선(Kang-Sun Ryu) 한국식품영양과학회 2000 한국식품영양과학회지 Vol.29 No.5
흰쥐에게 뽕잎을 4주동안 투여하여 장기능에 미치는 영향을 알아본 결과, 사료이용효율은 10% 투여구에서 14% 감소되었고, 장 통과시간은 1% 투여구에서 16%, 10% 투여구에서 21% 빨라졌다. 장의 길이는 1% 투여구에서 10%, 10% 투여구에서 11% 증가되었고, 장 통과속도는 1% 투여구에서 30%, 10% 투여구에서 40% 빨라졌다. 변의 pH는 대조구가 6.91인데 비해 1% 투여구는 6.07, 10% 투여구는 5.95로서 뽕잎 투여량이 증가함에 따라 변의 pH는 점차 낮아졌다. 변량은 1% 투여구에서 57%, 10% 투여구에서 91% 증가되었다. 변중의 무기성분 중에서 K의 함량이 50% 증가하였으나, 이는 뽕잎 중에 K함량이 많기 때문으로 생각된다. This experiment was carried out to investigate the effects of dietary mulberry leaf powder on gastrointestinal function of rats. Plot containing one and ten percent mulberry leaf powder was supplied with rats. The food efficiency ratio (FER) of rat fed with 10% mulberry leaf powder was decreased 14%. The transit time of rat fed with 1% and 10% mulberry leaf was faster 16% and 21% than that of control respectively. Intestine length was increased 10% and 11% at 1% and 10% dietary mulberry leaf respectively. The transit speed was faster 30% at 1% dieatry mulberry leaf than that of control. The pH of rat's feces suplied with mulberry leaf was lower than that of control. The amount of daily rat's feces fed mulberry leaf was much than that of control. As above results, we suggest that mulberry leaf was evaluated to improve the function of rat's gastrointestine.
사염화탄소에 의해 유발된 간독성에 대한 상백피 추출물의 간보호효과
김선여(Sun Yeou Kim),이희삼(Heui Sam Lee),류강선(Kang Sun Ryu),이은주(Eun Ju Lee),김영중(Young Choong Kim) 대한약학회 1999 약학회지 Vol.43 No.3
In order to evaluate the protective effects of extracts of Mori cortex radicis on carbon tetrachloride induced hepatotoxicity, glutamic oxaloacetic transaminase, glutamic pyruvic transaminase, lactic dehydrogenase, malondialdehyde values and glutathione S-transferase activity were measured in ICR mice. The activities of serum aminotransferase and the hepatic content of lipid peroxide after carbon tetrachloride-treatment were markedly increased than normal control but those levels were significantly decreased by the treatment of butanol fraction of Mori cortex radicis mathanol extract. Glutathione S-transferase activity was decreased by carbon tetrachloride than control, but that also inhibited by the treatment of butanol fraction of Mori cortex radicis methanol extract. These results demonstrate a possible hepatoprotective role of extracts of Mori cortex radicis against CCl4-induced hepatoxicity in vivo.
Calcium Nitrate/에탄올 혼합 용액에 의한 작잠견사(繭絲)의 용해
이광길 ( Kwang Gill Lee ),조유영 ( You Young Jo ),여주홍 ( Joo Hong Yeo ),이희삼 ( Heui Sam Lee ),김기영 ( Kee Young Kim ),박광영 ( Kwang Young Park ),권해용 ( Hae Yong Kweon ) 한국잠사학회 2013 한국잠사곤충학회지 Vol.51 No.1
Antheraea pernyi silkworm is one of typical wild silkworms, which spins a tawny color cocoon. The cocoon has been used as a resource for textile material due to strong chemical stability and good mechanical properties. In this study, to increase the solubility efficiency of A. pernyi silk fibroin, the composition of dissolution solvent were examined. Calcium chloride tertiary system, normally used for dissolution of Bombyx mori silk fibroin, does not act on A. pernyi silk fibroin. Calcium nitrate system dissolves A. pernyi silk fibroin, and calcium nitrate ethanol system do more easily than calcium nitrate system. Amino acid composition of A. pernyi silk fibroin obtained after dissolution is mainly composed of alanine, glycine, and serine. A. pernyi silk fibroin would be used for non-textile applications near future.
작잠 실크 피브로인에 의한 in vitro 상처 회복 효과 및 에탄올 처리에 따른 작잠 실크 피브로인 스폰지의 구조 전이
이광길 ( Kwang Gill Lee ),조유영 ( You Young Jo ),여주홍 ( Joo Hong Yeo ),이희삼 ( Heui Sam Lee ),김기영 ( Kee Young Kim ),김현복 ( Hyun Bok Kim ),김안숙 ( An Sook Kim ),김성곤 ( Seong Gon Kim ),권해용 ( Haeyong Kweon ) 한국잠사학회 2014 한국잠사곤충학회지 Vol.52 No.1
작잠 누에고치를 정련한 후 질산칼슘4수화물의 용융액을 사용하여 재생 작잠 실크피브로인 스펀지를 제조하였다. 작잠 실크피브로인은 280 nm에서 tyrosine 잔기 등에 기인한 흡광대를 나타내었다. 작잠 실크피브로인 스펀지를 에탄올 농도별로 처리한 후 구조 전이를 관찰한 결과 80%에탄올 처리시에는 β-sheet 구조(700 cm-1), α-helix 구조 (625 cm-1), 그리고 random coil (660 cm-1) 구조가 공존하는 것으로 나타났다. 또한 작잠 실크피브로인을 이용한 in vitro 상처회복실험 결과 실크피브로인의 첨가에 의하여 상처회복 효과가 인정되었다. Regenerated Antheraea pernyi silk sponge was prepared using calcium nitrate 4 hydrate melt and examined the conformational changes treated with aqueous ethanol solution. The conformation of silk sponges was changed from random coil structure to β-sheet and α-helix conformation with low ethanol concentration (50 ~ 70%). On the other hand, that of silk sponges with 80% ethanol treatment showed β-sheet (700 cm?1), α-helix (625 cm?1), and random coil (660 cm?1) specific peaks. Wound healing effect in vitro was observed by cytoslective wound healing kit. Therefore, regenerated Antheraea pernyi silk sponges might be used as promising wound dressing materials.
황정욱 ( Jung Wook Hwang ),이희삼 ( Heui Sam Lee ),김호진 ( Ho Jin Kim ),김규오 ( Kyu Oh Kim ),최용수 ( Yong Soo Choi ) 한국잠사학회 2012 한국잠사곤충학회지 Vol.50 No.2
Silk protein has been explored to be used for biomedical applications for several decades. However, it has not been used in this field cause to their irreversible crystallization after dissolving in water. The existing methods of silk protein hydrolysis using silkworm cocoon were used with harmful solvents and through a very complicated process. Therefore, we have developed novel methods for the production of water-soluble hydrolysate using silkworm gland. We manufactured two types of silkworm gland-derived hydrolysate (water-soluble SGH, SSGH; total SGH, TSGH) and compared the characteristics with commercial cocoon-derived sericin hydrolysate (CSH). The molecular weight of SGH ranged from 7 to 50 kDa (SSGH) and 5 to 15 kDa (TSGH) within glycine, alanine, and aspartic acid as a main amino acid composition. In contrast, CSH ranged from 15 to 50 kDa within serine and aspartic acid. The results of FTIR implied that SGH was more soluble form than CSH, as shown by the decrease in the β-sheet structure at 1630 cm-1 on amide I peak. In comparison with 10% fetal bovine serum, 0.1% (1 mg/ml) SSGH had equivalent effect on the proliferation of human dermal fibroblasts and mesenchymal stem cells. All results of the SSGH made by novel manufacturing process indicate the SSGH is more preferable as a culture medium supplement than cocoon-derived sericin.
문지영 ( Ji Young Mun ),이희삼 ( Heui Sam Lee ),이광길 ( Kwang Gill Lee ),권해용 ( Hae Yong Kweon ),조유영 ( You Young Jo ),여주홍 ( Joo Hong Yeo ) 한국잠사학회 2013 한국잠사곤충학회지 Vol.51 No.2
In this study, matured silkworm hemolymph was studied for inhibitory effect on melanin formation, tyrosinase activities. A matured silkworm hemolymph mass purification method was developed and some of suppressing melanin formation effects were investigated, too. As a result, the advanced purification method was higher in yield rate than existing one by 53%. A free radical scavenging activity of hemolymph was higher than sericin on the market (IC50, 202.9 ± 33.1) and lower than vitamin C (0.57 ± 0.02) by 6.4 ± 0.3 μg/mL. An inhibitory effect on melanin synthesis was higher in hemolymph compared to Morus bark and arbutin by 9.15% and 11.56%, respectively. An inhibitory effect on tyrosinase activity was higher in hemolymph than Morus bark and lower than arbutin. Effect of hemolymph 45 μg/mL on suppressing of tyrosinase was almost 90%. Thus, these results suggest that hemolymph of matured silkworm may have beneficial properties as a material for cosmetics.