트리암시놀론이 배양된 사람 섬유아세포의 생존과 산화질소 생성에 미치는 영향

이효원,김재우,Hyo-Won Lee,Jae-Woo Kim 대한안과학회 2005 대한안과학회지 Vol.46 No.11

Purpose: To investigate the effect of triamcinolone acetonide (TA) on cell survival and nitric oxide (NO) production in cultured human Tenon`s capsule fibroblast (FB) cells. Methods: After exposed to TA, indomethacin, and dexamethasone for 2 days, cell survival and nitrite production of primarily cultured human FB cells were assessed with MTT and Griess assays, respectively. Results: Cell survival increased after exposure to TA at low concentrations but decreased at high concentrations. TA decreased the production of NO significantly (p<0.05). Exposure to indomethacin and dexamethasone revealed similar results. Conclusions: Both the biphasic effect on cell survival and the decreased production of NO induced by TA in FB cells could affect conjunctival wound healing

Synthesis of 3,6-Dihydro-2H-pyran Subunits of Laulimalide Using Olefinic Ring Closing Merathesis. PartⅠ

이효원,정충식,윤수혜,이일영최,Lee, Hyo Won,Jeong, Chung Sik,Yun, Su Hye,Lee, Il Yeong Choe Korean Chemical Society 2001 Bulletin of the Korean Chemical Society Vol.22 No.8

솔보써말 방법을 이용한 구리분말 제조 및 전자파 차폐제로의 응용

이효원,김수룡,권우택,최덕균,김영희,Lee, Hyo-Won,Kim, Soo-Ryong,Kwon, Woo-Teck,Choi, Duck-Kyun,Kim, Young-Hee 한국재료학회 2006 한국재료학회지 Vol.16 No.5

Copper powders have been widely used in electrically conductive coatings, electrode materials et al. and are very prospective since they are cheaper than noble metal powders such as silver or palladium. In this study, copper powders for metal filler of EMI shielding have been prepared using a solvothermal process from $CuSO_4$, NaOH, Glucose, mixed solvent ($H_2O$: Ethanol) and hydrazine which was used as a reducing agent at various reaction conditions. The prepared copper powders showed finely dispersed spherical shape without agglomerate, uniform morphology, narrow size distribution, high purity and were about 400-700 nm in size. The prepared powders were characterized using XRD, SEM, TGA, XPS, particle size measurement and EMI shielding efficiency.

나주, 천년의 역사, 그리고 가능성

이효원,Lee, Hyo-Won 대한건축사협회 1999 건축사 Vol.1999 No.5

Synthesis of 3,6-Dihydro-2H-pyran Subunits of Laulimalide Using Olefinic Ring Closing Metathesis.PartⅡ

이효원,윤서혜,최이일영,장봉용,Lee, Hyo Won,Yun, Seo Hye,Choe, Lee Il Yeong,Jang, Bong Yong Korean Chemical Society 2001 Bulletin of the Korean Chemical Society Vol.22 No.11

Synthesis of the C11-C21 and C13-C21 Fragments of Epothilones from D-Glucose

이효원,최일영,홍용덕,Lee, Hyo Won,Choe, Il Yeong,Hong, Yong Deok Korean Chemical Society 2000 Bulletin of the Korean Chemical Society Vol.21 No.12

Synthesis of Chloral-2-acetothienone and Trichloroethylidene-2-acetothienone, and Reaction of Trichloroethylidene-2-acetothienone with Hydrazines

이효원,이윤영,Lee, Hyo Won,Lee, Youn Young Korean Chemical Society 1975 대한화학회지 Vol.19 No.1

2-Acetothienone과 chloral을 축합시켜 chloral-2-acetothienone을 생성하였으며 이것을 탈수하여 trichloroethylidene-2-acetohienone을 합성하였다. Trichloroethylidene-2-acetothienone과 phenylhydrazine 또는 치환된 phenylhydrazine들과의 반응으로 2-aryl-6-(2-thienyl)-3(2H)-pyridazinone들을 얻었으며 hydrazine과의 반응에서는 3-(2-thienyl)-5-trichloromethyl-2-pyrazoline이 합성되었다. Chloral-2-acetothienone was synthesized from the condensation of 2-acetothienone with chloral, and trichloroethylidene-2-acetothienone was obtained from the dehydration of chloral-2-acetothienone. From the reaction of trichloroethylidene-2-acetothienone with phenylhydrazine or substituted phenylhydrazines 2-aryl-6-(2-thienyl)-3(2H)-pyridazinones were obtained. 3-(2-Thienyl)-5-trichloromethyl-2-pyrazoline was synthesized from the reaction of trichloroethylidene-2-acetothienone with hydrazine hydrate.

답리작 춘계포장에서 보리 및 이탈리안 라이그라스와 두과의 혼파비율이 동위원소 희석법 및 차이법을 이용한 질소고정 및 이동에 미치는 영향

이효원,이효진,김원호,윤봉기,고한종,Lee, Hyo-Won,Lee, Hyo-Jin,Kim, Won Ho,Yoon, Bong Ki,Ko, Han Jong 한국초지조사료학회 2016 한국초지조사료학회지 Vol.36 No.4

In order to study the effect of barley, Italian ryegrass (IRG), and legume mixture on nitrogen fixation and transfer to grasses on spring paddy field, an experiment was carried out from Oct. 2006 to June 2007 in Naju, Korea. A split plot design with three replications was used for the experiment. One reference plot was assigned for each treatment to determine nitrogen fixation. Main plots consisted of Chinese milk vetch, crimson clover, forage pea, and hairy vetch with barley, respectively. Subplot treatment were barley or IRG with four seeding ratio of legumes (50:50, 60:40, 70:30, and 80:20). To estimate N fixation by legumes, $^{15}N$ isotope dilution technique was used. $^{15}N$ fertilizer [$(^{15}NH_4)_2SO_4$ solution at 99.8 atom N] was uniformly applied to $600cm^2$ in the middle of each plot on April 15, 2007. Plots were harvest by hand on June 8, 2007. Dried sample were ground to a fine power and analyzed for total N isotope N. $^{15}N$ was determined using elemental analyzer-isotope ratio mass spectrometry. The calculation of N transfer was determined with the isotope dilution method. The content of N was higher in legumes than that in barley or Italian ryegrass. Nitrogen level in forage pea was significantly higher than that of other legumes. There were significantly differences in N content between legumes in IRG mixture. Atom % $^{15}N$ excess was significantly different in legumes with barley. The 60:40 sub plot had higher (p<0.05) atom % $^{15}N$ than other seeding ratio treatments. The enrichment ranged from 0 to 0.58. Compared to barley, the enrichment of IRG with its accompanied legumes was higher, ranging from 0.38 to 1.0. The N derived from the atmosphere (Ndfa) ranged from 0% to 49.5% with barley-legume mixture. It ranged from 0 to 60.5% in IRG-legume plots. N transfer from legumes to neighboring grasses was 12.3 to 90.9 kg/ha for barley-legume mixture and 31.7 to 107.8 kg/ha for IRG plots. IRG plots showed higher N transfer for IRG-legume mixture in general based on difference method. Based on $^{15}N$ dilution method, the N transfer was 0 to 36.1 kg/ha for barley-legume mixture and 0 to 50.6 kg/ha for IRG plots. There was a tendency toward higher N transfer on the difference method than that of the $^{15}N$ dilution method.

근적외선 분광법에 의한 사초의 성분추정

이효원(Hyo-Won Lee),김종덕(Jong Duk Kim),김원호(Won Ho Kim),이종경(Joung Kyong Lee) 한국초지조사료학회 2009 한국초지조사료학회지 Vol.29 No.1

본 연구는 청예용 사초의 질을 신속하고 정확하게 측정할 수 있는 대안을 모색하기 위하여 수행하였다. 근적외선분광분석법을 이용한 사초의 분석가능성을 타진하기 위해 2002년부터 2007년에 거쳐 생산된 각종 사초 258점을 시료로 사용하였다. 즉 시료는 조단백질, 조섬유, NDF, ADF 그리고 건물소화율을 분석하였으며, 또한 NIR System으로 400~2,400㎚ 사이의 파장을 얻었다. 그리고 파장과 습식분석치를 이용하여 중회귀식을 만들고 이것을 사용하여 미지의 시료를 분석할 수 있는가를 검증하였다. 근적외선 분석법의 중요한 지표인 결정계수 r²와 표준오차이며 본 실험의 결과 검증식의 r²는 CP (crude protein), CF (crude fiber), ADF (acid detergent fiber), NDF (neutral detergent fiber) 그리고 IVTD (in vitro true digestibility)에서 각 각 0.70, 0.86, 0.94, 0.94 그리고 0.89였다. 검량식은 그 값이 0.47, 0.39, 0.89, 0.90 그리고 0.61이었다. 본 실험 결과 근적외선분광법에 의한 청예사료 질평가가 가능하며 특히 조섬유, ADF 그리고 진정인비트로 소화율 (IVTD)에서 유효할 것으로 나타났다. 그러나 보다 정확한 결과를 얻기 위해서는 시료는 한국사초의 대표성을 갖는 것을 수집하여 사용해야 할 것이다. 앞으로 더 많은 시료를 추가하면 모든 사초를 분석할 강고한 검량식이 작성될 수 있을 것으로 기대된다. This study was conducted to find out an alternative way of rapid and accurate analysis of forage quality. Near reflectance infrared spectroscopy (NIRS) was used to evaluate the possibility of forage analysis and collect 258 samples such as barley for whole crop silage, forage corn and sudangrass from 2002 to 2007. The samples were analyzed for CP (crude protein), CF (crude fiber), ADF (acid detergent fiber), NDF (neutral detergent fiber) and IVTD (in vitro true digestibility), and also scanned using NIRSystem with wavelength from 400~2,400㎚. Multiple linear regression was used with wet analysis data for developing the calibration model and validate unknown samples. The important index in this experiment was SEC and SEP. r² for CF, CP, NDF, ADF and IVTD in calibration set were 0.70, 0.86, 0.94, 0.94 and 0.89, also 0.47, 0.39, 0.89, 0.90 and 0.61 in validation sample, respectively. The results of this experiment indicates that NIRS was reliable analytical method to assess forage quality, specially in CF, ADF and IVTD, sample should be included for respective forage samples to get accurate result. More robust calibrations can be made to cover every forage samples if added representative sample set.

춘계답리작 토양에서 두과의 질소고정과 이의 화본과로의 이동

이효원(Hyo Won Lee) 韓國草地學會 2007 한국초지조사료학회지 Vol.27 No.3

분뇨이용에 대한 관심이 높아지면서 질소고정을 통해 동반하는 작물에 질소를 고정하고 이동하는 두과와 화본과의 혼파가 주목을 받고있다. 본 연구는 각기 다른 혼파조합이 춘계논 토양에서 두과작물의 질소고정과 이의 이동을 규명하고자 실시하였다. 시험처리는 난괴법 3처리, 3반복이었으면 세 개의 다른 혼파처리는 호밀+벳치, 이탈리안라이그래스+크림손클로버 그리고 연맥+완두였다. 충남 서천의 논토양을 포트에 채워 시험을 하였다. 호밀과 헤어리벳 치구는 수원의 진흥청 포장에서 이식하였고 나머지 두 처리는 3월 초에 각기 다른 화본과 두과의 비율로(이탈리안라이그래스:크림손클로버 =7:3, 연맥:완두=6:4)포트에 파종하였다. 99.8%의 질소동위원소{(15 NH₄)₂SO₄ solution at. 99.8 atom%15N}는 ㏊당 2㎏의 수준으로 각 포트에시용하였는데 호밀과 벳치는 4월 6일 그리고 나머지 두 처리는 4월 16일에 처리하였다. 각 포트에서 출수기에 수확하여 화본과와 두과로 분리한 다음 총질소와 15N값은 서울대학교부설 나이샘(NICEM)의 매스 스팩트로메트리로 분석하였다. 호밀+헤어리벳치, 이탈리안라이그래스+크림손클로버 그리고 연맥+완두 처리의 건물함량은 각각 6,607㎏, 3,212㎏ 그리고 4,312㎏였다. 공중질소에서 기인한 질소의 비는 0.73(호밀+벳치), 0.42(이탈리안라이그래스+크림손클로버) 그리고 0.93(연맥+완두)였다. 두과에서 화본과로의 질소이동비율은 차이법에서 61%에서 4% 사이였고, 동위원소법에서는 12%에서 ?32% 범위였다. Manure recycle is an emerging issue in agriculture in Korea these days. Farmers are keeping eye on legume mixture with grasses for nitrogen fixation and transfer to companion crops by legumes. We had a trial to investigate the effect of different legume mixtures on nitrogen fixation and transfer in spring soil. The treatment was arranged in a randomized complete block design with three replications. Three different mixtures were used(rye+hairy vetch, Italian ryegrass+crimson clover, oat+pea) and sowed in pots with paddy soil from western part of Korea(Seo Chon County). Pots with rye+vetch were transplanted from field of RDA(rural development administration) in Suwon on 2 February 2007 and other mixture treatments were sowed on early March with different sowing rate(7:3=Italian:crimson, 6:4=oat:pea). ( 15 NH₄)₂SO₄ solution at. 99.8 atom %¹?N was applied to the each pot at the rate of 2㎏ N ㏊?¹. Application was done on 6 April at rye+vetch pots and remainder were applied on April 16. Forage were harvested from each pot at ground level in heading stage and separated into legume and grass. Total N content and 15 N value were determined using a continuous flow stable isotope ratio mass spectrometry. DM yield of rye+vetch, Italian+crimson and oat+pea were 6,607, 3,213 and 4,312 ㎏/㏊, respectively. Proportion of N from fixation was 0.73(rye+vetch), 0.42(Italian+crimson) and 0.93(oat+pea). The percentages of N transfer from legume to grass were from 61 % to 24 % in different method by treatment and ?35% to 21 % in isotope dilution method.