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Hussain Muhammad,이해속,Badri Anarjan Mahdi,이상협 한국원예학회 2024 Horticulture, Environment, and Biotechnology Vol.65 No.2
The genus Brassica includes vegetables such as broccoli, cabbage, caulifl ower, kale, and kohlrabi, which are economi cally important because they contain benefi cial compounds, such as glucosinolates. Clustered regularly interspaced short palindromic repeats-Cas9 (CRISPR-Cas9) is a new breeding technology that is expected to replace traditional breeding technologies because it generates transgene-free lines with improved traits. Therefore, protoplast-mediated regeneration methods need to be developed. To date, several methods for isolating and regenerating protoplasts have been identifi ed in Brassica vegetables. However, the effi ciency of these methods varies between species and genotypes. Furthermore, proto plast regeneration effi ciency was found to not be suffi cient for the routine application of CRISPR-Cas9 technology. In this study, we identifi ed that the third and fourth true leaves of broccoli, cabbage, caulifl ower, kale, and kohlrabi were good sources for the isolation of intact protoplasts. In addition, modifi ed protoplast isolation and microcallus culture protocols were optimized for these vegetables. Furthermore, a regeneration protocol for true leaf-originating protoplasts in cabbage and caulifl ower was developed and optimized. We believe that this protocol could be applied to other Brassica vegetables such as broccoli, kale, and kohlrabi.
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잡초 제거에 의한 Miscanthus × giganteus의 바이오매스 증가: 잡초와의 조기 자원 경쟁 우위의 중요성
한경덕,이상협,김윤하,이해속 강원대학교 농업생명과학연구원 2022 강원 농업생명환경연구 Vol.34 No.3
Silver grass (Miscanthus × giganteus) has traditionally been used as a feedstock and for ornamental and medicinal purposes. Recently, however, the biomass uses of silver grass have also been highlighted. This grass comprises 40% cellulose, 25% hemicellulose, and 21% lignin, the cellulose component of which can be converted into glucose, as a source of biofuel, whereas the lignin can be used as a resource for UV protectant film development. Given these utilities, silver grass is considered to have high value as a biomass plant. Consequently, it is desirable to develop efficient methods for the cultivation of this grass. Some studies have reported that weed control is necessary during the initial 2 years of silver grass cultivation; however, none of these studies have been conducted in a Korean cultivation environment. In this study, we accordingly evaluated the efficacy of four herbicides commonly used in Korea for weed control during silver grass cultivation. We found that herbicide treatments have beneficial effects on the growth of silver grass, notably with respect to branching. On the basis of our findings, we conclude that to maximize the final biomass yield, a high level of weed control is essential during the initial 2 years of silver grass cultivation.
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