Stimulus-Responsive Conformational Conversion for Triggered Cytotoxicity of Peptides

이한울,이정훈,오은택,최민혁,김하경,김지원,박헌주,김철희 한국고분자학회 2019 한국고분자학회 학술대회 연구논문 초록집 Vol.44 No.1

신 공여자에서 신장 깊이 측정에 따른 사구체여과율의 비교에 관한 고찰

이한울,박민수,강천구,조석원,김주연,권오준,임한상,김재삼,박훈희,Lee, Han Wool,Park, Min Soo,Kang, Chun Goo,Cho, Seok Won,Kim, Joo Yeon,Kwon, O Jun,Lim, Han Sang,Kim, Jae Sam,Park, Hoon-Hee 대한핵의학기술학회 2014 핵의학 기술 Vol.18 No.2

$^{99m}Tc$-DTPA 신장 신티그래피는 신공여자에게서 사구체 여과율을 평가하고, 신장 이식 가능여부를 결정하는데 있어 중요한 지표가 된다. 사구체 여과율을 측정하는데 이용되는 Gates법은 신장 깊이, 주사량, 순 신장 계수의 3가지 변수들을 고려해야 한다. 본 연구에서는 3가지 변수 중 신장 깊이 측정방법에 따른 신공여자의 사구체 여과율 변화를 비교 평가하고자 한다. 2013년 10월부터 2014년 3월까지 본원에 내원하여 복부 CT와 $^{99m}Tc$-DTPA 사구체 여과율 검사를 시행한 32명의 신공여자를 대상으로 하였다. CT에서의 횡단면 영상과 감마카메라에서 획득한 측면 영상으로 신장 깊이를 측정하고, 신장 깊이 산출 공식인 Tonnesen, Taylor, Itoh법과 비교하였다. GE사의 Xeleris Ver. 2.1220을 이용하여 신장 깊이에 따른 사구체 여과율을 산출한 후, 혈청 크레아티닌 수치를 이용한 MDRD(Modification of Diet Renal Disease) 사구체 여과율과 비교 분석하였다. CT와 감마카메라 영상에서 측정한 신장 깊이는 높은 상관관계를 보였다. 사구체 여과율은 Tonessen 공식을 적용하여 산출한 값이 최소치로 나타났고, CT 영상에서의 신장 깊이를 대입하여 산출한 값이 최대치로 나타났으며, 이를 적용한 사구체 여과율은 16.62%의 차이를 보였다. MDRD 사구체 여과율은 Taylor, Itoh, CT 및 감마카메라에서의 신장 깊이를 적용한 값에서는 통계적으로 유의한 차이를 보이지 않았으나 (p>0.05), Tonnesen 공식을 적용하여 산출한 값에서는 유의하게 나타났다(p < 0.05). CT 측정값을 대입하여 산출한 사구체 여과율 또한 MDRD, Taylor, Itoh, 감마카메라에서의 측정 깊이를 적용한 산출값과는 통계적으로 유의한 차이를 보이지 않았으나(p > 0.05), Tonnesen 공식을 적용하여 산출한 값에서는 유의하게 나타났다(p < 0.05). 본 연구에서는 신공여자에게서 $^{99m}Tc$-DTPA를 이용한 사구체 여과율 평가 시 Tonnesen 공식을 적용한 Gates법이 MDRD 사구체 여과율에 비하여 과소평가됨을 알 수 있었다. 따라서, MDRD 사구체 여과율과 검사 결과 산출된 값이 큰 차이를 보이는 경우 기존 Gates법에 적용되는 Tonnesen 방정식을 대신하여 Itoh 방정식을 적용 하거나 영상을 기반으로 측정한 신장 깊이를 적용하면 보다 정확한 사구체 여과율 평가가 가능할 것으로 사료된다. Purpose $^{99m}Tc$-DTPA renal scintigraphy serves as a key indicator to measure a kidney donor's Glomerular Filtration Rate (GFR) and determine the possibility of kidney transplant. The Gates method utilized to measure GFR considers 3 variables of renal depth, injection dose, and net kidney counts. In this research, we seek to compare changes in kidney donors' GFR according to renal depth measurement methods of the 3 variables. Materials and Methods We investigated 32 kidney donors who had visited the hospital from October, 2013 to March, 2014 and received abdominal CT and $^{99m}Tc$-DTPA GFR examination. With the cross-section image of the CT and the lateral image from a gamma camera, we measured the renal depth and compared with renal depth calculation equations-Tonnesen, Taylor, and Itoh methods. Renal depth-specific GFR was calculated by using Xeleris Ver. 2.1220 of GE. Then the results were compared with MDRD (Modification of Diet Renal Disease) GFRs based on serum creatinine level. Results The renal depths measured based on the CT and gamma camera images showed high correlation. Tonessen equation gave the lowest GFR value while the value calculated by using the renal depth of CT image was the highest with a 16.62% gap. MDRD GFR showed no statistically significant difference among values calculated through Taylor, Itoh, CT and gamma camera renal depth application (P>0.05), but exhibited a statistically significant change in the value based on Tonnesen equation (P<0.05). Conclusion This research has found that, in GFR evaluation in kidney donors by utilizing $^{99m}Tc$-DTPA, Tonnesen equation-based Gates method underestimated the value than the MDRD GFR. Therefore, if a MDRD GFR value shows a huge difference from the actual examination value, using an image-based renal depth measurement, instead of Tonnesen equation applied to Gates method, is expected to give an accurate GFR value to kidney donors.

PET/CT 검사 시 호흡에 따른 영상 왜곡 감소를 위한 엎드린 자세의 유용성 평가

이한울,김정열,최용훈,임한상,김재삼,Lee, Han Wool,Kim, Jung Yul,Choi, Yong Hoon,Lim, Han Sang,Kim, Jae Sam 대한핵의학기술학회 2019 핵의학 기술 Vol.23 No.1

PET/CT 검사 시 호흡에 따른 움직임은 영상의 인공물과 PET과 CT영상의 정합 오차를 발생시키는 요인으로 작용된다. 크기가 작거나 폐의 기저 부위에 위치한 종양일수록 호흡으로 인한 위치 변위와 왜곡의 정도가 크며 SUV(Standard Uptake Value)와 병소의 부피 측정에 영향을 미치게 된다. 본 연구는 PET/CT 검사 시 엎드린 자세가 호흡에 따른 영상 왜곡의 보정에 유용성이 있는지 알아보고자 한다. 장비는 PET/CT Discovery 600 (GE Healthcare, MI, USA)를 사용하였고, 2018년 5월부터 8월까지 폐 중엽과 하엽에 병소가 확인된 20명을 대상으로 하였다. 바로 누운 자세에서 전신 영상을 획득한 후 동일한 조건으로 엎드린 자세에서 병소 부위의 추가 영상을 획득하였다. 각 영상에서 병소의 SUVmax, SUVmean, 부피를 측정하고 PET과 CT영상에서 병소의 위치 변위를 측정하여 비교 분석하였다. 바로 누운 자세 영상에서 병소의 SUVmax는 $4.72{\pm}2.04$, SUVmean은 $3.10{\pm}1.38$, 부피는 $4.68{\pm}3.20$, 위치 변위는 $4.64{\pm}1.88$로 측정되었고, 엎드린 자세 영상에서 SUVmax는 $5.89{\pm}2.42$, SUVmean은 $3.97{\pm}1.65$, 부피는 $2.13{\pm}1.09$, 위치 변위는 $2.24{\pm}0.84$로 측정되었다. 엎드린 자세 영상의 모든 병소에서 SUVmax, SUVmean이 높게 나타났고, 병소의 부피와 위치 변위는 낮게 측정되었다(p<0.05). 본 연구에서 엎드린 자세의 PET/CT 촬영이 병소의 SUV를 증가시키고 PET과 CT영상의 불일치로 인한 호흡 인공물을 감소 시켜 영상의 질을 향상시킨다는 것을 알 수 있었다. 임상에서 폐 기저부 병소의 불일치를 보정하고 인공물을 줄이기 위한 여러가지 방법 중 경제적이고 효율적인 방법으로 진단에 도움을 줄 것이라 사료된다. Purpose The motion due to respiration of patients undergoing PET/CT is a cause of artifacts in image and registration error between PET and CT images. The degree of displacement and distortion for tumor, which affects the measurement of Standard Uptake Value (SUV) and lesion volume, is especially higher for tumors that is small or located at the base of lungs. The purpose of this study was to evaluate the usefulness of prone position in the correction of image distortion due to respiration of patients in PET/CT. Materials and Methods The imaging equipment used in this study was PET/CT Discovery 600 (GE Healthcare, MI, USA). 20 patients whose lesions were identified in the middle and lower lungs from May to August 2018 were enrolled in this study. After acquiring whole body image in the supine position, additional images of the lesion area were obtained in the prone position with the same conditions. SUVmax, SUVmean, and volume of the lesion were measured for each image, and the displacement of the lesion on PET and CT images were measured, compared, and analyzed. Results The SUVmax, SUVmean, and volume, and displacement of the lesion were $4.72{\pm}2.04$, $3.10{\pm}1.38$, $4.68{\pm}3.20$, and $4.64{\pm}1.88$, respectively for image acquired in the supine position and $5.89{\pm}2.42$, $3.97{\pm}1.65$, $2.13{\pm}1.09$, and $2.24{\pm}0.84$, respectively for image acquired in the prone position, indicating that, for all the lesions imaged, SUVmax and SUVmean were higher and volume and displacement were smaller in the images acquired in prone position compared to those acquired in supine one(p<0.05). Conclusion These results showed that the prone position PET/CT imaging improves the quality of the image by increasing the SUV of the lesion and reducing the respiratory artifacts caused by registration error between PET and CT images. It is considered that the PET/CT imaging in the prone position is helpful in the diagnosis of the disease as an economical and efficient methods that correct registration error for the lesions in basal lung and reduce artifacts.

CNC 가공의 정밀 절삭 시뮬레이션 및 이송속도 스케줄링 시스템 개발

이한울,고정훈,조동우 한국공작기계학회 2004 한국공작기계학회 춘계학술대회논문집 Vol.2004 No.-

This paper presents an accurate cutting simulation and feedrate scheduling system for CNC machining. This system is composed of a cutting simulation part and a feedrate scheduling part. The cutting simulation part computes the geometric informations and calculates the cutting forces in CNC machining. The cutting force model using cutting-condition-independent coefficients was introduced for flat end milling and ball end milling. The feedrate scheduling part divides original blocks of NC code into smaller ones with optimized feedrates to adjust the peak value of cutting forces to reference forces. Some machining examples show that the developed system can control the cutting force at desired levels.

Stimuli-Responsive Conformational Change of KLA peptide with Azobenzene Stapling

이한울,이정훈,최민혁,강태형,김철희 한국고분자학회 2019 한국고분자학회 학술대회 연구논문 초록집 Vol.44 No.2

소형 공작기계의 이송계 시뮬레이션을 이용한 마이크로 엔드밀링의 절삭 공정 시뮬레이션

이한울,정영훈,조동우,박종권 한국공작기계학회 2006 한국공작기계학회 추계학술대회논문집 Vol.2006 No.-

In order to predict the cutting forces in general micro-end-milling operated by NC code, a cutting process simulation model was conducted. This model computes the cutting configurations using a geometric simulation based on ME Z-map and predict the cutting force using a mechanistic cutting force model that can precisely predict the cutting force in micro-end-milling under various cutting conditions. In order to eliminate a serious difference between an actual machine tool behavior and an ideal behavior commanded by NC code, a feed drive simulation was introduced. The comparison of the predicted and measured cutting forces in micro-packet-milling showed that the proposed method provides very accurate results.

NUMERICAL METHOD FOR SIMULATING TIRE ROLLING NOISE BY THE CONCEPT OF PERIODICALLY EXCITING CONTACT FORCE

이한울,조진래,정의봉 한국자동차공학회 2017 International journal of automotive technology Vol.18 No.5

For the numerical simulation of tire rolling noise, an important subject is the extraction of normal velocity data of the tire surface that are essential for the acoustic analysis. In the current study, a concept of periodically exciting contact force is introduced to effectively extract the tire normal velocity data. The ground contact pressure within contact patch that is obtained by the static tire contact analysis is periodically applied to the whole tread surface of stationary tire. The periodically exciting contact forces are sequentially applied with a time delay corresponding to the tire rolling speed. The tire vibration is analyzed by the mode superposition in the frequency domain, and the acoustic analysis is performed by commercial BEM code. The proposed method is illustrated through the numerical experiment of 3-D smooth tire model and verified from the comparison with experiment, and furthermore the acoustical responses are investigated to the tire rolling speed.

절삭력 계수를 통한 마이크로 가공의 절삭 특성 분석

이한울,조동우,박종권 한국공작기계학회 2005 한국공작기계학회 춘계학술대회논문집 Vol.2005 No.-

The complex three-dimensional miniature components are needed for a wide range of applications from the aerospace to the biomedical industries. To manufacture these products, micro machining that can make a high aspect ratio part and has good accuracy is widely researched. In this paper, cutting characteristics were analyzed in micro machining using cutting force coefficients, which are the specific cutting force for normal and frictional direction of rake surface. From measured cutting force in micro end milling, cutting condition independent cutting force coefficients were determined and used for analysing the characteristics of micro cutting. Using the cutting force coefficient, 써써써

엔드밀링 공구의 유한요소해석을 통한 이송속도 스케줄링의 기준 절삭력 산출

이한울,조동우 한국공작기계학회 2004 한국공작기계학회 추계학술대회논문집 Vol.2004 No.-

Off-line feedrate scheduling is presented as the advanced technology to regulate cutting forces at the desired level through change of feedrates. In rough cutting, the feedrate scheduling aims at reducing the machining time, which is the most important factor for better productivity. Thus, the largest force which can avoid breakage of tool shank and tooth is a reference force for feedrate scheduling in rough cutting. In this paper, a calculation method of the reference cutting force for feedrate scheduling is developed. This model calculates rupture plane of tooth using the FEM analysis of a tool and computes the reference force using the transverse rupture strength of a tool. Experiments validate that the presented feedrate scheduling model reduced machining time drastically and regulate cutting forces at the reference cutting force.

Environmental Factors Affecting Establishment and Expansion of the Invasive Alien Species of Tree of Heaven (Ailanthus altissima) in Seoripool Park, Seoul

이한울,이창석 한국통합생물학회 2006 Animal cells and systems Vol.10 No.1

Tree of heaven (Ailanthus altissima Swingle) as an invasive alien plant, appeared usually in the disturbed locations such as road-side, incised slope, and trampling path-side. They appeared abundantly in the trampling path-side but they did not appear or were rare in the interior of forest. Density and importance value of tree of heaven were proportionate to the relative light intensity measured according to distance from the trampling path toward forest interior and closely related to the breadth of trampling path as well. They were associated with annual, other exotic species or ruderal plants well. Distributional pattern of mature trees of them in the study area and its surrounding environments implied that they were introduced intentionally. Size class distribution of them showed that they are in expansion and artificial interferences such as, installing physical training space and developing hiking course functioned as trigger factors in their invasion and expansion. The results support the facts known generally in relation to invasion and expansion of the invasive alien plants. In this viewpoint, we suggest a management plan that applies ecological restoration principles to address ecosystems infected with tree of heaven by restoring the integral feature of the degraded nature and conserving the remained nature more thoroughly.