L - Ascorbic Acid 가 Guinea Pig 에서 혈장 지질과산화값 , Prostaglandin 생합성 , 광용혈 , Superoxide Dismutase 및 Catalase 활성에 미치는 영향

이정원,이태녕,모수미,이진호,이대형,박수남,이보경 ( Joung Won Lee,Tae Young Lee,Sumi Mo,Jin Ho Lee,Dae Hyung Lee,Soo Nam Park,Bo Kyung Lee ) 생화학분자생물학회 1987 BMB Reports Vol.20 No.4

Three levels of L-ascorbic acid(AsA) were orally given to 20 male Hartley guinea pigs weighing average 428 g for five weeks. AsA level was 0, 2.5, and 100 mg per 100 g body weight per day in the deficient, the adequate, and the excess group, respectively. Plasma lipid peroxide value(TBA value) of deficient was increased to the highest among the three groups and both the adequate and the excess groups exhibited the lower TBA values, suggesting the antioxidant action of AsA. In the excess of AsA, plasma TBA value was greatly lowered one day after the feeding, but TBA value after 5 weeks was elevated comparable to that of the adequate. Aspirin administrating experiment suggested that this elevation was due to increased. production of prostaglandin(PG) endoperoxides induced by excess AsA. Thus it could be concluded that excess AsA actually inhibited the tissue lipid peroxidation in vivo. The activities of both superoxide dismutase(SOD) and catalase in erythrocytes demonstrated the elevations in the deficient comparing with the auequate, but not any significant variations in the excess. SOD activities were correlated with TBA value in liver (r = .324) and with percent hemolysis in erythrocytes (r = .323). Photohemolysis of erythrocyte was increased not only in the AsA deficient but also in the excess comparing with the adequate. A new finding that the large excess administration of AsA in guinea pig demonstrated a significant stimulation of PG endoperoxide biosynthesis, would suggest an important biochemical implication on vitamin C as an activator of phospholipase A₂ and/or PG endoperoxide synthetase. The stimulation of PG biosynthesis might be associated with the enhanced photohemolysis, and this problem needs to be further investigated.

L-Ascorbic Acid가 Guinea Pig에서 혈장 지질과산화값, Prostaglandin 생합성, 광용혈, Superoxide Dismutase 및 Catalase활성에 미치는 영향

이정원,이태녕,모수미,이진호,이대형,박수남,이보경,Lee, Joung-Won,Lee, Tae-Young,Mo, Su-Mi,Lee, Jin-Ho,Lee, Dae-Hyung,Park, Soo-Nam,Lee, Bo-Kyung 생화학분자생물학회 1987 한국생화학회지 Vol.20 No.4

L-Ascorbic acid(AsA)를 결핍, 석정, 과잉(적정의 40배)의 세 수준으로 나누어 guinea pig(평균체중 428g, male)에게 5주동안 경구 투여했을때, 적정 및 과잉으로 공급된 AsA는 AsA 결핍군에서 나타난 혈장 및 간조직의 매우 상승된 지질과산화값(TBA값)을 저하시켰으며 이 효과는 과잉공급시 더욱 컸다. 이는 AsA가 항산화작용을 통해 지질 과산화를 억제하였음을 의미한다. 그러나 과량군의 혈장 TBA값은 적정군의 혈장 TBA값 수준에 머물렀는데 이는 과잉의 AsA가 동시에 혈장 TBA값에 포함되어지는 prostaglandin (PG) endoperoxide의 생합성을 촉진했기 때문이다. 그리고 superoxide dismutase(SOD)와 catalase 활성은 AsA결핍시 특히 적혈구에서 증가경향을 보였으나 과잉 공급인 경우엔 별 변화가 없었다. 동물 개개의 SOD활성도는 간조직에서는 TBA값과 (r = .324) 적혈구에서는 용혈수준과 (r = .323) 상관관계 (p < 0.1)가 있었다. AsA의 대량공급이 지질의 과산화반응을 억제한다는 의의 이외에 PG생합성의 촉진수단으로서 중요한 구실을 할 수 있다는 주목할만한 vitamin C의 새로운 생화학적 의의를 시사한다. 또한 AsA의 과량공급은 적혈구의 $^1O_2$에 의한 광용혈을 적정량 공급군에 비해 촉진시키는 결과를 보이고 있다. 이 광용혈 촉진의 생화학적 의의는 PG endoperoxide 생합성 활성화의 결과와 연관성이 있을 것으로 보이며 PG endoperoxides 촉진문제와 더불어 앞으로 검토해야 될 문제로 사료된다. Three levels of L-ascorbic acid(AsA) were orally given to 20 male Hartley guinea pigs weighing average 428 g for five weeks. AsA level was 0, 2.5, and 100 mg per 100g body weight per day in the deficient, the adequate, and the excess group, respectively. Plasma lipid peroxide value(TBA value) of deficient was increased to the highest among the three groups and both the adequate and the excess groups exhibited the lower TBA values, suggesting the antioxidant action of AsA. In the excess of AsA, plasma TBA value was greatly lowered one day after the feeding, but TBA value after 5 weeks was elevated comparable to that of the adequate. Aspirin administrating experiment suggested that this elevation was due to increased. production of prostaglandin(PG) endoperoxides induced by excess AsA. Thus it could be concluded that excess AsA actually inhibited the tissue lipid peroxidation in vivo. The activities of both superoxide dismutase(SOD) and catalase in erythrocytes demonstrated the elevations in the deficient comparing with the auequate, but not any significant variations in the excess. SOD activities were correlated with TBA value in liver (r = .324) and with percent hemolysis in erythrocytes (r = .323). Photohemolysis of erythrocyte was increased not only in the AsA deficient but also in the excess comparing with the adequate. A new finding that the large excess administration of AsA in guinea pig demonstrated a significant stimulation of PG endoperoxide biosynthesis, would suggest an important biochemical implication on vitamin C as an activator of phospholipase $A_2$ and/or PG endoperoxide synthetase. The stimulation of PG biosynthesis might be associated with the enhanced photohemolysis, and this problem needs to be further investigated.

혈청 지질단백질중의 2-Thiobarbituric Acid 반응물질에 미치는 $Fe^{3+}$의 영향

이정원,모수미,이태녕,Lee, Joung-Won,Mo, Su-Mi,Lee, Tae-Young 생화학분자생물학회 1983 한국생화학회지 Vol.16 No.4

2-Thiobarbituric acid (TBA) test, as indirect method, has recently been used for the determination of lipid peroxidation in biological systems including blood. Together with the low sensitivity, lacks of the specificity and the understanding the reaction mechanism, however, have limited the usefullness of TBA test. Under the nitrogen atmosphere $FeCl_3$ showed great enhancement effect on the sensitivity of TBA reaction with serum protein precipitate involving lipid peroxides, but not with tetraethoxypropane. Thus ferric ion was thought to catalize the releasing TBA reactive chromogenic substance, malondialdehyde, from its precursors. The amount of ferric ion required seemed to be proportional to that of serum tested. Also, oxygen should be eliminated in serum TBA reaction so that the reaction could reach at stabe maximum and keep its plateau value. Under anaerobic atmosphere, albumin and amino acids (methionine and glutamic acid) may not contribute significantly to the great enhancement of serum TBA value induced by ferricion.

연구논문 : 서울지역 겨울철 기온과 노인의 사망률간의 관련성 연구(1992년~2007년)

이정원 ( Joung Won Lee ),전형진 ( Hyung Jin Jeon ),조용성 ( Yong Sung Cho ),이철민 ( Cheol Min Lee ),김기연 ( Ki Youn Kim ),김윤신 ( Yoon Shin Kim ) 한국환경영향평가학회 2011 환경영향평가 Vol.20 No.5

This study was investigated the relationship between the temperature and the mortality of aged (≥65 yr) during the winter seasons from 1992 to 2007 in Seoul, Korea by utilizing climate data and death records. The study also estimated the future risks by employing the projections of the population in Seoul, Korea and climate change scenario of Korea from 2011 to 2030. The limitation of this study was the impossibility in the prediction of daily mortality counts. Therefore, daily death numbers could be predicted based on the future population projection for Korea and the death records of 2005. The result indicated that risks increased by 0.27%, 0.52%, 0.32% and 0.41% in association with the 1˚C decrease in daily minimum temperature from the mortality counts of total, respiratory, cardiovascular, and cardiorespiratory in the past date while 0.31%, 0.42%, 0.59% and 0.66% in the future. Based on the results obtained from this study, it is concluded that the risk in the future will be higher than the past date although there is an uncertainty in estimating death counts in the future.

연구논문 : 평택지역 사춘기 여학생의 철분 영양 상태 및 식이 철분 흡수율

이정원(Joung Won Lee),박춘근(Chun Keun Park) 충남대학교 생활과학연구소 1991 忠南生活科學硏究誌 Vol.4 No.-

에탄올 공급이 흰쥐 조직중의 Glutathione 및 지질산화 수준에 미치는 영향

이정원(Joung-Won Lee) 한국식품영양과학회 1991 한국식품영양과학회지 Vol.20 No.4

에탄올의 급성적 및 만성적 투여가 흰쥐의 간 및 소뇌 중의 glutathione(GSH) 양상과 지질과산화물 수준에 미치는 영향을 알아 본 결과는 다음과 같다. 간 조직에서는, 만성적 에탄올 투여(6~9g/㎏, per day, 10% 음료수로서, 4주간)에 의해 총 GSH 농도가 14.5% 저하되었고, 산화형 GSH(GSSG)는 변화가 없었으며, 따라서 GSSG/총 GSH 비율은 증가하였다. 그러나 지질과산화 수준은 변함이 없었다. 급성적 에탄올 투여에 의해 간 조직 중 지질과산화 수준이 상승되고 총 GSH 농도가 감소함은 이미 보고 되고 있다. 본 실험에서는 이와 관련시켜 급성적 에탄올 투여(50mmole/㎏, i.p.)후 post-hepatic 혈장 중의 총 GSH 수준을 측정한 결과 현저히 상승하였다. 이러한 GSH의 간에서 혈액으로의 유출은, GSH의 항산화적 소모 이외에도, 급성적 그리고 아마도 만성적인 에탄올 투여에 의한 간 조직 중의 총 GSH의 감소의 한 가능한 원인으로서 간접적으로나마 추정될 수 있겠다. 소뇌에서는 급성적 에탄올 투여는 지질과산화 수준을 증가시켰으나 GSH 양상은 변화시키지 않았으며, 만성적인 경우엔 모두 변동시키지 못하였다. The effects of acute and chronic ethanol administration on hepatic and cerebellar glutathione (GSH) statuses and lipid peroxide levels in rats were investigated. In the liver, chronic ethanol feeding (6-9 g/㎏, per day) as 10% (v/v) drinking water for 4 weeks produced a slight decrease of total GSH and an increase in the ratio of GSSG/total GSH without change of GSSG (oxidized GSH). Lipid peroxide level however was not modified. Many other studies have shown the acute ethanol loading effect in the rat liver, that is, moderate decrease of total GSH and elevation of lipid peroxide level. Relating to this, it was observed that total GSH in the plasma obtained from post-hepatic inferior vena cava was increased by acute ethanol injection (50 mmol/㎏, i.p.). This increased hepatic efflux of GSH into blood, in addition to the promoted antioxidative utilization of GSH, could be suggested as one of the possible reasons for the decrease of hepatic GSH induced by ethanol load. In the cerebellum, acute ethanol load did not change the total GSH and GSSG, but increased the lipid peroxidation rate. In the chronic, neither GSH pattern nor lipid peroxidation rate was changed.

산란계 밀집지역의 산란저하성 질병에 관한 연구

이정원 ( Jeoung Won Lee ),엄성심 ( Sung Shim Eum ),박인규 ( In Gyu Park ),배정준 ( Joung Jun Bea ),정동석 ( Dong Suk Joung ),송희종 ( Hee Jong Song ) 한국가축위생학회 2005 韓國家畜衛生學會誌 Vol.28 No.2

Newcastle disease(ND), infectious bronchitis(IB), low pathogenic avian influenza(LPAI) and fowl typhoid(FT) have been known as egg drop laying diseases because of the serious layer damage from mass zone layer. In this study, such egg drop laying diseases were investigated. To access this study, we performed to evaluate antibody titers in serum and isolated bacteria and virus from organs and feces on May, July and September in 2003. The distribution of ND from January to May, IB and LPAI from October to February of the next year, and FT from March to September were inspected by the question survey in 21 farms. ND revealed to be positive rates of 490 to 474(96.7%) in May, 510 to 506(99.2%) in July and 510 to 510(100%) in September with hemagglutination inhibition(HI) test. The mean antibody titers were 10.2, 9.9 and 10.2, respectively. With regard to IB, 484 out of 490 samples(98.7%) in May, 508 of 510(99.6%) in July and 509 of 510(99.8%) in September showed positive results and the mean antibody titers were gradually increased with 8.2, 9.0 and 9.4, respectively. According to HI test of LPAI, the positive results were shown in 442 of 480(92.1%), 394 of 494(79.8%) and 402 of 483(83.2%) in May, July and September, respectively. The mean antibody titers were decreased with 4.6, 4.3 and 4.0. The distribution of LPAI also elicited the positive rates of 480 to 475(99.0%) in May, 494 to 485(98.2%) in July, 483 to 472(97.7%) in September as determined by ELISA and the mean S/P ratio were 2.319, 2.557 and 2.380, respectively. Compared ELISA results with HI test of LPAI the positive results were 480 to 422(92.1%), 475(99.0%), 494 to 394 (79.8%), 485(98.2%) and 483 to 402(83.2%), 472(97.7%). Therefore, the positive rate determined by ELISA was higher than that of HI test with 6.9, 18.4 and 14.5%, respectively. When performed RT-PCR for ND using organ and feces samples, the pathotypes were detected 5(15.6%) in May, 2(5.3%) in July, 2(7.1%) in September but there is no samples showing positive band for LPAI. In attempt to isolate Salmonella gallinarum, bacteria were obtained from 4 cases(12.5%) in May, 9(23.6%) in July, 5(17.8%) in September. Thus the highest rate for isolation revealed to be shown in July. When evaluated the antimicrobial susceptibility to 18 isolated strains of S. gallinarum, bacteria were sensitive to trimethoprim/sulfamethox(61.1%), kanamycin(55.5%), ampicillin(55.5%) and amoxacillin/clavulanic acid(55.5%), cephalothin(50.0%), but resistant to penicillin(88.9%), streptomycin(88.9%), erythromycin(83.4%) and tetracycline(61.1%). According to HI test of ND and LPAI using captured 164 wild Korean tree sparrows(Passer nontanus), the positive rates were 47.6% and 57.3%, and the mean HI titers were 5.32 and 4.02, respectively. 71(43.2%) and 58(35.3%) in captured sparrows also showed more than 4 titers for HI test to ND and LPAI, respectively. However, the attempt for isolation of viruses failed in all samples.

칼슘의 보충섭취가 식이 나트륨 섭취 수준에 따른 혈압 변동에 미치는 영향

이정원(Lee Joung-Won),이은양(Lee Eun-Yang),이보경(Lee Bo-Kyung) 韓國營養學會 1999 Journal of Nutrition and Health Vol.32 No.6

Clostridium perfringens 감염에 의한 한우 집단 폐사 증례

이정원 ( Joung Won Lee ),김지영 ( Ji Young Kim ),곽길한 ( Kil Han Kwak ),육현수 ( Hyun Su Yuk ),손구례 ( Ku Rye Shon ),고원석 ( Won Seuk Koh ) 한국가축위생학회 2011 韓國家畜衛生學會誌 Vol.34 No.1

This is a case report that 24 heads of cattle suddenly died without clinical signs in a Hanwoo farm. The cause of death was Clostridium perfringens enterotoxemia resulted from them with feeding leftover food. The clinical signs were observed just before the death; increase of heart rate, shallow and rapid respiration, amyostasia, spasm and so forth. In autopsy, blood coagulation disorder, a little abdominal inflation, hepatomegaly and different size of red spots, congestion hemorrhage and undercurrent of bloody exudation were observed in the entire parts of small and large intestines. C. perfringens were isolated from the substantive organs, and a unique fragment of 405bp C. perfringens was amplified by PCR. Therefore, this case was diagnosed as enterotoxemia caused by α-toxin of C. perfringens A type.

비타민 C의 만성적 과량투여가 흰쥐의 혈액과 간의 지질성상, 과산화상태 및 혈소판 Thromboxane A₂ 생성에 미치는 영향

이정원(Lee Joung-Won),김소영(Kim So-Young),곽충실(Kwak Chung-Shil) 韓國營養學會 1997 Journal of Nutrition and Health Vol.30 No.6