http://chineseinput.net/에서 pinyin(병음)방식으로 중국어를 변환할 수 있습니다.
변환된 중국어를 복사하여 사용하시면 됩니다.
- 中文 을 입력하시려면 zhongwen을 입력하시고 space를누르시면됩니다.
- 北京 을 입력하시려면 beijing을 입력하시고 space를 누르시면 됩니다.
RISS 인기검색어
- 검색키워드
- 저자 : 이재화(Jae Hwa, Lee) (검색결과 257 건)
- 무료
- 기관 내 무료
- 유료
-
악성 근상피종의 세침흡인 세포학적 소견 -1 예 보고-
이재화,박진경,허방,Lee, Jae-Hwa,Park, Jean-Kyung,Hur, Bang 대한세포병리학회 2002 대한세포병리학회지 Vol.13 No.1
Malignant myoepithelioma (myoepithelial carcinoma), is a very rare malignant epithelial accounting for less than 1% of all salivary gland tumors and has an intermediate malignant potential. We report a case of malignant myoepithelioma arising in the left parotid giand in a 54-year-old man, which was difficult to differentiate from pleomorphic adenoma and other malignant salivary gland neoplasms. Fine needle aspiration cytology of the parotid gland showed cellular smear, composed of overlapped sheets and clusters or individually scattered tumor cells without any acinic or ductal structures. The tumor cells were rather uniform, with distinct cell borders and moderate amount of cytoplasm. The eccentrically located nuclei were oval to round and pleomorphic and showed prominent nucleoli. A few clear cells were noted in the cellular aggregates Metachromatic matrix was seen between individual tumor cells in a lacelike fashion, resembling pleomorphic adenoma. According to the immunohistochemical staining, we recognized that the component cells are myoeplthelial in nature, showing reactivity for the S-100 protein, vimentin, and actin.
-
형질전환된 벼세포배양에서 green fluorescent protein (GFP) 생산
이재화,Lee, Jae-Hwa 한국생명과학회 2007 생명과학회지 Vol.17 No.2
형광단백질 (green fluorescent protein, GFP)은 생물공정을 살피데 지표 단백질로 유용하게 사용이 된다. 본 연구에서는 쌀세포에서 외래 단백질의 발현양상을 관찰하기 위해서, 표지 단백질로 GFP를 형질전환 후 이것에서 유도된 현탁세포에서 GFP의 발현 양상을 관찰하였다. 형질전환시 GFP의 발현을 위한 프로모터로 RAmysE를 사용하였으며 이것은 배양액 중에서 당이 고갈되었을 때 강력히 작동된다. 그래서 본 연구에서는 배양액 중에 다양한 슈크로오스 농도로 쌀세포를 배양하여 세포의 성장양태 및 GFP의 발현양에 미치는 영향을 관찰한 결과 세포의 성장은 12%의 당농도에서 7.06g/L로 최적이였으며 GFP는 당을 가장 적게 사용한 3%에서 최적임을 알 수가 있었다. 이것은 세포의 성장과 GFP의 생산에 사용된 당이 반대로 영향을 미침을 알 수가 있었으며 향후 최적의 대량배양을 위해서는 세포의 성장과 산물의 생산시기를 분리한 이단계 배양법이 필요함을 암시한다. Green fluorescent protein (GFP) is an attractive reporter for bioprocess monitoring. A fluorescence-based method was developed to quantify GFP levels in transgenic plants and protein extracts. In this study, GFP was produced and secreted from suspension cells derived from transgenic rice. The RAmy3E promoter placed before the GFP gene controlled by sugars such as sucrose. The effects of sucrose concentration on the secretion of GFP and total protein into the medium were investigated in batch suspension culture. It was possible, therefore, to induce the expression of the GFP by removing sucrose from the cultured media or by allowing the rice suspension cells to deplete sucrose catabolically. The dry cell weight (7.06 g/L) and GFP level were detected as highest at 12%, 3% sucrose after 20 day culture, respectively. However secreted GFP fluorescence at the other sucrose concentrations (6%, 12%, 18% and 24%) were a little amount in media.
-
형질전환된 식물세포에서 hGM-CSF 생산과 안정성에 대한 다양한 탄소원의 효과
이재화,Lee Jae-Hwa 한국식물생명공학회 2005 식물생명공학회지 Vol.32 No.4
The effects of various carbon sources on the secretion of hGM-CSF, total protein and protease into the medium were investigated in transgenic tobacco cells. The dry cell weight (11.2 g/L) and wet cell weight (310.8 g/L) were highest at 30 g/L glucose after 5-day culture but, the dry cell weight (13.4 g/L) and wet cell weight (480 g/L) were highest at 30 g/L sucrose after 10-day culture. The total protein (110.3 mg/L), protease activity (3950 U/L) and total secreted hGM-CSF (56 mg/L) were highest at 30 g/L sucrose after 10-day culture. Stabilization of the total secreted protein and hGM-CSF in various carbon source concentrations was determined. Total secreted protein was most stabilized in the medium containing sucrose. However, the loss of the total protein was increased with the concentrations of high level in medium containing sorbitol, mannitol, fructose, and glucose. hGM-CSF was more stabilized in the medium containing sucrose than in the medium containing sorbitol, mannitol, fructose, glucose. 다양한 탄소원을 사용하였을 시, 세포성장에 미치는 영향은 배양 5일제 glucose를 사용한 배양세포에서 dry cell weight가 11.2 g/L, wet cell weight가 310.8 g/L로 가장 높았다. 이후 배양 10일차에는 오히려 sucrose를 탄소원으로 사용한 배양세포에서 dry cell weight가 13.4 g/L, wet cell weight가 480 g/L로 가장 높이 성장한 것을 확인할 수 있었다. Total secreted protein의 경우는 배양 10일차에 sucrose를 기본 탄소원으로 사용하였을 때 110.3 mg/L로 가장 높게 나왔다. Total secreted protease 역시, 배양 10일차에 sucrose를 기본 탄소원으로 사용한 배양씩에서 3950 U/L로 가장 많은 양이 측정되었다. 최종적으로 재조합된 단백질인 hGM-CSF의 생산량에 대한 측정결과, sucrose를 기본 탄소원로 사용한 배지에서 배양 10일차에 56 mg/L로 가장 높게 측정됨을 확인할 수 있었다. 이 외에 다양한 탄소원 농도변화에 따른 total secreted protein과 hGM-CSF의 안정성 평가를 확인해본 결과, total secreted protein의 경우, 비교대상으로 사용한 sucrose에서는 농도변화에도 큰 손실률을 보이지는 않았으나, sorbitol, mannitol, fructose, glucose의 경우에는 농도가 높아질수록 배양액내의 protein의 손실률이 증가 하였다. 총 분비된 hGM-CSF 역시 sucrose에 비해 sorbitol, mannitol, fructose, glucose에서는 분비된 hGM-CSF의 양이 크게 감소하는 것을 확인할 수 있었고, 탄소원의 농도가 높아질수록 hGM-CSF의 양이 감소하는 경향이 크게 나타났다.
-
-
-
직접수치모사를 이용한 난류경계층 내의 거대난류구조 연구
이재화(Jae Hwa Lee),성형진(Hyung Jin Sung) 대한기계학회 2009 大韓機械學會論文集B Vol.33 No.12
Direct numerical simulation (DNS) of a turbulent boundary layer with moderate Reynolds number was performed to scrutinize streamwise-coherence of hairpin packet motions. The Reynolds number based on the momentum thickness (θin) and free-stream velocity (U∞) was varied in the range Reθ=1410~2540 which was higher than the previous numerical simulations in the turbulent boundary layer. In order to include the groups of hairpin packets existing in the outer layer, large computational domain was used (more than 50δo, where δo is the boundary layer thickness at the inlet in the streamwise domain). Characteristics of packet motions were investigated by using instantaneous flow fields, two-point correlation and conditional average flow fields in xy-plane. The present results showed that a train of hairpin packet motions was propagating coherently along the downstream and these structures induced the very large-scale motions in the turbulent boundary layer.
-
이재화(Jae Hwa Lee),이승현(Seung-Hyun Lee),김경연(Kyoungyoun Kim),성현진(Hyung Jin Sung) 대한기계학회 2008 大韓機械學會論文集B Vol.32 No.6
Turbulent coherent structures near rod-roughened wall are investigated by analyzing the database of directnumerical simulation of turbulent boundary layer. The surface roughness rods with the height k/δ =0.05 are arranged periodically in Reδ = 9000 . The roughness sublayer is defined as two-point correlations are not independent of streamwise locations around roughness. The roughness sublayer based on the two-point spatial correlation is different from that given by one-point statistics. Quadrant analysis and probability-weighted Reynolds shear stress indicate that turbulent structures are not affected by surface roughness above the roughness sublayer defined by the spatial correlations. The conditionally-averaged flow fields associated with Reynolds shear stress producing Q2/Q4 events show that though turbulent vortices are affected in the roughness sublayer, these are very similar at different streamwise locations above the roughness sublayer. The Reynolds stress producing turbulent vortices in the log layer ( y /δ = 0.15) have almost the same geometrical shape as those in the smooth wall-bounded turbulent flows. This suggests that the mechanism by which the Reynolds stress is produced in the log layer has not been significantly affected by the present surface roughness.
-
직접수치모사를 이용한 난류경계층 내의 거대난류구조 연구
이재화(Jae Hwa Lee),성형진(Hyung Jin Sung) 대한기계학회 2009 대한기계학회 춘추학술대회 Vol.2009 No.5
Direct numerical simulation (DNS) of a turbulent boundary layer with moderate Reynolds number was performed to scrutinize streamwise-coherence of hairpin packet motions. The Reynolds number based on the momentum thickness (θ<SUB>in</SUB>) and free-stream velocity (U,) was varied in the range Re?=1410~2540 which was higher than the previous numerical simulations in the turbulent boundary layer. In order to include the groups of hairpin packets existing in the outer layer, large computational domain was used (more than 50δ<SUB>o</SUB>, where δ<SUB>o</SUB> is the boundary layer thickness at the inlet in the streamwise domain). Characteristics of packet motions were investigated by using instantaneous flow fields, two-point correlation and conditional average flow fields in xy-plane. The present results showed that a train of hairpin packet motions was propagating coherently along the downstream and these structures induced the very large-scale motions in the turbulent boundary layer.
-
-
연관 검색어 추천
이 검색어로 많이 본 자료
활용도 높은 자료
