도인승기탕에 의한 당뇨병성 혈관장애 개선효과

이윤정,김언국,김혜윰,윤정주,이소민,이용표,이건목,강대길,이호섭,Lee, Yun Jung,Kim, Eon Kuk,Kim, Hye Yoom,Yoon, Jung Joo,Lee, So Min,Lee, Yong-Pyo,Lee, Geon Mok,Kang, Dae Gill,Lee, Ho Sub 대한한의학방제학회 2013 大韓韓醫學方劑學會誌 Vol.21 No.1

Objectives : Diabetes mellitus is the leading cause for vascular complications such as atherosclerosis. The present study is to investigate whether Doinseunggi-tang (DST) improves diabetic vascular dysfunction in type II diabetes. Methods : The db/db mice were treated with high fat/high cholesterol diet and DST (200 mg/kg/day) for 8 weeks. Results : DST significantly lowered blood glucose and systolic blood pressure. In addition, DST also markedly decreased total plasma cholesterol, triglyceride, and LDL-cholesterol, whereas increased the HDL-cholesterol. Vascular relaxation of aortic rings by acetylcholine or SNP was ameliorated by DST in a dose-dependent manner. Damage of vascular intima and hypertrophic of media was improved by DST. Immunohistological study revealed that DST attenuated the increase of ICAM-1, VCAM-1, and ET-1 expression in thoracic aorta. Conclusions : Taken together, DST suppressed hyperglycemia and diabetic vascular dysfunction in type II db/db mice. The present data suggests that Doinseunggi-tang may be prevent a development of diabetic atherosclerosis.

Salmonella typhimurium 에서 무산소 환경 유도유전자들에 대한 조절유전자들의 특성 및 클로닝

이윤정,방일수,이인수,이영록,박용근 ( Yun Joung Lee,Iel Soo Bang,In Soo Lee,Yung Nok Lee,Yong Keun Park ) 한국환경생물학회 1993 환경생물 : 환경생물학회지 Vol.11 No.2

New regulatory loci which participate in the anaerobic environment inducible gene(ani) expression in Salmonella typhimurium were identified. These regulators (oxr) showed negative regulatory effect on the anaerobiosis inducible operon fusions of this organism in aerobic environment (oxr 107 and oxr 108 to ani 2001, oxr 109 to ani 2007). They were identified as the trans-acting regulators, and increased β-galactosidase activity of ani-lacZ fusion strains up to about 10-240 fold in aerobic environment. By using the library of S. typhimurium chromosomal DNA, two negative regulators (oxr 101 and oxr 109) were cloned. The insert fragment of each clone, pPK32 for oxr 101 and pPK33 to oxr 109 were 7.8Kb and 9.3Kb, respectively. The recombinant plasmid pPK32 didn`t complement the mutation effect of oxr 101 on minimal media but rich media, pPK33 complemented the mutation effect of oxr 109 on both minimal and rich media.

고지혈증 동물모델에서 홍삼과 천마 혼합투여에 의한 혈관 염증 개선연구

이윤정,김혜윰,윤정주,이소민,고정현,이호섭,최경민,강대길,Lee, Yun-Jung,Kim, Hye-Yoom,Yoon, Jung-Joo,Lee, So-Min,Kho, Joung-Hyun,Lee, Ho-Sub,Choi, Kyung-Min,Kang, Dae-Gill 대한한의학방제학회 2012 大韓韓醫學方劑學會誌 Vol.20 No.1

Objectives : This experimental study was designed to investigate the inhibitory effects of combination with Korean red ginseng and Gastrodia rhizoma on vascular dysfunction in high-fat/cholesterol diet-induced hyperlipidemia. Methods : Sprague-Dawley rats were fed with 7.5% cocoa butter and 1.25% cholesterol for 10 weeks, with Panax ginseng (PG), and mixtures of Panax ginseng and Gastrodia rhizoma (PGM), respectively. Results : Chronic treatment with PG and PGM significantly decreased body weight. The aortic expression of cell adhesion molecules such as intracellular adhesion molecule-1 (ICAM-1), vascular cell adhesion molecule-1 (VCAM-1), and E-selectin were markedly increased in hyperlipidemia rats. Interestingly, PGM significantly decreased cell adhesion molecules expression. However, there was no significant decrease in PG group. In addition, PG and PGM group inhibited high-fat/cholesterol diet-induced cytokine such as monocyte chemoattractant protein (MCP-1) mRNA expression. Furthermore, PG and PGM group significantly decreased c-reactive protein protein (CRP) level. Especially, PGM significantly accentuated the decrease of MCP-1 mRNA expression and CRP level. Conclusions : the present study provides an evidence that combination with Panax ginseng and Gastrodia rhizoma enhances anti-vascular protective effects through suppression of vascular inflammation in hyperlipidemic rats.

LPS에 의한 급성 폐손상에서 게르마늄에 의한 폐포-모세혈관 장벽의 변화

이윤정,조현국,신건호,전경희,Lee, Yoon-Jeong,Cho, Hyun-Gug,Sin, Gun-Ho,Jeune, Kyung-Hee 한국현미경학회 2009 Applied microscopy Vol.39 No.1

급성호흡곤란증후군은 다양한 원인에 의해 발생하는 급성 염증성 폐질환으로써 폐포-모세혈관 장벽의 파괴로부터 기인하며 파괴 기전에 호중구가 중요한 역할을 담당한다. 이에 대한 게르마늄의 항염증 효과 즉, 호중구의 폐조직 내 유주 억제로 인한 폐포-모세혈관의 손상의 감소로 폐손상이 경감되는지를 확인하고자 하였다. 실험군은 생리식염수를 투여한 대조군(CON), 내독소 5시간 투여군(LPS), 그리고 게르마늄 1시간 전처리 후 내독소 5시간 투여군(Ge+LPS)으로 나누었으며, 내독소는 $100{\mu}g$을 0.5 mL 생리식염수에 녹여 기도내로 분무하고, 게르마늄은 체중 100 g당 26 mg을 복강으로 투여하였다. 내독소의 주입으로 급성 폐손상을 유도시킨 내독소 투여군에서는 대조군과 비교하여 폐부종(p<0.001), 기관지폐세척액 내 단백질 함량(p<0.05), 호중구의 침윤(p<0.001)이 현저히 증가하였으나 게르마늄을 전처리 한 군은 내독소 투여군과 비교하여 모든 항목에서 유의하게 감소하였다. 현미경을 통한 관찰 결과에서도 게르마늄 전처리군은 내독소 투여군에 비해 내피세포, 제I형 폐포세포, 기저판으로 구성된 폐포-혈관장벽의 구조가 비교적 잘 보존되었고, 제II형 폐포세포의 Lamellar body와 미세융모 그리고 기저판의 구조도 비교적 정상적으로 보존된 상태로 나타났다. 그러므로 게르마늄은 항염증물질로 작용, 즉 호중구의 폐조직 내로의 유주현상을 방해하여 활성화된 호중구의 독성물질로 인한 폐포-모세혈관의 손상을 감소시킬 수 있었으므로 결과적으로 내독소로 유도된 폐손상은 게르마늄의 전처리에 의해 억제되었다고 판단된다. Acute respiratory distress syndrome (ARDS), also known as an acute inflammatory lung disease is developed by various factors that is originated from the destruction of alveolar-capillary barrier, and neutrophils plays an important role in the destruction. The study intended to confirm, the anti-inflammatory effect of germanium, whether a lung injury has been mitigated with the reduction of injury in alveolar-capillary barrier resulting from inhibition of neutrophils migration in lung tissue. Test groups were divided in saline administered CON, 5 hours of endotoxin administered LPS and 5 hours of endotoxin administered Ge+LPS following 1 hours of pre-processed germanium. $100{\mu}g$ endotoxin was melted in 0.5 mL saline and sprayed into airway and 26 mg germanium per 100 g weight was administered into abdominal cavity. The endotoxin group which induced an acute lung injury with administered endotoxin showed dramatic increase of pulmonary edema (p<0.001), protein contents in bronchoalveolar lavage fluid, BALF (p<0.05) and neutrophils of infiltration in BALF (p<0.001) comparing with a control group, while a pre-treated germanium group showed significant decrease in all categories comparing to the endotoxin administerd group. In the result of a microscopic observation, the structure of alveolar-capillary barrier which is constructed with basal lamina, alveolar type I cells and endothelial cell were preserved of the pre-treated germanium group relatively well compare to the endotoxin administered group. And the construction of lamellar body, microvilli and basal lamina of alveolar type II cells were also preserved relatively well. Hence, germanium activates as an anti-Inflammatory mediator in other words, it interfered neutrophils migration into lung tissue, thereby reduced injury of alveolar-capillary barrier from toxic substances of activated neutrophils. Consequently, the study has determined that the acute lung injury induced by endotoxin has been decreased by the pre-treated germanium.

위눈꺼풀 성형술 후 눈꺼풀계측치의 변화

이윤정,강일봉,김준현,Yoon Jung Lee,Il Bong Kang,Joon Hyun Kim 대한안과학회 2007 대한안과학회지 Vol.48 No.3

Purpose: To evaluate the morphological changes in the external eyes after upper blepharoplasty. Methods: Twenty-six eyes of 13 patients undergoing upper blepharoplasty from May 2002 to May 2003 were selected. All surgeries were performed by one surgeon. MRD1, MRD2, interpalpebral fissure height, and levator function test were each measured twice, and the averages were calculated. Likewise, significant changes were checked before and after the operation. The subjects were divided into two groups. For group A (n=12), a double line suture was stitched at the levator aponeurosis, which was directly superior to the tarsal plate. In group B (n=14) the suture was stitched at a levator aponeurosis 3 mm superior to tarsal plate. The delta levator function (postoperative mean levator function minus preoperative mean levator function) measurements were calculated and compared between the two groups. Results: The MRD1 was 2.04±0.75 (mean±SD) before the operation, and 2.0±0.81 after the operation. MRD2 was 5.23±0.75 before the operation, 5.35±0.54 after operation. Interpalpebral fissure height was 7.27±0.38 before the operation and 7.35±0.63 after the operation. There were no statistically significant factors before and after the operation in MRD1, MRD2 and interpalpebral fissure height. The levator function was 14.04±1.80 before versus 16.19±1.58 after. This increase was statistically significant (p<0.01, Wilcoxon signed ranks test). The delta levator function was 1.58±0.90 for group A and 1.96±1.36 for group B. Conclusions: After upper blepharoplasty, the measurement of levator function increased significantly.

산후우울증 환자에서 혈장 Cytokine의 농도변화에 대한 전향적 연구

이윤정,김용구,김계현,이분희,Lee, Younjung,Kim, Yong-Ku,Kim, Kye-Hyun,Lee, Bun-Hee 한국정신신체의학회 2020 정신신체의학 Vol.28 No.2

연구목적 산후우울증은 일반적으로 산모의 10~15%에서 발병하는 질환으로 그 원인으로는 정신사회적인 요인과 산과적인 요인이 모두 관계 있다고 알려져 있다. 감염, 손상, 악성종양, 자가면역질환, 스트레스에 의해 면역체계가 자극되면 proinflammatory cytokine과 anti-inflammatory cytokine이 생성되고 분비된다고 알려져 있다. 본 연구에서는 산후우울증이 있는 경우 산후우울증이 없는 군에 비해 혈중 Transforming growth factor-β1(TGF-β1), Insulin-like growth factor-1 (IGF-1), β-Nerve growth factor (β-NGF), Interleukin-2 (IL-2), IL-4, IL-6, Tumor necrosis factor-α (TNF-α), Interferon-γ (IFN-γ)의 농도가 상승되어 있을 것이라 가정하고, 임산부에서의 우울증의 경과에 따라 cytokine의 농도의 변화를 알아보고자 한다. 방 법 본 연구는 총 104 명의 임산부와 60명의 임신을 하지 않은 정상 대조군을 대상으로 하였다. 우울 증상은 임신 24주, 출산 1주, 출산 6주에 에딘버러 산후 우울 척도(EPDS)를 사용하여 평가하였다. EPDS의 총 점수가 10 이상인 경우, 우울증상이 있는 것으로 판단하였으며 EPDS 점수 변화에 따라 임산부 정상대조군, 출산 후 우울-회복군, 출산 후 우울군 세 그룹으로 나누었다. 결 과 임신군과 비임신 정상대조군을 비교하였을 때 TGF-β1, IGF-1의 혈장 농도는 임신군에서 비임신 정상대조군보다 더 높았다(TGF-β1 ; p<0.01, IGF-1 ; p=0.026). 그러나 β-NGF, IL-2, IL-4, IL-6, IFN-γ, TNF-α는 비임신 여성대조군에 비해 임산부 정상대조군에서 그 농도가 낮게 측정되었다(β-NGF ; p=0.001, IL-2 ; p<0.01, IL-4 ; p<0.01, IL-6 ; p<0.01, IFN-γ ; p<0.01, TNF-α ; p<0.01) 임신 24주에 TGF-β1, IGF-1, β-NGF, IL-2, IL-4, IL-6, IFN-γ, TNF-α의 농도를 살펴보면 임산부 정상대조군, 출산 후 우울-회복군, 출산 후 우울군 세 군간에 유의한 차이가 없었으며 출산 6주에도 역시 유의한 차이가 관찰되지 않았다. 또한 임신 24주, 출산 6주에 시간에 따른 농도 차이를 비교해 보았을 때 세 군에서 모두 그 통계적으로 유의한 차이는 없었다. 결 론 본 연구는 비임신 정상대조군과 임신군간의 혈장 cytokine 농도에서 유의한 차이를 발견했으나 산후우울증군과 정상 임신대조군간의 혈장 cytokine 농도는 유의한 차이를 밝혀내지는 못했다. Objectives : Postpartum depression is known to occur in 10-15% of mothers. The concentration of cytokine varies depending on stress, depression, pregnancy and general medical conditions. We hypothesized that the concentration of cytokines may be related to reproduction and childbirth, and that women with postpartum depression would show alterations in cytokines levels. Methods : A total of 104 pregnant women were selected as subjects, and 60 non-pregnant women were selected as normal controls. Symptoms of depression were evaluated in the pregnant study subjects using the diagnostic criteria outlined in the Edinburgh Postnatal Depression Scale (EPDS). The pregnant subjects were divided into three groups perinatal non-depression controls (n=61), postpartum depression-recovery (n=18), and postpartum depression (n=25). Results : The plasma concentration of TGF-β1, IGF-1 was higher in the pregnant group than in non-pregnant controls (TGF-β1 ; p<0.01, IGF-1 ; p=0.026). At 24 weeks of pregnancy and 6 weeks of delivery, there were no significant differences in the plasma concentration of TGF-β1, IGF-1, β-NGF, IL-2, IL-4, IL-6, IFN-γ, TNF-α between the three groups. There was no statistically significant difference in all three groups during the course of depression in pregnant women. Conclusions : This study found significant difference in plasma cytokines concentrations between non-pregnant controls and perinatal non-depression controls.

인수공통전염병(13) - 조류인플루엔자

이윤정,Lee, Yun-Jeong 대한수의사회 2008 대한수의사회지 Vol.44 No.4

유지요소의 크기와 형태가 간접복합레진과 금속간의 결합강도에 미치는 영향

이윤정,전영찬,정창모,Lee, Yun-Jung,Jeon, Young-Chan,Jeong, Chang-Mo 대한치과보철학회 2007 대한치과보철학회지 Vol.45 No.5

Purpose: The purpose of this study was to investigate the effect of sire and shape of retention element on the bond strength of indirect composite resin and metal. Material and method: The metal disk specimens, each 6mm in diameter, were cast from CrCo alloy. They were divided into 8 groups by applied retention element. retention bead group $B2\;({\phi}\;0.2mm),\;B4\;({\phi}\;0.4mm),\;B6\;({\phi}\;0.6mm),\;B8\;({\phi}\;0.8mm)$, retention crystal group C2 (0.2mm), C5 (0.5mm), C8 (0.8mm) and sandblasting group SB ($110{\mu}m\;Al_2O_3$ blasting) as control. Eighty-eight metal specimens were veneered with $TESCERA^{(R)}$ Indirect resin system. One specimen of each group was sectioned and the resin-metal bonding pattern at the interface was observed under measuring microscope. Other specimens were then tested for tensile bond strength on an Instron universal testing machine at a crosshead speed of 2mm/min. Results: 1. Compared to sandblasting, beads or crystals increased the resin-metal bond strength (P<.05). 2. 0.2mm retention crystals were most effective in improving the resin-metal bond strength (P>.05). 3. 0.2mm beads showed the highest bond strength among retention bead groups, but there was no statistically significant difference (P>.05). 4. Retention crystals tend to be higher in bond strength than retention beads due to wider surface area. 5. The larger retention element, the larger the undercut for the mechanical retention, but the gap at resin-metal interface was also increased. Conclusion: Within the limitations of this study, 0.2mm retention crystals were most effective in improving the resin-metal bond strength.

건물 에너지 분석의 개념과 활용

이윤정,김상훈,전준용,Lee, Yun-Jeong,Kim, Sang-Hun,Jeon, Jun-Yong 한국건설관리학회 2012 한국건설관리학회 논문집 Vol.13 No.3

비중격만곡증 환자의 눈물주머니코안연결술의 임상예후

이윤정,문상원,조희윤,정진혁,Yoon Jung Lee,M,D,Sang-Won Moon,M,D,Hee Yoon Cho,M,D,Jin-Hyeok Jeong,M,D 대한안과학회 2008 대한안과학회지 Vol.49 No.5

Purpose: Septal deviation has few subjective symptoms. However, a difficulty remains in doing DCR (dacryocystorhinostomy) in these patients. We investigate the clinical course of DCR in patients with a septal deviation. Methods: Acquired nasolacrimal duct-obstructed patients who had undergone DCR with septal deviation from 2003 to 2006 in our hospital were retrospectively analyzed. We measured the septal distance using paranasal computed tomography taken preoperatively and compared the sex and age of patients, the kinds of operations, outcome of surgery, and ocular and nasal complications in our analysis. Results: Twenty-two eyes of 22 patients with a mean age of 55.3 years (range 29~74) were analyzed (4 males, 18 females). Mean septal distance was 4.84 mm (range 2.18~6.99 mm). Nineteen eyes had endonasal DCR, and three had external DCR. Two had DCR combined with septoplasty. Three had septoplasty as a secondary operation, Nasal complication was synechiae in 9 eyes. DCR success was seen in 15 eyes, and DCR failure in 7 eyes. Septal distance was not correlated with DCR success but was correlated with nasal complication (Mann-Whitney test, p=0.003) Conclusions: In the case of DCR with septal deviation, the surgical technique used will need to minimize the mucosal injury and ensure appropriate nasal correction to prevent future complications.